the enzyme shows preference for Phe and His over Leu, Ile, Val, Arg, and Lys. Peptides with positively charged amino acids Arg and Lys or aliphatic amino acids Leu, Ile, and Val are all poor substrates, 2-aminobenzoyl-KLIPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine], 2-aminobenzoyl-KLWPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine], 2-aminobenzoyl-KLVSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine], 2-aminobenzoyl-KLISSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine], 2-aminobenzoyl-KLWSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine], and 2-aminobenzoyl-KLPSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] are resistant to hydrolysis

709118

additional information

Scytalidium lignicola

P15369

the enzyme has a narrow substrate specificity, with preference for small, basic residues

753080

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NATURAL SUBSTRATE

NATURAL PRODUCT

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

REVERSIBILITY
r=reversible
ir=irreversible
?=not specified

additional information

Scytalidium lignicola

the enzyme is involved in protein degradation in wood destroying by the fungus

668804

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METALS and IONS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

CuCl2

Scytalidium lignicola

10 mM, activates

30754

MnCl2

Scytalidium lignicola

10 mM, activates

30764

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INHIBITOR

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

1,2-epoxy-3-(4-nitrophenoxy)propane

5 entries

1-diazo-3-phenyl-2-propanone

Scytalidium lignicola

modifies active site residue Asp98

668804

Ac-FKF-(3S,4S)-phenylstatinyl-LR-NH

Scytalidium lignicola

685437

acetyl-Phe--Lys-Phe-(3S,4S)-phenylstatinyl-Leu-Arg-NH2

Scytalidium lignicola

SGP transition state mimic inhibitor

709118

N-bromosuccinimide

Scytalidium lignicola

30754

NaCl

Scytalidium lignicola

a substantial decrease of the SGP catalytic efficiency (kcat/Km) is observed as the NaCl concentration is increases up to 1 M, while in the range up to 200 mM NaCl the effect is mainly in Km because there is no significant decrease in kcat values. A significant decrease in the kcat values is observed as the salt concentration is further increased from 200 to 800 mM NaCl

709118

additional information

7 entries

1,2-epoxy-3-(4-nitrophenoxy)propane

Ganoderma lucidum

not

30767

1,2-epoxy-3-(4-nitrophenoxy)propane

Lentinula edodes

not

30766

1,2-epoxy-3-(4-nitrophenoxy)propane

Scytalidium lignicola

30754, 30762

1,2-epoxy-3-(4-nitrophenoxy)propane

Scytalidium lignicola

reacts with Glu53, which replaces one of the aspartic residues at the active centre

30765

1,2-epoxy-3-(4-nitrophenoxy)propane

Scytalidium lignicola

modifies active site residue Glu53

668804

additional information

Ganoderma lucidum

diazoacetyl-DL-norleucine methyl ester; methyl 2-diazoacetamidohexanoate; not: pepstatin Ac; Streptomyces-pepsin inhibitor (S-PI)

30767

additional information

Lentinula edodes

diazoacetyl-DL-norleucine methyl ester; diisopropyl fluorophosphate; EDTA; methyl 2-diazoacetamidohexanoate; microbial proteinase inhibitors (metal proteinase inhibitor, serine proteinase and thiol proteinase inhibitor); not: pepstatin Ac; PCMB; PMSF; Streptomyces-pepsin inhibitor (S-PI)

30766

additional information

Scytalidium lignicola

diazoacetyl-DL-norleucine methyl ester; diisopropyl fluorophosphate; EDTA; methyl 2-diazoacetamidohexanoate; Streptomyces-pepsin inhibitor (S-PI); sulfhydryl reagents

30754

additional information

Scytalidium lignicola

methyl 2-diazoacetamidohexanoate; not: pepstatin

30759

additional information

Scytalidium lignicola

diazoacetyl-DL-norleucine methyl ester; methyl 2-diazoacetamidohexanoate; Streptomyces-pepsin inhibitor (S-PI)

