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4-[[4-(dimethylamino)phenyl]azo]benzoic acid-MYNKMGGGDKTVSF-(5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid) amide + H2O
?
coronavirus nonstructural protein 1 + H2O
?
-
Substrates: -
Products: -
?
coronavirus nonstructural protein 2 + H2O
?
-
Substrates: -
Products: -
?
coronavirus nonstructural protein 3 + H2O
?
-
Substrates: -
Products: -
?
ISG15-7-amido-4-methylcoumarin + H2O
ISG15 + 7-amino-4-methylcoumarin
KAGG-7-amido-4-methylcoumarin + H2O
KAGG + 7-amino-4-methylcoumarin
KKAG-7-amido-4-methylcoumarin + H2O
KKAG + 7-amino-4-methylcoumarin
L-Arg-L-Leu-L-Arg-Gly-Gly-7-amido-4-methylcoumarin + H2O
L-Arg-L-Leu-L-Arg-Gly-Gly + 7-amino-4-methylcoumarin
-
Substrates: -
Products: -
?
LRGG-7-amido-4-methylcoumarin + H2O
LRGG + 7-amino-4-methylcoumarin
Lys63-Ub4 + H2O
?
Substrates: -
Products: -
?
ORF 1a polyprotein + H2O
?
ORF 1a polyprotein + H2O
nsp1 + nsp2 + nsp3
p28
?
-
Substrates: -
Products: -
?
p65
?
-
Substrates: -
Products: -
?
proISG + H2O
?
Substrates: i.e. proenzyme of interferon-stimulated gene product 15
Products: -
?
tetra-ubiquitin + H2O
2 di-ubiquitin
-
Substrates: -
Products: -
?
ubiquitin-7-amido-4-methylcoumarin + H2O
ubiquitin + 7-amino-4-methylcoumarin
ubiquitinated RIG-I + H2O
ubiquitin + RIG-I
-
Substrates: substrate is a key fegulator in the IFN signaling pathway
Products: -
?
ubiquitinated STING + H2O
ubiquitin + STINGI
-
Substrates: substrate is a key fegulator in the IFN signaling pathway
Products: -
?
additional information
?
-
4-[[4-(dimethylamino)phenyl]azo]benzoic acid-MYNKMGGGDKTVSF-(5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid) amide + H2O
?
Substrates: -
Products: -
?
4-[[4-(dimethylamino)phenyl]azo]benzoic acid-MYNKMGGGDKTVSF-(5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid) amide + H2O
?
Substrates: -
Products: -
?
ISG15-7-amido-4-methylcoumarin + H2O
ISG15 + 7-amino-4-methylcoumarin
-
Substrates: -
Products: -
?
ISG15-7-amido-4-methylcoumarin + H2O
ISG15 + 7-amino-4-methylcoumarin
-
Substrates: -
Products: -
?
KAGG-7-amido-4-methylcoumarin + H2O
KAGG + 7-amino-4-methylcoumarin
Substrates: -
Products: -
?
KAGG-7-amido-4-methylcoumarin + H2O
KAGG + 7-amino-4-methylcoumarin
Substrates: -
Products: -
?
KKAG-7-amido-4-methylcoumarin + H2O
KKAG + 7-amino-4-methylcoumarin
Substrates: -
Products: -
?
KKAG-7-amido-4-methylcoumarin + H2O
KKAG + 7-amino-4-methylcoumarin
Substrates: -
Products: -
?
LRGG-7-amido-4-methylcoumarin + H2O
LRGG + 7-amino-4-methylcoumarin
Substrates: -
Products: -
?
LRGG-7-amido-4-methylcoumarin + H2O
LRGG + 7-amino-4-methylcoumarin
Substrates: -
Products: -
?
Lys48-Ub4 + H2O
?
Substrates: -
Products: -
?
Lys48-Ub4 + H2O
?
Substrates: -
Products: -
?
ORF 1a polyprotein + H2O
?
Substrates: -
Products: -
?
ORF 1a polyprotein + H2O
?
Substrates: cleavage in cis and in trans
Products: -
?
ORF 1a polyprotein + H2O
?
