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Information on EC 3.2.1.74 - glucan 1,4-beta-glucosidase
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EC Tree
3.2.1.74 glucan 1,4-beta-glucosidaseIUBMB Comments
Acts on 1,4-beta-D-glucans and related oligosaccharides. Cellobiose is hydrolysed, but very slowly.
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Word Map
- 3.2.1.74
- endoglucanase
- cellulases
- cellobiose
- trichoderma
-
cellulolytic
- reesei
- xylanase
- avicel
-
lignocellulosic
-
cellulosic
-
saccharification
- cmcase
-
microcrystalline
- straw
-
cellulose-binding
- xylan
-
3.2.1.21
- cellulosome
- fusca
- thermocellum
- cellotetraose
- beta-d-glucoside
- thermobifida
- cellulomonas
- endocellulase
- bagasse
-
cellulose-degrading
- cellopentaose
- hypocrea
- jecorina
-
acid-swollen
- cellodextrins
- fibrobacter
- endo-beta-1,4-glucanase
- endoxylanase
- avicelase
- myrosinase
-
fpase
- carboxymethylcellulase
- neocallimastix
- endo-1,4-beta-glucanase
-
lignocellulolytic
- cellooligosaccharides
- flavefaciens
-
dockerins
- humicola
- biofuel production
- biotechnology
-
cellulase-producing
- ruminicola
- insolens
- analysis
- energy production
- cellulovorans
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
cellobiohydrolase, exoglucanase, cellobiase, glucohydrolase, carboxymethyl cellulase, cel9a, exocellulase, cel48a, endo-1,4-beta-d-glucanase, beta-1,4-glucosidase, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
1,4-beta-D-glucan glucanohydrolase
b-glucan glucohydrolase
beta-(1-4)-glucanase
beta-1,4-beta-glucanase
-
-
-
-
beta-1,4-glucosidase
beta-glucosidase
beta-glucosidase/xylosidase
bifunctional, belongs to to the glycoside hydrolase 3 familiy, GH3
Bgl
BglA
cellobiase
Cellulase
endo-1,4-beta-D-glucanase
endo/exocellulase
exo-1,4-beta-glucanase
exo-1,4-beta-glucosidase
-
-
-
-
exo-beta-1,4-glucanase
exo-beta-1,4-glucosidase
-
-
-
-
exocellulase
family-1 glycosyl hydrolase
FiG
glucan 1,4-beta-glucosidase
NA23_08975
Rubg3A
gene name, together with Rubg3B, beta-glucosidase/xylosidase activity, EC 3.2.1.37 and 3.2.1.74
Rubg3B
gene name, together with Rubg3A, beta-glucosidase/xylosidase activity, EC 3.2.1.37 and 3.2.1.74
Xyn10E
b-glucan glucohydrolase
extracellular non-cellulosomal beta-glucan glucohydrolase
b-glucan glucohydrolase
extracellular non-cellulosomal beta-glucan glucohydrolase
-
beta-1,4-glucosidase
Vibrio cholerae serotype O1 E1 Tor N16961
-
-
-
beta-glucosidase
-
-
-
-
beta-glucosidase
Vibrio cholerae serotype O1 E1 Tor N16961
-
-
-
BglA
Vibrio cholerae serotype O1 E1 Tor N16961
-
-
-
cellobiase
Vibrio cholerae serotype O1 E1 Tor N16961
-
-
-
exo-1,4-beta-glucanase
-
-
-
-
exo-beta-1,4-glucanase
-
-
-
-
exocellulase
-
-
-
-
Xyn10E
-
bifunctional recombinant enzyme with xylanase and beta-glucanase activities
Xyn10E
-
bifunctional recombinant enzyme with xylanase and beta-glucanase activities
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
(Glcbeta(1-4))r + H2O = (Glcbeta(1-4))r-1 + beta-D-glucopyranose
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of O-glycosyl bond
hydrolysis of O-glycosyl bond
-
-
-
-
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SYSTEMATIC NAME
IUBMB Comments
4-beta-D-glucan glucohydrolase
Acts on 1,4-beta-D-glucans and related oligosaccharides. Cellobiose is hydrolysed, but very slowly.
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CAS REGISTRY NUMBER
COMMENTARY
37288-52-1
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2-nitrophenyl beta-cellobioside + H2O
2-nitrophenol + beta-cellobiose
-
3.1% activity compared to 4-nitrophenyl beta-D-glucopyranoside
-
-
?
4-methylumbelliferyl 4-O-(beta-D-glucopyranosyl)-beta-D-xylopyranoside + H2O
?
-
-
-
-
?
4-methylumbelliferyl beta-cellotrioside + H2O
4-methylumbelliferone + cellotriose
-
-
-
-
?
4-methylumbelliferyl beta-D-cellobioside + 2 H2O
4-methylumbelliferol + 2 D-glucose
i.e.MuC, 14.9% relative activity compared to 4-nitrophenyl-beta-D-glucopyranoside as substrate
-
-
?
4-methylumbelliferyl beta-D-cellotrioside + H2O
4-methylumbelliferone + cellotriose
-
-
-
?
4-methylumbelliferyl beta-D-glucopyranoside + H2O
4-methylumbelliferone + D-glucopyranose
-
-
-
-
?
4-methylumbelliferyl-beta-D-cellobioside + H2O
D-glucose + ?
i.e.MuC, 157.7% relative activity compared to 4-nitrophenyl beta-D-glucopyranoside as substrate
-
-
?
4-methylumbelliferyl-beta-D-lactoside + H2O
4-methylumbelliferone + D-lactose
-
-
-
-
?
4-nitrophenyl beta-cellobioside + H2O
4-nitrophenol + beta-cellobiose
-
6.5% activity compared to 4-nitrophenyl beta-D-glucopyranoside
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactopyranose
-
1.2% activity compared to 4-nitrophenyl beta-D-glucopyranoside
-
-
?
4-nitrophenyl beta-D-glucopyranose + H2O
4-nitrophenol + D-glucose
-
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucose
-
-
-
?
acid swollen cellulose + H2O
D-glucose + ?
-
-
-
?
alpha-cellulose + H2O
?
-
no activity with carboxymethylcellulose, salicin, cellobiose and alpha-dimethylglucoside
-
-
?
avicel + H2O
cellobiose + ?
-
0.09% activity compared to carboxymethylcellulose
-
-
?
barley beta-D-glucan + H2O
D-glucose
-
strongest activity (15525% activity compared to carboxymethyl cellulose)
-
-
?
barley glucan + H2O
?
-
326% activity compared to carboxymethylcellulose
-
-
?
carboxymethyl cellulose + H2O
cellobiose + cellopentaose + cellotetraose + cellotriose + D-glucose
-
-
the major product is cellobiose with some minor products
-
?
cellohexaose + H2O
cellopentaose + D-glucose
-
-
-
?
cellotetraose + H2O
cellobiose + cellotriose + D-glucose
-
-
cellobiose is the dominant product, while cellotriose and glucose are minor products
-
?
cellotetraose + H2O
glucose + cellobiose + cellotriose
-
with a conversion ratio of 80%
-
?
filter paper + H2O
cellobiose + cellopentaose + cellotetraose + cellotriose + D-glucose
-
-
the major product is cellobiose with some minor products
-
?
hydroquinone-beta-D-glucopyranoside + H2O
hydroquinone + D-glucose
-
-
-
?
hydroxyethyl cellulose + H2O
?
-
220% activity compared to carboxymethyl cellulose
-
-
?
n-heptyl-beta-D-glucopyranoside + H2O
n-heptanol + D-glucose
-
-
-
?
n-hexyl-beta-D-glucopyranoside + H2O
n-hexanol + D-glucose
-
-
-
?
n-octyl-beta-D-glucopyranoside + H2O
n-octanol + D-glucose
-
-
-
?
n-pentyl-beta-D-glucopyranoside + H2O
n-pentanol + D-glucose
-
-
-
?
oat spelt xylan + H2O
?
-
57% activity compared to carboxymethyl cellulose
-
-
?
p-nitrophenol beta-D-cellobioside + H2O
p-nitrophenol beta-D-glucopyranoside + D-glucose
-
-
-
?
p-nitrophenyl glucoside + H2O
p-nitrophenyl + glucose
-
p-nitrophenyl releasing with no lag period, no activity with p-nitrophenyl-?-(N-acetylgalactosamine), p-nitrophenyl-?-glucose-6-phosphate, p-nitrophenyl-(N-acetylglucosamine), p-nitrophenyl-di(N-acetylglucosamine), p-nitrophenyl-tri(N-acetylglucosamine)
-
-
?
p-nitrophenyl-beta-D-cellobiose + H2O
p-nitrophenol + cellobioside
-
-
-
?
p-nitrophenyl-beta-D-cellobioside + H2O
cellobiose + p-nitrophenol
-
-
-
ir
p-nitrophenyl-beta-D-cellotrioside + H2O
D-glucose + p-nitrophenyl-beta-D-cellobioside + p-nitrophenyl-beta-D-cellotetraoside + p-nitrophenyl-beta-D-glucoside
p-nitrophenyl-beta-D-fucopyranoside + H2O
p-nitrophenol + fucopyranose
-
-
-
?
phosphate acid swollen cellulose + H2O
?
-
10.7% activity compared to carboxymethylcellulose
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + D-galactose
0.8% relative activity compared to 4-nitrophenyl beta-D-glucopyranoside as substrate
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + D-galactose
1.3% relative activity compared to 4-nitrophenyl beta-D-glucopyranoside as substrate
-
-
?
2-nitrophenyl beta-D-glucopyranoside + H2O
2-nitrophenol + D-glucopyranose
-
25.2% activity compared to 4-nitrophenyl beta-D-glucopyranoside
-
-
?
2-nitrophenyl beta-D-glucopyranoside + H2O
2-nitrophenol + D-glucopyranose
-
25.2% activity compared to 4-nitrophenyl beta-D-glucopyranoside
-
-
?
4-methylumbelliferyl beta-cellobioside + H2O
4-methylumbelliferone + cellobiose
-
-
-
-
?
4-methylumbelliferyl beta-cellobioside + H2O
4-methylumbelliferone + cellobiose
-
-
-
-
?
4-methylumbelliferyl beta-D-glucoside + H2O
4-methylumbelliferone + D-glucopyranose
-
-
-
-
?
4-methylumbelliferyl beta-D-glucoside + H2O
4-methylumbelliferone + D-glucopyranose
-
-
-
-
?
4-methylumbelliferyl beta-D-glucoside + H2O
4-methylumbelliferone + D-glucopyranose
-
-
-
-
?
4-nitrophenyl alpha-D-glucopyranoside + H2O
4-nitrophenol + D-glucose
-
-
-
-
?
4-nitrophenyl alpha-D-glucopyranoside + H2O
4-nitrophenol + D-glucose
[Humicola] grisea var. thermoidea IFO 9854
-
-
-
-
?
4-nitrophenyl beta-D-cellobioside + H2O
4-nitrophenol + beta-D-cellobiose
-
exo-beta-1,4-glucanase activity, 24.1% activity compared to carboxymethylcellulose
-
-
?
4-nitrophenyl beta-D-cellobioside + H2O
4-nitrophenol + beta-D-cellobiose
-
-
-
-
?
4-nitrophenyl beta-D-cellobioside + H2O
4-nitrophenol + beta-D-cellobiose
[Humicola] grisea var. thermoidea IFO 9854
-
-
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucopyranose
-
100% activity
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucopyranose
-
100% activity
-
-
?
4-nitrophenyl cellobioside + H2O
4-nitrophenol + cellobiose
-
2756% activity compared to carboxymethyl cellulose
-
-
?
4-nitrophenyl cellobioside + H2O
4-nitrophenol + cellobiose
-
2756% activity compared to carboxymethyl cellulose
-
-
?
Avicel + H2O
D-glucose + cellotetraose + cellotriose + cellobiose
-
-
-
?
Avicel + H2O
D-glucose + cellotetraose + cellotriose + cellobiose
-
-
-
-
?
Avicel + H2O
D-glucose + cellotetraose + cellotriose + cellobiose
Sporotrichum pulverulentum
-
-
-
-
?
Avicel + H2O
D-glucose + cellotetraose + cellotriose + cellobiose
-
-
D-glucose only
?
Avicel + H2O
D-glucose + cellotetraose + cellotriose + cellobiose
-
H3PO4 or HCl swollen
cellobiose and cellotriose only
?
carboxymethyl cellulose + H2O
?
-
-
-
?
carboxymethylcellulose + H2O
?
-
endo-beta-1,4-glucanase activity, 100% activity
-
-
?
cellobiose + H2O
2 beta-D-glucose
-
0.96% activity compared to 4-nitrophenyl beta-D-glucopyranoside
-
-
?
cellobiose + H2O
2 beta-D-glucose
-
0.96% activity compared to 4-nitrophenyl beta-D-glucopyranoside
-
-
?
cellobiose + H2O
2 D-glucose
is degraded very slowly
-
-
?
cellobiose + H2O
2 D-glucose
128.8% relative activity compared to 4-nitrophenyl beta-D-glucopyranoside as substrate
cellobiose is completely hydrolyzed to glucose, evaluated by chromatographic analysis
-
?
cellobiose + H2O
2 D-glucose
6.9% relative activity compared to 4-nitrophenyl-beta-D-glucopyranoside as substrate
31.5% cellobiose is hydrolyzed to glucose, evaluated by chromatographic analysis
-
?
cellobiose + H2O
2 D-glucose
-
no activity with di(N-acetylglucosamine), chitobiose, chitin oligosaccharides, trehalose, lactose, maltose, sucrose, sophorose laminaribiose and gentiobiose
-
-
?
cellobiose + H2O
2 D-glucose
Vibrio cholerae serotype O1 E1 Tor N16961
-
no activity with di(N-acetylglucosamine), chitobiose, chitin oligosaccharides, trehalose, lactose, maltose, sucrose, sophorose laminaribiose and gentiobiose
-
-
?
cellobiose + H2O
D-glucose + cellotetraose + cellotriose
-
glucosyl transfer reaction
-
-
?
cellobiose + H2O
D-glucose + cellotetraose + cellotriose
-
only isoenzyme I
-
-
?
cellobiose + H2O
D-glucose + cellotetraose + cellotriose
-
-
-
-
?
cellobiose + H2O
D-glucose + cellotetraose + cellotriose
-
-
D-glucose only
?
cellobiose + H2O
D-glucose + cellotetraose + cellotriose
-
-
-
-
ir
cellobiose + H2O
D-glucose + cellotetraose + cellotriose
-
-
-
?
cellobiose + H2O
D-glucose + cellotetraose + cellotriose
-
-
-
-
?
cellodextrins + H2O
D-glucose + cellotriose + cellobiose
-
-
-
?
cellodextrins + H2O
D-glucose + cellotriose + cellobiose
-
-
at high substrate concentrations also transglycosylation reactions
?
cellodextrins + H2O
D-glucose + cellotriose + cellobiose
-
-
D-glucose only
?
cellohexaose + H2O
D-glucose + ?
-
-
in the early stages also cellopentaose and cellotetraose
?
cellohexaose + H2O
D-glucose + ?
-
-
cellotetraose, cellotriose and cellobiose only
?
cellopentaose + H2O
D-glucose + ?
-
-
cellobiose and cellotriose only
?
cellotetraose + H2O
?
no activity with cellotriose and chitin
-
-
?
cellotetraose + H2O
D-glucose + cellotriose + cellobiose
-
highest substrate preference for cellotriose and cellotetraose
-
-
?
cellotetraose + H2O
D-glucose + cellotriose + cellobiose
-
-
-
?
cellotetraose + H2O
D-glucose + cellotriose + cellobiose
-
-
-
-
ir
cellotetraose + H2O
D-glucose + cellotriose + cellobiose
-
-
-
-
?
cellotetraose + H2O
D-glucose + cellotriose + cellobiose
-
-
cellobiose only
?
cellotetraose + H2O
D-glucose + cellotriose + cellobiose
-
-
-
-
?
cellotriose + H2O
D-glucose + cellobiose
-
highest substrate preference for cellotriose and cellotetraose
-
-
?
cellulose + H2O
D-glucose + ?
-
the glucohydrolase attaches directly to crystalline cellulose to release D-glucose from free chain ends
-
-
?
cellulose + H2O
D-glucose + ?
-
cellulose substrate Sigmacell 20
-
-
?
cellulose + H2O
D-glucose + ?
-
cellulose substrate Sigmacell 20
-
-
?
cellulose + H2O
D-glucose + ?
-
H3PO4 swollen
D-glucose + cellobiose
?
cellulose + H2O
D-glucose + ?
-
bacterial microcrystalline cellulose
-
-
?
cellulose + H2O
D-glucose + ?
-
cellulose HBS and cellulose MN300
-
-
?
cellulose + H2O
D-glucose + ?
-
hydrolysis of natural corncob cellulosic residues
-
-
?
cellulose + H2O
D-glucose + ?
-
hydrolysis of natural corncob cellulosic residues
-
-
?
filter paper + H2O
cellobiose + ?
-
0.02% activity compared to carboxymethylcellulose
-
-
?
galactomannan + H2O
?
-
7363% activity compared to carboxymethyl cellulose
-
-
?
galactomannan + H2O
?
-
7363% activity compared to carboxymethyl cellulose
-
-
?
lichenan + H2O
?
-
315% activity compared to carboxymethylcellulose
-
-
?
o-nitrophenyl-beta-D-galactopyranose + H2O
o-nitrophenol + beta-D-galactopyranose
-
-
-
?
o-nitrophenyl-beta-D-galactopyranose + H2O
o-nitrophenol + beta-D-galactopyranose
-
-
-
?
p-nitrophenyl cellobioside + H2O
p-nitrophenyl + glucose
-
p-nitrophenyl releasing after a lag period
-
-
?
p-nitrophenyl cellobioside + H2O
p-nitrophenyl + glucose
Vibrio cholerae serotype O1 E1 Tor N16961
-
p-nitrophenyl releasing after a lag period
-
-
?
p-nitrophenyl-beta-D-cellobioside + H2O
D-glucose + p-nitrophenyl-beta-D-glucoside
-
-
-
-
?
p-nitrophenyl-beta-D-cellobioside + H2O
D-glucose + p-nitrophenyl-beta-D-glucoside
-
-
-
-
?
p-nitrophenyl-beta-D-cellobioside + H2O
D-glucose + p-nitrophenyl-beta-D-glucoside
-
-
-
-
?
p-nitrophenyl-beta-D-cellobioside + H2O
D-glucose + p-nitrophenyl-beta-D-glucoside
-
-
-
?
p-nitrophenyl-beta-D-cellobioside + H2O
D-glucose + p-nitrophenyl-beta-D-glucoside
-
-
-
-
?
p-nitrophenyl-beta-D-cellobioside + H2O
D-glucose + p-nitrophenyl-beta-D-glucoside
-
-
-
-
?
p-nitrophenyl-beta-D-cellobioside + H2O
D-glucose + p-nitrophenyl-beta-D-glucoside
-
-
-
-
?
p-nitrophenyl-beta-D-cellotrioside + H2O
D-glucose + p-nitrophenyl-beta-D-cellobioside + p-nitrophenyl-beta-D-cellotetraoside + p-nitrophenyl-beta-D-glucoside
-
-
-
?
p-nitrophenyl-beta-D-cellotrioside + H2O
D-glucose + p-nitrophenyl-beta-D-cellobioside + p-nitrophenyl-beta-D-cellotetraoside + p-nitrophenyl-beta-D-glucoside
-
-
-
?
p-nitrophenyl-beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucose
-
-
when incubated with 100 mM substrate, products are also 4-nitrophenyl-beta-laminaribioside, 4-nitrophenyl-beta-cellobioside and 4-nitrophenyl-beta-gentiobioside
?
p-nitrophenyl-beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucose
-
-
-
?
p-nitrophenyl-beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucose
-
-
-
?
p-nitrophenyl-beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucose
-
-
-
?
p-nitrophenyl-beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucose
-
-
-
?
p-nitrophenyl-beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucose
-
-
-
?
p-nitrophenyl-beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucose
-
-
-
?
additional information
?
-
-
no activity with soluble starch, laminarin, and mannan
-
-
?
additional information
?
-
-
no activity with soluble starch, laminarin, and mannan
-
-
?
additional information
?
-
no activity on laminarin, sophorose, laminaribiose, gentiobiose, p-nitrophenol-alpha-L-arabinofuranoside, p-nitrophenol-alpha-D-glucoside, p-nitrophenol-beta-D-cellobioside, p-nitrophenol-beta-D-fucoside, p-nitrophenol-beta-D-galactoside, p-nitrophenol-N-acetyl-beta-D-glucosaminide, p-nitrophenol-beta-D-glucoside, p-nitrophenol-beta-D-glucuronoside, p-nitrophenol-beta-D-lactoside, or p-nitrophenol-beta-D-maltoside
-
-
?
additional information
?
-
-
no activity is detected towards carboxymethyl cellulose
-
-
?
additional information
?
-
-
the enzyme is able to synthesise some cellotetraose by the transglycosylation of smaller substrates
-
-
?
additional information
?
-
-
the recombinant enzyme shows bifunctional degradation activity toward xylan and beta-glucan and also degrades microcrystalline cellulose
-
-
?
additional information
?
-
-
the recombinant enzyme shows bifunctional degradation activity toward xylan and beta-glucan and also degrades microcrystalline cellulose
-
-
?
additional information
?
-
-
no activity with cellotriose, cellotetraose, cellopentaose, carboxy-methylcellulose, xylan, laminarin, avicel (2% w/v), 4-nitrophenyl beta-D-mannopyranoside, and 4-nitrophenyl alpha-D-glucopyranoside
-
-
?
additional information
?
-
-
the enzyme is also able to promote transglycosylation using methanol as an acceptor and glucose as a donor
-
-
?
additional information
?
-
-
no activity with cellotriose, cellotetraose, cellopentaose, carboxy-methylcellulose, xylan, laminarin, avicel (2% w/v), 4-nitrophenyl beta-D-mannopyranoside, and 4-nitrophenyl alpha-D-glucopyranoside
-
-
?
additional information
?
-
-
the enzyme is also able to promote transglycosylation using methanol as an acceptor and glucose as a donor
-
-
?
additional information
?
-
-
endo/exocellulase Cel9A does not fully hydrolyze the cellobiose, even with longer incubations
-
-
?
additional information
?
-
Thermomonospora sp.
-
the enzyme exhibits both endocarboxymethyl cellulase and endoxylanase activity, both substrates are hydrolyzed at different active sites
-
-
?
additional information
?
-
Thermomonospora sp.
-
fluorescence study on interactions of chaperone alpha-crystallin with the molten globule state of the enzyme induced by guanidine hydrochloride
-
-
?
additional information
?
-
-
no activity with laminarin, glucan, birchwood xylan, and 4-nitrophenyl beta-D-glucopyranside
-
-
?
additional information
?
-
-
the cellulase Rucel5B possesses endo-beta-1,4-glucanase activity and exo-beta-1,4-glucanase activity
-
-
?
additional information
?
-
no activity with 4-nitrophenyl alpha-D-glucopyranoside and 4-methylumbelliferyl alpha-D-galactopyranoside as substrates
-
-
?
additional information
?
-
no activity with 4-nitrophenyl alpha-D-glucopyranoside and 4-methylumbelliferyl alpha-D-galactopyranoside as substrates
-
-
?
additional information
?
-
no activity with 4-nitrophenyl alpha-D-glucopyranoside, cellotetraose, and 4-methylumbelliferyl alpha-D-galactopyranoside as substrates
-
-
?
additional information
?
-
no activity with 4-nitrophenyl alpha-D-glucopyranoside, cellotetraose, and 4-methylumbelliferyl alpha-D-galactopyranoside as substrates
-
-
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
CaCl2
Co2+
CoCl2
CuCl2
D-fructose
5 mM, 102.3% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 42°C
D-glucose
5 mM,82% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 42°C
D-xylose
5 mM, 106.3% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 42°C
Fe3+
fructose
5 mM, 107.6% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 40°C
glucose
5 mM,24.5% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 40°C
MgCl2
Mn2+
MnCl2
NiCl2
xylose
5 mM, 88% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 40°C
ZnSO4
additional information
CaCl2
5 mM, 107.3% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 40°C
CaCl2
5 mM, 92.8% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 42°C
Co2+
-
5 min at 50ºC, 1 mM, 103.0% of the relative activity without metal ions
Co2+
-
5 min at 50ºC, 10 mM, 101.9% of the relative activity without metal ions
CoCl2
5 mM, 79.2% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 40°C
CoCl2
5 mM, 85.5% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 42°C
CuCl2
5 mM, 79.5% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 40°C
CuCl2
5 mM, 83.6% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 42°C
Fe3+
-
5 min at 50ºC, 1 mM, 105.5% of the relative activity without metal ions
Fe3+
-
5 min at 50ºC, 10 mM, 103.6% of the relative activity without metal ions
MgCl2
5 mM, 110.1% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 40°C
MgCl2
5 mM, 96.5% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 42°C
Mn2+
-
5 min at 50ºC, 1 mM, 105.8% of the relative activity without metal ions
Mn2+
-
5 min at 50ºC, 10 mM, 104.1% of the relative activity without metal ions
MnCl2
5 mM, 102.4% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 42°C
MnCl2
5 mM, 112% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 40°C
NiCl2
5 mM, 76.7% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 40°C
NiCl2
5 mM, 86.5% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 42°C
ZnSO4
5 mM, 111.4% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 40°C
ZnSO4
5 mM, 90.2% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 42°C
additional information
-
divalent cations are not required for enzyme activation
additional information
not influenced by Zn2+, Ca2+, Mg2+ and Cu2+
additional information
-
the addition of 5 mM Zn2+, Co2+ and Cu2+ has no statistical difference on enzyme activity
additional information
-
NaCl and KCl gives optimum results at 10 mM, at 37ºC, pH 6.5
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Ag+
-
5 min at 50ºC, 10mM, 100.0% of inhibition; 5 min at 50ºC, 1 mM, 100.0% inhibition
Mg2+
-
the exoglucanase activity is reduced by 5-10% in the presence of 5 mM Mg2+
Ca2+
-
5 min at 50ºC, 10 mM, 15.9% inhibition; 5 min at 50ºC, 1 mM, 12.5% inhibition
Ca2+
-
the exoglucanase activity is reduced by 5-10% in the presence of 5 mM Ca2+
Cu2+
-
5 min at 50ºC, 10 mM, 72.0% inhibition; 5 min at 50ºC, 1 mM, 67.8% inhibition
D-glucose
-
D-glucose inhibition is competitive for 4-nitrophenyl beta-D-glucopyranoside
Hg2+
-
5 min at 50ºC, 10 mM, 100.0% inhibition; 5 min at 50ºC, 1 mM, 100.0% inhibition
Ni2+
-
the exoglucanase activity is reduced by 5-10% in the presence of 5 mM Ni2+
Zn2+
-
the exoglucanase activity is reduced by 5-10% in the presence of 5 mM Zn2+
additional information
Cel9D has 27% lower activity on medium-viscosity carboxymethyl cellulose compared to the activity on low-viscosity carboxymethyl cellulose. Is not inhibited by 10 mM EDTA
-
additional information
-
not inhibited by EDTA, N-ethylmaleimide, beta-mercaptoethanol or cysteine. Ca2+, Co2+, Mg2+, Mn2+, and Zn2+ have no inhibitory effect
-
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
ethanol
5% (v/v), 158.4% activity compared to activity without addition of ethanol, 1.25 mM 4-nitrophenyl-beta-D-glucopyranoside as substrate, pH 4.6, 42°C
methanol
5% (v/v), 141% activity compared to activity without addition of methanol, 1.25 mM 4-nitrophenyl-beta-D-glucopyranoside as substrate, pH 4.6, 42°C
additional information
calcium ions are not required for its function
-
additional information
-
thiol groups are not essential for catalytic activity, the enzyme is not affected by Tween 20
-
additional information
-
wild type beta-glucosidase and its mutant S319C enhance activities of endo/exocellulase Cel9A on carboxymethyl cellulose and filter paper substrates
-
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DISEASE
TITLE OF PUBLICATION
LINK TO PUBMED
Foot-and-Mouth Disease
Synergistic effects of 2A-mediated polyproteins on the production of lignocellulose degradation enzymes in tobacco plants.
Gangliosidosis, GM1
Multiple carbohydrate-cleaving specificities in human acidic and neutral glycosidases.
Gaucher Disease
Differences in origin of the 1448C mutation in patients with Gaucher disease.
Gaucher Disease
Human acid beta-glucosidase: use of inhibitors, alternative substrates and amphiphiles to investigate the properties of the normal and Gaucher disease active sites.
Gaucher Disease
Multiple carbohydrate-cleaving specificities in human acidic and neutral glycosidases.
Glycogen Storage Disease
The subcellular distribution of enzymes in type II glycogenosis and the occurrence of an oligo-alpha-1,4-glucan glucohydrolase in human tissues.
Hypertension
Aorta collagen metabolism in spontaneously hypertensive and aortic-constricted rats: variations in enzyme activities concerned with disaccharide unit synthesis and degradation according to blood pressure and age.
Infections
Cel1, probably encoding a cellobiohydrolase lacking the substrate binding domain, is expressed in the initial infection phase of Claviceps purpurea on Secale cereale.
Infections
Ultrastructural and cytochemical investigations of pathogen development and host reactions in susceptible and partially-resistant carrot roots infected by Pythium violae, the major causal agent for cavity spot.
Leukemia, Lymphoid
Neutral maltase: the first human B-cell enzymatic marker reflecting terminal differentiation of mature B cells into plasma cells.
Neoplasms
Synthesis of Novel Fluorine Compounds Substituted-4-thiazolidinones Derived from Rhodanine Drug as Highly Bioactive Probes.
Obesity
[Cellobiohydrolase Activity in the Digestive Tract of two African Rodent Species the African Grass Rat Arvicanthis niloticus and Vlei Rat Otomys helleri].
Starvation
The Aspergillus nidulans signalling mucin MsbA regulates starvation responses, adhesion and affects cellulase secretion in response to environmental cues.
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
1.74
4-nitrophenyl beta-D-cellobioside
-
at 65°C in pH 5.5 citrate buffer
0.22
4-nitrophenyl beta-D-glucopyranoside
pH 4.6, 42°C
1.06
4-nitrophenyl beta-D-glucopyranoside
pH 4.6, 40°C
2.5
4-nitrophenyl beta-D-glucopyranoside
-
in 100 mM sodium acetate buffer (pH 4.5), at 65°C
additional information
additional information
-
laminarioligosaccharide degree of polymerization 2, 5.37, laminarioligosaccharide degree of polymerization 3, 2.77, laminarioligosaccharide degree of polymerization 4, 0.52
-
additional information
additional information
-
cellobiose 15 mg/ml
-
additional information
additional information
-
Walseth cellulose 10 mg/ml, cellopentaose 0.83 mg/ml, cellotriose 0.55 mg/ml, cellotriose 0.55 mg/ml
-
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
69.6
4-nitrophenyl beta-D-cellobioside
-
at 65°C in pH 5.5 citrate buffer
8
Barley beta-glucan
-
at pH 6.0, temperature not specified in the publication
126.6
4-nitrophenyl beta-D-glucopyranoside
pH 4.6, 40°C
203
4-nitrophenyl beta-D-glucopyranoside
pH 4.6, 42°C
4945
4-nitrophenyl beta-D-glucopyranoside
-
in 100 mM sodium acetate buffer (pH 4.5), at 65°C
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
40.1
4-nitrophenyl beta-D-cellobioside
-
at 65°C in pH 5.5 citrate buffer
11900
4-nitrophenyl beta-D-glucopyranoside
pH 4.6, 40°C
915700
4-nitrophenyl beta-D-glucopyranoside
pH 4.6, 42°C
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.04
-
using filter paper as substrate, at 60°C in pH 6.5 citrate buffer
0.067
-
crude enzyme from homogenate, using cellobiose as substrate, pH and temperature not specified in the publication
0.1
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 1 week
0.2
0.228
-
after 3.4fold purification, using cellobiose as substrate, pH and temperature not specified in the publication
0.3
0.35
2-nitrophenyl beta-D-galactopyranoside as substrate, pH 4.6, 40°C
0.4
0.5
0.6
0.65
2-nitrophenyl beta-D-galactopyranoside as substrate, pH 4.6, 42°C
0.7
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 1 week
0.8
0.9
1.1
1.14
-
substrate 4-nitrophenyl alpha-D-glucopyranoside, pH 5.0, 50°C
1.2
1.3
1.4
1.5
1.6
1.7
1.8
1.9
10.7
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 2 weeks
10.9
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 2 weeks
11.5
4-methylumbelliferyl beta-D-cellobioside as substrate, pH 4.6, 42°C
12.2
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 4 weeks
12.4
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 1 week
13.2
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 1 week
130
14.6
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 1 week
2
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 8 weeks
2.1
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 4 weeks
2.2
2.3
2.4
2.6
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 8 weeks
2.9
21.1
22.6
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 2 weeks
220
-
using carboxymethylcellulose as substrate, at 65°C in pH 5.5 citrate buffer
23.3
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 1 week
23.5
-
using phosphate acid swollen cellulose as substrate, at 60°C in pH 6.5 citrate buffer
26
3.1
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 8 weeks
3.2
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 8 weeks
3.3
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 2 weeks
3.4
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 8 weeks
3.7
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 1 week
3.9
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 4 weeks
4
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 4 weeks
4.1
41
4-methylumbelliferyl beta-D-cellobioside as substrate, pH 4.6, 40°C
5.1
52.9
-
using 4-nitrophenyl beta-D-cellobioside as substrate, at 65°C in pH 5.5 citrate buffer
6.8
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 2 weeks
7.9
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 8 weeks
717
-
using barley glucan as substrate, at 60°C in pH 6.5 citrate buffer
77
4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 42°C
8.09
-
substrate 4-nitrophenyl beta-D-cellobioside, pH 5.0, 50°C
8.4
9.4
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 2 weeks
9.5
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 2 weeks
additional information
0.2
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 8 weeks
0.2
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 4 weeks
0.3
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 4 weeks
0.3
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 2 weeks
0.3
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 4 weeks
0.4
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 2 weeks
0.4
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 1 week
0.4
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 2 weeks
0.4
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 1 week
0.5
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 2 weeks
0.5
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 1 week
0.6
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 2 weeks
0.6
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 4 weeks
0.8
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 1 week
0.8
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 8 weeks
0.8
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 2 weeks
0.9
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 1 week
0.9
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 2 weeks
0.9
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 4 weeks
1.1
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 1 week
1.1
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 1 week
1.1
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 8 weeks
1.1
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 2 weeks
1.1
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 8 weeks
1.2
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 4 weeks
1.2
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 1 week
1.2
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 8 weeks
1.2
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 8 weeks
1.3
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 1 week
1.3
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 4 weeks
1.4
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 4 weeks
1.4
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 8 weeks
1.4
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 1 week
1.4
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 8 weeks
1.4
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 8 weeks
1.4
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 2 weeks
1.4
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 2 weeks
1.5
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 8 weeks
1.5
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 4 weeks
1.6
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 4 weeks
1.6
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 1 week
1.7
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 2 weeks
1.7
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 4 weeks
1.7
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 1 week
1.8
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 1 week
1.8
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 8 weeks
1.8
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 4 weeks
1.8
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 8 weeks
1.9
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 2 weeks
1.9
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 2 weeks
1.9
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 2 weeks
2.2
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 4 weeks
2.2
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 8 weeks
2.2
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 8 weeks
2.2
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 1 week
2.2
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 8 weeks
2.3
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 1 week
2.3
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 8 weeks
2.4
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 4 weeks
2.4
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 8 weeks
2.9
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 4 weeks
2.9
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 8 weeks
26
4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 40°C
4.1
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 8 weeks
4.1
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 4 weeks
5
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 2 weeks
5
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 4 weeks
5
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 4 weeks
5.1
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 4 weeks
5.1
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 1 week
8.4
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 1 week
8.4
-
37°C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 1 week
additional information
cloned Cel48A, 0.068 µmol/min/µmol, filter paper, pH 5.5, at 50ºC, liquid assays
additional information
-
cloned Cel48A, 0.068 µmol/min/µmol, filter paper, pH 5.5, at 50ºC, liquid assays
additional information
cloned Cel48A, 0.191 µmol/min/µmol, bacterial microcrystalline cellulose, pH 5.5, at 50ºC, liquid assays
additional information
-
cloned Cel48A, 0.191 µmol/min/µmol, bacterial microcrystalline cellulose, pH 5.5, at 50ºC, liquid assays
additional information
cloned Cel48A, 0.288 µmol/min/µmol, swollen cellulose, pH 5.5, at 50ºC, thin-layer analysis
additional information
-
cloned Cel48A, 0.288 µmol/min/µmol, swollen cellulose, pH 5.5, at 50ºC, thin-layer analysis
additional information
cloned Cel48A, 0.292 µmol/min/µmol, carboxymethyl cellulose, pH 5.5, at 50ºC, liquid assays
additional information
-
cloned Cel48A, 0.292 µmol/min/µmol, carboxymethyl cellulose, pH 5.5, at 50ºC, liquid assays
additional information
cloned Cel48A, 0.405 µmol/min/µmol, swollen cellulose, pH 5.5, at 50ºC, liquid assays
additional information
-
cloned Cel48A, 0.405 µmol/min/µmol, swollen cellulose, pH 5.5, at 50ºC, liquid assays
additional information
cloned Cel48A, approximately 16 µmol/min/µmol, cellotetraose, pH 5.5, at 50ºC, thin-layer analysis
additional information
-
cloned Cel48A, approximately 16 µmol/min/µmol, cellotetraose, pH 5.5, at 50ºC, thin-layer analysis
additional information
cloned Cel48A, approximately 9 µmol/min/µmol, cellopentaose, pH 5.5, at 50ºC, thin-layer analysis
additional information
-
cloned Cel48A, approximately 9 µmol/min/µmol, cellopentaose, pH 5.5, at 50ºC, thin-layer analysis
additional information
native Cel48A, 17.9 µmol/min/µmol, swollen cellulose, pH 5.5, at 50ºC
additional information
-
native Cel48A, 17.9 µmol/min/µmol, swollen cellulose, pH 5.5, at 50ºC
additional information
native Cel48A, 3.7 µmol/min/µmol, carboxymethyl cellulose, pH 5.5, at 50ºC
additional information
-
native Cel48A, 3.7 µmol/min/µmol, carboxymethyl cellulose, pH 5.5, at 50ºC
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
4.5
4.6
5 - 6
6 - 6.5
6.5
4.6
assay at, optimal for 4-nitrophenol beta-D-glucopyranoside as substrate
4.6
assay at, optimal for 4-nitrophenyl beta-D-glucopyranoside as substrate
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
4 - 5
-
84% and 86% of the maximum activity appears at pH 4.0 and 5.0, respectively
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
37
40
42
assay at, optimal for 4-nitrophenyl beta-D-glucopyranoside as substrate
45
50
60
65
70
40
assay at, optimal for 4-nitrophenyl beta-D-glucopyranoside
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TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
50 - 100
-
about 30% activity at 50°C about 55% activity at 60°C, about 80% activity at 70°C, 100% activity at 80-90°C, about 30% activity at 100°C
60 - 70
-
85% and 91% of the maximum activity at 60°C and 70°C, respectively
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pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Rubg3A, together with Rubg3B, beta-glucosidase/xylosidase activity, EC 3.2.1.37 and 3.2.1.74; isolated from rumen from a yak rumen metagenomic library, chinese yak, Bos grunniens
UniProt
brenda
Rubg3B, together with Rubg3A, beta-glucosidase/xylosidase activity, EC 3.2.1.37 and 3.2.1.74; isolated from rumen from a yak rumen metagenomic library, chinese yak, Bos grunniens
UniProt
brenda
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
additional information
additional information
isolated from rumen from a yak rumen metagenomic library, chinese yak, Bos grunniens
brenda
additional information
isolated from rumen from a yak rumen metagenomic library, chinese yak, Bos grunniens
brenda
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
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UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). BGLA_THENN
Thermotoga neapolitana (strain ATCC 49049 / DSM 4359 / NBRC 107923 / NS-E)
444
0
51535
Swiss-Prot
-
A0A0A6U231_XANCH
870
0
91631
TrEMBL
-
A0A0H3C7X7_CAUVN
Caulobacter vibrioides (strain NA1000 / CB15N)
826
0
85992
TrEMBL
-
A0A0H3C9L1_CAUVN
Caulobacter vibrioides (strain NA1000 / CB15N)
821
0
86499
TrEMBL
-
Q21P70_SACD2
Saccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024)
862
0
92939
TrEMBL
-
A0A645EX04_9ZZZZ
113
0
12622
TrEMBL
other Location (Reliability: 1)
Q21HS2_SACD2
Saccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024)
1072
0
115968
TrEMBL
-
Q084Z4_SHEFN
Shewanella frigidimarina (strain NCIMB 400)
880
0
94719
TrEMBL
-
A0A6M1ZI49_9BACT
573
0
66083
TrEMBL
-
B3PBS6_CELJU
Cellvibrio japonicus (strain Ueda107)
1069
1
117006
TrEMBL
-
A4FNP6_SACEN
Saccharopolyspora erythraea (strain ATCC 11635 / DSM 40517 / JCM 4748 / NBRC 13426 / NCIMB 8594 / NRRL 2338)
615
1
65312
TrEMBL
-
D5ETZ7_PRER2
Prevotella ruminicola (strain ATCC 19189 / JCM 8958 / 23)
767
0
83532
TrEMBL
-
D5EXY0_PRER2
Prevotella ruminicola (strain ATCC 19189 / JCM 8958 / 23)
787
0
84920
TrEMBL
-
A0A2N9D941_9XANT
888
0
93831
TrEMBL
-
Q3BUK9_XANC5
Xanthomonas campestris pv. vesicatoria (strain 85-10)
888
0
93724
TrEMBL
-
A0A645J287_9ZZZZ
87
0
9751
TrEMBL
other Location (Reliability: 1)
A0A645FIR2_9ZZZZ
207
0
24434
TrEMBL
other Location (Reliability: 3)
A3D1P0_SHEB5
Shewanella baltica (strain OS155 / ATCC BAA-1091)
886
0
94766
TrEMBL
-
Q2G7X2_NOVAD
Novosphingobium aromaticivorans (strain ATCC 700278 / DSM 12444 / CCUG 56034 / CIP 105152 / NBRC 16084 / F199)
811
0
85436
TrEMBL
-
A0A6M1YQ78_9BACT
572
0
66634
TrEMBL
-
Q12KZ2_SHEDO
Shewanella denitrificans (strain OS217 / ATCC BAA-1090 / DSM 15013)
866
0
93334
TrEMBL
-
D5EXF9_PRER2
Prevotella ruminicola (strain ATCC 19189 / JCM 8958 / 23)
779
0
86253
TrEMBL
-
A0A6V7EDW2_9XANT
888
0
93880
TrEMBL
-
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PDB
SCOP
CATH
UNIPROT
ORGANISM
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
53000
65000
87000
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
dimer
homooctamer
additional information
Thermomonospora sp.
-
fluorescence study on interactions of alpha-crystallin with the molten globule state of the enzyme induced by guanidine hydrochloride
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POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
glycoprotein
no modification
proteolytic modification
-
Papain digestion of native Ex-1, core fragment obtaining
no modification
Vibrio cholerae serotype O1 E1 Tor N16961
-
-
-
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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
combination of the immunoglobulin-like module and the catalytic module and complex of inactive mutant with cellotetraose, modified microbatch crystallization under oil at 0ºC
-
molecular docking studies for the enzyme with the substrates cellobiose and 4-nitrophenyl beta-D-glucopyranoside. The essential catalytic residues of RuBG3B are D272 and E481. Besides D88, K193, H194, and R160 also form hydrogen bonds with substrates and are conservative. The active center for beta-glucosidic and beta-xylosidic substrates are the same and the bifunctional activity should come from one functional domain. RuBG3A and RuBG3B exhibit higher scores in the docking analysis against 4-nitrophenyl-beta-D-glucopyranoside than against 4-nitrophenyl-beta-D-xylopyranoside, indicating a larger affinity between the enzymes and 4-nitrophenyl-beta-D-glucopyranoside, which is consistent with the kinetic parameters
molecular docking studies for the enzyme with the substrates cellobiose and 4-nitrophenyl-beta-D-glucopyranoside. The essential catalytic residues of RuBG3A are D297 and E499. Besides D113, K218, H219, and R185 also form hydrogen bonds with substrates and are condervative. The active center for beta-glucosidic and beta-xylosidic substrates are the same and the bifunctional activity should come from one functional domain. RuBG3A and RuBG3B exhibit higher scores in the docking analysis against 4-nitrophenyl-beta-D-glucopyranoside than against 4-nitrophenyl-beta-D-xylopyranoside, indicating a larger affinity between the enzymes and 4-nitrophenyl-beta-D-glucopyranoside, which is consistent with the kinetic parameters
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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
D166A
no activity on either 1% (wt/vol) carboxymethyl cellulose after 4 h of incubation or 5 mM cellopentaose after 24 h of incubation
DELTAS168
no activity on either 1% (wt/vol) carboxymethyl cellulose after 4 h of incubation or 5 mM cellopentaose after 24 h of incubation
E612A
no activity on either 1% (wt/vol) carboxymethyl cellulose after 4 h of incubation or 5 mM cellopentaose after 24 h of incubation
S168A
retains about 40% of activity compared to the wild-type
E495D
-
active site mutant, weaker binding affinities to all tested ligands
E495N
-
active site mutant, weaker binding affinities to all tested ligands
G234S
-
loop mutant, higher binding affinities for 4-methylumbelliferyl beta-cellotrioside and cellotriose
G234S/G284P
-
loop mutant, higher binding affinity for 4-methylumbelliferyl beta-cellotrioside
G284P/A229V
-
loop mutant, weaker binding affinities to all tested ligands
G331A
-
active site mutant, weaker binding affinities to all tested ligands
H326A
-
active site mutant, weaker binding affinities to all tested ligands
H326S
-
active site mutant, higher binding affinities for 4-methylumbelliferyl beta-cellobioside and cellotriose
L230C/D512C
-
loop mutant, weaker binding affinities to all tested ligands
N233C/D506C
-
loop mutant, weaker binding affinities to all tested ligands
W329C
-
active site mutant, weaker binding affinities to all tested ligands
W332A
-
active site mutant, weaker binding affinities to all tested ligands
W332Y
-
active site mutant, weaker binding affinities to all tested ligands
additional information
additional information
-
truncation mutant enzymes X-CBM22, X-CBM3 and X-GH10 show 100%, 25.9% and 26.9% activity with barley beta-glucan, respectively, as compared to the wild type enzyme
additional information
-
truncation mutant enzymes X-CBM22, X-CBM3 and X-GH10 show 100%, 25.9% and 26.9% activity with barley beta-glucan, respectively, as compared to the wild type enzyme
-
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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
2 - 5.5
-
after 24 h of incubation at 20°C, the enzyme is fairly stable at the acidic pH ranging from 3.0 to 5.5. About 15% and 76% of activity remain after incubation at pH 2.0 and 2.5, respectively
713875
5 - 8
5 - 8
Thermomonospora sp.
-
50°C, 1 h, less than 15% loss of activity
663865
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
30
37
45
50
50 - 70
-
at 70°C, 65°C, 60°C, and 50°C the enzyme retains 50% of the initial activity after 2, 10, 16, and 35 min, respectively
70 - 90
-
the hydrolyzing activity is not diminished up to 10 days at 70°C or 75°C. The half-lives of the recombinant enzyme are about 200 and 113 h at 80°C and 85°C, respectively. The enzyme activity is drastically reduced at 90°C
50
Thermomonospora sp.
-
15 h, enzyme retains complete activity of hydrolyzing xylan and carboxymethylcellulose
50
Tm-value, retained 50% activity after 20 min incubation
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ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Ethanol
Methanol
Ethanol
5% (v/v), 100.8% activity compared to activity without addition of ethanol, 1.25 mM 4-nitrophenyl-beta-D-glucopyranoside as substrate, pH 4.6, 40°C
Ethanol
5% (v/v), 158.4% activity compared to activity without addition of ethanol, 1.25 mM 4-nitrophenyl-beta-D-glucopyranoside as substrate, pH 4.6, 42°C
Methanol
5% (v/v), 108.2% activity compared to activity without addition of methanol, 1.25 mM 4-nitrophenyl-beta-D-glucopyranoside as substrate, pH 4.6, 40°C
Methanol
5% (v/v), 141% activity compared to activity without addition of methanol, 1.25 mM 4-nitrophenyl-beta-D-glucopyranoside as substrate, pH 4.6, 42°C
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, Macro-Prep DEAE column chromatography, methyl hydrophobic-interaction column chromatography, and Superdex 200 gel filtration
-
by nickel affinity chromatography and gel filtration chromatography
-
catalytic and cellulose binding domains by cellulose FPLC column chromatography, ultrafiltration and papain treatment
-
centrifugation, streptomycin sulfate precipitation, ammonium sulfate fractionation, DEAE column chromatography, IMAC-Zn2+ chromatography
-
cloned Cel48A, centrifugation, phenylmethanesulfonyl fluoride and ammonium sulfate fractionation, phenyl-Sepharose column, Q-Sepharose column
precipitation with acetone, gel filtration chromatography on Sephadex G-100 and DEAE-cellulose chromatography, 47.14fold
-
TSC by ammonium sulfate fractionation, ion exchange chromatography, and gel filtration
Thermomonospora sp.
-
wild-type and mutants purified by immobilized metal affinity chromatography. Wild-type further purified by gel filtration
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
Escherichia coli
Escherichia coli and Streptomyces lividans, expression in Streptomyces lividans
expressed in Escherichia coli BL21(DE3) cells
expression in Escherichia coli
into vector pET30a and expressed in Escherichia coli BL21(DE3)
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RENATURED/Commentary
ORGANISM
UNIPROT
LITERATURE
denaturation studies using GdnCl indicate that TSC folds through a partially folded state that resembles molten globule at 1.8 M GdnCl. alpha-Crystallin chaperone-mediated in vitro folding, molecular mechanism, overview. Reconstitution of the active TSC is observed in 50 mM sodium phosphate buffer, pH 7.0, on cooling the alpha-crystallin-TSC-m complex to 4°C. Addition of alpha-crystallin to the molten globule-like intermediate of TSC complex initiates the refolding of TSC with 69% recovery of the biological activity of the enzyme
Thermomonospora sp.
-
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
analysis
-
tandem mass spectrometry to compare gas-phase and solution binding of small-molecule inhibitors to the wild type and three mutant forms of Cex
biofuel production
energy production
biofuel production
-
the enzyme can be used for hydrolysis of cellulosic and hemicellulosic biomass substrates for biofuel production
biofuel production
-
the enzyme can be used for hydrolysis of cellulosic and hemicellulosic biomass substrates for biofuel production
-
energy production
-
ethanol production from concentrated agricultural waste corncob
energy production
-
ethanol production from concentrated agricultural waste corncob
-
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Himmel, M.E.; Tucker, M.P.; Lastick, S.M.; Oh, K.K.; Fox, J.W.; Spindler, D.D.; Grohmann, K.
Isolation and characterization of a 1,4-beta-D-glucan glucohydrolase from the yeast, Torulopsis wickerhamii
J. Biol. Chem.
261
12948-12955
1986
Nakazawaea wickerhamii
brenda
Ohmiya, K.; Shimizu, S.
beta-Glucosidase from Ruminococcus albus
Methods Enzymol.
160
408-414
1988
Ruminococcus albus
-
brenda
Rao, M.; Mishra, C.
Glucohydrolase from Penicillium funiculosum
Appl. Microbiol. Biotechnol.
30
130-134
1989
Talaromyces funiculosus
-
brenda
Schmid, G.; Wandrey, C.
Characterization of a cellodextrin glucohydrolase with soluble oligomeric substrates: Experimental results and modeling of concentration-time-course data
Biotechnol. Bioeng.
33
1445-1460
1989
Trichoderma reesei, Trichoderma reesei QM 9414
brenda
Augustin, J.; Zemek, J.; Kuniak, B.; Fassatiova, O.
Production of beta-glucosidase, exo-beta-1,4-glucanase and endo-beta-1,4-glucanase by selected microscopic fungi
Folia Microbiol. (Praha)
26
14-18
1981
Botrytis cinerea, Pseudogymnoascus pannorum, Cladosporium cladosporioides, Amorphotheca resinae, Fusarium culmorum, Fusarium equiseti, Fusarium oxysporum, Fusarium oxysporum f. sp. dianthi, Fusarium sp., Fusarium solani, Gilmaniella humicola, Myrothecium striatisporum, Phoma sp., Rhizopus arrhizus, Scopulariopsis brevicaulis, Stachybotrys chartarum, Stemphylium botryosum, Thamnidium elegans, Berkeleyomyces basicola, Trichoderma koningii, Trichoderma viride, Trichoderma polysporum, Gibellulopsis nigrescens
brenda
Eriksson, K.E.; Petterson, B.
Extracellular enzyme system utilized by the fungus Sporotrichum pulverulentum (Chrysosporium lignorum) for the breakdown of cellulose. 3. Purification and physico-chemical characterization of an exo-1,4-beta-glucanase
Eur. J. Biochem.
51
213-218
1975
Sporotrichum pulverulentum
brenda
Wood, T.M.; McCrae, S.I.
Purification and some properties of a (1-4)-beta-D-glucan glucohydrolase associated with the cellulase from the fungus Penicillium funiculosum
Carbohydr. Res.
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291-303
1982
Talaromyces funiculosus
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brenda
Tahara, N.; Tonouchi, N.; Yano, H.; Yoshinaga, F.
Purification an characterization of exo-1,4-beta-glucosidase from Acetobacter xylinum BPR2001
J. Ferment. Bioeng.
85
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1998
Komagataeibacter xylinus, Komagataeibacter xylinus BPR2001
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brenda
Hamada, N.; Ishikawa, K.; Fuse, N.; Kodaira, R.; Shimosaka, M.; Amano, Y.; Kanda, T.; Okazaki, M.
Purification, characterization and gene analysis of exo-cellulase II (Ex-2) from the white rot basidiomycete Irpex lacteus
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Irpex lacteus
brenda
Bedarkar, S.; Gilkes, N.R.; Kilburn, D.G.; Kwan, E.; Rose, D.R.; Miller Jr., R.C.; Warren, R.A.J.; Withers, S.G.
Crystallization and preliminary X-ray diffraction analysis of the catalytic domain fo Cex, an exo-beta-1,4-glucanase and beta-1,4-xylanase from the bacterium Cellulomonas fimi
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Cellulomonas fimi
brenda
Doerner, K.C.; Gardner, R.M.; Schook, L.B.; Mackie, R.I.; White, B.A.
Inhibition of the exo-beta-1,4-glucanase from Ruminococcus flavefaciens FD-1 by a specific monoclonal antibody
Enzyme Microb. Technol.
16
2-9
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Ruminococcus flavefaciens, Ruminococcus flavefaciens FD-1
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brenda
Zverlov, V.; Mahr, S.; Riedel, K.; Bronnenmeier, K.
Properties and gene structure of a bifunctional celluloytic enzyme (CelA) from the extreme thermophile 'Anaerocellum thermophilum' with separate glycosyl hydrolase family 9 and 48 catalytic domains
Microbiology
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Caldicellulosiruptor bescii
brenda
Zhang, S.; Irwin, D.C.; Wilson, D.B.
Site-directed mutation of noncatalytic residues of Thermobifida fusca exocellulase Cel6B
Eur. J. Biochem.
267
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Thermobifida fusca
brenda
Hrmova, M.; MacGregor, E.A.; Biely, P.; Stewart, R.J.; Fincher, G.B.
Substrate binding and catalytic mechanism of a barley beta-D-glucosidase/(1,4)-beta-D-glucan exohydrolase
J. Biol. Chem.
273
11134-11143
1998
Hordeum vulgare
brenda
Rixon, J.E.; Ferreira, L.M.A.; Durrant, A.J.; Laurie, J.I.; Hazlewood, G.O.; Gilbert, H.J.
Characterization of the gene celD and its encoded product 1,4-beta-D-glucan glucohydrolase D from Pseudomonas fluorescens subsp. cellulosa
Biochem. J.
285
947-955
1992
Pseudomonas fluorescens
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brenda
Sharmila, T.; Sreeramulu, G.; Nand, K.
Purification and characterization of beta-1,4-glucosidase from Clostridium papyrosolvens
Biotechnol. Appl. Biochem.
27
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1998
Ruminiclostridium papyrosolvens
brenda
Bronnenmeier, K.; Kern, A.; Liebl, W.; Staudenbauer, W.L.
Purification of Thermotoga maritima enzymes for the degradation of cellulosic materials
Appl. Environ. Microbiol.
61
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1995
Thermotoga maritima
brenda
Ware, C.E.; Lachke, A.H.; Gregg, K.
Mode of action and substrate specificity of a purified exo-1,4-beta-D-glucosidase cloned from the cellulolytic bacterium Ruminococcus albus AR67
Biochem. Biophys. Res. Commun.
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Ruminococcus albus, Ruminococcus albus AR67
brenda
Ohmiya, K.; Shimizu, S.
Cellobiosidase from Ruminococcus albus
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Ruminococcus albus
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brenda
Gilkes, N.R.; Warren, A.J.; Miller Jr., R.C.; Kilburn, D.G.
Precise excision of the cellulose binding domains from two Cellulomonas fimi cellulases by a homologous protease an the effect on catalysis
J. Biol. Chem.
263
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Cellulomonas fimi
brenda
Wulff-Strobel, C.R.; Wilson, D.B.
Cloning, sequencing, and characterization of a membrane-associated Prevotella ruminicola B14 beta-glucosidase with cellodextrinase and cyanoglycosidase activities
J. Bacteriol.
177
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Prevotella ruminicola
brenda
Bronnenmeier, K.; Rucknagel, K.P.; Staudenbauer, W.L.
Purification and properties of a novel type of exo-1,4-beta-glucanase (avicelase II) from the cellulolytic thermophile Clostridium stercorarium
Eur. J. Biochem.
200
379-385
1991
Thermoclostridium stercorarium
brenda
El-Gindy, A.A.; Saad, R.R.; Fawzi, E.
Purification and some properties of exo-1,4-beta-glucanase from Chaetomium olivaceum
Acta Microbiol. Pol.
52
35-44
2003
Chaetomium globosum
brenda
Schubot, F.D.; Kataeva, I.A.; Chang, J.; Shah, A.K.; Ljungdahl, L.G.; Rose, J.P.; Wang, B.C.
Structural Basis for the exocellulase activity of the cellobiohydrolase CbhA from Clostridium thermocellum
Biochemistry
43
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Acetivibrio thermocellus
brenda
Valino, E.; Medina, I.; Dustet, J.
Characterization and optimization of the exo beta 1-4 glucanase enzyme of the Aspergillus fumigatus 155 v cellulolytic fungus strain
Cuban J. Agric. Sci.
34
227-236
2000
Aspergillus fumigatus, Aspergillus fumigatus 155 v
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brenda
Bravo, V.; Paez, M.P.; Aoulad, M.; Reyes, A.
The influence of temperature upon the hydrolysis of cellobiose by beta-1,4-glucosidases from Aspergillus niger
Enzyme Microb. Technol.
26
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2000
Aspergillus niger
brenda
Irwin, D.C.; Zhang, S.; Wilson, D.B.
Cloning, expression and characterization of a family 48 exocellulase, Cel48A, from Thermobifida fusca
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267
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Thermobifida fusca (Q9XCD4), Thermobifida fusca
brenda
Park, J.K.; Wang, L.X.; Patel, H.V.; Roseman, S.
Molecular cloning and characterization of a unique beta-glucosidase from Vibrio cholerae
J. Biol. Chem.
277
29555-29560
2002
Vibrio cholerae serotype O1, Vibrio cholerae serotype O1 E1 Tor N16961
brenda
Hamada, N.; Kodaira, R.; Nogawa, M.; Shinji, K.; Ito, R.; Amano, Y.; Shimosaka, M.; Kanda, T.; Okazaki, M.
Role of cellulose-binding domain of exocellulase I from white rot basidiomycete Irpex lacteus
J. Biosci. Bioeng.
91
359-362
2001
Irpex lacteus
brenda
Jagtap, S.; Rao, M.
Purification and properties of a low molecular weight 1,4-beta-d-glucan glucohydrolase having one active site for carboxymethyl cellulose and xylan from an alkalothermophilic Thermomonospora sp
Biochem. Biophys. Res. Commun.
329
111-116
2005
Thermomonospora sp.
brenda
Kosugi, A.; Arai, T.; Doi, R.H.
Degradation of cellulosome-produced cello-oligosaccharides by an extracellular non-cellulosomal beta-glucan glucohydrolase, BglA, from Clostridium cellulovorans
Biochem. Biophys. Res. Commun.
349
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2006
Clostridium cellulovorans (Q53EH2), Clostridium cellulovorans, Clostridium cellulovorans ATCC 35296 (Q53EH2)
brenda
Chen, M.; Xia, L.; Xue, P.
Enzymatic hydrolysis of corncob and ethanol production from cellulosic hydrolysate
Int. Biodeter. Biodegrad.
59
85-89
2007
Trichoderma reesei, Trichoderma reesei ZU-02
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brenda
Tesic, M.; Wicki, J.; Poon, D.K.; Withers, S.G.; Douglas, D.J.
Gas phase noncovalent protein complexes that retain solution binding properties: Binding of xylobiose inhibitors to the beta-1, 4 exoglucanase from Cellulomonas fimi
J. Am. Soc. Mass Spectrom.
18
64-73
2007
Cellulomonas fimi
brenda
Qi, M.; Jun, H.S.; Forsberg, C.W.
Cel9D, an atypical 1,4-beta-D-glucan glucohydrolase from Fibrobacter succinogenes: characteristics, catalytic residues, and synergistic interactions with other cellulases
J. Bacteriol.
190
1976-1984
2008
Fibrobacter succinogenes subsp. succinogenes S85 (B0FWI3)
brenda
Jagtap, S.; Rao, M.
Fluorescence study on interactions of alpha-crystallin with the molten globule state of 1, 4-beta-D-glucan glucanohydrolase from Thermomonospora sp. induced by guanidine hydrochloride
J. Fluoresc.
19
967-973
2009
Thermomonospora sp.
brenda
Nguyen, N.P.; Lee, K.M.; Lee, K.M.; Kim, I.W.; Kim, Y.S.; Jeya, M.; Lee, J.K.
One-step purification and characterization of a beta-1,4-glucosidase from a newly isolated strain of Stereum hirsutum
Appl. Microbiol. Biotechnol.
87
2107-2116
2010
Stereum hirsutum, Stereum hirsutum SKU512
brenda
Chir, J.L.; Wan, C.F.; Chou, C.H.; Wu, A.T.
Hydrolysis of cellulose in synergistic mixtures of beta-glucosidase and endo/exocellulase Cel9A from Thermobifida fusca
Biotechnol. Lett.
33
777-782
2011
Thermobifida fusca
brenda
Allardyce, B.J.; Linton, S.M.; Saborowski, R.
The last piece in the cellulase puzzle: the characterisation of beta-glucosidase from the herbivorous gecarcinid land crab Gecarcoidea natalis
J. Exp. Biol.
213
2950-2957
2010
Gecarcoidea natalis
brenda
Bao, L.; Huang, Q.; Chang, L.; Zhou, J.; Lu, H.
Screening and characterization of a cellulase with endocellulase and exocellulase activity from yak rumen metagenome
J. Mol. Catal. B
73
104-110
2011
uncultured bacterium
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brenda
Bao, L.; Huang, Q.; Chang, L.; Sun, Q.; Zhou, J.; Lu, H.
Cloning and characterization of two beta-glucosidase/xylosidase enzymes from yak rumen metagenome
Appl. Biochem. Biotechnol.
166
72-86
2012
uncultured rumen bacterium (G3BJX3), uncultured rumen bacterium (G3BJX4)
brenda
Shrestha, P.; Ibanez, A.B.; Bauer, S.; Glassman, S.I.; Szaro, T.M.; Bruns, T.D.; Taylor, J,W.
Fungi isolated from Miscanthus and sugarcane: biomass conversion, fungal enzymes, and hydrolysis of plant cell wall polymers
Biotechnol. Biofuels
8
38
2015
Alternaria sp., Aspergillus sp., Aureobasidium sp., Chloridium sp., Phanerodontia chrysosporium, Cladosporium sp., Penicillium sp., Exophiala sp., Fusarium sp., Neurospora crassa, Phoma sp., Trichoderma reesei, Trichoderma sp., Postia placenta, Bipolaris sp., Epicoccum sp., Dothideomycetes sp., Microdochium sp., Nigrospora sp., Arthrinium sp., Cephalosporium sp., Sporothrix sp., Minimidochium sp. TMS-2011, Cordyceps sp.
brenda
Takashima, S.; Nakamura, A.; Masaki, H.; Uozumi, T.
Purification and characterization of cellulases from Humicola grisea
Biosci. Biotechnol. Biochem.
60
77-82
1996
[Humicola] grisea var. thermoidea, [Humicola] grisea var. thermoidea IFO 9854
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brenda
Abdella, A.; Mazeed, T.; El-Baz, A.; Yang, S.
Production of beta-glucosidase from wheat bran and glycerol by Aspergillus niger in stirred tank and rotating fibrous bed bioreactors
Process Biochem.
51
1331-1337
2016
Aspergillus niger, Aspergillus niger NRRL 3112
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brenda
Zeng, R.; Hu, Q.; Yin, X.Y.; Huang, H.; Yan, J.B.; Gong, Z.W.; Yang, Z.H.
Cloning a novel endo-1,4-beta-D-glucanase gene from Trichoderma virens and heterologous expression in E. coli
AMB Express
6
108
2018
Trichoderma virens (A0A288W730), Trichoderma virens ZY-01 (A0A288W730)
brenda
Sermsathanaswadi, J.; Baramee, S.; Tachaapaikoon, C.; Pason, P.; Ratanakhanokchai, K.; Kosugi, A.
The family 22 carbohydrate-binding module of bifunctional xylanase/beta-glucanase Xyn10E from Paenibacillus curdlanolyticus B-6 has an important role in lignocellulose degradation
Enzyme Microb. Technol.
96
75-84
2017
Paenibacillus curdlanolyticus, Paenibacillus curdlanolyticus B-6
brenda
Jeong, W.S.; Seo, D.H.; Jung, J.H.; Jung, D.H.; Lee, D.W.; Park, Y.S.; Park, C.
Enzymatic characteristics of a highly thermostable beta-(1-4)-glucanase from Fervidobacterium islandicum AW-1 (KCTC 4680)
J. Microbiol. Biotechnol.
27
271-276
2017
Fervidobacterium islandicum, Fervidobacterium islandicum AW-1
brenda
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