30762

additional information

Scytalidium lignicola

methyl 2-diazoacetamidohexanoate; not: pepstatin

30765

additional information

Scytalidium lignicola

the enzyme is insensitive to pepstatin and inhibitor S-PI, i.e. Ac-VV-statine-A-statine, statine is 4-amino-3-hydroxy-6-methylheptanoic acid, no inhibition by diazoacetyl-DL-norleucine methyl ester

668804

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KM VALUE [mM]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.0022

2-aminobenzoyl-KLAPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

0.0023

2-aminobenzoyl-KLASSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

0.0039

2-aminobenzoyl-KLDPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

0.0046

2-aminobenzoyl-KLDSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

0.004

2-aminobenzoyl-KLESPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

0.0008

2-aminobenzoyl-KLESSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

0.0002

2-aminobenzoyl-KLFPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

0.0012

2-aminobenzoyl-KLFSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

0.002

2-aminobenzoyl-KLGPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

0.0046

2-aminobenzoyl-KLGSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

0.0001

2-aminobenzoyl-KLHPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

0.0004

2-aminobenzoyl-KLHSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

0.00055

2-aminobenzoyl-KLKPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

0.0005

2-aminobenzoyl-KLKSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

0.0016

2-aminobenzoyl-KLLSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

0.0008

2-aminobenzoyl-KLMSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

0.00026

2-aminobenzoyl-KLNPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

0.003

2-aminobenzoyl-KLNSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

0.0004

2-aminobenzoyl-KLPSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

0.0009

2-aminobenzoyl-KLQPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

0.0008

2-aminobenzoyl-KLQSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

0.0003

2-aminobenzoyl-KLRPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

0.0008

2-aminobenzoyl-KLRSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

0.0014

2-aminobenzoyl-KLSPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

0.0004

2-aminobenzoyl-KLSSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

0.0017

2-aminobenzoyl-KLVSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

0.00025

2-aminobenzoyl-KLYPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

0.0011

2-aminobenzoyl-KLYSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

0.000065

D-2,3-diaminopropionic acid(N-methylanthraniloyl)-GFKFFALRK(2,4-dinitrophenol)

Scytalidium lignicola

685437

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TURNOVER NUMBER [1/s]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

1.6

2-aminobenzoyl-KLAPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

2-aminobenzoyl-KLASSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

0.6

2-aminobenzoyl-KLDPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

2-aminobenzoyl-KLDSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

0.4

2-aminobenzoyl-KLESPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

0.2

2-aminobenzoyl-KLESSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

2-aminobenzoyl-KLFPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

200

2-aminobenzoyl-KLFSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

0.4

2-aminobenzoyl-KLGPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

3.6

2-aminobenzoyl-KLGSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

2-aminobenzoyl-KLHPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

145

2-aminobenzoyl-KLHSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

3.5

2-aminobenzoyl-KLKPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

2-aminobenzoyl-KLKSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

7.7

2-aminobenzoyl-KLLSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

105

2-aminobenzoyl-KLMSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

6.5

2-aminobenzoyl-KLNPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

198

2-aminobenzoyl-KLNSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

7.7

2-aminobenzoyl-KLPSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

0.4

2-aminobenzoyl-KLQPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

0.8

2-aminobenzoyl-KLQSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

1.1

2-aminobenzoyl-KLRPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

2-aminobenzoyl-KLRSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

0.03

2-aminobenzoyl-KLSPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

0.063

2-aminobenzoyl-KLSSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

0.048

2-aminobenzoyl-KLVSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

8.7

2-aminobenzoyl-KLYPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

2-aminobenzoyl-KLYSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

34.8

D-2,3-diaminopropionic acid(N-methylanthraniloyl)-GFKFFALRK(2,4-dinitrophenol)

Scytalidium lignicola

685437

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kcat/KM VALUE [1/mMs^-1]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

