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1,3-1,4-beta-D-glucan + H2O
?
-
LamR cleaves the 1,4-beta-linkages of 3-O-substituted beta-glucose in beta-glucans such as lichenin and 1,3-1,4-beta-glucan from the cell walls of barley endosperm, analysis of reaction products
-
-
?
1,3-1,4-beta-D-glucan + H2O
cellooligosaccharide
1,3-beta-oligosaccharide + H2O
?
-
from DP3 to DP6
-
-
?
alpha-laminariheptaosyl fluoride
?
-
substrate can make a nucleophilic attack upon itself, the major product being a cyclic beta-1,3-heptaglucan. NMR confirms uniquely beta-1,3-linkages and no reducing end. The cyclic laminariheptaose molecule is not completely planar and torsion angles at the glycosidic linkages fluctuate between two energy minima
-
?
amygdalin + H2O
?
-
slight activity
-
-
?
barley beta-1,3-1,4-D-glucan + H2O
cellotriose
-
-
-
?
barley beta-glucan + H2O
?
beta-1,3-1,4-D-glucotriose + H2O
D-glucose + cellobiose
-
-
-
?
beta-1,3-1,4-glucan + H2O
?
beta-D-glucan
?
source yeast cell wall
-
-
?
beta-D-glucan + H2O
?
source of substrate: barley
-
-
?
beta-Glc-(1->3)-beta-Glc-(1->4)-beta-Glc-(1->3)-beta-Glc-(1->4)-Glc + H2O
beta-Glc-(1->3)-beta-Glc-(1->4)-beta-Glc + ?
-
-
main product, plus some tetrasaccharide. The enzyme acts on both beta-1,4-linkages of substrate
-
?
beta-glucan + H2O
laminaridextrins
birchwood xylan + H2O
?
-
-
-
?
carboxymethyl cellulose + H2O
?
carboxymethyl-cellulose + H2O
?
15% of the activity with beta-D-glucan
-
-
?
carboxymethylcellulose + H2O
?
carboxymethylcellulose + H2O
cellobiose + cellotriose + glucose
carboxymethylcellulose + H2O
D-glucose + ?
-
-
-
?
carboxymethylpachyman + H2O
?
-
-
-
?
carboxymethylpachyman + H2O
D-glucose + carboxymethyl-(1-3,1-4)-beta-D-glucan
carob bean gum + H2O
?
-
-
-
?
cellobiose + H2O
2 D-glucose
-
-
-
?
cellodextrin + H2O
cellobiose + cellotriose + higher oligosaccharides
-
-
-
?
cellohexaose + H2O
?
-
-
-
?
cellopentaose + H2O
?
-
-
-
?
cellopentaose + H2O
cellobiose + cellotriose
cellotetraose + H2O
?
-
-
-
?
cellulose + H2O
?
bacterial crystalline cellulose
-
-
?
cellulose + H2O +
?
-
80% of the activity with laminarin
-
-
?
CM-curdlan + H2O
?
-
80.7% of the activity with CM-curdlan
-
-
?
curdlan + H2O
laminaribiose + laminaritriose + ?
-
main products
-
?
curdlan + H2O
laminaripentaose
curdlan + H2O +
?
-
54.6% of the activity with laminarin
-
-
?
gentiotriose + H2O
?
-
slight activity
-
-
?
Glc-beta(1,4)-Glc-beta(1,3)-Glc-methylumbelliferyl + H2O
methylumbelliferone + Glc-beta(1,4)-Glc-beta(1,3)-Glc
-
-
-
-
?
konjac glucomannan + H2O
?
40% of the activity with barley beta-1,3-1,4-D-glucan
-
-
?
laminaribiose + H2O
2 D-glucose
-
39% of the activity with laminarin
-
-
?
laminaribiose + H2O
D-glucose
laminariheptaose + H2O
?
-
-
-
?
laminarihexaose + H2O
D-glucose + laminaribiose + laminaritriose
-
-
-
?
laminarin + H2O
6-O-glucosyl-laminaritriose + ?
6-O-glucosyl-laminaritriose is produced as one of the major products
-
-
?
laminarin + H2O
D-glucose + ?
-
-
-
-
?
laminarin + H2O
D-glucose + laminaribiose + laminaritriose + laminaritetraose + laminaripentaose + laminarihexaose
laminarin + H2O
D-glucose + laminarioligosaccharides
laminarin + H2O
D-glucose + laminaripentaose
-
from Laminaria digitata, best substrate for LIC 1, cleaves internal bonds, LIC 1 may have five subsites for glucose at the aglycone site and at least one subsite in the glucone site, LIC 1 should move along the substrate 1 or 5 glucosyl residues after each hydrolytic step, after the hydrolytic step LIC 1 dissociates from or moves along the substrate molecule to the next beta-1,3-linkage distant one or preferentially 4-6 glucosyl residues in the non-reducing end direction
LIC 1 forms almost only glucose and laminaripentaose as soluble products, laminaripentaose is the main product, glucose is 20% from total products and 25% from soluble products
-
?
laminarin + H2O
laminaribiose + D-glucose
-
-
-
-
?
laminarin + H2O
laminaritriose
from Laminaria digitata
-
-
?
laminaripentaose + H2O
D-glucose + laminaribiose + laminaritriose
-
-
-
?
laminaritetraose + 2 H2O
laminaribiose + 2 D-glucose
laminaritetraose + H2O
2 laminaribiose
-
-
-
?
laminaritetraose + H2O
?
-
-
-
-
?
laminaritetraose + H2O
D-glucose + laminaribiose + laminaritriose
laminaritriose + H2O
D-glucose + laminaribiose
laminaritriose + H2O
laminaribiose + D-glucose
-
-
-
-
?
lichenan + H2O
4-O-glucosyl-laminaribiose + ?
4-O-glucosyl-laminaribiose is produced as one of the major products
-
-
?
lichenan + H2O
beta-D-glucopyranosyl-(1-3)-beta-D-glucopyranosyl-(1-4)-D-glucopyranose + ?
-
-
-
-
?
lichenan + H2O
beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->3)-beta-D-glucopyranose + ?
-
from Cetraria islandica. HdLam33 produces no cellotriose, cellobiose, and glucose from lichenan, but produced the trisaccharide cellobiosyl-beta-1,3-glucose
-
-
?
lichenan + H2O
cellooligosaccharide
lichenin + H2O
?
-
LamR cleaves the 1,4-beta-linkages of 3-O-substituted beta-glucose in beta-glucans such as lichenin, analysis of reaction products
-
-
?
lichenin + H2O
beta-D-glucopyranosyl-(1->3)-[beta-D-glucopyranosyl-(1->3)-beta-D-glucopyranosyl-(1->4)]-beta-D-glucopyranose + lichenin oligomers
lichenin + H2O
cellotetraose + cellopentaose + glucose
-
from Cetraria islandica, cleaves internal bonds, LIC 1 has high affinity for sequences of cellotetraose linked by beta-1,3-links releasing cellotetraose from lichenin, after the hydrolytic step LIC 1 dissociates from or moves along the substrate molecule to the next beta-1,3-linkage distant one or preferentially 4-6 glucosyl residues in the non-reducing end direction
main products are cellotetraose and cellopentaose, glucose is only 1% of soluble products, LIC 1 produces an additional saccharide as product possibly containing beta-1,3- and beta-1,4-linkages
-
?
oxidized laminarin
laminaritetraose + laminaritriose + laminaribiose + D-glucose
-
-
-
?
p-nitrophenyl-beta-D-glucopyranoside + H2O
D-glucose + p-nitrophenol
-
slight activity
-
?
pachyman + H2O
laminarisaccharides
periodate-oxidized laminarin + H2O
?
-
-
-
?
polysaccharides + H2O
oligosaccharides
-
role in maintenance of channels for communication and translocation through cell walls
-
?
short-chain pachyman + H2O
?
-
-
-
-
?
zymosan A + H2O
?
from Saccharomyces cerevisiae
-
-
?
additional information
?
-
1,3-1,4-beta-D-glucan + H2O
cellooligosaccharide
-
soluble, from barley, endo-acting enzyme, preference for mixed-link 1,3-1,4-beta-D-glucan substrates, highest catalytic efficiency with barley 1,3-1,4-beta-D-glucan
spectrum of oligosaccharide products by EG VI and EG VII including linear beta-1,4-gluco and mixed-link oligosaccharides, relative distribution of the cellooligosaccharides DP 1-5 after 24 h incubation
-
?
1,3-1,4-beta-D-glucan + H2O
cellooligosaccharide
-
soluble, from barley, endo-acting enzyme, preference for mixed-link 1,3-1,4-beta-D-glucan substrates, highest catalytic efficiency with barley 1,3-1,4-beta-D-glucan
spectrum of oligosaccharide products by EG VI and EG VII including linear beta-1,4-gluco and mixed-link oligosaccharides, relative distribution of the cellooligosaccharides DP 1-5 after 24 h incubation
-
?
avicel + H2O
?
-
-
-
?
avicel + H2O
?
-
86% of the activity with laminarin
-
-
?
barley beta-glucan + H2O
?
-
-
-
?
barley beta-glucan + H2O
?
-
-
-
?
barley beta-glucan + H2O
?
-
-
-
?
barley beta-glucan + H2O
?
-
-
-
?
barley beta-glucan + H2O
?
-
-
-
?
barley beta-glucan + H2O
?
-
-
-
-
?
barley beta-glucan + H2O
?
-
-
-
?
barley beta-glucan + H2O
?
-
-
-
-
?
barley beta-glucan + H2O
?
-
-
-
?
barley beta-glucan + H2O
?
-
-
-
-
?
beta-1,3-1,4-glucan + H2O
?
-
from Hordeum vulgare
-
-
?
beta-1,3-1,4-glucan + H2O
?
from Hordeum vulgare, preferred substrate
-
-
?
beta-1,3-1,4-glucan + H2O
?
from Hordeum vulgare, preferred substrate
-
-
?
beta-1,3-1,4-glucan + H2O
?
-
-
high viscosity glucan, 41% of the activity with laminarin, medium and low viscosity, 9% of the activity with laminarin, respectively
-
?
beta-D-glucans + H2O
?
-
-
-
-
?
beta-D-glucans + H2O
?
-
enzyme involved in cell wall metabolism
-
-
?
beta-D-glucans + H2O
?
-
role in budding process and cell wall growth
-
-
?
beta-D-glucans + H2O
?
-
in vivo probably facilitation of solubilisation of cell wall glucan
-
-
?
beta-D-glucans + H2O
?
-
-
-
-
?
beta-glucan + H2O
?
-
-
-
-
?
beta-glucan + H2O
?
-
-
-
?
beta-glucan + H2O
?
-
barley beta-glucan
-
-
?
beta-glucan + H2O
?
-
barley beta-glucan
-
-
?
beta-glucan + H2O
?
-
-
-
-
?
beta-glucan + H2O
?
-
-
-
-
?
beta-glucan + H2O
?
-
barley beta-glucan
-
-
?
beta-glucan + H2O
?
-
-
-
-
?
beta-glucan + H2O
?
the enzyme hydrolyses the beta-1,4-glycosidic linkage of barley beta-glucan through an inverting mechanism
-
-
?
beta-glucan + H2O
?
-
degree of hydrolysis reaches 10%, products are oligosaccharides with degree of polymerization 2-10
-
-
?
beta-glucan + H2O
laminaridextrins
-
-
-
?
beta-glucan + H2O
laminaridextrins
-
yeast cell-wall 1,3-beta-glucan
-
-
?
beta-glucan + H2O
laminaridextrins
Flavobacterium dormitator
-
yeast glucan
-
?
beta-glucan + H2O
laminaridextrins
Flavobacterium dormitator
-
yeast glucan
-
-
?
beta-glucan + H2O
laminaridextrins
-
-
D-glucose + cellobiose
?
beta-glucan + H2O
laminaridextrins
-
1-4)-beta-glucan
-
?
beta-glucan + H2O
laminaridextrins
-
beta-(1-3)-beta-(1-4)mixed-linked, from barley
-
?
beta-glucan + H2O
laminaridextrins
-
-
-
?
beta-glucan + H2O
laminaridextrins
-
-
-
?
beta-glucan + H2O
laminaridextrins
-
beta-(1-3)-beta-(1-4)mixed-linked, from barley
-
?
beta-glucan + H2O
laminaridextrins
-
beta-(1-3)-beta-(1-4)mixed-linked, from barley
-
?
carboxymethyl cellulose + H2O
?
-
-
-
?
carboxymethyl cellulose + H2O
?
-
-
-
?
carboxymethyl cellulose + H2O
?
-
49.6% of the activity with laminarin
-
-
?
carboxymethylcellulose + H2O
?
45% of the activity with barley beta-1,3-1,4-D-glucan
-
-
?
carboxymethylcellulose + H2O
?
45% of the activity with barley beta-1,3-1,4-D-glucan
-
-
?
carboxymethylcellulose + H2O
cellobiose + cellotriose + glucose
-
soluble, 1,4-beta-D-glucan, endo-acting enzyme
EG VI releases mainly cellobiose and almost equimolar amounts of cellotriose and glucose, EG VII yields mainly cellobiose and glucose as final reaction products
-
?
carboxymethylcellulose + H2O
cellobiose + cellotriose + glucose
-
soluble, 1,4-beta-D-glucan, endo-acting enzyme
EG VI releases mainly cellobiose and almost equimolar amounts of cellotriose and glucose, EG VII yields mainly cellobiose and glucose as final reaction products
-
?
carboxymethylpachyman + H2O
D-glucose + carboxymethyl-(1-3,1-4)-beta-D-glucan
-
-
-
?
carboxymethylpachyman + H2O
D-glucose + carboxymethyl-(1-3,1-4)-beta-D-glucan
-
-
-
?
carboxymethylpachyman + H2O
D-glucose + carboxymethyl-(1-3,1-4)-beta-D-glucan
-
-
-
?
cellopentaose + H2O
cellobiose + cellotriose
-
-
-
?
cellopentaose + H2O
cellobiose + cellotriose
-
-
-
?
chitosan + H2O
?
-
-
-
?
curdlan + H2O
?
from Alcaligenes faecalis
-
-
?
curdlan + H2O
?
-
from Alcaligenes faecalis, LamR cleaves the 1,3-beta-linkages of 3-O-substituted glucose units in 1,3-beta-glucans such as laminarin and curdlan
-
-
?
curdlan + H2O
?
-
-
-
-
?
curdlan + H2O
laminaripentaose
Flavobacterium dormitator
-
-
-
-
?
curdlan + H2O
laminaripentaose
Flavobacterium dormitator
-
paramylon
main product
?
laminaran + H2O
?