Substrates: cleavage sites are: Gly247-/-Val248 and Gly821-/-Val822. PLP-1 is responsible for the cleavage of the amino-terminal protein p28
Products: -
?
ORF 1a polyprotein + H2O
?
Substrates: cleavage in cis and in trans. PLP-1 cleavage activity appears to be optimal when both enzyme and substrate are present as part of polyproteins, as they are in the virus replication cycle
Products: -
?
ORF 1a polyprotein + H2O
?
Substrates: the enzyme is involved in processing of ORF 1a polyprotein into mature replicase-related polypeptides
Products: -
?
ORF 1a polyprotein + H2O
?
Substrates: -
Products: -
?
ORF 1a polyprotein + H2O
?
Substrates: cleavage in cis and in trans
Products: -
?
ORF 1a polyprotein + H2O
?
Substrates: cleavage in cis and in trans. PLP-1 cleavage activity appears to be optimal when both enzyme and substrate are present as part of polyproteins, as they are in the virus replication cycle
Products: -
?
ORF 1a polyprotein + H2O
?
Substrates: cleavage sites are: Gly247-/-Val248 and Gly821-/-Val822. PLP-1 is responsible for the cleavage of the amino-terminal protein p28
Products: -
?
ORF 1a polyprotein + H2O
?
Substrates: the enzyme is involved in processing of ORF 1a polyprotein into mature replicase-related polypeptides
Products: -
?
ORF 1a polyprotein + H2O
?
Substrates: -
Products: -
?
ORF 1a polyprotein + H2O
?
Substrates: -
Products: -
?
ORF 1a polyprotein + H2O
nsp1 + nsp2 + nsp3
-
Substrates: SARS coronavirus replicase polyproteins pp1a and pp1ab are predicted to be processed into 16 nonstructural proteins, NSP2-3 intermediate identified
Products: identified products
?
ORF 1a polyprotein + H2O
nsp1 + nsp2 + nsp3
-
Substrates: SARS coronavirus replicase polyproteins pp1a and pp1ab are predicted to be processed into 16 nonstructural proteins, NSP2-3 intermediate identified
Products: identified products
?
ubiquitin-7-amido-4-methylcoumarin + H2O
ubiquitin + 7-amino-4-methylcoumarin
-
Substrates: -
Products: -
?
ubiquitin-7-amido-4-methylcoumarin + H2O
ubiquitin + 7-amino-4-methylcoumarin
-
Substrates: -
Products: -
?
ubiquitin-7-amido-4-methylcoumarin + H2O
ubiquitin + 7-amino-4-methylcoumarin
Substrates: -
Products: -
?
ubiquitin-7-amido-4-methylcoumarin + H2O
ubiquitin + 7-amino-4-methylcoumarin
Substrates: -
Products: -
?
additional information
?
-
Substrates: PLpro can degrade Lys48- and Lys63-linked polyubiquitin chains to monoubiquitin but not linear polyubiquitin
Products: -
?
additional information
?
-
Substrates: PLpro can degrade Lys48- and Lys63-linked polyubiquitin chains to monoubiquitin but not linear polyubiquitin
Products: -
?
additional information
?
-
-
Substrates: enzyme is able to cleave ubiquitin mutant L73P in K6-, K11-, K48- and K63-linked di-ubiquitin chains. Cleavage rates with MERS PLpro are nearly identical against ubiquitin chains of various lengths
Products: -
?
additional information
?
-
-
Substrates: MERS PLpro has an absolute requirement for Gly in the P2 position, but can accommodate a wide variety of hydrophobic amino acids in both the P3 and P4 positions. The most preferred amino acid in P3 is Leu, followed by Arg, Lys and hydrophobic residues. The most preferred amino acid in P4 is also Leu, followed by mostly hydrophobic residues
Products: -
?
additional information
?
-
Substrates: viral substrates translated from pSPDELTAMscN1S1, p28, p43 and p70, efficient cleavage requires a substrate greater than 69000 Da
Products: -
?
additional information
?
-
-
Substrates: viral substrates translated from pSPDELTAMscN1S1, p28, p43 and p70, efficient cleavage requires a substrate greater than 69000 Da
Products: -
?
additional information
?
-
Substrates: the virus can regulate in vivo proteinase activity through self inactivation of PLP-1. The lack of p50 production in cells infected with the mouse hepatitis virus strain JHM may regulate PLP-1 activity differently from MHV-59
Products: -
?
additional information
?
-
Substrates: the virus can regulate in vivo proteinase activity through self inactivation of PLP-1. The lack of p50 production in cells infected with the mouse hepatitis virus strain JHM may regulate PLP-1 activity differently from MHV-59
Products: -
?
additional information
?
-
Substrates: viral substrates translated from pSPDELTAMscN1S1, p28, p43 and p70, efficient cleavage requires a substrate greater than 69000 Da
Products: -
?
additional information
?
-
-
Substrates: interactions of PLP1 with CS1 and CS2 are critical for protein processing and suggest that the interactions play specific roles in regulation of the functions of nsp1, 2, and 3 in viral RNA synthesis
Products: -
?
additional information
?
-
-
Substrates: SARS PLpro recognizes a K48-linked tri-Ub as a minimal unit for ubiquitin chain processing
Products: -
?
additional information
?
-
Substrates: PL1pro cleaves a peptide mimicking the cognate nsp2-nsp3 cleavage site, has deubiquitinating activity in an in vitro cleavage assay and displays a slight preference for Lys48-linked polyubiquitin chains over Lys63-linked ones
Products: -
?
additional information
?
-
Substrates: PL1pro cleaves a peptide mimicking the cognate nsp2-nsp3 cleavage site, has deubiquitinating activity in an in vitro cleavage assay and displays a slight preference for Lys48-linked polyubiquitin chains over Lys63-linked ones
Products: -
?
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0.0121
2,6-dimethyl-N-[(R)-1-(2-naphthyl)ethyl]benzamide
Severe acute respiratory syndrome-related coronavirus
-
in 50 mM HEPES, pH 7.5
0.0226
2,N-dimethyl-N-[(R)-1-(2-naphthyl)ethyl]benzamide
Severe acute respiratory syndrome-related coronavirus
-
in 50 mM HEPES, pH 7.5
0.09
2-methoxy-N-[(R)-1-(2-naphthyl)ethyl]benzamide
Severe acute respiratory syndrome-related coronavirus
-
in 50 mM HEPES, pH 7.5
0.0014
2-methoxymethyl-5-nitrobenzoic acid methyl ester
Severe acute respiratory syndrome-related coronavirus
-
in 50 mM HEPES, pH 7.5
0.0023
2-methyl-N-[(R)-1-(1-naphthyl)ethyl]benzamide
Severe acute respiratory syndrome-related coronavirus
-
in 50 mM HEPES, pH 7.5
0.0087
2-methyl-N-[1-(naphthalen-2-yl)ethyl]benzamide
Severe acute respiratory syndrome-related coronavirus
-
in 50 mM HEPES, pH 7.5
0.0135
3-methoxy-N-[(R)-1-(2-naphthyl)ethyl]benzamide
Severe acute respiratory syndrome-related coronavirus
-
in 50 mM HEPES, pH 7.5
0.0148
3-methyl-N-[(R)-1-(2-naphthyl)ethyl]benzamide
Severe acute respiratory syndrome-related coronavirus
-
in 50 mM HEPES, pH 7.5
0.0248
4-amino-N-[(R)-1-(1-naphthyl)ethyl]benzamide
Severe acute respiratory syndrome-related coronavirus
-
in 50 mM HEPES, pH 7.5
0.0461
4-amino-N-[(R)-1-(2-naphthyl)ethyl]benzamide
Severe acute respiratory syndrome-related coronavirus
-
in 50 mM HEPES, pH 7.5
0.149
4-methoxy-N-[(R)-1-(2-naphthyl)ethyl]benzamide
Severe acute respiratory syndrome-related coronavirus
-
in 50 mM HEPES, pH 7.5
0.0291
4-methyl-N-[(R)-1-(2-naphthyl)ethyl]benzamide
Severe acute respiratory syndrome-related coronavirus
-
in 50 mM HEPES, pH 7.5
0.0052
5-amino-2-methoxymethyl-N-[1-methyl-1-(1-naphthyl)ethyl]benzamide
Severe acute respiratory syndrome-related coronavirus