700

2-aminobenzoyl-KLAPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

2600

2-aminobenzoyl-KLASSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

150

2-aminobenzoyl-KLDPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

3700

2-aminobenzoyl-KLDSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

100

2-aminobenzoyl-KLESPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

250

2-aminobenzoyl-KLESSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

65000

2-aminobenzoyl-KLFPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

167000

2-aminobenzoyl-KLFSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

200

2-aminobenzoyl-KLGPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

800

2-aminobenzoyl-KLGSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

330000

2-aminobenzoyl-KLHPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

363000

2-aminobenzoyl-KLHSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

6400

2-aminobenzoyl-KLKPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

16000

2-aminobenzoyl-KLKSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

4800

2-aminobenzoyl-KLLSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

131000

2-aminobenzoyl-KLMSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

26000

2-aminobenzoyl-KLNPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

66000

2-aminobenzoyl-KLNSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

19000

2-aminobenzoyl-KLPSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

400

2-aminobenzoyl-KLQPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

1000

2-aminobenzoyl-KLQSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

3700

2-aminobenzoyl-KLRPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

19000

2-aminobenzoyl-KLRSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

2-aminobenzoyl-KLSPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

160

2-aminobenzoyl-KLSSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

2-aminobenzoyl-KLVSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

35000

2-aminobenzoyl-KLYPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

78000

2-aminobenzoyl-KLYSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]

Scytalidium lignicola

in 100 mM sodium acetate buffer (pH 4.5) at 37°C

709118

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SPECIFIC ACTIVITY [µmol/min/mg]

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

additional information

4 entries

additional information

Ganoderma lucidum

30767

additional information

Lentinula edodes

30766

additional information

Scytalidium lignicola

30754

additional information

Scytalidium lignicola

668804

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pH OPTIMUM

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

2 entries

2 - 2.2

Scytalidium lignicola

2.7

casein

2.9

hemoglobin

3.2

hemoglobin

4.5

Scytalidium lignicola

709118

Ganoderma lucidum

casein

30767

Scytalidium lignicola

substrate casein

668804

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pH RANGE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

1.3 - 3.5

Scytalidium lignicola

1.3: about 80% of activity maximum, 3.5: about 35% of activity maximum

30754

2 - 5

Ganoderma lucidum

2: about 30% of activity maximum, 5: about 50% of activity maximum, hemoglobin

30767

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TEMPERATURE OPTIMUM

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

Scytalidium lignicola

30754

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TEMPERATURE RANGE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

37 - 68

Scytalidium lignicola

37°C: about 30% of activity maximum, 68°C: about 50% of activity maximum

30754

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pI VALUE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

3.2

Scytalidium lignicola

668804

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ORGANISM

COMMENTARY

LITERATURE

UNIPROT

SEQUENCE DB

SOURCE

similar enzyme

brenda

similar enzyme

brenda

Scytalidium lignicola

4 entries

Scytalidium lignicola

30559, 30758, 30764, 30765, 670664, 685437, 688338, 709118

brenda

Scytalidium lignicola

753080

P15369

UniProt

brenda

Scytalidium lignicola

ATCC 24568

30754, 30755, 30759, 30762

brenda

Scytalidium lignicola

strain ATCC 24568

668804

brenda

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SOURCE TISSUE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

SOURCE

brenda

brenda

Scytalidium lignicola

30754

brenda

culture filtrate

Scytalidium lignicola A-2

brenda

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LOCALIZATION

ORGANISM

UNIPROT

COMMENTARY

GeneOntology No.

LITERATURE

SOURCE

extracellular

Scytalidium lignicola

the enzyme is secreted

668804

brenda

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UNIPROT

ENTRY NAME

ORGANISM

NO. OF AA

NO. OF TRANSM. HELICES

MOLECULAR WEIGHT[Da]

SOURCE

SEQUENCE

LOCALIZATION PREDICTION

The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).

P15369 pBLAST

PRTB_SCYLI

Scytalidium lignicola

260

27165

Swiss-Prot

Show Sequence

Secretory Pathway (Reliability: 3)

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PDB

SCOP

CATH

UNIPROT

ORGANISM

4 entries

Scytalidium lignicola

1s2b, download, 3D-view

SCOPe (1s2b)

CATH (1s2b)

P15369

Scytalidium lignicola

1s2k, download, 3D-view

SCOPe (1s2k)

CATH (1s2k)

P15369

Scytalidium lignicola

2ifr, download, 3D-view

SCOPe (2ifr)

CATH (2ifr)

P15369

Scytalidium lignicola

2ifw, download, 3D-view

SCOPe (2ifw)

CATH (2ifw)

P15369

Scytalidium lignicola

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MOLECULAR WEIGHT

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

21969

Scytalidium lignicola

1 * 21969, amino acid sequence calculation

668804

21970

Scytalidium lignicola

Scytalidium lignicolum, calculation from amino acid sequence

30764

22000

Scytalidium lignicola

Scytalidium lignicolum, gel filtration

30754

32000

Lentinula edodes

Lentinus edodes, gel filtration

30766

36000

Ganoderma lucidum

Ganoderma lucidum, gel filtration

30767

40000

Lentinula edodes

1 * 40000, Lentinus edodes, SDS-PAGE

30766

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SUBUNIT

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

monomer

3 entries

additional information

Scytalidium lignicola

molecular structure analysis

670664

monomer

Ganoderma lucidum

1 * 36000, Ganoderma lucidum, SDS-PAGE

30767

monomer

Lentinula edodes

1 * 40000, Lentinus edodes, SDS-PAGE

30766

monomer

Scytalidium lignicola

1 * 21969, amino acid sequence calculation

668804

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POSTTRANSLATIONAL MODIFICATION

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

additional information

3 entries

additional information

Lentinula edodes

no glycoprotein

30766

additional information

Scytalidium lignicola

no glycoprotein

30754

additional information

Scytalidium lignicola A-2

no glycoprotein

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CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

enzyme-(acetyl-FKF-(3S,4S)-phenylstatinyl)-LR-NH2 complex and enzyme-acetyl-FKF(2R,3S)-phenylisoseryl-ALR-NH2 complex. Native crystals of the enzyme are grown at room temperature by the hanging-drop vapor diffusion method from 42% saturated ammonium sulfate, 10% ethylene glycol and 0.1 M sodium acetate buffer (pH 4.0). These crystals are then soaked in solutions of varying concentrations of the transition state analogs (acetyl-FKF-(3S,4S)-phenylstatinyl)-LR-NH2 and acetyl-FKF(2R,3S)-phenylisoseryl-ALR-NH2

Scytalidium lignicola

688338

purified enzyme complexed with angiotensin II, hanging drop method, room temperature, from 42% saturated ammonium sulfate, 0.1 M sodium acetate, pH 4.0, 10% v/v ethylene glycol, crystals are soaked in cryosolution containing 30% glvcerol, 45% saturated ammonium sulfate,and 0.1 M sodium acetate, pH 4.0, heavy atom derivatizing, X-ray diffraction structure determination and analysis at 1.9-2.5 A resolution, molecular modeling and multiple isomorphous replacement phasing

Scytalidium lignicola

670664

the three-dimensional structure of SGP is determined by X-ray crystallography. The polypeptide folds into a beta-sandwich that has seven antiparallel beta-strands in each of the two sheets

Scytalidium lignicola

685437

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PROTEIN VARIANTS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

D43A

Scytalidium lignicola

P15369

the mutant has approximately 20% of the original activity

753080

E136A

Scytalidium lignicola

mutant loses the ability for self-activation and cleavage of the propeptide. Mutant has no peptidase activity

685437

Q53A

Scytalidium lignicola

mutant loses the ability for self-activation and cleavage of the propeptide. Mutant has no peptidase activity