-
from Laminaria digitata, 1,3-beta-glucan
-
-
?
laminaran + H2O
?
-
from Laminaria digitata, 1,3-beta-glucan
-
-
?
laminaribiose + H2O
D-glucose
-
-
-
?
laminaribiose + H2O
D-glucose
-
-
-
?
laminaribiose + H2O
D-glucose
-
0.03% of the rate of laminarin
-
?
laminarin + H2O
?
6.2% of the activity with beta-D-glucan
-
-
?
laminarin + H2O
?
-
AkLam36 exhibits exolytic beta-1,3-hydrolytic activity releasing D-glucose preferring beta-1,3-glucans with a few beta-1,6-linked glucose branches such as Laminaria digitata laminarin
-
-
?
laminarin + H2O
?
-
AkLam36 exhibits exolytic beta-1,3-hydrolytic activity with released D-glucose, preferring beta-1,3-glucans with a few beta-1,6-linked glucose branches such as Laminaria digitata laminarin, best substrate is barley beta-D-glucan
-
-
?
laminarin + H2O
?
-
-
-
?
laminarin + H2O
?
-
-
-
?
laminarin + H2O
?
Flavobacterium dormitator
-
use as carbon source
-
-
?
laminarin + H2O
?
-
-
-
?
laminarin + H2O
?
-
-
-
?
laminarin + H2O
?
48% of the activity with curdlan
-
-
?
laminarin + H2O
?
48% of the activity with curdlan
-
-
?
laminarin + H2O
?
-
-
-
-
?
laminarin + H2O
?
-
-
-
?
laminarin + H2O
?
-
-
-
?
laminarin + H2O
?
-
-
-
?
laminarin + H2O
?
-
33.2% of the activity with CM-curdlan
-
-
?
laminarin + H2O
?
-
from Laminaria digitata, LamR cleaves the 1,3-beta-linkages of 3-O-substituted glucose units in 1,3-beta-glucans such as laminarin and curdlan, analysis of reaction products
-
-
?
laminarin + H2O
?
-
-
-
-
?
laminarin + H2O
?
-
-
-
-
?
laminarin + H2O
?
-
-
-
-
?
laminarin + H2O
D-glucose + laminaribiose + laminaritriose + laminaritetraose + laminaripentaose + laminarihexaose
-
-
reducing sugars
?
laminarin + H2O
D-glucose + laminaribiose + laminaritriose + laminaritetraose + laminaripentaose + laminarihexaose
-
-
-
?
laminarin + H2O
D-glucose + laminaribiose + laminaritriose + laminaritetraose + laminaripentaose + laminarihexaose
-
-
70-90% D-glucose, small amounts of laminaribiose and D-glucose oligomers of 3-4 D-glucose units
?
laminarin + H2O
D-glucose + laminaribiose + laminaritriose + laminaritetraose + laminaripentaose + laminarihexaose
-
-
laminaridextrins + D-glucose
?
laminarin + H2O
D-glucose + laminaribiose + laminaritriose + laminaritetraose + laminaripentaose + laminarihexaose
Flavobacterium dormitator
-
-
main product laminaripentaose
?
laminarin + H2O
D-glucose + laminaribiose + laminaritriose + laminaritetraose + laminaripentaose + laminarihexaose
Flavobacterium dormitator
-
-
D-glucose + laminaribiose, laminaritriose + D-glucose
?
laminarin + H2O
D-glucose + laminaribiose + laminaritriose + laminaritetraose + laminaripentaose + laminarihexaose
-
-
-
?
laminarin + H2O
D-glucose + laminaribiose + laminaritriose + laminaritetraose + laminaripentaose + laminarihexaose
-
-
laminaridextrins + D-glucose
?
laminarin + H2O
D-glucose + laminaribiose + laminaritriose + laminaritetraose + laminaripentaose + laminarihexaose
-
-
reducing sugars
?
laminarin + H2O
D-glucose + laminaribiose + laminaritriose + laminaritetraose + laminaripentaose + laminarihexaose
-
-
-
?
laminarin + H2O
D-glucose + laminaribiose + laminaritriose + laminaritetraose + laminaripentaose + laminarihexaose
-
-
laminaridextrins + D-glucose
?
laminarin + H2O
D-glucose + laminaribiose + laminaritriose + laminaritetraose + laminaripentaose + laminarihexaose
-
-
laminaridextrins + D-glucose
?
laminarin + H2O
D-glucose + laminaribiose + laminaritriose + laminaritetraose + laminaripentaose + laminarihexaose
-
-
D-glucose + laminaribiose
?
laminarin + H2O
D-glucose + laminaribiose + laminaritriose + laminaritetraose + laminaripentaose + laminarihexaose
-
-
laminaridextrins + D-glucose
?
laminarin + H2O
D-glucose + laminaribiose + laminaritriose + laminaritetraose + laminaripentaose + laminarihexaose
-
-
-
?
laminarin + H2O
D-glucose + laminaribiose + laminaritriose + laminaritetraose + laminaripentaose + laminarihexaose
-
-
reducing sugars
?
laminarin + H2O
D-glucose + laminaribiose + laminaritriose + laminaritetraose + laminaripentaose + laminarihexaose
-
-
laminaritriose
?
laminarin + H2O
D-glucose + laminaribiose + laminaritriose + laminaritetraose + laminaripentaose + laminarihexaose
-
-
laminaridextrins + D-glucose
?
laminarin + H2O
D-glucose + laminarioligosaccharides
-
-
-
-
?
laminarin + H2O
D-glucose + laminarioligosaccharides
-
-
-
-
?
laminaritetraose + 2 H2O
laminaribiose + 2 D-glucose
-
-
-
-
?
laminaritetraose + 2 H2O
laminaribiose + 2 D-glucose
-
from Laminaria digitata. HdLam33 performs a transglycosylation reaction transferring the laminaribiose unit in the non-reducing terminus of laminaritriose , as donor substrate, to a free laminaribiose, as acceptor substrate, giving laminaritetraose and glucose. The resulting laminaritetraose is subsequently hydrolyzed by HdLam33 into 2 mol of glucose and 1 mol of laminaribiose
-
-
?
laminaritetraose + H2O
D-glucose + laminaribiose + laminaritriose
-
-
-
?
laminaritetraose + H2O
D-glucose + laminaribiose + laminaritriose
-
-
-
?
laminaritriose + H2O
?
-
-
-
-
?
laminaritriose + H2O
?
-
-
-
-
?
laminaritriose + H2O
D-glucose + laminaribiose
-
-
-
?
laminaritriose + H2O
D-glucose + laminaribiose
-
-
-
?
lichenan + H2O
?
109% of the activity with beta-D-glucan
-
-
?
lichenan + H2O
?
-
-
-
-
?
lichenan + H2O
?
-
-
-
-
?
lichenan + H2O
?
48% of the activity with barley beta-1,3-1,4-D-glucan
-
-
?
lichenan + H2O
?
48% of the activity with barley beta-1,3-1,4-D-glucan
-
-
?
lichenan + H2O
?
-
-
-
-
?
lichenan + H2O
?
-
-
-
-
?
lichenan + H2O
?
-
-
-
-
?
lichenan + H2O
?
-
67.27% of the activity with CM-curdlan
-
-
?
lichenan + H2O
?
-
-
-
-
?
lichenan + H2O
?
-
-
-
-
?
lichenan + H2O
cellooligosaccharide
-
from Cetraria islandica, endo-acting enzyme, preference for mixed-link 1,3-1,4-beta-D-glucan substrates, rapid hydrolysis
spectrum of oligosaccharide products by EG VI and EG VII
-
?
lichenan + H2O
cellooligosaccharide
-
from Cetraria islandica, endo-acting enzyme, preference for mixed-link 1,3-1,4-beta-D-glucan substrates, rapid hydrolysis
spectrum of oligosaccharide products by EG VI and EG VII
-
?
lichenin + H2O
beta-D-glucopyranosyl-(1->3)-[beta-D-glucopyranosyl-(1->3)-beta-D-glucopyranosyl-(1->4)]-beta-D-glucopyranose + lichenin oligomers
-
-
laminaripentaose + D-glucose
?
lichenin + H2O
beta-D-glucopyranosyl-(1->3)-[beta-D-glucopyranosyl-(1->3)-beta-D-glucopyranosyl-(1->4)]-beta-D-glucopyranose + lichenin oligomers
-
-
laminaripentaose + D-glucose
?
lichenin + H2O
beta-D-glucopyranosyl-(1->3)-[beta-D-glucopyranosyl-(1->3)-beta-D-glucopyranosyl-(1->4)]-beta-D-glucopyranose + lichenin oligomers
Flavobacterium dormitator
-
-
laminaripentaose main product
?
lichenin + H2O
beta-D-glucopyranosyl-(1->3)-[beta-D-glucopyranosyl-(1->3)-beta-D-glucopyranosyl-(1->4)]-beta-D-glucopyranose + lichenin oligomers
Flavobacterium dormitator
-
weak activity of enzyme I
-
?
lichenin + H2O
beta-D-glucopyranosyl-(1->3)-[beta-D-glucopyranosyl-(1->3)-beta-D-glucopyranosyl-(1->4)]-beta-D-glucopyranose + lichenin oligomers
-
-
-
?
lichenin + H2O
beta-D-glucopyranosyl-(1->3)-[beta-D-glucopyranosyl-(1->3)-beta-D-glucopyranosyl-(1->4)]-beta-D-glucopyranose + lichenin oligomers
-
-
-
?
lichenin + H2O
beta-D-glucopyranosyl-(1->3)-[beta-D-glucopyranosyl-(1->3)-beta-D-glucopyranosyl-(1->4)]-beta-D-glucopyranose + lichenin oligomers
-
-
-
?
pachyman + H2O
?
223% of the activity with curdlan
-
-
?
pachyman + H2O
?
223% of the activity with curdlan
-
-
?
pachyman + H2O
laminarisaccharides
Flavobacterium dormitator
-
-
-
?
pachyman + H2O
laminarisaccharides
Flavobacterium dormitator
-
weak activity of enzyme I
-
-
?
pachyman + H2O
laminarisaccharides
-
-
-
?
pachyman + H2O
laminarisaccharides
-
more active on short-chain pachyman
-
?
pustulan + H2O
?
-
-
-
?
pustulan + H2O
?
-
slight activity
-
-
?
additional information
?
-
determination of binding constants and binding capacity for substrates tested, in presence and absence of Ca2+
-
-
?
additional information
?
-
-
no direct degradation activity with laminaribiose as a sole substrate, but the enzyme is capable of degrading it via transglycosylation reaction with laminaritriose
-
-
?
additional information
?
-
-
sAkLam36 substrate specificity amd mode of action, overview. The enzyme is inactive toward laminaribiose, starch, carboxymethylcellulose, agar, beta-1,4-mannan, beta-1,4-xylan, and alginic acid, and it is specific to beta-1,3-glucosyl linkages
-
-
?
additional information
?
-
no activity on cellulose
-
-
?
additional information
?
-
-
no activity on cellulose
-
-
?
additional information
?
-
the enzyme acts as a bi-functional enzyme with single catalytic domain
-
-
?
additional information
?
-
enzyme hydrolyzes both beta-1,3-glucan (laminarin) and beta-1,3-1,4-glucan linkages, catalyzes endo-hydrolysis of the beta-1,4 glycosidic linkage adjacent to a 3-O-substituted glucosyl residues in the mixed linked beta-glucans, as well as the beta-1,3 linkage. No substrates: carboxymethylcellulose, avicel
-
-
?
additional information
?
-
activity in decreasing order is barley beta-1,3-1,4-D-glucan, carboxymethylcellulose, lichenan, avicel, konjac glucomannan. No substrates: cellobiose, cellotriose, p-nitrophenyl derivatives of mono- and disaccharides, or beta-1,3-D-glucan, beta-1,4-D-galactomannan, and beta-1,4-D-xylan
-
-
?
additional information
?
-
activity in decreasing order is barley beta-1,3-1,4-D-glucan, carboxymethylcellulose, lichenan, avicel, konjac glucomannan. No substrates: cellobiose, cellotriose, p-nitrophenyl derivatives of mono- and disaccharides, or beta-1,3-D-glucan, beta-1,4-D-galactomannan, and beta-1,4-D-xylan
-
-
?
additional information
?
-
-
laminarinase hydrolyses both beta-1,3-glycosidic bonds and beta-1,4-glycosidic bonds
-
-
?
additional information
?
-
-
laminarinase hydrolyses both beta-1,3-glycosidic bonds and beta-1,4-glycosidic bonds
-
-
?
additional information
?
-
enzyme only acts on substrates containing beta-1,3 glycosidic bonds but not on substrates containing only beta-1,4- or beta-1,6-glycosidic bonds
-
-
?
additional information
?
-
Flavobacterium dormitator
-
transglycosylase activity
-
-
?
additional information
?
-
-
HdLam33 degrades laminarin and laminarioligosaccharides to laminaribiose and glucose
-
-
?
additional information
?
-
no substrates: carboxymethylcellulose, birchwood xylan, avicel and filter paper
-
-
?
additional information
?
-
no substrates: carboxymethylcellulose, birchwood xylan, avicel and filter paper
-
-
?
additional information
?
-
no substrates: xylan, colloidal chitin, carboxymethyl cellulose, polygalacturonic acid, pectin and laminaribiose
-
-
?
additional information
?
-
no substrates: xylan, colloidal chitin, carboxymethyl cellulose, polygalacturonic acid, pectin and laminaribiose
-
-
?
additional information
?
-
-
the ten active-site residues ASn26,Glu63, Arg65, Phe92, Tyr94, Glu105, Asp107, Glu109, Asn182 and Trp184 form a network of hydrogen bonds and hydrophobic stacking interactions with the substrate
-
-
?
additional information
?
-
the enzyme has a broad substrate specificity and hydrolyzes barley-beta-D-glucan before chitosan, carboxymethyl-cellulose, and lichenan
-
-
?
additional information
?
-
the enzyme hydrolyzes cello-oligosaccharides (G3-G6) to cellotriose and cellobiose but not to D-glucose. Barley beta-glucan and lichenan are hydrolyzed by the enzyme to cellotetraose as the major product
-
-
?
additional information
?
-
no activity with carboxymethylcellulose and xylan of all truncated proteins of LamA
-
-
?
additional information
?
-
-
LIC 1 lyses plant and fungal cell walls, making available their contents, LIC 1 may help the cellulases CEL 1 and CEL 2 by hydrolyzing beta-1,3-1,4-glucans, important chiefly in cereal walls
-
-
?
additional information
?
-
-
a group in LIC 1 with pK of 4.9 is necessary for substrate binding
-
-
?
additional information
?
-
the enzyme strictly recognizes beta-D-Glcp-(1,3)-D-Glcp at subsites -2 and -1. It permits 6-O-glucosyl substitution at subsite +1 and a beta-1,4-glucosidic linkage at the catalytic site
-
-
?
additional information
?
-
-
the enzyme strictly recognizes beta-D-Glcp-(1,3)-D-Glcp at subsites -2 and -1. It permits 6-O-glucosyl substitution at subsite +1 and a beta-1,4-glucosidic linkage at the catalytic site
-
-
?
additional information
?
-
-
one active site per enzyme molecule
-
-
?
additional information
?
-
the enzyme shows broad substrate specificity and exhibits high activity on substrates containing beta-1,4-glycosidic bonds and beta-1,3-glycosidic bonds. Although TeEgl5A belongs to endo-1,4-glucanase, it also acts on the 1,3-beta-linkage or mixed-linkage-beta-glucan. The enzyme has a higher specific activity on soluble 1,3-1,4-beta-glucans and 1,4-beta-glucans, such as beta-glucan (100%), laminarin (95%), and lichenin (80%), than on less soluble polysaccharides like carboxymethyl cellulose (15%). No activity on Avicel, cellobiose, and cellotriose. The broader substrate specificity suggests that TeEgl5A belongs to the group of nonspecific endoglucanases
-
-
?
additional information
?
-
-
the enzyme shows broad substrate specificity and exhibits high activity on substrates containing beta-1,4-glycosidic bonds and beta-1,3-glycosidic bonds. Although TeEgl5A belongs to endo-1,4-glucanase, it also acts on the 1,3-beta-linkage or mixed-linkage-beta-glucan. The enzyme has a higher specific activity on soluble 1,3-1,4-beta-glucans and 1,4-beta-glucans, such as beta-glucan (100%), laminarin (95%), and lichenin (80%), than on less soluble polysaccharides like carboxymethyl cellulose (15%). No activity on Avicel, cellobiose, and cellotriose. The broader substrate specificity suggests that TeEgl5A belongs to the group of nonspecific endoglucanases
-
-
?
additional information
?
-
-
enzyme is active on 1,3-1,4-beta-D-glucans, with no activity against a range of aryl and methylumbelliferyl-beta-glycosides, alpha- and beta-linked polysaccharides, including oat spelts xylan, rhodymenan, and polygalacturonic acid, avicel, laminarin or pachyman
-
-
?
additional information
?
-
-
enzyme is active on 1,3-1,4-beta-D-glucans, with no activity against a range of aryl and methylumbelliferyl-beta-glycosides, alpha- and beta-linked polysaccharides, including oat spelts xylan, rhodymenan, and polygalacturonic acid, avicel, laminarin or pachyman
-
-
?
additional information
?
-
-
one active site per enzyme molecule
-
-
?
additional information
?
-
the enzyme shows broad substrate specificity and exhibits high activity on substrates containing beta-1,4-glycosidic bonds and beta-1,3-glycosidic bonds. Although TeEgl5A belongs to endo-1,4-glucanase, it also acts on the 1,3-beta-linkage or mixed-linkage-beta-glucan. The enzyme has a higher specific activity on soluble 1,3-1,4-beta-glucans and 1,4-beta-glucans, such as beta-glucan (100%), laminarin (95%), and lichenin (80%), than on less soluble polysaccharides like carboxymethyl cellulose (15%). No activity on Avicel, cellobiose, and cellotriose. The broader substrate specificity suggests that TeEgl5A belongs to the group of nonspecific endoglucanases
-
-
?
additional information
?
-
-
transglycosylase activity
-
-
?
additional information
?
-
-
hydrolysis occurs with retention of the beta-configuration at the anomeric carbon, LamR performs transglycosylation to generate both 1,3-beta-glycosidic and 1,4-beta-glycosidic linkages, a laminaribiosyl unit is the minimum requirement for formation of an enzyme-substrate complex and subsequent hydrolysis/transglycosylation, no substrate: laminaribiose
-
-
?
additional information
?
-
-
the C-terminal region of isoform Eng1 acts as carbohydrate-binding module. In vitro, the purified module has affinity for beta-(1-3)-glucan chains with a minimum degree of polymerization of 30 glucose units
-
-
?
additional information
?
-
SsGlc functions in salt stress tolerance in Synechocystis PCC6803
-
-
?
additional information
?
-
-
SsGlc functions in salt stress tolerance in Synechocystis PCC6803
-
-
?
additional information
?
-
no activity towards cellulose, curdlan or laminarin
-
-
?
additional information
?
-
-
no activity towards cellulose, curdlan or laminarin
-
-
?
additional information
?
-
-
no substrates: xylan, microcrystalline cellulose, and 4-nitrophenylbeta-D-glucopyranoside
-
-
?
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Schwarz, W.; Bronnenmeier, K.; Staudenbauer, W.L.
Molecular cloning of Clostridium thermocellum genes involved in beta-glucan degradation in bacteriophage lambda
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7
859-864
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Acetivibrio thermocellus
-
brenda
Manners, D.J.; Wilson, G.
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1973
Flavobacterium sp.
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Paenibacillus macerans
-
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Preliminary characterization of laminarinase from Trichoderma longibrachiatum
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Trichoderma longibrachiatum
-
brenda
Totsuka, A.; Usui, T.
Separation and characterization of the endo-beta-(1-3)-D-glucanase from Rhizoctonia solani
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50
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Rhizoctonia solani
-
brenda
Leatham, G.F.
Extracellular enzymes produced by the cultivated mushroom Lentinus edodes during degradation of a lignocellulosic medium
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50
859-867
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Lentinula edodes
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Wong, Y.S.; MacClachlan, G.A.
1,3-beta-D-Glucanases from Pisum sativum seedlings
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65
222-228
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Pisum sativum
brenda
Villa, T.G.; Phaff, H.J.
Recovery of invertase and laminarinases from industrial waste broth of baker's yeast
Eur. J. Appl. Microbiol. Biotechnol.
9
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1980
Saccharomyces cerevisiae
-
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Martinez, M.J.; Reyes, F.; Lahoz, R.; Perez-Leblic, M.I.
Lytic enzymes in autolysis of Botrytis cinerea
FEMS Microbiol. Lett.
19
157-160
1983
Botrytis cinerea
-
brenda
Nagasaki, S.; Mori, H.; Yamamoto, S.
Characterization of endo beta-1,3-glucanase IV from Flavobacterium dorminator var. glucanolyticae FA-5
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45
2689-2694
1981
Flavobacterium dormitator
-
brenda
Kawai, M.; Noguchi, M.; Shimura, G.; Suga, Y.; Samejima, H.
Effect of different culture conditions on production of cellulolytic and plant tissue macerating enzymes by Irpex lacteus Fr.
Agric. Biol. Chem.
42
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1978
Irpex lacteus
-
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Salyers, A.A.; Palmer, J.K.; Wilkins, T.D.
Laminarinase (beta-glucanase) activity in Bacteroides from the human colon
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33
1118-1124
1977
Bacteroides fragilis, Bacteroides thetaiotaomicron, Parabacteroides distasonis
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Production and catabolite repression of Penicillium italicum beta-glucanases
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129
52-58
1977
Penicillium italicum
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Barley (1-3,1-4)-beta-glucanase isoenzyme E1 gene expression is medaited by auxin and giberellic acid
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306
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Hordeum vulgare
brenda
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37
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1992
Bacillus licheniformis
-
brenda
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A study of the action of laminarinases from Spisula sacchalinensis by the use of nuclear magnetic resonance
Biochim. Biophys. Acta
268
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1972
Pseudocardium sachalinense
brenda
Tingle, M.A.; Halvorson, H.O.
A comparison of beta-glucanase and beta-glucosidase in Saccharomyces lactis
Biochim. Biophys. Acta
250
165-171
1971
Kluyveromyces lactis
brenda
Sova, V.V.; Elyakova, L.A.; Vaskovsky, V.E.
Purification and some properties of beta-1,3-glucan glucanohydrolase from the crystalline style of bivalvia, Spisula sacchalinensis
Biochim. Biophys. Acta
212
111-115
1970
Pseudocardium sachalinense
brenda
Totani, K.; Harumiya, S.; Nanjo, F.; Usui, T.
Substrate affinity chromatography of beta-1,3-glucanase from Basidiomycetes species
Agric. Biol. Chem.
47
1159-1162
1983
Basidiomycota
-
brenda
Villa, T.G.; Notario, V.; Villanueva, J.R.
Occurrence of an endo-1,3-beta-glucanase in culture fluids of the yeast Candida utilis. Purification and characterization of the enzyme activity
Biochem. J.
177
107-114
1979
Cyberlindnera jadinii
brenda
Manners, D.J.; Marshall, J.J.
Some properties of a beta-1,3-glucanase from rye
Phytochemistry
12
547-553
1973
Secale cereale
-
brenda
Mueller, J.J.; Thomsen, K.K.; Heinemann, U.
Crystal structure of barley 1,3-1,4-beta-glucanase at 2.0-A resolution and comparison with Bacillus 1,3-1,4-beta-glucanase
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273
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1998
Hordeum vulgare
brenda
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Purification and characterization of nonlytic endo-beta-1,3-glucanase I from Flavobacterium dormitator var. glucanolyticae
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54
2107-2114
1990
Flavobacterium dormitator
-
brenda
Zverlov, V.V.; Volkov, I.Y.; Velikodvorskaya, T.V.; Schwarz, W.H.
Highly thermostable endo-1,3-beta-glucanase (laminarinase) LamA from Thermotoga neapolitana: nucleotide sequence of the gen and characterization of the recombinant gene product
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143
1701-1708
1997
Thermotoga neapolitana
brenda
Ekinci, M.S.; McCrae, S.I.; Flint, H.J.
Isolation and overexpression of a gene encoding an extracellular beta-(1,3-1,4)-glucanase from Streptococcus bovis JB1
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63
3752-3756
1997
Streptococcus equinus JB1
brenda
Nagata, S.; Isafumi, M.; Ishihara, F.; Misono, H.; Nagasaki, S.
Cloning and expression of endo-beta-1,3-glucanase gene from Flavobacterium dorminator in Escherichia coli and characterization of the gene product
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54
2675-2680
1990
Flavobacterium dormitator
brenda
Bang, M.L.; Villadsen, I.; Sandal, T.
Cloning and characterization of an endo-beta-1,3(4)glucanase and an aspartic protease from Phaffia rhodozyma CBS 6938
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51
215-222
1999
Phaffia rhodozyma, Phaffia rhodozyma CBS 6938
brenda
Thrane, C.; Tronsmo, A.; Jensen, D.F.
endo-1,3-beta-Glucanase and cellulase from Trichoderma harzianum: purification and partial characterization, induction of and biological activity against plant pathogenic Pythium spp.
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103
331-344
1997
Trichoderma harzianum
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Petersen, B.O.; Krah, M.; Duus, J.O.; Thomsen, K.K.
A transglycosylating 1,3(4)-beta-glucanase from Rhodothermus marinus. NMR analysis of enzyme reactions
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267
361-369
2000
Rhodothermus marinus
brenda
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Action pattern, specificity, lytic activities, and physiological role of five digestive beta-glucanases isolated from Periplaneta americana
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33
1085-1097
2003
Periplaneta americana
brenda
McCarthy, T.; Hanniffy, O.; Savage, A.V.; Tuohy, M.G.
Catalytic properties and mode of action of three endo-beta-glucanases from Talaromyces emersonii on soluble beta-1,4- and beta-1,3;1,4-linked glucans
Int. J. Biol. Macromol.
33
141-148
2003
Rasamsonia emersonii, Rasamsonia emersonii CBS 814.70
brenda
Zhao, G.; Ali, E.; Araki, R.; Sakka, M.; Kimura, T.; Sakka, K.
Function of the family-9 and family-22 carbohydrate-binding modules in a modular beta-1,3-1,4-glucanase/xylanase derived from Clostridium stercorarium Xyn10B
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69
1562-1567
2005
Thermoclostridium stercorarium
brenda
Kim, K.H.; Kim, Y.O.; Ko, B.S.; Youn, H.J.; Lee, D.S.
Over-expression of the gene (bglBC1) from Bacillus circulans encoding an endo-beta-(1-->3),(1-->4)-glucanase useful for the preparation of oligosaccharides from barley beta-glucan
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26
1749-1755
2004
Niallia circulans, Niallia circulans ATCC 21367
brenda
Araki, R.; Ali, M.K.; Sakka, M.; Kimura, T.; Sakka, K.; Ohmiya, K.
Essential role of the family-22 carbohydrate-binding modules for beta-1,3-1,4-glucanase activity of Clostridium stercorarium Xyn10B
FEBS Lett.
561
155-158
2004
Thermoclostridium stercorarium
brenda
Tsai, L.C.; Shyur, L.F.; Cheng, Y.S.; Lee, S.H.
Crystal structure of truncated Fibrobacter succinogenes 1,3-1,4-beta-D-glucanase in complex with beta-1,3-1,4-cellotriose
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354
642-651
2005
Fibrobacter succinogenes
brenda
Gaiser, O.J.; Piotukh, K.; Ponnuswamy, M.N.; Planas, A.; Borriss, R.; Heinemann, U.
Structural basis for the substrate specificity of a Bacillus 1,3-1,4-beta-glucanase
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357
1211-1225
2006
Paenibacillus macerans
brenda
Vasur, J.; Kawai, R.; Larsson, A.M.; Igarashi, K.; Sandgren, M.; Samejima, M.; Stahlberg, J.
X-ray crystallographic native sulfur SAD structure determination of laminarinase Lam16A from Phanerochaete chrysosporium
Acta Crystallogr. Sect. D
62
1422-1429
2006
Phanerodontia chrysosporium
brenda
Kawai, R.; Igarashi, K.; Yoshida, M.; Kitaoka, M.; Samejima, M.
Hydrolysis of beta -1,3/1,6-glucan by glycoside hydrolase family 16 endo-1,3(4)-beta-glucanase from the basidiomycete Phanerochaete chrysosporium
Appl. Microbiol. Biotechnol.
71
898-906
2006
Phanerodontia chrysosporium (Q874E3), Phanerodontia chrysosporium
brenda
Teng, D.; Wang, J.H.; Fan, Y.; Yang, Y.L.; Tian, Z.G.; Luo, J.; Yang, G.P.; Zhang, F.
Cloning of beta-1,3-1,4-glucanase gene from Bacillus licheniformis EGW039 (CGMCC 0635) and its expression in Escherichia coli BL21 (DE3)
Appl. Microbiol. Biotechnol.
72
705-712
2006
Bacillus licheniformis, Bacillus licheniformis EGW039 / CGMCC 0635
brenda
Teng, D.; Fan, Y.; Yang, Y.L.; Tian, Z.G.; Luo, J.; Wang, J.H.
Codon optimization of Bacillus licheniformis beta-1,3-1,4-glucanase gene and its expression in Pichia pastoris
Appl. Microbiol. Biotechnol.
74
1074-1083
2007
Bacillus licheniformis (Q8GMY0), Bacillus licheniformis
brenda
Boyce, A.; Walsh, G.
Production, purification and application-relevant characterisation of an endo-1,3(4)-beta-glucanase from Rhizomucor miehei
Appl. Microbiol. Biotechnol.
76
835-841
2007
Rhizomucor miehei
brenda
Tamoi, M.; Kurotaki, H.; Fukamizo, T.
beta-1,4-Glucanase-like protein from the cyanobacterium Synechocystis PCC6803 is a beta-1,3-1,4-glucanase and functions in salt stress tolerance
Biochem. J.
405
139-146
2007
Synechocystis sp. PCC 6803 (Q55365), Synechocystis sp. PCC 6803
brenda
Gargallo, R.; Cedano, J.; Mozo-Villarias, A.; Querol, E.; Oliva, B.
Study of the influence of temperature on the dynamics of the catalytic cleft in 1,3-1,4-beta-glucanase by molecular dynamics simulations
J. Mol. Model.
12
835-845
2006
Bacillus licheniformis
brenda
Money, V.A.; Cartmell, A.; Guerreiro, C.I.; Ducros, V.M.; Fontes, C.M.; Gilbert, H.J.; Davies, G.J.
Probing the beta-1,3:1,4 glucanase, CtLic26A, with a thio-oligosaccharide and enzyme variants
Org. Biomol. Chem.
6
851-853
2008
Acetivibrio thermocellus
brenda
Allardyce, B.J.; Linton, S.M.
Purification and characterisation of endo-beta-1,4-glucanase and laminarinase enzymes from the gecarcinid land crab Gecarcoidea natalis and the aquatic crayfish Cherax destructor
J. Exp. Biol.
211
2275-2287
2008
Cherax destructor, Cherax destructor Clark 1936
brenda
Cheng, Y.M.; Hong, T.Y.; Liu, C.C.; Meng, M.
Cloning and functional characterization of a complex endo-beta-1,3-glucanase from Paenibacillus sp.
Appl. Microbiol. Biotechnol.
81
1051-1061
2009
Paenibacillus sp. CCRC 17245 (Q000P7)
brenda
Martin-Cuadrado, A.B.; Encinar del Dedo, J.; de Medina-Redondo, M.; Fontaine, T.; del Rey, F.; Latge, J.P.; Vazquez de Aldana, C.R.
The Schizosaccharomyces pombe endo-1,3-beta-glucanase Eng1 contains a novel carbohydrate binding module required for septum localization
Mol. Microbiol.
69
188-200
2008
Schizosaccharomyces pombe
brenda
Qiao, J.; Dong, B.; Li, Y.; Zhang, B.; Cao, Y.
Cloning of a beta-1,3-1,4-glucanase gene from Bacillus subtilis MA139 and its functional expression in Escherichia coli
Appl. Biochem. Biotechnol.
152
334-342
2009
Bacillus subtilis (A8CGP1), Bacillus subtilis MA139 (A8CGP1), Bacillus subtilis MA139
brenda
Kumagai, Y.; Ojima, T.
Enzymatic properties and the primary structure of a beta-1,3-glucanase from the digestive fluid of the Pacific abalone Haliotis discus hannai
Comp. Biochem. Physiol. B
154
113-120
2009
Haliotis discus hannai
brenda
Kumagai, Y.; Ojima, T.
Isolation and characterization of two types of beta-1,3-glucanases from the common sea hare Aplysia kurodai
Comp. Biochem. Physiol. B
155
138-144
2010
Aplysia kurodai
brenda
Encinar del Dedo, J.; Duenas, E.; Arnaiz, Y.; del Rey, F.; Vazquez de Aldana, C.R.
beta-glucanase Eng2 is required for ascus wall endolysis after sporulation in the fission yeast Schizosaccharomyces pombe
Eukaryot. Cell
8
1278-1286
2009
Schizosaccharomyces pombe
brenda
Guo, Q.; Zhang, W.; Ma, L.L.; Chen, Q.H.; Chen, J.C.; Zhang, H.B.; Ruan, H.; He, G.Q.
A food-grade industrial arming yeast expressing beta-1,3-1,4-glucanase with enhanced thermal stability
J. Zhejiang Univ. Sci. B
11
41-51
2010
Bacillus subtilis
brenda
Li, X.; Huang, X.; Shao, X.; Li, L.
Functional cell surface display of endo-beta-1,3-1,4-glucanase in Lactococcus lactis using N-acetylmuraminidase as the anchoring motif
Sheng Wu Gong Cheng Xue Bao
25
89-94
2009
Bacillus subtilis, Bacillus subtilis BF7658
brenda
Bai, Y.; Wang, J.; Zhang, Z.; Shi, P.; Luo, H.; Huang, H.; Luo, C.; Yao, B.
A novel family 9 beta-1,3(4)-glucanase from thermoacidophilic Alicyclobacillus sp. A4 with potential applications in the brewing industry
Appl. Microbiol. Biotechnol.
87
251-259
2010
Alicyclobacillus sp. (D7PC10)
brenda
Vasur, J.; Kawai, R.; Jonsson, K.H.; Widmalm, G.; Engstroem, A.; Frank, M.; Andersson, E.; Hansson, H.; Forsberg, Z.; Igarashi, K.; Samejima, M.; Sandgren, M.; Stahlberg, J.
Synthesis of cyclic beta-glucan using laminarinase 16A glycosynthase mutant from the basidiomycete Phanerochaete chrysosporium
J. Am. Chem. Soc.
132
1724-1730
2010
Phanerodontia chrysosporium (Q874E3), Phanerodontia chrysosporium
brenda
Bleicher, L.; Prates, E.T.; Gomes, T.C.; Silveira, R.L.; Nascimento, A.S.; Rojas, A.L.; Golubev, A.; Martinez, L.; Skaf, M.S.; Polikarpov, I.
Molecular basis of the thermostability and thermophilicity of laminarinases: X-ray structure of the hyperthermostable laminarinase from Rhodothermus marinus and molecular dynamics simulations
J. Phys. Chem. B
115
7940-7949
2011
Rhodothermus marinus (O52754)
brenda
Dvortsov, I.; Lunina, N.; Zverlov, V.; Velikodvorskaia, G.
Substrate-binding properties of family 54 module of laminarinase Lic16A of Clostridium thermocellum
Mol. Biol. (Mosk.)
44
671-676
2010
Acetivibrio thermocellus (Q59328)
brenda
Wang, K.; Luo, H.; Bai, Y.; Shi, P.; Huang, H.; Xue, X.; Yao, B.
A thermophilic endo-1,4-beta-glucanase from Talaromyces emersonii CBS394.64 with broad substrate specificity and great application potentials
Appl. Microbiol. Biotechnol.
98
7051-7060
2014
Rasamsonia emersonii (X4YXV3), Rasamsonia emersonii, Rasamsonia emersonii CBS394.64 (X4YXV3)
brenda
Masilamani, R.; Sharma, O.P.; Muthuvel, S.K.; Natarajan, S.
Cloning, expression of beta-1,3-1,4 glucanase from Bacillus subtilis SU40 and the effect of calcium ion on the stability of recombinant enzyme: in vitro and in silico analysis
Bioinformation
9
958-962
2013
Bacillus licheniformis (Q84GK1)
brenda
Na, H.B.; Jung, W.K.; Jeong, Y.S.; Kim, H.J.; Kim, S.K.; Kim, J.; Yun, H.D.; Lee, J.K.; Kim, H.
Characterization of a GH family 8 beta-1,3-1,4-glucanase with distinctive broad substrate specificity from Paenibacillus sp. X4
Biotechnol. Lett.
37
643-655
2015
Paenibacillus sp. (A0A088BCU2)
brenda
Britto, D.S.; Pirovani, C.P.; Andrade, B.S.; Dos Santos, T.P.; Pungartnik, C.; Cascardo, J.C.; Micheli, F.; Gesteira, A.S.
Recombinant beta-1,3-1,4-glucanase from Theobroma cacao impairs Moniliophthora perniciosa mycelial growth
Mol. Biol. Rep.
40
5417-5427
2013
Theobroma cacao
brenda
Kobayashi, T.; Uchimura, K.; Kubota, T.; Nunoura, T.; Deguchi, S.
Biochemical and genetic characterization of beta-1,3 glucanase from a deep subseafloor Laceyella putida
Appl. Microbiol. Biotechnol.
100
203-214
2016
Laceyella putida (A0A0P0UPC6), Laceyella putida JAM FM3001 (A0A0P0UPC6)
brenda
Ahmed, R.; Jain, S.K.; Shukla, P.K.
In-silico characterization of beta-(1,3)-endoglucanase (ENGL1) from Aspergillus fumigatus by homology modeling and docking studies
Bioinformation
9
802-807
2013
Aspergillus fumigatus
brenda
Kuge, T.; Nagoya, H.; Tryfona, T.; Kurokawa, T.; Yoshimi, Y.; Dohmae, N.; Tsubaki, K.; Dupree, P.; Tsumuraya, Y.; Kotake, T.
Action of an endo-beta-1,3(4)-glucanase on cellobiosyl unit structure in barley beta-1,3:1,4-glucan
Biosci. Biotechnol. Biochem.
79
1810-1817
2015
Trichoderma sp.
brenda
Mangan, D.; Liadova, A.; Ivory, R.; McCleary, B.V.
Novel approaches to the automated assay of beta-glucanase and lichenase activity
Carbohydr. Res.
435
162-172
2016
Acetivibrio thermocellus (Q59328)
brenda
Mouyna, I.; Aimanianda, V.; Hartl, L.; Prevost, M.C.; Sismeiro, O.; Dillies, M.A.; Jagla, B.; Legendre, R.; Coppee, J.Y.; Latge, J.P.
GH16 and GH81 family beta-(1,3)-glucanases in Aspergillus fumigatus are essential for conidial cell wall morphogenesis
Cell. Microbiol.
18
1285-1293
2016
Aspergillus fumigatus (Q4WEA1), Aspergillus fumigatus (Q4WJP2), Aspergillus fumigatus (Q4WQM7), Aspergillus fumigatus (Q4X084), Aspergillus fumigatus ATCC MYA-4609 (Q4WEA1), Aspergillus fumigatus ATCC MYA-4609 (Q4WJP2), Aspergillus fumigatus ATCC MYA-4609 (Q4WQM7), Aspergillus fumigatus ATCC MYA-4609 (Q4X084)
brenda
Sakdapetsiri, C.; Fukuta, Y.; Aramsirirujiwet, Y.; Shirasaka, N.; Kitpreechavanich, V.
Antagonistic activity of endo-beta-1,3-glucanase from a novel isolate, Streptomyces sp. 9X166, against black rot in orchids
J. Basic Microbiol.
56
469-479
2016
Streptomyces sp.
brenda
Merzlov, D.A.; Zorov, I.N.; Dotsenko, G.S.; Denisenko, Y.A.; Rozhkova, A.M.; Satrutdinov, A.D.; Rubtsova, E.A.; Kondratieva, E.G.; Sinitsyn, A.P.
Properties of enzyme preparations and homogeneous enzymes - endoglucanases EG2 Penicillium verruculosum and LAM Myceliophthora thermophila
Biochemistry (Moscow)
80
473-482
2015
Thermothelomyces heterothallicus
brenda
Murray, P.G.; Grassick, A.; Laffey, C.D.; Cuffe, M.M.; Higgins, T.; Savage, A.V.; Planas, A.; Tuohy, M.G.
Isolation and characterization of a thermostable endo-beta-glucanase active on 1,3-1,4-beta-D-glucans from the aerobic fungus Talaromyces emersonii CBS 814.70
Enzyme Microb. Technol.
29
90-98
2001
Rasamsonia emersonii, Rasamsonia emersonii CBS 814.70
brenda
Saraihom, S.; Kobayashi, D.; Lotrakul, P.; Prasongsuk, S.; Eveleigh, D.; Punnapayak, H.
First report of a tropical Lysobacter enzymogenes producing bifunctional endoglucanase activity towards carboxymethylcellulose and chitosan
Ann. Microbiol.
66
907-919
2016
Lysobacter enzymogenes (A0A0K2GVV7), Lysobacter enzymogenes 521 (A0A0K2GVV7)
-
brenda
Kim, D.; Lee, M.; Cho, H.; Lee, J.; Lee, M.; Chung, C.; Shin, D.; Rhee, Y.; Son, K.; Park, H.
Genetic and functional characterization of an extracellular modular GH6 endo-beta-1,4-glucanase from an earthworm symbiont, Cellulosimicrobium funkei HY-13
Antonie van Leeuwenhoek
109
1-12
2016
Cellulosimicrobium funkei (A0A0P0IZM9), Cellulosimicrobium funkei HY-13 (A0A0P0IZM9)
brenda
Meng, D.D.; Wang, B.; Ma, X.Q.; Ji, S.Q.; Lu, M.; Li, F.L.
Characterization of a thermostable endo-1,3(4)-beta-glucanase from Caldicellulosiruptor sp. strain F32 and its application for yeast lysis
Appl. Microbiol. Biotechnol.
100
4923-4934
2016
Caldicellulosiruptor sp. F32 (A0A0S2UQ60)
brenda
Wang, J.; Kang, L.; Liu, Z.; Yuan, S.
Gene cloning, heterologous expression and characterization of a Coprinopsis cinerea endo-beta-1,3(4)-glucanase
Fungal Biol.
121
61-68
2017
Coprinopsis cinerea (A8N2G5), Coprinopsis cinerea ATCC MYA-4618 (A8N2G5)
brenda
Li, J.; Xu, X.; Shi, P.; Liu, B.; Zhang, Y.; Zhang, W.
Overexpression and characterization of a novel endo-beta-1,3(4)-glucanase from thermophilic fungus Humicola insolens Y1
Protein Expr. Purif.
138
63-68
2017
Humicola insolens (A0A0U2RV57), Humicola insolens Y1 (A0A0U2RV57)
brenda