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EC Tree
The expected taxonomic range for this enzyme is: Crotalus atrox
Reaction Schemes
Cleavage of His5-/-Leu, Ser9-/-His, His10-/-Leu, Ala14-/-Leu and Tyr16-/-Leu of insulin B chain
Synonyms
atroxase,
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Atroxase (Crotalus atrox)
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Crotalus atrox fibrinogenase
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Crotalus atrox nonhemorrhagic metalloendopeptidase
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Cleavage of His5-/-Leu, Ser9-/-His, His10-/-Leu, Ala14-/-Leu and Tyr16-/-Leu of insulin B chain
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hydrolysis of peptide bond
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Dimethylcasein + H2O
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Fibrin + H2O
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both alpha and beta chains hydrolyzed, but the gamma-gamma chain not affected
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Fibrinogen + H2O
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Aalpha and Bbeta chains readily hydrolyzed, but gamma chain resistant to hydrolysis
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Oxidized insulin B-chain
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cleavage of His5-Leu6, Ser9-His10, His10-Leu11, Ala14-Leu15, Tyr16-Leu17
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Oxidized insulin B-chain + H2O
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additional information
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Human fibrinogen + H2O
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gamma chain is not hydrolyzed
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Human fibrinogen + H2O
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hydrolysis of Aalpha and Bbeta chain, cleavage pattern different from thrombin cleavage pattern
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additional information
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no hemorrhagic activity, no substrates are thrombin, synthetic substrate for thrombin S-2238
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additional information
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nontoxic venom component, nonhemorrhagic endopeptidase
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additional information
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no hydrolysis of the synthetic substrate S-2238 for thrombin
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additional information
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nontoxic venom component, nonhemorrhagic endopeptidase
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Ca2+
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0.3 mol/mol enzyme
Zn2+
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class I zinc metalloproteinase, contains 1 mol zinc/mol enzyme, no activation by added Zn2+
Zn2+
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zinc-binding sequence
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alpha2-Macroglobulin
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additional information
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no inhibition by aprotinin, PMSF or soybean trypsin inhibitor
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additional information
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1.67 mg fibrinogen/min mg enzyme
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additional information
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pI: 9.6
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brenda
Western diamond rattlesnake
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brenda
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brenda
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VM1AT_CROAT
203
0
23204
Swiss-Prot
other Location (Reliability: 2)
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23500
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sequence analysis
23500
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x * 23500, Crotalus atrox, SDS-PAGE
additional information
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amino acid composition
additional information
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amino acid sequence
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x * 23500, Crotalus atrox, SDS-PAGE
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by cellulose column chromatography
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from lyophilized crude venom
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Crotalus atrox, expressed in Escherichia coli
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medicine
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no necrosis or hemorrhage, thrombolysis
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Willis, T.W.; Tu, A.T.
Purification and biochemical characterization of atroxase, a nonhemorrhagic fibrinolytic protease from western diamondback rattlesnake venom
Biochemistry
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4769-4777
1988
Crotalus atrox
brenda
Baker, B.J.; Wongvibulsin, S.; Nyborg, J.; Tu, A.T.
Nucleotide sequence encoding the snake venom fibrinolytic enzyme atroxase obtained from a Crotalus atrox venom gland cDNA library
Arch. Biochem. Biophys.
317
357-364
1995
Crotalus atrox
brenda
Tu, A.T.
Atroxase
Handbook Of Proteolytic Enzymes(Barrett,A. J. ,Rawlings,N. D. ,Woessner,J. F. ,Eds. )Academic Press
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677-678
2004
Crotalus atrox
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brenda
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