685437

Q53E

Scytalidium lignicola

mutant loses the ability for self-activation and cleavage of the propeptide. Mutant has no peptidase activity

685437

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pH STABILITY

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

1.5 - 5

Scytalidium lignicola

37°C, 20 h

30754

3.2 - 5.2

2 entries

6 entries

8 - 9.5

Scytalidium lignicola

in the pH range 8.0-9.5, the enzyme becomes irreversibly inactivated

709118

3.2 - 5.2

Ganoderma lucidum

37°C, 3 h, stable

30767

3.2 - 5.2

Lentinula edodes

37°C, 3 h, stable

30766

Ganoderma lucidum

58°C

30767

Ganoderma lucidum

stable below 42°C, 30 min

30767

Lentinula edodes

stable below 42°C, 30 min

30766

Lentinula edodes

30 min, complete loss of activity at 55°C

30766

Scytalidium lignicola

stable below 60°C, 15 min

30754

Scytalidium lignicola

15 min, complete loss of activity at 68°C

30754

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TEMPERATURE STABILITY

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

3 entries

2 entries

Lentinula edodes

pH 4.0, 30 min, complete loss of activity

30766

Ganoderma lucidum

pH 4, 30 min, complete loss of activity

30767

Scytalidium lignicola

pH 4.0, 15 min, stable below

30754

Scytalidium lignicola

pH 4.0, 15 min, complete loss of activity

30754

Ganoderma lucidum

pH 3.2-5.2, 3 h, stable

30767

Lentinula edodes

pH 3.2-5.2, 3 h, stable

30766

Scytalidium lignicola

pH 1.5-5.5, 20 h, stable

30754

Ganoderma lucidum

pH 4.0, 30 min, stable below

30767

Lentinula edodes

pH 4.0, 30 min, stable below

30766

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

3 entries

Lentinula edodes

30766

Scytalidium lignicola

Go to Cloned (commentary) Search

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CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

DNA and amino acid sequence determination and analysis, expression in Saccharomyces cerevisiae with propper processing prior to secretion

Scytalidium lignicola

668804

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REF.

AUTHORS

TITLE

JOURNAL

VOL.

PAGES

YEAR

ORGANISM (UNIPROT)

PUBMED ID

SOURCE

30559

Majima, E.; Oda, K.; Murao, S.; Ichishima, E.

Comparative study on the specificities of several fungal aspartic and acidic proteinases towards the tetradecapeptide of a renin substrate

Agric. Biol. Chem.

787-793

1988

Scytalidium lignicola

brenda

30754

Oda, K.; Murao, S.

New acid proteases from Scytalidium lignicolum M-133. II. Purification and some enzymatic properties of acid protease A and B of Scytalidium lignicolum ATCC 24568.

Agric. Biol. Chem.

2435-2444

1974

Scytalidium lignicola

brenda

30755

Oda, K.; Murao, S.

Studies on new acid proteases from Scytalidium lignicolum M-133. Part V. Action of Scytalidium lignicolum acid proteases on the insulin B-chain

Agric. Biol. Chem.

1221-1225

1976

Scytalidium lignicola

brenda

30758

Ichishima, E.; Majima, E.; Emi, M.; Hayashi, K.; Murao, S.

Enzymic cleavage of the histidyl-prolyl bond of proangiotensin by carboxyl proteinases from Aspergillus sojae and Scytalidium lignicolum

Agric. Biol. Chem.

2391-2393

1981

Scytalidium lignicola

brenda

30759

Morihara, K.; Tsuzuki, H.; Murao, S.; Oda, K.

Pepstatin-insenstive acid proteases from Scytalidium lignicolum. Kinetic study with synthetic peptides

J. Biochem.

661-668

1979

Scytalidium lignicola

34596

brenda

30762

Oda, K.; Murao, S.

