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Information on EC 3.4.15.6 - cyanophycinase
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EC Tree
3.4.15.6 cyanophycinaseSpecify your search results
Word Map
- 3.4.15.6
- dipeptides
- cyanobacteria
- synechocystis
- anabaena
- cpha
- alcaligenes
-
diazotrophic
-
serine-type
-
gxsxg
- eubacteria
-
arginine-agarose
- asp-arg
-
water-insoluble
-
heterocyst-forming
-
nitrogen-rich
-
non-cyanobacterial
- heterocysts
- polyamide
-
l-aspartic
- nutrition
- food industry
The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota
Synonyms
cyanophycinase, cgpase, cphb1, extracellular cgpase, cpheal, cphb2, 
more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
CGPase
CphB
CphB1
CphB2
CphB3
CphB5
CphB6
CphE
CphEal
CphI
cyanophycin granule polypeptidase
cyanophycinase
extracellular CGPase
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
[L-Asp(4-L-Arg)]n + H2O = [L-Asp(4-L-Arg)]n-1 + L-Asp(4-L-Arg)
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of peptide bond
hydrolysis of peptide bond
the enzyme seems to belong to the familiy of serine proteases
hydrolysis of peptide bond
-
the enzyme seems to belong to the familiy of serine proteases
-
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PATHWAY SOURCE
PATHWAYS
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-
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CAS REGISTRY NUMBER
COMMENTARY
131554-16-0
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
modified soluble cyanobacterial granule polypeptide + H2O
beta-aspartate-arginine + beta-aspartate-homoarginine + ?
modified soluble cyanobacterial granule polypeptide + H2O
beta-aspartate-arginine + beta-aspartate-homoarginine + ?
in vitro guanidination of lysine side chains of soluble cyanobacterial granule polypeptide with o-methylisourea yields the nonproteinogenic amino acid homoarginine. The modified soluble cyanobacterial granule polypeptide consists of 51 mol% aspartate, 14 mol% arginine, and 35 mol% homoarginine. The modified cyanobacterial granule polypeptide can be digested in vitro using cyanophycinase, yielding a new dipeptide consisting of aspartate and homoarginine
-
-
?
modified soluble cyanobacterial granule polypeptide + H2O
beta-aspartate-arginine + beta-aspartate-homoarginine + ?
in vitro guanidination of lysine side chains of soluble cyanobacterial granule polypeptide with o-methylisourea yields the nonproteinogenic amino acid homoarginine. The modified soluble cyanobacterial granule polypeptide consists of 51 mol% aspartate, 14 mol% arginine, and 35 mol% homoarginine. The modified cyanobacterial granule polypeptide can be digested in vitro using cyanophycinase, yielding a new dipeptide consisting of aspartate and homoarginine
-
-
?
[L-Asp(4-Arg)]n + H2O
[L-Asp(4-Arg)]n-1 + [L-Asp(4-Arg)]
-
2 g/l citrate, pH 6.5, and 37°C are ideal parameters for cyanophycinase production
-
-
?
[L-Asp(4-Arg)]n + H2O
[L-Asp(4-Arg)]n-1 + [L-Asp(4-Arg)]
-
-
-
-
?
[L-Asp(4-Arg)]n + H2O
[L-Asp(4-Arg)]n-1 + [L-Asp(4-Arg)]
-
2 g/l citrate, pH 6.5, and 37°C are ideal parameters for cyanophycinase production
-
-
?
[L-Asp(4-Arg)]n + H2O
[L-Asp(4-Arg)]n-1 + [L-Asp(4-Arg)]
-
-
-
?
[L-Asp(4-Arg)]n + H2O
[L-Asp(4-Arg)]n-1 + [L-Asp(4-Arg)]
-
-
-
?
