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EC Tree
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
beta-n-acetylgalactosaminidase, n-acetyl-beta-d-galactosaminidase, n-acetyl-beta-galactosaminidase, n-acetylgalactosaminidase, beta-d-n-acetylgalactosaminidase,
more
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beta-acetylgalactosaminidase
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beta-D-N-acetylgalactosaminidase
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beta-N-acetylgalactosaminidase
N-acetyl-beta-D-galactosaminidase
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N-acetyl-beta-galactosaminidase
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N-acetylgalactosaminidase
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Bacillus beta-GlcNAcase
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Bacillus beta-GlcNAcase
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beta-N-acetylgalactosaminidase
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beta-N-acetylgalactosaminidase
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beta-N-acetylgalactosaminidase
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beta-N-acetylgalactosaminidase
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(GalNAcbeta(1-4))n + H2O = (GalNAcbeta(1-4))n-1 + 2-(acetylamino)-2-deoxy-beta-D-galactopyranose
(GalNAcbeta(1-4))n + H2O = (GalNAcbeta(1-4))n-1 + 2-(acetylamino)-2-deoxy-beta-D-galactopyranose
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(GalNAcbeta(1-4))n + H2O = (GalNAcbeta(1-4))n-1 + 2-(acetylamino)-2-deoxy-beta-D-galactopyranose
NgaP adopts substrate-assisted catalysis in which residues Glu608 and Asp607 could function as a proton donor and a stabilizer of the 2-acetamide group of the beta-GalNAc at the active site, respectively
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hydrolysis of O-glycosyl bond
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beta-N-acetyl-D-galactosaminide N-acetylgalactosaminohydrolase
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4-methylumbelliferyl beta-N-acetylgalactosaminide + H2O
4-methylumbelliferone + N-acetyl-beta-D-galactosamine
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?
4-methylumbelliferyl-beta-D-N-acetylgalactosaminide + H2O
4-methylumbelliferone + N-acetyl-beta-D-galactosamine
4-nitrophenyl beta-acetylgalactosaminide + H2O
4-nitrophenol + beta-acetylgalactosamine
4-nitrophenyl beta-N-acetylglucosaminide + H2O
beta-N-acetylglucosamine + 4-nitrophenol
1-3% of the activity with 4-nitrophenyl beta-N-acetylgalactosaminide
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?
4-nitrophenyl N-acetyl-beta-D-galactosaminopyranoside + H2O
N-acetyl-D-galactosamine + 4-nitrophenol
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?
4-nitrophenyl-N-acetyl-beta-D-galactosaminide + H2O
4-nitrophenol + N-acetyl-beta-D-glucosamine
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activity measured in whole fly homogenates, substrate concentration of 6 mM in citrate buffer, flies of arid regions tend to produce more beta-N-acetylgalactosaminidase than those originating in sugar-rich environments
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?
beta-N-acetylgalactosaminylated oligosaccharides + H2O
?
beta-phenyl-N-acetylgalactosamines + H2O
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substrate specificity 85%
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?
GalNAc-beta-1,3-Gal-alpha-1,4-Gal-beta-1,4-Glc-beta-1,1-Cer + H2O
GalNAc + Gal-alpha-1,4-Gal-beta-1,4-Glc-beta-1,1-Cer
i.e. globotetraosylceramide, 39% of the activity with GalNAc-1,4-Gal-1,4-Glc-1,1-Cer
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?
GalNAc-beta-1,4-Gal-beta-1,4-Glc-beta-1,1-Cer
GalNAc + Gal-beta-1,4-Glc-beta-1,1-Cer
i.e. asialo-GM2
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?
N-acetyl-beta-D-galactosaminides + H2O
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N-acetyl-beta-D-galactosaminides + H2O
N-acetyl-D-galactosamine + an alcohol
p-nitrophenyl beta-N-acetylgalactosaminide + H2O
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additional information
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enzyme cleaves the nonreducing terminal beta-GalNAc residues of gangliotriaosylceramide and globotetraosylceramide. Enzyme prefers the GalNAc-1,4-linkage over the GalNAc-1,3-linkage
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4-methylumbelliferyl-beta-D-N-acetylgalactosaminide + H2O
4-methylumbelliferone + N-acetyl-beta-D-galactosamine
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4-methylumbelliferyl-beta-D-N-acetylgalactosaminide + H2O
4-methylumbelliferone + N-acetyl-beta-D-galactosamine
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4-nitrophenyl beta-acetylgalactosaminide + H2O
4-nitrophenol + beta-acetylgalactosamine
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4-nitrophenyl beta-acetylgalactosaminide + H2O
4-nitrophenol + beta-acetylgalactosamine
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?
4-nitrophenyl beta-acetylgalactosaminide + H2O
4-nitrophenol + beta-acetylgalactosamine
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beta-N-acetylgalactosaminylated oligosaccharides + H2O
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beta-N-acetylgalactosaminylated oligosaccharides + H2O
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N-acetyl-beta-D-galactosaminides + H2O
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N-acetyl-beta-D-galactosaminides + H2O
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N-acetyl-beta-D-galactosaminides + H2O
N-acetyl-D-galactosamine + an alcohol
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substrate specificity towards beta-GalNAc residues is markedly higher than those of any other beta-GalNacase
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?
N-acetyl-beta-D-galactosaminides + H2O
N-acetyl-D-galactosamine + an alcohol
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substrate specificity towards beta-GalNAc residues is markedly higher than those of any other beta-GalNacase
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?
N-acetyl-beta-D-galactosaminides + H2O
N-acetyl-D-galactosamine + an alcohol
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e.g. 4-methylumbelliferyl-beta-N-acetylgalactosaminide
e.g. 4-methylumbelliferone
?
N-acetyl-beta-D-galactosaminides + H2O
N-acetyl-D-galactosamine + an alcohol
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e.g. 4-methylumbelliferyl-beta-N-acetylgalactosaminide
e.g. 4-methylumbelliferone
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p-nitrophenyl beta-N-acetylgalactosaminide + H2O
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p-nitrophenyl beta-N-acetylgalactosaminide + H2O
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p-nitrophenyl beta-N-acetylgalactosaminide + H2O
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?
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beta-N-acetylgalactosaminylated oligosaccharides + H2O
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N-acetyl-beta-D-galactosaminides + H2O
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beta-N-acetylgalactosaminylated oligosaccharides + H2O
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beta-N-acetylgalactosaminylated oligosaccharides + H2O
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N-acetyl-beta-D-galactosaminides + H2O
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N-acetyl-beta-D-galactosaminides + H2O
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?
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Mg2+
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relative activity in presence of MgSO4 10 mM 104%
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Fe2+
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enzyme activity completely inhibited in the presence of 10 mM FeSO4
Hg2+
5 mM, strong inhibition
mannose
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inhibition of beta-N-acetylgalactosaminidase but activation of alpha-N-acetylgalactosaminidase, three concentrations of 10, 50, and 100 mM tested
methyl 2,5-dideoxy-2,5-imino-D-allonamide
methyl 2,5-dideoxy-2,5-imino-D-mannonamide
methyl 2,5-dideoxy-2,5-imino-D-talonamide
methyl 2,5-dideoxy-2,5-imino-L-gulonamide
N-acetylgalactosaminide thiazoline
competitive
N-acetylglucosaminide thiazoline
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Ni2+
5 mM, strong inhibition
p-hydroxymercuribenzoate
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Urea
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relative activity in presence of 2 M 32%
Zn2+
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enzyme activity completely inhibited in the presence of 10 mM ZnSO4
Cu2+
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enzyme activity completely inhibited in the presence of 10 mM CuSO4
Cu2+
5 mM, strong inhibition
methyl 2,5-dideoxy-2,5-imino-D-allonamide
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methyl 2,5-dideoxy-2,5-imino-D-allonamide
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methyl 2,5-dideoxy-2,5-imino-D-mannonamide
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methyl 2,5-dideoxy-2,5-imino-D-mannonamide
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methyl 2,5-dideoxy-2,5-imino-D-talonamide
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methyl 2,5-dideoxy-2,5-imino-D-talonamide
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methyl 2,5-dideoxy-2,5-imino-L-gulonamide
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methyl 2,5-dideoxy-2,5-imino-L-gulonamide
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N-acetylgalactosamine
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relative activity in presence of 10 mM 50%, in presence of 50 mM 12%
N-acetylgalactosamine
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competitive inhibition
N-acetylgalactosamine
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dose dependent inhibition, at lower levels than inhibition by N-acetylglucosamine, three concentrations of 10, 50, and 100 mM tested
N-acetylgalactosamine
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N-acetylglucosamine
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no inhibition at N-acetylglucosamine concentration of 40 mM
N-acetylglucosamine
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constant inhibition, three concentrations of 10, 50, and 100 mM tested
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N-ethylmaleimide
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1mM solution increase the reaction rates 2-3fold
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Asthma
[Lysosomal leukocyte glycosidases in childhood bronchial asthma]
Brain Abscess
Production of specific glycosidase activities by Streptococcus intermedius strain UNS35 grown in the presence of mucin.
Carcinoma, Hepatocellular
Serum and host liver activities of glycosidases and sialyltransferases in animals bearing transplantable tumors.
Carcinoma, Hepatocellular
[Value of serum glycosidase spectrum in the diagnosis of hepatocellular carcinoma]
Cystinosis
A study of the low beta-galactosidase activity in cystinotic fibroblasts: effects of cysteamine.
Cysts
Giardia cyst wall-specific carbohydrate: evidence for the presence of galactosamine.
Gangliosidoses, GM2
Ganglioside GM2 N-acetyl-beta-D-galactosaminidase activity in cultured fibroblasts of late-infantile and adult GM2 gangliosidosis patients and of healthy probands with low hexosaminidase level.
Hyperthyroidism
Differential effect of hyperthyroidism on rat epididymal glycosidases.
Hyperthyroidism
Impact of altered thyroid hormone status on prostatic glycosidases.
Leukemia
Serum and host liver activities of glycosidases and sialyltransferases in animals bearing transplantable tumors.
Liver Diseases
[Value of serum glycosidase spectrum in the diagnosis of hepatocellular carcinoma]
Lymphoma
B-cell lymphoma marking only with anti-epithelial membrane antigen.
Neoplasms
Glycosyltransferase and glycosidase activities in ovarian cancer patients.
Neoplasms
Histochemical studies on N-acetyl-beta-galactosaminidase and N-acetyl-beta-glucosaminidase in the cancer of the uterine cervix.
Neoplasms
Studies on the kinetics of glycosidases from chemically-induced rat colonic tumours and normal rat colon.
Osteoarthritis
Changes in synovial fluid N-acetyl-beta-glucosaminidase activity in the human temporomandibular joint with dysfunction.
Periodontal Diseases
[Beta-glucuronidase, beta-acetylgalactosaminidase and acid phosphatase activities in whole saliva of the patients with periodontal disease (author's transl)]
Primary Immunodeficiency Diseases
Therapeutic role of hematopoietic stem cells in autism spectrum disorder-related inflammation.
Sandhoff Disease
Impaired degradation of chondroitin sulfate in GM2-gangliosidosis.
Tay-Sachs Disease
Impaired degradation of chondroitin sulfate in GM2-gangliosidosis.
Uterine Cervical Neoplasms
Histochemical studies on N-acetyl-beta-galactosaminidase and N-acetyl-beta-glucosaminidase in the cancer of the uterine cervix.
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0.42
4-methylumbelliferyl beta-N-acetylgalactosaminide
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0.35 - 1
4-nitrophenyl beta-acetylgalactosaminide
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0.09 - 1.74
4-nitrophenyl beta-N-acetylgalactosaminide
0.35
4-nitrophenyl beta-acetylgalactosaminide
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1
4-nitrophenyl beta-acetylgalactosaminide
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0.09
4-nitrophenyl beta-N-acetylgalactosaminide
mutant E608Q, pH 6.0, 37°C
0.2
4-nitrophenyl beta-N-acetylgalactosaminide
mutant E608D, pH 6.0, 37°C
0.35
4-nitrophenyl beta-N-acetylgalactosaminide
pH 6.0, 37°C
1.74
4-nitrophenyl beta-N-acetylgalactosaminide
mutant D607E, pH 6.0, 37°C
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0.08 - 7.3
4-nitrophenyl beta-N-acetylgalactosaminide
0.08
4-nitrophenyl beta-N-acetylgalactosaminide
mutant E608Q, pH 6.0, 37°C
0.1
4-nitrophenyl beta-N-acetylgalactosaminide
mutant D607E, pH 6.0, 37°C
0.38
4-nitrophenyl beta-N-acetylgalactosaminide
mutant E608D, pH 6.0, 37°C
7.3
4-nitrophenyl beta-N-acetylgalactosaminide
wild-type, pH 6.0, 37°C
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0.06 - 20.9
4-nitrophenyl beta-N-acetylgalactosaminide
0.06
4-nitrophenyl beta-N-acetylgalactosaminide
mutant D607E, pH 6.0, 37°C
0.88
4-nitrophenyl beta-N-acetylgalactosaminide
mutant E608Q, pH 6.0, 37°C
1.9
4-nitrophenyl beta-N-acetylgalactosaminide
mutant E608D, pH 6.0, 37°C
20.9
4-nitrophenyl beta-N-acetylgalactosaminide
pH 6.0, 37°C
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0.0000013
N-acetylgalactosaminide thiazoline
pH 6.0, 37°C
0.0468
N-acetylglucosaminide thiazoline
pH 6.0, 37°C
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0.0028 - 0.0095
methyl 2,5-dideoxy-2,5-imino-D-allonamide
0.00035 - 0.001
methyl 2,5-dideoxy-2,5-imino-D-mannonamide
0.0036 - 0.025
methyl 2,5-dideoxy-2,5-imino-D-talonamide
0.0073 - 0.026
methyl 2,5-dideoxy-2,5-imino-L-gulonamide
0.0028
methyl 2,5-dideoxy-2,5-imino-D-allonamide
Homo sapiens
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pH 4.5, 37°C
0.0095
methyl 2,5-dideoxy-2,5-imino-D-allonamide
Aspergillus oryzae
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pH 4.5, 37°C
0.00035
methyl 2,5-dideoxy-2,5-imino-D-mannonamide
Aspergillus oryzae
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pH 4.5, 37°C
0.001
methyl 2,5-dideoxy-2,5-imino-D-mannonamide
Homo sapiens
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pH 4.5, 37°C
0.0036
methyl 2,5-dideoxy-2,5-imino-D-talonamide
Homo sapiens
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pH 4.5, 37°C
0.025
methyl 2,5-dideoxy-2,5-imino-D-talonamide
Aspergillus oryzae
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pH 4.5, 37°C
0.0073
methyl 2,5-dideoxy-2,5-imino-L-gulonamide
Aspergillus oryzae
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pH 4.5, 37°C
0.026
methyl 2,5-dideoxy-2,5-imino-L-gulonamide
Homo sapiens
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pH 4.5, 37°C
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additional information
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pH 7.2, activity during the two phases of the oestrous cycle, with significant higher levels of activity during the follicular phase
additional information
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assay described, enzyme activity measured in homogenates of 10 to 15 male and female flies before and after sucrose meals, monitoring by spectroscopy, values of relative activity indicated
additional information
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beta-N-acetylgalactosaminidase shows higher activities at the early follicular phase, which decreased after ovulation, pH 7.2
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5.5
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p-nitrophenyl beta-N-acetylgalactosaminide
6.6
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assay at, pH-optimum estimated for
6
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4-methylumbelliferyl beta-N-acetylgalactosaminide
6
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4-methylumbelliferyl beta-N-acetylgalactosaminide
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4
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not active below, p-nitrophenyl beta-N-acetylgalactosaminide
5.5 - 9.5
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enzyme retains more than 80% activity
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4 - 45
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enzyme gradually inactivated in solution at 4°C, reactivated by incubation with DTT
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brenda
AT173-1
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brenda
AT173-1
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brenda
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brenda
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UniProt
brenda
desert and oasis flies studied, male and female, Neot Hakikar (oasis), Jordan Valley spring (wet), Kfar Adumim starved (arid), Jordan Valley autumn (arid)
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brenda
rat
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brenda
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brenda
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brenda
calf
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brenda
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brenda
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brenda
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brenda
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whole fly homogenates
brenda
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brenda
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brenda
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brenda
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brenda
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AT 173-1, culture broth centrifuged to remove cells
brenda
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AT 173-1, culture broth centrifuged to remove cells
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brenda
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brenda
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brenda
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brenda
additional information
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oviducal fluid
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brenda
additional information
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oviductal fluid
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brenda
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E9RH20_9BACL
1034
1
114733
TrEMBL
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115000
x * 115000, SDS-PAGE
48000
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SDS-PAGE, enzyme denatured with SDS and 2-mercaptoethanol
54000
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native enzyme, gel filtration
82000
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sucrose density gradient centrifugation
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D607E
Km is increased by 5-fold, the kcat is decreased to 1.4% of wild-type
D607N
activity is completely abolished
E608D
Km value is decreased to about 57% and the kcat value is decreased to about 5.2% of wild-type
E608Q
Km value is decreased to about 26% and the kcat value is decreased to about 1.1% of wild-type
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cysteine, stabilization
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dithiothreitol, stabilization
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-20 ºC, inactivation after several weeks
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expression in Escherichia coli
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analysis
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comparison of glycolytic and chitinolytic enzyme activities between desert and oasis flies of Phlebotomus papatasi to evaluate potential differences in susceptibility to infection with Leishmania major
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Izumi, T.; Suzuki, K.
Neutral beta-N-acetylhexosaminidases of rat brain. Purification and enzymatic and immunological characterization
J. Biol. Chem.
258
6991-6999
1983
Rattus norvegicus
brenda
Frohwein, Y.Z.; Gatt, S.
Isolation of beta-N-acetylhexosaminidase, beta-N-acetylglucosaminidase, and beta-N-acetylgalactosaminidase from calf brain
Biochemistry
6
2775-2782
1967
Bos taurus
brenda
Tanaka, A.; Ozaki, S.
Purification and characterization of beta-N-acetylgalactosaminidase from Bacillus sp. AT173-1
J. Biochem.
122
330-336
1997
Bacillus sp. (in: Bacteria), Bacillus sp. (in: Bacteria) AT173-1
brenda
Jacobson, R.L.; Studentsky, L.; Schlein, Y.
Glycolytic and chitinolytic activities of Phlebotomus papatasi (Diptera: Psychodidae) from diverse ecological habitats
Folia Parasitol.
54
301-309
2007
Phlebotomus papatasi
brenda
Carrasco, L.C.; Coy, P.; Aviles, M.; Gadea, J.; Romar, R.
Glycosidase determination in bovine oviducal fluid at the follicular and luteal phases of the oestrous cycle
Reprod. Fertil. Dev.
20
808-817
2008
Bos taurus
brenda
Carrasco, L.C.; Romar, R.; Aviles, M.; Gadea, J.; Coy, P.
Determination of glycosidase activity in porcine oviductal fluid at the different phases of the estrous cycle
Reproduction
136
833-842
2008
Sus scrofa domesticus
brenda
Sumida, T.; Fujimoto, K.; Ito, M.
Molecular cloning and catalytic mechanism of a novel glycosphingolipid-degrading beta-N-acetylgalactosaminidase from Paenibacillus sp. TS12
J. Biol. Chem.
286
14065-14072
2011
Paenibacillus sp. (E9RH20)
brenda
Ayers, B.J.; Glawar, A.F.; Martinez, R.F.; Ngo, N.; Liu, Z.; Fleet, G.W.; Butters, T.D.; Nash, R.J.; Yu, C.Y.; Wormald, M.R.; Nakagawa, S.; Adachi, I.; Kato, A.; Jenkinson, S.F.
Nine of 16 stereoisomeric polyhydroxylated proline amides are potent beta-N-acetylhexosaminidase inhibitors
J. Org. Chem.
79
3398-3409
2014
Aspergillus oryzae, Homo sapiens
brenda
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