Studies on new acid proteases from Scytalidium lignicolum ATCC 24568. Part IX. Additional evidence for the identity of Scytalidium lignicolum acid proteinases with the carboxyl proteinase group: the interaction between angiotensin I and S-PI-insensitive acid proteinases by means of a zinc(II)-dye complex as a probe

Agric. Biol. Chem.

1995-2001

1986

Scytalidium lignicola

brenda

30764

Maita, T.; Nagata, S.; Matsuda, G.; Maruta, S.; Oda, K.; Murao, S.; Tsuru, D.

Complete amino acid sequence of Scytalidium lignicolum acid protease B

J. Biochem.

465-475

1984

Scytalidium lignicola

6370989

brenda

30765

Tsuru, D.; Shimada, S.; Maruta, S.; Yoshimoto, T.; Oda, K.; Murao, S.; Miyata, T.; Iwanaga, S.

Isolation and amino acid sequence of a peptide containing an epoxide-reactive residue from the thermolysin-digest of Scytalidium lignicolum acid protease B

J. Biochem.

1537-1539

1986

Scytalidium lignicola

3519605

brenda

30766

Terashita, T.; Oda, K.; Kono, M.; Murao, S.

Streptomyces pepsin inhibitor-insensitive carboxyl proteinase from Lentinus edodes

Agric. Biol. Chem.

1937-1943

1981

Lentinula edodes

brenda

30767

Terashita, T.; Oda, K.; Kono, M.; Murao, S.

Streptomyces pepsin inhibitor-insensitive carboxyl proteinase from Ganoderma lucidum

Agric. Biol. Chem.

1029-1035

1984

Ganoderma lucidum

brenda

668804

Oda, K.

Scytalidopepsin B

Handbook of Proteolytic Enzymes (Barrett, J. ; Rawlings, N. D. ; Woessner, J. F. , eds. )

219-221

2004

Scytalidium lignicola

brenda

670664

Fujinaga, M.; Cherney, M.M.; Oyama, H.; Oda, K.; James, M.N.

The molecular structure and catalytic mechanism of a novel carboxyl peptidase from Scytalidium lignicolum

Proc. Natl. Acad. Sci. USA

101

3364-3369

2004

Scytalidium lignicola

14993599

brenda

685437

James, M.N.

The peptidases from fungi and viruses

Biol. Chem.

387

1023-1029

2006

Scytalidium lignicola

16895471

brenda

688338

Pillai, B.; Cherney, M.M.; Hiraga, K.; Takada, K.; Oda, K.; James, M.N.

Crystal structure of Scytalidoglutamic peptidase with its first potent inhibitor provides insights into substrate specificity and catalysis

J. Mol. Biol.

365

343-361

2007

Scytalidium lignicola

17069854

brenda

709118

Kondo, M.Y.; Okamoto, D.N.; Santos, J.A.; Juliano, M.A.; Oda, K.; Pillai, B.; James, M.N.; Juliano, L.; Gouvea, I.E.

Studies on the catalytic mechanism of a glutamic peptidase

J. Biol. Chem.

285

21437-21445

2010

Scytalidium lignicola

20442413

brenda

753080

Stocchi, N.; Revuelta, M.; Castronuovo, P.; Vera, D.; ten Have, A.

Molecular dynamics and structure function analysis show that substrate binding and specificity are major forces in the functional diversification of eqolisins

BMC Bioinformatics

338

2018

Scytalidium lignicola (P15369)

30249179

brenda

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EXTERNAL LINKS (specific for EC-Number 3.4.23.32)

ExplorEnz (official portal for IUBMB Enzyme Nomenclature)

Expasy Enzyme Nomenclature Database

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MetaCyc

SABIO-RK

NCBI: PubMed, Protein, Nucleotide, Structure, Gene, OMIM

Enzyme Nomenclature (alternative site)

UniProt

PDB

PROSITE Database of protein families and domains

InterPro (database of protein families, domains and functional sites)

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