-
in 50 mM HEPES, pH 7.5
0.00056
5-amino-2-methyl-N-[(R)-1-(1-naphthyl)ethyl]benzamide
Severe acute respiratory syndrome-related coronavirus
-
in 50 mM HEPES, pH 7.5
0.00056
5-amino-2-methyl-N-[1-methyl-1-(1-naphthyl)ethyl]benzamide
Severe acute respiratory syndrome-related coronavirus
-
in 50 mM HEPES, pH 7.5
0.0111
5-cyano-2-methyl-N-[1-methyl-1-(1-naphthyl)ethyl]benzamide
Severe acute respiratory syndrome-related coronavirus
-
in 50 mM HEPES, pH 7.5
0.0027
5-iodo-2-methyl-N-[1-methyl-1-(1-naphthyl)ethyl]benzamide
Severe acute respiratory syndrome-related coronavirus
-
in 50 mM HEPES, pH 7.5
0.00046
5-methylamino-2-methyl-N-[(R)-1-(1-naphthyl)ethyl]benzamide
Severe acute respiratory syndrome-related coronavirus
-
in 50 mM HEPES, pH 7.5
0.00264
5-N-acetylamino-2-methyl-N-[(R)-1-(1-naphthyl)ethyl]benzamide
Severe acute respiratory syndrome-related coronavirus
-
in 50 mM HEPES, pH 7.5
0.0048
5-N-tert-butoxycarbonylmethylamino-2-methyl-N'-[(R)-1-(1-naphthyl)ethyl]benzamide
Severe acute respiratory syndrome-related coronavirus
-
in 50 mM HEPES, pH 7.5
0.0013
N-methyl-5-methylamino-2-methyl-N'-[(R)-1-(1-naphthyl)ethyl]benzamide
Severe acute respiratory syndrome-related coronavirus
-
in 50 mM HEPES, pH 7.5
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Teng, H.; Pinon, J.D.; Weiss, S.R.
Expression of murine coronavirus recombinant papain-like proteinase: efficient cleavage is dependent on the lengths of both the substrate and the proteinase polypeptide
J. Virol.
73
2658-2666
1999
Murine coronavirus (P0C6V1), Murine coronavirus, Murine coronavirus JHM (P0C6V1)
brenda
Teng, H.; Weiss, S.R.
Further in vitro characterization of mouse hepatitis virus papain-like proteinase 1: cleavage sequence requirements within pp1a
J. Neurovirol.
8
143-149
2002
Murine coronavirus (P0C6V1), Murine coronavirus JHM (P0C6V1)
brenda
Bonilla, P.J.; Hughes, S.A.; Pinon, J.D.; Weiss, S.R.
Characterization of the leader papain-like proteinase of MHV-A59: identification of a new in vitro cleavage site
Virology
209
489-497
1995
Murine coronavirus (P0C6V1), Murine coronavirus JHM (P0C6V1)
brenda
Kanjanahaluethai, A.; Baker, S.C.
Coronavirus papain-like endopeptidases
Handbook of Proteolytic Enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , eds. )Academic Press
2
1265-1269
2004
Murine hepatitis virus
-
brenda
Harcourt, B.H.; Jukneliene, D.; Kanjanahaluethai, A.; Bechill, J.; Severson, K.M.; Smith, C.M.; Rota, P.A.; Baker, S.C.
Identification of severe acute respiratory syndrome coronavirus replicase products and characterization of papain-like protease activity
J. Virol.
78
13600-13612
2004
Severe acute respiratory syndrome-related coronavirus, Severe acute respiratory syndrome-related coronavirus Urbani
brenda
Sulea, T.; Lindner, H.A.; Purisima, E.O.; Mnard, R.
Binding site-based classification of coronaviral papain-like proteases
Proteins Struct. Funct. Bioinform.
62
760-775
2006
bovine coronavirus (P0C6W7), bovine coronavirus Ent (P0C6W7), Human coronavirus 229E (P0C6X1), infectious bronchitis virus (P0C6V3), infectious bronchitis virus Beaudette (P0C6V3), Murine hepatitis virus (P0C6V0), Murine hepatitis virus A59 (P0C6V0), porcine epidemic diarrhea virus (P0C6Y4), Severe acute respiratory syndrome-related coronavirus (P0C6X7), Severe acute respiratory syndrome-related coronavirus, Transmissible gastroenteritis virus (P0C6V2)
brenda
Graham, R.L.; Denison, M.R.
Replication of murine hepatitis virus is regulated by papain-like proteinase 1 processing of nonstructural proteins 1, 2, and 3
J. Virol.
80
11610-11620
2006
Murine hepatitis virus
brenda
Ghosh, A.K.; Takayama, J.; Aubin, Y.; Ratia, K.; Chaudhuri, R.; Baez, Y.; Sleeman, K.; Coughlin, M.; Nichols, D.B.; Mulhearn, D.C.; Prabhakar, B.S.; Baker, S.C.; Johnson, M.E.; Mesecar, A.D.
Structure-based design, synthesis, and biological evaluation of a series of novel and reversible inhibitors for the severe acute respiratory syndrome-coronavirus papain-like protease
J. Med. Chem.
52
5228-5240
2009
Severe acute respiratory syndrome-related coronavirus
brenda
Wojdyla, J.A.; Manolaridis, I.; van Kasteren, P.B.; Kikkert, M.; Snijder, E.J.; Gorbalenya, A.E.; Tucker, P.A.
Papain-like protease 1 from transmissible gastroenteritis virus: Crystal structure and enzymatic activity toward viral and cellular substrates
J. Virol.
84
10063-10073
2010
Transmissible gastroenteritis virus (P0C6V2), Transmissible gastroenteritis virus Purdue (P0C6V2)
brenda
Gadlage, M.J.; Denison, M.R.
Exchange of the coronavirus replicase polyprotein cleavage sites alters protease specificity and processing
J. Virol.
84
6894-6898
2010
Murine hepatitis virus
brenda
Bekes, M.; Rut, W.; Kasperkiewicz, P.; Mulder, M.; Ovaa, H.; Drag, M.; Lima, C.; Huang, T.
SARS hCoV papain-like protease is a unique Lys48 linkage-specific di-distributive deubiquitinating enzyme
Biochem. J.
468
215-226
2015
Middle East respiratory syndrome-related coronavirus, Severe acute respiratory syndrome-related coronavirus
brenda
Hu, X.; Tian, J.; Kang, H.; Guo, D.; Liu, J.; Liu, D.; Jiang, Q.; Li, Z.; Qu, J.; Qu, L.
Transmissible gastroenteritis virus papain-like protease 1 antagonizes production of interferon-beta through its deubiquitinase activity
BioMed Res. Int.
2017
7089091
2017
Transmissible gastroenteritis virus
brenda
Kong, L.; Shaw, N.; Yan, L.; Lou, Z.; Rao, Z.
Structural view and substrate specificity of papain-like protease from avian infectious bronchitis virus
J. Biol. Chem.
290
7160-7168
2015
infectious bronchitis virus (P0C6Y1), infectious bronchitis virus Beaudette (P0C6Y1)
brenda
Daczkowski, C.M.; Dzimianski, J.V.; Clasman, J.R.; Goodwin, O.; Mesecar, A.D.; Pegan, S.D.
Structural insights into the interaction of coronavirus papain-like proteases and interferon-stimulated gene product 15 from different species
J. Mol. Biol.
429
1661-1683
2017
Severe acute respiratory syndrome-related coronavirus (P0C6X7)
brenda
Niemeyer, D.; Moesbauer, K.; Klein, E.; Sieberg, A.; Mettelman, R.; Mielech, A.; Dijkman, R.; Baker, S.; Drosten, C.; Mueller, M.
The papain-like protease determines a virulence trait that varies among members of the SARS-coronavirus species
PLoS Pathog.
14
e1007296
2018
SARS coronavirus FRA (AY310120)
brenda
Li, S.; Wang, C.; Jou, Y.; Yang, T.; Huang, S.; Wan, L.; Lin, Y.; Lin, C.
SARS coronavirus papain-like protease induces Egr-1-dependent up-regulation of TGF-beta via ROS/p38 MAPK/STAT3 pathway
Sci. Rep.
6
25754
2016
Severe acute respiratory syndrome-related coronavirus (P0C6X7)
brenda