[L-Asp(4-Arg)]n + H2O
[L-Asp(4-Arg)]n-1 + [L-Asp(4-Arg)]
-
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
-
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
-
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
-
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
-
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
-
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
-
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
-
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
-
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
-
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
-
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
-
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
-
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
-
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
-
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
-
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
-
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
-
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
-
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
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also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
-
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
-
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
-
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
-
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
-
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
-
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
-
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
-
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
-
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
-
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
-
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
-
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
-
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
-
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
-
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
-
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
Thermosynechococcus vestitus
-
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
-
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
[L-Asp(4-Arg)]n + H2O
[L-Asp(4-Arg)]n-1 + [L-Asp(4-Arg)]
-
-
-
-
?
[L-Asp(4-Arg)]n + H2O
[L-Asp(4-Arg)]n-1 + [L-Asp(4-Arg)]
-
-
-
?
[L-Asp(4-Arg)]n + H2O
[L-Asp(4-Arg)]n-1 + [L-Asp(4-Arg)]
-
-
-
?
[L-Asp(4-Arg)]n + H2O
[L-Asp(4-Arg)]n-1 + [L-Asp(4-Arg)]
-
-
-
?
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
3,4-dichloro-isocoumarin
78% inhibition at 1 mM, 56% inhibition at 0.1 mM
4-(2-aminoethyl) benzenesulfonylfluoride
-
cyanophycinase is strongly inhibited
leupeptin
13% inhibition within the range 0.001 nM to 0.01 nM
N-bromo-succinimide
100% inhibition at 5 nM, 99% inhibition at 1 nM
phenylmethanesulfonyl fluoride
45% inhibition at 10 mM, 8% inhibition at 1 mM
pefabloc
i.e. 4-[2-aminoethyl]benzenesulfonyl fluoride, 92% inhibition at 80 nM, 13.6% inhibition at 8 nM
pefabloc
i.e. 4-[2-aminoethyl]benzenesulfonyl fluoride, 79% inhibition at 20 mM, 23% inhibition at 8 mM
phenylmethylsulfonyl fluoride
63% inhibition at 10 nM, 28% inhibition at 1 nM
additional information
-
no inhibition by EDTA; no inhibition by leupeptin
-
additional information
not inhibitory: pepstatin, diazoacetyl-DL-nor-leucine-methyl ester, aprotinin
-
additional information
-
not inhibitory: pepstatin, diazoacetyl-DL-nor-leucine-methyl ester, aprotinin
-
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0022
[L-Asp(4-Arg)]n
30°C in 50 mM N-[2-hydroxy-1,1-bis(hydroxymethyl) ethyl]glycine, pH 8.0
-
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
16.5
[L-Asp(4-Arg)]n
30°C in 50 mM N-[2-hydroxy-1,1-bis(hydroxymethyl) ethyl]glycine, pH 8.0, maximum at pH 9.0
-
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
7500
[L-Asp(4-Arg)]n
30°C in 50 mM N-[2-hydroxy-1,1-bis(hydroxymethyl) ethyl]glycine, maximum at pH 8.0
-
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Thermosynechococcus vestitus
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
additional information
cyanophycinase is amplified from Synechocystis sp. PCC 6803 genomic DNA using iProof polymerase and is ligated into the BamHI and NdeI sites of pET-28a, plasmids are transformed into Rosetta cells
brenda
cells grown in medium containing cyanophycin granules
brenda
-
cells grown in medium containing cyanophycin granules
-
brenda
enzyme is detected only in germinated spore membranes
brenda
-
enzyme is detected only in germinated spore membranes
-
brenda
-
compartmentalized degradation of cyanophycin, with the first step, catalyzed by cyanophycinase, taking place in the heterocysts and the second step, catalyzed by a dipeptidase, taking place mainly in the vegetative cells
brenda
-
compartmentalized degradation of cyanophycin, with the first step, catalyzed by cyanophycinase, taking place in the heterocysts and the second step, catalyzed by a dipeptidase, taking place mainly in the vegetative cells
-
brenda
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE