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(2R,3R,4R)-6-[(aminooxy)carbonyl]-3,4,6-trihydroxy-N-propyltetrahydro-2H-pyran-2-carboxamide
?
-
-
-
-
?
(2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid
?
-
-
-
-
?
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
pyruvate + (2R,3S)-2,3-dihydroxy-4-oxo-N,N-dipropylbutanamide
-
-
-
-
r
2-deoxy-2-[[(alpha-D-galactopyranosyloxy)acetyl]amino]-D-mannopyranose + pyruvate
3,5-dideoxy-5-[[(alpha-D-galactopyranosyloxy)acetyl]amino]-D-glycero-beta-D-galacto-non-2-ulopyranosonic acid
-
-
36% yield
-
?
2-deoxy-2-[[(beta-D-galactopyranosyloxy)acetyl]amino]-D-mannopyranose + pyruvate
3,5-dideoxy-5-[[(beta-D-galactopyranosyloxy)acetyl]amino]-D-glycero-beta-D-galacto-non-2-ulopyranosonic acid
-
-
34% yield
-
?
2-deoxy-D-glucose + pyruvate
?
3-bromo-3-deoxy-D-mannose + pyruvate
?
-
wild-type enzyme: 5% of the activity with N-acetyl-D-mannosamine, mutant enzyme Y98H/F115L/V251I: 5% of the activity with N-acetyl-D-mannosamine, mutant enzyme Y98H/F115L/V251I/V265I: 10% of the activity with N-acetyl-D-mannosamine, mutant enzyme Y98H/F115L/N153Y/V251I/V265I/Y281C: 10% of the activity with N-acetyl-D-mannosamine, mutant enzyme E60A/Y98H/F115L/N153Y/D150G/V251I/V265I/Y281C/N153Y: 10% of the activity with N-acetyl-D-mannosamine
-
-
?
3-deoxy-manno-oct-ulosonic acid
arabinose + pyruvate
-
-
-
?
4-O-alpha-D-galactopyranosyl-D-mannopyranose + pyruvate
3-deoxy-7-O-alpha-D-galactopyranosyl-D-glycero-beta-D-galacto-non-2-ulopyranosonic acid
-
-
5% yield
-
?
4-O-beta-D-galactopyranosyl-D-mannopyranose + pyruvate
3-deoxy-7-O-beta-D-galactopyranosyl-D-glycero-beta-D-galacto-non-2-ulopyranosonic acid
-
-
38% yield
-
?
4-O-beta-D-glucopyranosyl-D-mannopyranose + pyruvate
3-deoxy-7-O-beta-D-glucopyranosyl-D-glycero-beta-D-galacto-non-2-ulopyranosonic acid
-
-
35% yield
-
?
5-N-acetyl-4-O-acetylneuraminic acid
?
-
22% the activity with N-acetylneuraminic acid
-
?
5-N-acetyl-9-O-acetylneuraminic acid
?
5-O-alpha-D-galactopyranosyl-alpha-D-mannofuranose + pyruvate
3-deoxy-8-O-alpha-D-galactopyranosyl-D-glycero-beta-D-galacto-non-2-ulopyranosonic acid
-
-
62% yield
-
?
5-O-beta-D-galactopyranosyl-alpha-D-mannofuranose + pyruvate
3-deoxy-8-O-beta-D-galactopyranosyl-D-glycero-beta-D-galacto-non-2-ulopyranosonic acid
-
-
85% yield
-
?
5-O-methyl-N-acetyl-D-mannosamine + pyruvate
8-O-methyl-N-acetylneuraminate
-
-
-
?
6-O-alpha-D-galactopyranosyl-D-mannopyranose + pyruvate
3-deoxy-9-O-alpha-D-galactopyranosyl-D-glycero-beta-D-galacto-non-2-ulopyranosonic acid
-
-
81% yield
-
?
6-O-alpha-D-glucopyranosyl-D-mannopyranose + pyruvate
3-deoxy-9-O-alpha-D-glucopyranosyl-D-glycero-beta-D-galacto-non-2-ulopyranosonic acid
-
-
65% yield
-
?
6-O-alpha-D-mannopyranosyl-D-mannopyranose + pyruvate
3-deoxy-9-O-alpha-D-mannopyranosyl-D-glycero-beta-D-galacto-non-2-ulopyranosonic acid
-
-
78% yield
-
?
6-O-beta-D-glucopyranosyl-D-mannopyranose + pyruvate
3-deoxy-9-O-beta-D-glucopyranosyl-D-glycero-beta-D-galacto-non-2-ulopyranosonic acid
-
-
83% yield
-
?
D-3-deoxy-manno-2-octulosonic acid
D-arabinose + pyruvate
-
-
-
-
r
D-arabinose + pyruvate
3-deoxy-D-manno-2-octulosonic acid
D-arabinose + pyruvate
D-3-deoxy-manno-2-octulosonic acid
-
wild-type enzyme: 20% of the activity with N-acetyl-D-mannosamine, mutant enzyme Y98H/F115L/V251I: 40% of the activity with N-acetyl-D-mannosamine, mutant enzyme Y98H/F115L/V251I/V265I: 65% of the activity with N-acetyl-D-mannosamine, mutant enzyme Y98H/F115L/N153Y/V251I/V265I/Y281C: 40% of the activity with N-acetyl-D-mannosamine, mutant enzyme E60A/Y98H/F115L/N153Y/D150G/V251I/V265I/Y281C/N153Y: 85% of the activity with N-acetyl-D-mannosamine
-
-
r
D-glucose + pyruvate
?
-
wild-type enzyme: 40% of the activity with N-acetyl-D-mannosamine, mutant enzyme Y98H/F115L/V251I: 40% of the activity with N-acetyl-D-mannosamine, mutant enzyme Y98H/F115L/V251I/V265I: 20% of the activity with N-acetyl-D-mannosamine, mutant enzyme Y98H/F115L/N153Y/V251I/V265I/Y281C: 25% of the activity with N-acetyl-D-mannosamine, mutant enzyme E60A/Y98H/F115L/N153Y/D150G/V251I/V265I/Y281C/N153Y: 20% of the activity with N-acetyl-D-mannosamine
-
-
?
D-gulose + pyruvate
?
-
wild-type enzyme: 10% of the activity with N-acetyl-D-mannosamine, mutant enzyme Y98H/F115L/V251I/V265I: 110% of the activity with N-acetyl-D-mannosamine, mutant enzyme Y98H/F115L/N153Y/V251I/V265I/Y281C: 90% of the activity with N-acetyl-D-mannosamine, mutant enzyme E60A/Y98H/F115L/N153Y/D150G/V251I/V265I/Y281C/N153Y: 70% of the activity with N-acetyl-D-mannosamine
-
-
?
D-mannosamine + pyruvate
?
-
wild-type enzyme: 80% of the activity with N-acetyl-D-mannosamine, mutant enzyme Y98H/F115L/V251I: 90% of the activity with N-acetyl-D-mannosamine, mutant enzyme Y98H/F115L/V251I/V265I: 165% of the activity with N-acetyl-D-mannosamine, mutant enzyme Y98H/F115L/N153Y/V251I/V265I/Y281C: 190% of the activity with N-acetyl-D-mannosamine, mutant enzyme E60A/Y98H/F115L/N153Y/D150G/V251I/V265I/Y281C/N153Y: 170% of the activity with N-acetyl-D-mannosamine
-
-
?
D-mannose + pyruvic acid
2-keto-3-deoxy-D-glycero-D-galacto-nonopyranulosonic acid
-
-
-
-
?
D-talose + pyruvate
?
-
wild-type enzyme: 60% of the activity with N-acetyl-D-mannosamine, mutant enzyme Y98H/F115L/V251I: 50% of the activity with N-acetyl-D-mannosamine, mutant enzyme Y98H/F115L/V251I/V265I: 105% of the activity with N-acetyl-D-mannosamine, mutant enzyme Y98H/F115L/N153Y/V251I/V265I/Y281C: 80% of the activity with N-acetyl-D-mannosamine, mutant enzyme E60A/Y98H/F115L/N153Y/D150G/V251I/V265I/Y281C/N153Y: 70% of the activity with N-acetyl-D-mannosamine
-
-
?
L-3-deoxy-manno-2-octulosonic acid
L-arabinose + pyruvate
-
-
-
-
r
L-arabinose + ?
3-deoxy-L-manno-oct-2-ulosonic acid
-
-
?
L-arabinose + pyruvate
L-3-deoxy-manno-2-octulosonic acid
-
wild-type enzyme: 10% of the activity with N-acetyl-D-mannosamine, mutant enzyme Y98H/F115L/V251I: 35% of the activity with N-acetyl-D-mannosamine, mutant enzyme Y98H/F115L/V251I/V265I: 70% of the activity with N-acetyl-D-mannosamine, mutant enzyme Y98H/F115L/N153Y/V251I/V265I/Y281C: 40% of the activity with N-acetyl-D-mannosamine, mutant enzyme E60A/Y98H/F115L/N153Y/D150G/V251I/V265I/Y281C/N153Y: 80% of the activity with N-acetyl-D-mannosamine
-
-
r
L-mannose + pyruvate
?
-
wild-type enzyme: 80% of the activity with N-acetyl-D-mannosamine, mutant enzyme Y98H/F115L/V251I: 110% of the activity with N-acetyl-D-mannosamine, mutant enzyme Y98H/F115L/V251I/V265I: 95% of the activity with N-acetyl-D-mannosamine, mutant enzyme Y98H/F115L/N153Y/V251I/V265I/Y281C: 95% of the activity with N-acetyl-D-mannosamine, mutant enzyme E60A/Y98H/F115L/N153Y/D150G/V251I/V265I/Y281C/N153Y: 95% of the activity with N-acetyl-D-mannosamine
-
-
?
Methoxyneuraminic acid
?
-
-
-
-
?
N,O-Diacetylneuramininic acid
?
-
-
-
-
?
N-Acetyl-4-O-acetylneuraminic acid
?
-
-
-
-
?
N-Acetyl-7-O-acetylneuraminic acid
?
-
-
-
-
?
N-Acetyl-8-O-acetylneuraminic acid
?
-
-
-
-
?
N-Acetyl-9-O-acetylneuraminic acid
?
-
-
-
-
?
N-acetyl-D-mannosamine + pyruvate
N-acetyl-D-neuraminic acid
-
-
-
-
r
N-acetyl-D-mannosamine + pyruvate
N-acetylneuraminate
N-Acetyl-D-mannosamine + pyruvate
N-Acetylneuraminic acid
N-acetyl-D-mannosamine + pyruvic acid
N-acetyl-D-neuraminic acid
-
-
-
-
?
N-acetyl-D-neuraminic acid
N-acetyl-D-mannosamine + pyruvate
N-acetyl-D-neuraminic acid
pyruvate + N-acetyl-D-mannosamine
N-acetyl-L-mannosamine + pyruvate
N-acetyl-L-neuraminic acid
-
wild-type enzyme: 0.2% of the activity with N-acetyl-D-mannosamine, mutant enzyme Y98H/F115L/V251I: 0.5% of the activity with N-acetyl-D-mannosamine, mutant enzyme Y98H/F115L/V251I/V265I: 1% of the activity with N-acetyl-D-mannosamine, mutant enzyme Y98H/F115L/N153Y/V251I/V265I/Y281C: 2% of the activity with N-acetyl-D-mannosamine, mutant enzyme E60A/Y98H/F115L/N153Y/D150G/V251I/V265I/Y281C/N153Y: 2% of the activity with N-acetyl-D-mannosamine
-
-
r
N-acetyl-L-neuraminic acid
N-acetyl-L-mannosamine + pyruvate
-
-
-
-
r
N-acetyl-neuraminic acid
N-acetyl-D-mannosamine + pyruvate
N-Acetylmannosamine + pyruvate
?
N-acetylmannosamine + pyruvate
N-acetylneuraminic acid
-
-
-
-
r
N-acetylneuraminate
N-acetyl-D-mannosamine + pyruvate
N-Acetylneuraminic acid
?
N-Acetylneuraminic acid
N-Acetyl-D-mannosamine + pyruvate
N-acetylneuraminic acid
N-acetylmannosamine + pyruvate
-
-
-
-
r
N-acetylneuraminic acid
pyruvate + N-acetyl-D-mannosamine
-
-
-
-
r
N-Formylneuraminic acid
?
N-Glycolylneuraminic acid
?
N-Monochloracetylneuraminic acid
?
N-Monofluoracetylneuraminic acid
?
-
-
-
-
?
N-Succinylneuraminic acid
?
-
-
-
-
?
pyruvate + alpha-D-Galp-(1,6)-D-Manp
alpha-D-Galp-(1,9)-D-deoxyneuraminic acid
-
-
-
-
?
additional information
?
-
2-deoxy-D-glucose + pyruvate
?
-
33% activity compared to N-acetylneuraminate
-
-
r
2-deoxy-D-glucose + pyruvate
?
-
33% activity compared to N-acetylneuraminate
-
-
r
2-deoxy-D-glucose + pyruvate
?
1.2% activity compared to N-acetylneuraminate
-
-
r
2-deoxy-D-glucose + pyruvate
?
1.2% activity compared to N-acetylneuraminate
-
-
r
5-N-acetyl-9-O-acetylneuraminic acid
?
-
54% the activity with N-acetylneuraminic acid
-
?
5-N-acetyl-9-O-acetylneuraminic acid
?
-
32.45% the activity with N-acetylneuraminic acid
-
?
5-N-acetyl-9-O-acetylneuraminic acid
?
-
45% the activity with N-acetylneuraminic acid
-
?
D-arabinose + pyruvate
3-deoxy-D-manno-2-octulosonic acid
-
1.0% activity compared to N-acetylneuraminate
-
-
r
D-arabinose + pyruvate
3-deoxy-D-manno-2-octulosonic acid
1.5% activity compared to N-acetylneuraminate
-
-
r
D-mannose + pyruvate
?
-
wild-type enzyme: 30% of the activity with N-acetyl-D-mannosamine, mutant enzyme Y98H/F115L/V251I: 15% of the activity with N-acetyl-D-mannosamine, mutant enzyme Y98H/F115L/V251I/V265I: 70% of the activity with N-acetyl-D-mannosamine, mutant enzyme Y98H/F115L/N153Y/V251I/V265I/Y281C: 80% of the activity with N-acetyl-D-mannosamine, mutant enzyme E60A/Y98H/F115L/N153Y/D150G/V251I/V265I/Y281C/N153Y: 75% of the activity with N-acetyl-D-mannosamine
-
-
?
D-mannose + pyruvate
?
-
95% activity compared to N-acetylneuraminate
-
-
r
D-mannose + pyruvate
?
-
95% activity compared to N-acetylneuraminate
-
-
r
D-mannose + pyruvate
?
10% activity compared to N-acetylneuraminate
-
-
r
D-mannose + pyruvate
?
10% activity compared to N-acetylneuraminate
-
-
r
D-xylose + pyruvate
?
-
wild-type enzyme: 45% of the activity with N-acetyl-D-mannosamine, mutant enzyme Y98H/F115L/V251I: 40% of the activity with N-acetyl-D-mannosamine, mutant enzyme Y98H/F115L/V251I/V265I: 55% of the activity with N-acetyl-D-mannosamine, mutant enzyme Y98H/F115L/N153Y/V251I/V265I/Y281C: 25% of the activity with N-acetyl-D-mannosamine, mutant enzyme E60A/Y98H/F115L/N153Y/D150G/V251I/V265I/Y281C/N153Y: 25% of the activity with N-acetyl-D-mannosamine
-
-
?
D-xylose + pyruvate
?
-
2.4% activity compared to N-acetylneuraminate
-
-
r
D-xylose + pyruvate
?
-
2.4% activity compared to N-acetylneuraminate
-
-
r
D-xylose + pyruvate
?
0.6% activity compared to N-acetylneuraminate
-
-
r
D-xylose + pyruvate
?
0.6% activity compared to N-acetylneuraminate
-
-
r
L-gulose + pyruvate
?
-
wild-type enzyme: 70% of the activity with N-acetyl-D-mannosamine, mutant enzyme Y98H/F115L/V251I: 35% of the activity with N-acetyl-D-mannosamine, mutant enzyme Y98H/F115L/V251I/V265I: 15% of the activity with N-acetyl-D-mannosamine, mutant enzyme Y98H/F115L/N153Y/V251I/V265I/Y281C: 15% of the activity with N-acetyl-D-mannosamine, mutant enzyme E60A/Y98H/F115L/N153Y/D150G/V251I/V265I/Y281C/N153Y: 15% of the activity with N-acetyl-D-mannosamine
-
-
?
L-gulose + pyruvate
?
-
3.6% activity compared to N-acetylneuraminate
-
-
r
L-gulose + pyruvate
?
-
3.6% activity compared to N-acetylneuraminate
-
-
r
L-gulose + pyruvate
?
0.7% activity compared to N-acetylneuraminate
-
-
r
L-gulose + pyruvate
?
0.7% activity compared to N-acetylneuraminate
-
-
r
N-acetyl-D-mannosamine + pyruvate
N-acetylneuraminate
-
-
-
r
N-acetyl-D-mannosamine + pyruvate
N-acetylneuraminate
-
-
-
r
N-acetyl-D-mannosamine + pyruvate
N-acetylneuraminate
-
-
-
r
N-acetyl-D-mannosamine + pyruvate
N-acetylneuraminate
the enzyme catalyzes the retro-aldol cleavage of N-acetylneuraminic acid to form N-acetyl-D-mannosamine and pyruvate
-
-
r
N-acetyl-D-mannosamine + pyruvate
N-acetylneuraminate
the enzyme catalyzes the retro-aldol cleavage of N-acetylneuraminic acid to form N-acetyl-D-mannosamine and pyruvate
-
-
r
N-acetyl-D-mannosamine + pyruvate
N-acetylneuraminate
reversible aldol condensation of pyruvate and N-acetyl-D-mannosamine
-
-
r
N-acetyl-D-mannosamine + pyruvate
N-acetylneuraminate
reversible aldol condensation of pyruvate and N-acetyl-D-mannosamine
-
-
r
N-Acetyl-D-mannosamine + pyruvate
N-Acetylneuraminic acid
-
-
-
r
N-Acetyl-D-mannosamine + pyruvate
N-Acetylneuraminic acid
-
-
-
r
N-Acetyl-D-mannosamine + pyruvate
N-Acetylneuraminic acid
-
-
-
r
N-Acetyl-D-mannosamine + pyruvate
N-Acetylneuraminic acid
-
-
-
-
r
N-Acetyl-D-mannosamine + pyruvate
N-Acetylneuraminic acid
-
-
-
r
N-Acetyl-D-mannosamine + pyruvate
N-Acetylneuraminic acid
-
-
-
r
N-Acetyl-D-mannosamine + pyruvate
N-Acetylneuraminic acid
-
-
-
r
N-Acetyl-D-mannosamine + pyruvate
N-Acetylneuraminic acid
-
-
-
r
N-Acetyl-D-mannosamine + pyruvate
N-Acetylneuraminic acid
-
-
-
r
N-acetyl-D-neuraminic acid
N-acetyl-D-mannosamine + pyruvate
-
in presence of excess pyruvate the equilibrium may be driven in the direction of aldol product formation
-
-
r
N-acetyl-D-neuraminic acid
N-acetyl-D-mannosamine + pyruvate
-
-
-
-
?
N-acetyl-D-neuraminic acid
N-acetyl-D-mannosamine + pyruvate
-
-
-
-
r
N-acetyl-D-neuraminic acid
N-acetyl-D-mannosamine + pyruvate
-
-
-
r
N-acetyl-D-neuraminic acid
pyruvate + N-acetyl-D-mannosamine
-
-
-
-
r
N-acetyl-D-neuraminic acid
pyruvate + N-acetyl-D-mannosamine
-
-
-
-
r
N-acetyl-neuraminic acid
N-acetyl-D-mannosamine + pyruvate
-
-
-
-
r
N-acetyl-neuraminic acid
N-acetyl-D-mannosamine + pyruvate
-
-
-
-
r
N-Acetylmannosamine + pyruvate
?
-
-
-
-
?
N-Acetylmannosamine + pyruvate
?
-
N-acetylneuraminic acid is intermediate for colomic acid biosynthesis
-
-
?
N-acetylneuraminate
N-acetyl-D-mannosamine + pyruvate
-
-
-
-
r
N-acetylneuraminate
N-acetyl-D-mannosamine + pyruvate
-
-
-
r
N-acetylneuraminate
N-acetyl-D-mannosamine + pyruvate
-
-
-
-
r
N-acetylneuraminate
N-acetyl-D-mannosamine + pyruvate
-
-
-
r
N-acetylneuraminate
N-acetyl-D-mannosamine + pyruvate
-
-
-
-
r
N-acetylneuraminate
N-acetyl-D-mannosamine + pyruvate
-
-
-
r
N-acetylneuraminate
N-acetyl-D-mannosamine + pyruvate
-
-
-
-
r
N-acetylneuraminate
N-acetyl-D-mannosamine + pyruvate
-
-
-
r
N-acetylneuraminate
N-acetyl-D-mannosamine + pyruvate
-
-
-
-
r
N-acetylneuraminate
N-acetyl-D-mannosamine + pyruvate
-
-
-
r
N-acetylneuraminate
N-acetyl-D-mannosamine + pyruvate
-
-
-
-
r
N-acetylneuraminate
N-acetyl-D-mannosamine + pyruvate
-
the enzyme catalyzes the reversible condensation of pyruvate with N-acetyl-D-mannosamine to yield the sialic acid N-acetylneuraminic acid
-
-
r
N-acetylneuraminate
N-acetyl-D-mannosamine + pyruvate
-
-
-
?
N-acetylneuraminate
N-acetyl-D-mannosamine + pyruvate
-
100% activity
-
-
r
N-acetylneuraminate
N-acetyl-D-mannosamine + pyruvate
-
100% activity
-
-
r
N-acetylneuraminate
N-acetyl-D-mannosamine + pyruvate
100% activity
-
-
r
N-acetylneuraminate
N-acetyl-D-mannosamine + pyruvate
100% activity
-
-
r
N-acetylneuraminate
N-acetyl-D-mannosamine + pyruvate
-
-
-
r
N-acetylneuraminate
N-acetyl-D-mannosamine + pyruvate
-
-
-
r
N-acetylneuraminate
N-acetyl-D-mannosamine + pyruvate
reversible aldol cleavage of N-acetylneuraminic acid
-
-
r
N-acetylneuraminate
N-acetyl-D-mannosamine + pyruvate
-
-
-
?
N-acetylneuraminate
N-acetyl-D-mannosamine + pyruvate
-
-
-
r
N-acetylneuraminate
N-acetyl-D-mannosamine + pyruvate
-
-
-
r
N-acetylneuraminate
N-acetyl-D-mannosamine + pyruvate
-
-
-
r
N-acetylneuraminate
N-acetyl-D-mannosamine + pyruvate
-
-
-
?
N-acetylneuraminate
N-acetyl-D-mannosamine + pyruvate
-
-
-
r
N-acetylneuraminate
N-acetyl-D-mannosamine + pyruvate
-
-
-
r
N-acetylneuraminate
N-acetyl-D-mannosamine + pyruvate
-
-
-
r
N-acetylneuraminate
N-acetyl-D-mannosamine + pyruvate
-
-
-
-
r
N-Acetylneuraminic acid
?
Corynebacterium belfanti
-
metabolized by pathogens parasitizing on mucous membranes
-
-
?
N-Acetylneuraminic acid
?
-
metabolized by pathogens parasitizing on mucous membranes
-
-
?
N-Acetylneuraminic acid
?
-
metabolized by pathogens parasitizing on mucous membranes
-
-
?
N-Acetylneuraminic acid
?
-
metabolized by pathogens parasitizing on mucous membranes
-
-
?
N-Acetylneuraminic acid
?
-
N-acetylneuraminate lyase plays important role in regulation of N-acetylneuraminic acid metabolism
-
-
?
N-Acetylneuraminic acid
?
-
metabolized by pathogens parasitizing on mucous membranes
-
-
?
N-Acetylneuraminic acid
N-Acetyl-D-mannosamine + pyruvate
-
-
-
r
N-Acetylneuraminic acid
N-Acetyl-D-mannosamine + pyruvate
-
-
-
-
?
N-Acetylneuraminic acid
N-Acetyl-D-mannosamine + pyruvate
-
-
-
-
r
N-Acetylneuraminic acid
N-Acetyl-D-mannosamine + pyruvate
-
-
-
r
N-Acetylneuraminic acid
N-Acetyl-D-mannosamine + pyruvate
-
-
-
r
N-Acetylneuraminic acid
N-Acetyl-D-mannosamine + pyruvate
-
-
-
r
N-Acetylneuraminic acid
N-Acetyl-D-mannosamine + pyruvate
Corynebacterium belfanti
-
-
-
-
?
N-Acetylneuraminic acid
N-Acetyl-D-mannosamine + pyruvate
-
-
-
-
?
N-Acetylneuraminic acid
N-Acetyl-D-mannosamine + pyruvate
-
-
-
-
?
N-Acetylneuraminic acid
N-Acetyl-D-mannosamine + pyruvate
-
-
-
-
?
N-Acetylneuraminic acid
N-Acetyl-D-mannosamine + pyruvate
-
-
-
r
N-Acetylneuraminic acid
N-Acetyl-D-mannosamine + pyruvate
-
-
-
r
N-Acetylneuraminic acid
N-Acetyl-D-mannosamine + pyruvate
-
-
-
r
N-Acetylneuraminic acid
N-Acetyl-D-mannosamine + pyruvate
-
-
-
r
N-Acetylneuraminic acid
N-Acetyl-D-mannosamine + pyruvate
-
-
?
N-Acetylneuraminic acid
N-Acetyl-D-mannosamine + pyruvate
-
-
r
N-Acetylneuraminic acid
N-Acetyl-D-mannosamine + pyruvate
-
syn: Neu5Ac
-
r
N-Acetylneuraminic acid
N-Acetyl-D-mannosamine + pyruvate
-
-
-
-
?
N-Acetylneuraminic acid
N-Acetyl-D-mannosamine + pyruvate
-
-
-
r
N-Acetylneuraminic acid
N-Acetyl-D-mannosamine + pyruvate
-
formation of a Schiff base
-
r
N-Acetylneuraminic acid
N-Acetyl-D-mannosamine + pyruvate
-
-
-
r
N-Acetylneuraminic acid
N-Acetyl-D-mannosamine + pyruvate
-
regulation of sialic acid metabolism
-
r
N-Acetylneuraminic acid
N-Acetyl-D-mannosamine + pyruvate
-
-
-
-
?
N-Acetylneuraminic acid
N-Acetyl-D-mannosamine + pyruvate
-
-
-
r
N-Acetylneuraminic acid
N-Acetyl-D-mannosamine + pyruvate
-
-
-
-
?
N-Acetylneuraminic acid
N-Acetyl-D-mannosamine + pyruvate
-
-
-
r
N-Acetylneuraminic acid
N-Acetyl-D-mannosamine + pyruvate
-
-
-
r
N-Formylneuraminic acid
?
-
-
-
-
?
N-Formylneuraminic acid
?
-
-
-
?
N-Glycolylneuraminic acid
?
-
-
-
-
?
N-Glycolylneuraminic acid
?
-
-
-
?
N-Glycolylneuraminic acid
?
-
-
-
-
?
N-Glycolylneuraminic acid
?
-
60% the rate of N-acetylneuraminic acid
-
?
N-Glycolylneuraminic acid
?
-
-
-
-
?
N-Glycolylneuraminic acid
?
-
-
-
?
N-Glycolylneuraminic acid
?
-
47% the activity with N-acetylneuraminic acid
-
?
N-Monochloracetylneuraminic acid
?
-
-
-
-
?
N-Monochloracetylneuraminic acid
?
-
-
-
?
additional information
?
-
-
structural characteristics of substrates
-
-
?
additional information
?
-
-
configuration of substrate and products
-
-
?
additional information
?
-
-
condensation: N-acetylglucosamine, N-acetylgalactosamine, glucosamine and galactosamine cannot replace N-acetylmannosamine. Phosphoenolpyruvate, lactate, beta-hydroxypyruvate and other pyruvate derivatives cannot replace pyruvate
-
-
?
additional information
?
-
-
no substrates are 3-azido-3-deoxy, 3-amino-3-deoxy and 3-N-tert-butylcarbonyl-3-deoxy derivatives of 2-acetamido-2-deoxy-alpha,beta-D-mannose
-
?
additional information
?
-
One point mutation (L142R) is sufficient to create an enzyme that can complement a bacterial auxotroph lacking the gene for dihydrodipicolinate synthase, EC 4.2.1.52. The mutant enzyme catalyzes the reaction: L-aspartate 4-semialdehyde + pyruvate = dihydrodipicolinate + 2 H2O
-
?
additional information
?
-
-
One point mutation (L142R) is sufficient to create an enzyme that can complement a bacterial auxotroph lacking the gene for dihydrodipicolinate synthase, EC 4.2.1.52. The mutant enzyme catalyzes the reaction: L-aspartate 4-semialdehyde + pyruvate = dihydrodipicolinate + 2 H2O
-
?
additional information
?
-
-
not: N-acetyl-4-O-acetylneuraminic acid and 2-deoxy-2,3-didehydro-N-acetylneuraminic acid
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
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Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
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0.39 - 11
(2R,3R,4R)-6-[(aminooxy)carbonyl]-3,4,6-trihydroxy-N-propyltetrahydro-2H-pyran-2-carboxamide
0.35 - 10.6
(2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid
0.2 - 11
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
3.5 - 14.5
D-3-deoxy-manno-2-octulosonic acid
2.4 - 14.1
L-3-deoxy-manno-2-octulosonic acid
12.5
N-acetyl-4-O-acetylneuraminic acid
-
-
4.5
N-Acetyl-7-O-acetylneuraminic acid
-
-
2
N-Acetyl-8-O-acetylneuraminic acid
-
-
6.9 - 3200
N-acetyl-D-mannosamine
2.1 - 38
N-acetyl-D-neuraminic acid
3 - 369.4
N-acetyl-L-neuraminic acid
2.8
N-acetyl-neuraminic acid
-
in 0.1 M phosphate buffer, pH 7
0.013 - 87.7
N-acetylneuraminate
0.4 - 1100
N-acetylneuraminic acid
1.75
N-Formylneuraminic acid
-
-
2.2 - 4.6
N-glycolylneuraminic acid
2.7
N-Monochloracetylneuraminic acid
-
-
10
N-Monofluoracetylneuraminic acid
-
-
1.93
N-Succinylneuraminic acid
-
-
additional information
additional information
-
with substrate N-acetylneuraminic acid increasing Km with increasing pH
-
0.39
(2R,3R,4R)-6-[(aminooxy)carbonyl]-3,4,6-trihydroxy-N-propyltetrahydro-2H-pyran-2-carboxamide
-
mutant enzyme E192N
11
(2R,3R,4R)-6-[(aminooxy)carbonyl]-3,4,6-trihydroxy-N-propyltetrahydro-2H-pyran-2-carboxamide
-
wild-type enzyme
0.35
(2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid
-
pH 7.5, 25°C, mutant enzyme E192V
0.39
(2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid
-
pH 7.5, 25°C, mutant enzyme E192N
0.5
(2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid
-
pH 7.5, 25°C, mutant enzyme E192S
2.54
(2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid
-
pH 7.5, 25°C, mutant enzyme S208G
5.7
(2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid
-
pH 7.5, 25°C, mutant enzyme D191P
10.6
(2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid
-
pH 7.5, 25°C, wild-type enzyme
0.2
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
-
E192M mutant protein, pH 7.5, 25°C
0.2
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
-
E192P mutant protein, pH 7.5, 25°C
0.3
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
-
E192F mutant protein, pH 7.5, 25°C
0.4
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
-
E192N mutant protein, pH 7.7.5, 25°C
0.4
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
-
E192V mutant protein, pH 7.5, 25°C
0.5
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
-
E192H mutant protein, pH 7.5, 25°C
0.6
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
-
E192S mutant protein, pH 7.5, 25°C
0.7
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
-
E192Q mutant protein, pH 7.5, 25°C
1.1
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
-
E192D mutant protein, pH 7.5, 25°C
11
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
-
wild type (E192), pH 7.5, 25°C
3.5
D-3-deoxy-manno-2-octulosonic acid
-
pH 7.5, 25°C, mutant enzyme Y98H/F115L/V251I/V265I
6.3
D-3-deoxy-manno-2-octulosonic acid
-
pH 7.5, 25°C, mutant enzyme Y98H/F115L/N153Y/V251I/V265I/Y281C
11.4
D-3-deoxy-manno-2-octulosonic acid
-
pH 7.5, 25°C, mutant enzyme E60A/Y98H/F115L/N153Y/D150G/V251I/V265I/Y281C/N153Y
12.3
D-3-deoxy-manno-2-octulosonic acid
-
pH 7.5, 25°C, mutant enzyme Y98H/F115L
13.8
D-3-deoxy-manno-2-octulosonic acid
-
pH 7.5, 25°C, wild-type enzyme
14.5
D-3-deoxy-manno-2-octulosonic acid
-
pH 7.5, 25°C, mutant enzyme Y98H/F115L/V251I
2.4
L-3-deoxy-manno-2-octulosonic acid
-
pH 7.5, 25°C, mutant enzyme Y98H/F115L/N153Y/V251I/V265I/Y281C
2.7
L-3-deoxy-manno-2-octulosonic acid
-
pH 7.5, 25°C, mutant enzyme Y98H/F115L/V251I/V265I
2.8
L-3-deoxy-manno-2-octulosonic acid
-
pH 7.5, 25°C, mutant enzyme E60A/Y98H/F115L/N153Y/D150G/V251I/V265I/Y281C/N153Y
7.5
L-3-deoxy-manno-2-octulosonic acid
-
pH 7.5, 25°C, wild-type enzyme
9.2
L-3-deoxy-manno-2-octulosonic acid
-
pH 7.5, 25°C, mutant enzyme Y98H/F115L/V251I
14.1
L-3-deoxy-manno-2-octulosonic acid
-
pH 7.5, 25°C, mutant enzyme Y98H/F115L
6.9
N-acetyl-D-mannosamine
at pH 7.0 and 37°C
7.7
N-acetyl-D-mannosamine
-
-
53.3
N-acetyl-D-mannosamine
at pH 7.5 and 37°C
92.1
N-acetyl-D-mannosamine
at pH 8.5 and 37°C
131.7
N-acetyl-D-mannosamine
-
recombinant enzyme, pH and temperature not specified in the publication
149
N-acetyl-D-mannosamine
recombinant His-taggged enzyme, pH 7.0, 37°C
160
N-acetyl-D-mannosamine
-
pH 7.0, 37°C
160
N-acetyl-D-mannosamine
-
soluble enzyme, pH not specified in the publication, temperature not specified in the publication
180
N-acetyl-D-mannosamine
-
220
N-acetyl-D-mannosamine
-
245
N-acetyl-D-mannosamine
-
cross-linked enzyme aggregates, pH 7.5, 37°C
272
N-acetyl-D-mannosamine
-
wild type enzyme, at pH 7.0 and 37°C
333
N-acetyl-D-mannosamine
wild type enzyme, at pH 7.0 and 37°C
915
N-acetyl-D-mannosamine
mutant enzyme P193Y, at pH 7.0 and 37°C
3200
N-acetyl-D-mannosamine
mutant enzyme G210S/Y213G, at pH 7.0 and 37°C
2.1
N-acetyl-D-neuraminic acid
-
pH 7.5, 25°C, mutant enzyme Y98H/F115L
2.6
N-acetyl-D-neuraminic acid
-
pH 7.5, 25°C, wild-type enzyme
3.9
N-acetyl-D-neuraminic acid
-
pH 7.5, 25°C, mutant enzyme Y98H/F115L/V251I
4.4
N-acetyl-D-neuraminic acid
-
pH 7.5, 25°C, wild-type enzyme
10.1
N-acetyl-D-neuraminic acid
-
pH 7.5, 25°C, mutant enzyme Y98H/F115L/N153Y/V251I/V265I/Y281C
10.7
N-acetyl-D-neuraminic acid
-
pH 7.5, 25°C, mutant enzyme S208G
12.1
N-acetyl-D-neuraminic acid
-
pH 7.5, 25°C, mutant enzyme Y98H/F115L/V251I/V265I
15.3
N-acetyl-D-neuraminic acid
-
pH 7.5, 25°C, mutant enzyme E60A/Y98H/F115L/N153Y/D150G/V251I/V265I/Y281C/N153Y
17
N-acetyl-D-neuraminic acid
-
pH 7.5, 25°C, mutant enzyme E192S
34
N-acetyl-D-neuraminic acid
-
pH 7.5, 25°C, mutant enzyme E192V
38
N-acetyl-D-neuraminic acid
-
pH 7.5, 25°C, mutant enzyme E192N
3 - 3.5
N-acetyl-L-neuraminic acid
-
pH 7.5, 25°C, mutant enzyme E60A/Y98H/F115L/N153Y/D150G/V251I/V265I/Y281C/N153Y
4.4
N-acetyl-L-neuraminic acid
-
pH 7.5, 25°C, wild-type enzyme
36.2
N-acetyl-L-neuraminic acid
-
pH 7.5, 25°C, mutant enzyme Y98H/F115L/N153Y/V251I/V265I/Y281C
127
N-acetyl-L-neuraminic acid
-
pH 7.5, 25°C, mutant enzyme Y98H/F115L/V251I/V265I
369.4
N-acetyl-L-neuraminic acid
-
pH 7.5, 25°C, mutant enzyme Y98H/F115L/V251I
0.013
N-acetylneuraminate
purified recombinant mutant K165C, pH 7.4, 30°C
0.015
N-acetylneuraminate
purified recombinant mutant K165-gamma-thialysine, pH 6.8, 30°C
0.023
N-acetylneuraminate
purified recombinant mutant K165-gamma-thialysine, pH 7.4, 30°C
0.036
N-acetylneuraminate
purified recombinant wild-type enzyme, pH 7.4, 30°C
0.04
N-acetylneuraminate
purified recombinant wild-type enzyme, pH 6.8, 30°C
0.27
N-acetylneuraminate
mutant enzyme P193Y, at pH 7.0 and 37°C
0.32
N-acetylneuraminate
-
wild type enzyme, at pH 7.0 and 37°C
1.1
N-acetylneuraminate
wild type enzyme, at pH 7.0 and 37°C
1.2
N-acetylneuraminate
mutant enzyme G211S, at pH 7.0 and 37°C
1.8
N-acetylneuraminate
at pH 7.0 and 37°C
2
N-acetylneuraminate
recombinant His-taggged enzyme, pH 7.0, 37°C
2
N-acetylneuraminate
mutant enzyme G210S/Y213G, at pH 7.0 and 37°C
2.2
N-acetylneuraminate
-
wild-type enzyme, pH 7.4, 30°C
2.5
N-acetylneuraminate
-
2.51
N-acetylneuraminate
mutant enzyme L171N/I229N, in sodium phosphate buffer (100 mM, pH 7.6), at 30°C
3 - 3.5
N-acetylneuraminate
at pH 7.5 and 37°C
3.2
N-acetylneuraminate
mutant enzyme L199N/I229D, in sodium phosphate buffer (100 mM, pH 7.6), at 30°C
3.2
N-acetylneuraminate
at 30°C, pH not specified in the publication
3.4
N-acetylneuraminate
mutant enzyme I229N, in sodium phosphate buffer (100 mM, pH 7.6), at 30°C
3.42
N-acetylneuraminate
mutant enzyme L171R, in sodium phosphate buffer (100 mM, pH 7.6), at 30°C
3.7
N-acetylneuraminate
-
mutant Y137F enzyme, pH 7.4, 30°C
3.93
N-acetylneuraminate
mutant enzyme I229R, in sodium phosphate buffer (100 mM, pH 7.6), at 30°C
4
N-acetylneuraminate
mutant enzyme L199N/I229R, in sodium phosphate buffer (100 mM, pH 7.6), at 30°C
4
N-acetylneuraminate
-
recombinant enzyme, pH and temperature not specified in the publication
4.1
N-acetylneuraminate
wild type enzyme, in sodium phosphate buffer (100 mM, pH 7.6), at 30°C
4.3
N-acetylneuraminate
mutant enzyme L199N, in sodium phosphate buffer (100 mM, pH 7.6), at 30°C
4.7
N-acetylneuraminate
mutant enzyme L199N/I229N, in sodium phosphate buffer (100 mM, pH 7.6), at 30°C
4.9
N-acetylneuraminate
-
5
N-acetylneuraminate
mutant enzyme I229D, in sodium phosphate buffer (100 mM, pH 7.6), at 30°C
6.1
N-acetylneuraminate
mutant enzyme L199R/I229N, in sodium phosphate buffer (100 mM, pH 7.6), at 30°C
87.7
N-acetylneuraminate
at pH 8.5 and 37°C
0.4
N-acetylneuraminic acid
-
pH 7.2, Y133F mutant
1
N-acetylneuraminic acid
-
pH 7.2, Y133H mutant
1
N-acetylneuraminic acid
-
pH 7.4, 75°C
1.4
N-acetylneuraminic acid
-
pH 7.4, 37°C
1.5 - 3.7
N-acetylneuraminic acid
-
-
1.7
N-acetylneuraminic acid
-
pH 7.4, 37°C
1.75
N-acetylneuraminic acid
-
-
1.8
N-acetylneuraminic acid
-
-
1.8
N-acetylneuraminic acid
-
pH 7.0, 37°C
1.9 - 3.9
N-acetylneuraminic acid
-
-
2
N-acetylneuraminic acid
-
pH 7.2, 50 mM phosphate buffer, wild-type
2.4
N-acetylneuraminic acid
-
-
2.4
N-acetylneuraminic acid
-
pH 7.2, Y133C mutant
2.8
N-acetylneuraminic acid
-
-
2.9
N-acetylneuraminic acid
-
pH 7.2, Y133W mutant
3.2
N-acetylneuraminic acid
-
pH 7.2, 250 mM phosphate buffer, wild-type
3.3
N-acetylneuraminic acid
-
-
3.3
N-acetylneuraminic acid
-
-
3.5
N-acetylneuraminic acid
-
pH 7.2, K161R mutant
3.5
N-acetylneuraminic acid
-
E192Q mutant protein, pH 7.5, 25°C
3.6
N-acetylneuraminic acid
-
-
3.7
N-acetylneuraminic acid
-
-
3.9
N-acetylneuraminic acid
-
-
4.4
N-acetylneuraminic acid
-
wild type (E192), pH 7.5, 25°C
4.6
N-acetylneuraminic acid
-
E192D mutant protein, pH 7.5, 25°C
4.8
N-acetylneuraminic acid
-
-
5
N-acetylneuraminic acid
-
E192M mutant protein, pH 7.5, 25°C
5.9
N-acetylneuraminic acid
-
E192F mutant protein, pH 7.5, 25°C
6
N-acetylneuraminic acid
-
E192P mutant protein, pH 7.5, 25°C
6
N-acetylneuraminic acid
-
E192S mutant protein, pH 7.5, 25°C
6.2
N-acetylneuraminic acid
-
E192H mutant protein, pH 7.5, 25°C
17
N-acetylneuraminic acid
-
pH 7.2, E188Q mutant
29
N-acetylneuraminic acid
-
pH 7.2, D187E mutant
34
N-acetylneuraminic acid
-
E192V mutant protein, pH 7.5, 25°C
38
N-acetylneuraminic acid
-
E192N mutant protein, pH 7.7.5, 25°C
44
N-acetylneuraminic acid
-
pH 7.2, E188D mutant
1100
N-acetylneuraminic acid
-
pH 7.2, D187N mutant
2.2
N-glycolylneuraminic acid
-
-
3.3
N-glycolylneuraminic acid
-
-
4.3
N-glycolylneuraminic acid
-
-
4.6
N-glycolylneuraminic acid
-
-
0.5
pyruvate
mutant enzyme P193Y, at pH 7.0 and 37°C
1 - 4
pyruvate
recombinant His-taggged enzyme, pH 7.0, 37°C
1.1
pyruvate
wild type enzyme, at pH 7.0 and 37°C
4.3
pyruvate
mutant enzyme G210S/Y213G, at pH 7.0 and 37°C
5.9
pyruvate
at pH 7.0 and 37°C
9
pyruvate
-
cross-linked enzyme aggregates, pH 7.5, 37°C
12.5
pyruvate
-
wild type enzyme, at pH 7.0 and 37°C
14.7
pyruvate
at pH 7.5 and 37°C
19.9
pyruvate
-
pH 7.0, 37°C
19.9
pyruvate
-
soluble enzyme, pH not specified in the publication, temperature not specified in the publication
35.1
pyruvate
-
recombinant enzyme, pH and temperature not specified in the publication
72.4
pyruvate
at pH 8.5 and 37°C
4.4
sialic acid
-
wild-type enzyme
38
sialic acid
-
mutant enzyme E192N
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.057 - 10.83
(2R,3R,4R)-6-[(aminooxy)carbonyl]-3,4,6-trihydroxy-N-propyltetrahydro-2H-pyran-2-carboxamide
0.44 - 2.18
(2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid
0.1 - 2.83
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
1.2 - 8.9
D-3-deoxy-manno-2-octulosonic acid
1.1 - 10.4
L-3-deoxy-manno-2-octulosonic acid
2.4 - 40.67
N-acetyl-D-mannosamine
1.5 - 10.5
N-acetyl-D-neuraminic acid
0.0002 - 0.02
N-acetyl-L-neuraminic acid
0.013 - 44.2
N-acetylneuraminate
0.42 - 16.7
N-acetylneuraminic acid
0.057 - 0.65
(2R,3R,4R)-6-[(aminooxy)carbonyl]-3,4,6-trihydroxy-N-propyltetrahydro-2H-pyran-2-carboxamide
-
mutant enzyme E192N
1.23
(2R,3R,4R)-6-[(aminooxy)carbonyl]-3,4,6-trihydroxy-N-propyltetrahydro-2H-pyran-2-carboxamide
-
wild-type enzyme
10.83
(2R,3R,4R)-6-[(aminooxy)carbonyl]-3,4,6-trihydroxy-N-propyltetrahydro-2H-pyran-2-carboxamide
-
mutant enzyme E192N
0.44
(2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid
-
pH 7.5, 25°C, mutant enzyme S208G
0.91
(2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid
-
pH 7.5, 25°C, mutant enzyme D191P
1.22
(2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid
-
pH 7.5, 25°C, wild-type enzyme
1.58
(2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid
-
pH 7.5, 25°C, mutant enzyme E192V
1.85
(2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid
-
pH 7.5, 25°C, mutant enzyme E192S
2.18
(2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid
-
pH 7.5, 25°C, mutant enzyme E192N
0.1
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
-
E192D mutant protein, pH 7.5, 25°C
0.43
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
-
E192S mutant protein, pH 7.5, 25°C
0.63
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
-
E192H mutant protein, pH 7.5, 25°C
0.82
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
-
E192P mutant protein, pH 7.5, 25°C
1.17
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
-
E192M mutant protein, pH 7.5, 25°C
1.22
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
-
wild type (E192), pH 7.5, 25°C
1.47
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
-
E192F mutant protein, pH 7.5, 25°C
1.58
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
-
E192V mutant protein, pH 7.5, 25°C
2.17
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
-
E192N mutant protein, pH 7.7.5, 25°C
2.83
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
-
E192Q mutant protein, pH 7.5, 25°C
1.2
D-3-deoxy-manno-2-octulosonic acid
-
pH 7.5, 25°C, wild-type enzyme
1.5
D-3-deoxy-manno-2-octulosonic acid
-
pH 7.5, 25°C, mutant enzyme Y98H/F115L
2.8
D-3-deoxy-manno-2-octulosonic acid
-
pH 7.5, 25°C, mutant enzyme Y98H/F115L/V251I
3.2
D-3-deoxy-manno-2-octulosonic acid
-
pH 7.5, 25°C, mutant enzyme Y98H/F115L/V251I/V265I
5.1
D-3-deoxy-manno-2-octulosonic acid
-
pH 7.5, 25°C, mutant enzyme Y98H/F115L/N153Y/V251I/V265I/Y281C
8.9
D-3-deoxy-manno-2-octulosonic acid
-
pH 7.5, 25°C, mutant enzyme E60A/Y98H/F115L/N153Y/D150G/V251I/V265I/Y281C/N153Y
1.1
L-3-deoxy-manno-2-octulosonic acid
-
pH 7.5, 25°C, wild-type enzyme
1.1
L-3-deoxy-manno-2-octulosonic acid
-
pH 7.5, 25°C, mutant enzyme Y98H/F115L
1.9
L-3-deoxy-manno-2-octulosonic acid
-
pH 7.5, 25°C, mutant enzyme Y98H/F115L/V251I
2.5
L-3-deoxy-manno-2-octulosonic acid
-
pH 7.5, 25°C, mutant enzyme Y98H/F115L/V251I/V265I
4.2
L-3-deoxy-manno-2-octulosonic acid
-
pH 7.5, 25°C, mutant enzyme Y98H/F115L/N153Y/V251I/V265I/Y281C
10.4
L-3-deoxy-manno-2-octulosonic acid
-
pH 7.5, 25°C, mutant enzyme E60A/Y98H/F115L/N153Y/D150G/V251I/V265I/Y281C/N153Y
2.4
N-acetyl-D-mannosamine
mutant enzyme P193Y, at pH 7.0 and 37°C
4.5
N-acetyl-D-mannosamine
recombinant His-taggged enzyme, pH 7.0, 37°C
4.6
N-acetyl-D-mannosamine
-
wild type enzyme, at pH 7.0 and 37°C
5.4
N-acetyl-D-mannosamine
wild type enzyme, at pH 7.0 and 37°C
5.67
N-acetyl-D-mannosamine
at pH 7.5 and 37°C
9
N-acetyl-D-mannosamine
-
11
N-acetyl-D-mannosamine
-
15.3
N-acetyl-D-mannosamine
mutant enzyme G210S/Y213G, at pH 7.0 and 37°C
40.67
N-acetyl-D-mannosamine
at pH 8.5 and 37°C
1.5
N-acetyl-D-neuraminic acid
-
pH 7.5, 25°C, mutant enzyme E60A/Y98H/F115L/N153Y/D150G/V251I/V265I/Y281C/N153Y
1.7
N-acetyl-D-neuraminic acid
-
pH 7.5, 25°C, mutant enzyme Y98H/F115L/N153Y/V251I/V265I/Y281C
1.97
N-acetyl-D-neuraminic acid
-
pH 7.5, 25°C, mutant enzyme E192V
2.2
N-acetyl-D-neuraminic acid
-
pH 7.5, 25°C, mutant enzyme Y98H/F115L/V251I/V265I
2.77
N-acetyl-D-neuraminic acid
-
pH 7.5, 25°C, mutant enzyme E192N
4.15
N-acetyl-D-neuraminic acid
-
pH 7.5, 25°C, mutant enzyme S208G
4.25
N-acetyl-D-neuraminic acid
-
pH 7.5, 25°C, mutant enzyme E192S
4.3
N-acetyl-D-neuraminic acid
-
pH 7.5, 25°C, wild-type enzyme
9.7
N-acetyl-D-neuraminic acid
-
pH 7.5, 25°C, mutant enzyme Y98H/F115L/V251I
10.3
N-acetyl-D-neuraminic acid
-
pH 7.5, 25°C, Y98H/F115L
10.5
N-acetyl-D-neuraminic acid
-
pH 7.5, 25°C, wild-type enzyme
0.0002
N-acetyl-L-neuraminic acid
-
pH 7.5, 25°C, wild-type enzyme
0.0045
N-acetyl-L-neuraminic acid
-
pH 7.5, 25°C, mutant enzyme Y98H/F115L/V251I
0.0068
N-acetyl-L-neuraminic acid
-
pH 7.5, 25°C, mutant enzyme Y98H/F115L/V251I/V265I
0.02
N-acetyl-L-neuraminic acid
-
pH 7.5, 25°C, mutant enzyme E60A/Y98H/F115L/N153Y/D150G/V251I/V265I/Y281C/N153Y
0.02
N-acetyl-L-neuraminic acid
-
pH 7.5, 25°C, mutant enzyme Y98H/F115L/N153Y/V251I/V265I/Y281C
0.013
N-acetylneuraminate
-
mutant Y137F enzyme, pH 7.4, 30°C
0.013
N-acetylneuraminate
purified recombinant mutant K165C, pH 7.4, 30°C
0.015
N-acetylneuraminate
purified recombinant mutant K165-gamma-thialysine, pH 6.8, 30°C
0.023
N-acetylneuraminate
purified recombinant mutant K165-gamma-thialysine, pH 7.4, 30°C
0.032
N-acetylneuraminate
mutant enzyme G211S, at pH 7.0 and 37°C
0.036
N-acetylneuraminate
purified recombinant wild-type enzyme, pH 7.4, 30°C
0.04
N-acetylneuraminate
purified recombinant wild-type enzyme, pH 6.8, 30°C
0.13
N-acetylneuraminate
mutant enzyme P193Y, at pH 7.0 and 37°C
0.29
N-acetylneuraminate
mutant enzyme L199N/I229D, in sodium phosphate buffer (100 mM, pH 7.6), at 30°C
0.47
N-acetylneuraminate
mutant enzyme G210S/Y213G, at pH 7.0 and 37°C
0.664
N-acetylneuraminate
mutant enzyme L199N/I229N, in sodium phosphate buffer (100 mM, pH 7.6), at 30°C
0.9
N-acetylneuraminate
-
wild type enzyme, at pH 7.0 and 37°C
1.9
N-acetylneuraminate
wild type enzyme, at pH 7.0 and 37°C
3.15
N-acetylneuraminate
mutant enzyme L171N/I229N, in sodium phosphate buffer (100 mM, pH 7.6), at 30°C
4
N-acetylneuraminate
recombinant His-taggged enzyme, pH 7.0, 37°C
4.17
N-acetylneuraminate
-
wild-type enzyme, pH 7.4, 30°C
4.69
N-acetylneuraminate
mutant enzyme I229D, in sodium phosphate buffer (100 mM, pH 7.6), at 30°C
5.49
N-acetylneuraminate
mutant enzyme L171R, in sodium phosphate buffer (100 mM, pH 7.6), at 30°C
6.75
N-acetylneuraminate
wild type enzyme, in sodium phosphate buffer (100 mM, pH 7.6), at 30°C
7.4
N-acetylneuraminate
mutant enzyme I229N, in sodium phosphate buffer (100 mM, pH 7.6), at 30°C
7.72
N-acetylneuraminate
mutant enzyme I229R, in sodium phosphate buffer (100 mM, pH 7.6), at 30°C
7.94
N-acetylneuraminate
mutant enzyme L199N, in sodium phosphate buffer (100 mM, pH 7.6), at 30°C
8.9
N-acetylneuraminate
mutant enzyme L199N/I229R, in sodium phosphate buffer (100 mM, pH 7.6), at 30°C
9.3
N-acetylneuraminate
at pH 7.5 and 37°C
11.87
N-acetylneuraminate
mutant enzyme L199R/I229N, in sodium phosphate buffer (100 mM, pH 7.6), at 30°C
22.1
N-acetylneuraminate
at 30°C, pH not specified in the publication
44.2
N-acetylneuraminate
at pH 8.5 and 37°C
0.42
N-acetylneuraminic acid
-
E192P mutant protein, pH 7.5, 25°C
0.83
N-acetylneuraminic acid
-
E192S mutant protein, pH 7.5, 25°C
1.17
N-acetylneuraminic acid
-
E192M mutant protein, pH 7.5, 25°C
2
N-acetylneuraminic acid
-
E192D mutant protein, pH 7.5, 25°C
2
N-acetylneuraminic acid
-
E192H mutant protein, pH 7.5, 25°C
2
N-acetylneuraminic acid
-
E192V mutant protein, pH 7.5, 25°C
2.67
N-acetylneuraminic acid
-
E192F mutant protein, pH 7.5, 25°C
2.83
N-acetylneuraminic acid
-
E192N mutant protein, pH 7.7.5, 25°C
4.33
N-acetylneuraminic acid
-
wild type (E192), pH 7.5, 25°C
5
N-acetylneuraminic acid
-
E192Q mutant protein, pH 7.5, 25°C
6.8
N-acetylneuraminic acid
pH 7.2, 25°C, L142R/Y190D/E192A mutant
7.7
N-acetylneuraminic acid
pH 7.2, 25°C, wild-type
12.1
N-acetylneuraminic acid
pH 7.2, 25°C, L142R mutant
16.7
N-acetylneuraminic acid
-
-
0.99
pyruvate
mutant enzyme P193Y, at pH 7.0 and 37°C
2.9
pyruvate
recombinant His-taggged enzyme, pH 7.0, 37°C
3
pyruvate
mutant enzyme G210S/Y213G, at pH 7.0 and 37°C
3.9
pyruvate
-
wild type enzyme, at pH 7.0 and 37°C
5.6
pyruvate
wild type enzyme, at pH 7.0 and 37°C
6.1
pyruvate
at pH 7.5 and 37°C
42.58
pyruvate
at pH 8.5 and 37°C
2.833
sialic acid
-
mutant enzyme E192N
3 - 6
sialic acid
-
mutant enzyme E192N
4.33
sialic acid
-
wild-type enzyme
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0.08 - 5.67
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
0.002 - 1.86
N-acetyl-D-mannosamine
0.0033 - 23.75
N-acetylneuraminate
0.06 - 5.6
N-acetylneuraminic acid
0.08
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
-
E192D mutant protein, pH 7.5, 25°C
0.12
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
-
wild type (E192), pH 7.5, 25°C
0.7
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
-
E192S mutant protein, pH 7.5, 25°C
1.33
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
-
E192H mutant protein, pH 7.5, 25°C
4.17
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
-
E192Q mutant protein, pH 7.5, 25°C
4.33
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
-
E192P mutant protein, pH 7.5, 25°C
4.5
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
-
E192V mutant protein, pH 7.5, 25°C
5.17
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
-
E192F mutant protein, pH 7.5, 25°C
5.33
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
-
E192M mutant protein, pH 7.5, 25°C
5.67
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
-
E192N mutant protein, pH 7.7.5, 25°C
0.002
N-acetyl-D-mannosamine
mutant enzyme P193Y, at pH 7.0 and 37°C
0.004
N-acetyl-D-mannosamine
mutant enzyme G210S/Y213G, at pH 7.0 and 37°C
0.0074
N-acetyl-D-mannosamine
-
cross-linked enzyme aggregates, pH 7.5, 37°C
0.016
N-acetyl-D-mannosamine
-
wild type enzyme, at pH 7.0 and 37°C
0.016
N-acetyl-D-mannosamine
wild type enzyme, at pH 7.0 and 37°C
0.03
N-acetyl-D-mannosamine
-
pH 7.0, 37°C
0.03
N-acetyl-D-mannosamine
-
soluble enzyme, pH not specified in the publication, temperature not specified in the publication
0.03
N-acetyl-D-mannosamine
recombinant His-taggged enzyme, pH 7.0, 37°C
0.07
N-acetyl-D-mannosamine
at pH 8.5 and 37°C
0.08
N-acetyl-D-mannosamine
-
recombinant enzyme, pH and temperature not specified in the publication
0.11
N-acetyl-D-mannosamine
at pH 7.5 and 37°C
1.86
N-acetyl-D-mannosamine
at pH 7.0 and 37°C
0.0033
N-acetylneuraminate
-
mutant Y137F enzyme, pH 7.4, 30°C
0.026
N-acetylneuraminate
mutant enzyme G211S, at pH 7.0 and 37°C
0.23
N-acetylneuraminate
mutant enzyme G210S/Y213G, at pH 7.0 and 37°C
0.28
N-acetylneuraminate
at pH 7.5 and 37°C
0.48
N-acetylneuraminate
mutant enzyme P193Y, at pH 7.0 and 37°C
1.7
N-acetylneuraminate
wild type enzyme, at pH 7.0 and 37°C
1.9
N-acetylneuraminate
-
wild-type enzyme, pH 7.4, 30°C
1.9
N-acetylneuraminate
-
recombinant enzyme, pH and temperature not specified in the publication
2
N-acetylneuraminate
recombinant His-taggged enzyme, pH 7.0, 37°C
2.8
N-acetylneuraminate
-
wild type enzyme, at pH 7.0 and 37°C
3.66
N-acetylneuraminate
at pH 7.0 and 37°C
23.75
N-acetylneuraminate
at pH 8.5 and 37°C
0.06
N-acetylneuraminic acid
-
E192V mutant protein, pH 7.5, 25°C
0.07
N-acetylneuraminic acid
-
E192N mutant protein, pH 7.7.5, 25°C
0.07
N-acetylneuraminic acid
-
E192P mutant protein, pH 7.5, 25°C
0.13
N-acetylneuraminic acid
-
E192S mutant protein, pH 7.5, 25°C
0.23
N-acetylneuraminic acid
-
E192M mutant protein, pH 7.5, 25°C
0.32
N-acetylneuraminic acid
-
E192H mutant protein, pH 7.5, 25°C
0.43
N-acetylneuraminic acid
-
E192D mutant protein, pH 7.5, 25°C
0.45
N-acetylneuraminic acid
-
E192F mutant protein, pH 7.5, 25°C
0.98
N-acetylneuraminic acid
-
wild type (E192), pH 7.5, 25°C
1.42
N-acetylneuraminic acid
-
E192Q mutant protein, pH 7.5, 25°C
5.6
N-acetylneuraminic acid
-
pH 7.0, 37°C
0.08
pyruvate
-
recombinant enzyme, pH and temperature not specified in the publication
0.09
pyruvate
-
cross-linked enzyme aggregates, pH 7.5, 37°C
0.11
pyruvate
-
pH 7.0, 37°C
0.11
pyruvate
-
soluble enzyme, pH not specified in the publication, temperature not specified in the publication
0.11
pyruvate
at pH 8.5 and 37°C
0.21
pyruvate
recombinant His-taggged enzyme, pH 7.0, 37°C
0.31
pyruvate
-
wild type enzyme, at pH 7.0 and 37°C
0.41
pyruvate
at pH 7.5 and 37°C
0.71
pyruvate
mutant enzyme G210S/Y213G, at pH 7.0 and 37°C
1.12
pyruvate
at pH 7.0 and 37°C
1.98
pyruvate
mutant enzyme P193Y, at pH 7.0 and 37°C
5.1
pyruvate
wild type enzyme, at pH 7.0 and 37°C
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D187N
-
decreased Vmax, increased Km
E188D
-
cleaves 5-N-acetyl-9-O-acetylneuraminic acid as good as N-acetylneuraminic acid
E188Q
-
cleaves 5-N-acetyl-9-O-acetylneuraminic acid as good as N-acetylneuraminic acid
Y133C
-
significant decrease in enzyme activity
Y133F
-
significant decrease in enzyme activity
Y133H
-
significant decrease in enzyme activity
Y133W
-
significant decrease in enzyme activity
D191P
-
ratio of turnover-number to KM-value for (2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid is 1.4fold higher than the wild-type ratio
E192A
-
strong interaction of Glu192 (wild type) with hydroxyl groups at C8 and C9 of N-acetylneuraminic acid
E192C
-
strong interaction of Glu192 (wild type) with hydroxyl groups at C8 and C9 of N-acetylneuraminic acid
E192D
-
strong interaction of Glu192 (wild type) with hydroxyl groups at C8 and C9 of N-acetylneuraminic acid
E192F
-
strong interaction of Glu192 (wild type) with hydroxyl groups at C8 and C9 of N-acetylneuraminic acid
E192G
-
strong interaction of Glu192 (wild type) with hydroxyl groups at C8 and C9 of N-acetylneuraminic acid
E192H
-
strong interaction of Glu192 (wild type) with hydroxyl groups at C8 and C9 of N-acetylneuraminic acid
E192I
-
strong interaction of Glu192 (wild type) with hydroxyl groups at C8 and C9 of N-acetylneuraminic acid
E192K
-
strong interaction of Glu192 (wild type) with hydroxyl groups at C8 and C9 of N-acetylneuraminic acid
E192L
-
strong interaction of Glu192 (wild type) with hydroxyl groups at C8 and C9 of N-acetylneuraminic acid
E192M
-
strong interaction of Glu192 (wild type) with hydroxyl groups at C8 and C9 of N-acetylneuraminic acid
E192P
-
strong interaction of Glu192 (wild type) with hydroxyl groups at C8 and C9 of N-acetylneuraminic acid
E192Q
-
strong interaction of Glu192 (wild type) with hydroxyl groups at C8 and C9 of N-acetylneuraminic acid
E192R
-
strong interaction of Glu192 (wild type) with hydroxyl groups at C8 and C9 of N-acetylneuraminic acid
E192T
-
strong interaction of Glu192 (wild type) with hydroxyl groups at C8 and C9 of N-acetylneuraminic acid
E192W
-
strong interaction of Glu192 (wild type) with hydroxyl groups at C8 and C9 of N-acetylneuraminic acid
E192Y
-
strong interaction of Glu192 (wild type) with hydroxyl groups at C8 and C9 of N-acetylneuraminic acid
E60A/Y98H/F115L/N153Y/D150G/V251I/V265I/Y281C/N153Y
-
KM-value for N-acetyl-D-neuraminic acid is 5.9fold higher than the wild-type value, turnover-number for N-acetyl-D-neuraminic acid is 14% of the wild-type value, KM-value for N-acetyl-L-neuraminic acid is 7.6fold higher than the wild-type value, turnover-number for N-acetyl-L-neuraminic acid is 100fold higher than the wild-type value, KM-value for D-3-deoxy-manno-2-octulosonic acid is 83% of the wild-type value, turnover-number for D-3-deoxy-manno-2-octulosonic acid is 7.4fold higher than wild-type value, KM-value for L-3-deoxy-manno-2-octulosonic acid is 37% of the wild-type value, turnover-number for L-3-deoxy-manno-2-octulosonic acid is 9.5fold higher than wild-type value. kcat/KM for N-acetyl-D-neuraminic acid is 40.4fold lower than the wild-type value, kcat/KM for N-acetyl-D-neuraminic acid is 20fold higher than the wild-type value, kcat/KM for L-3-deoxy-manno-2-octulosonic acid is 24.7fold higher than the wild-type value
I229D
the mutant shows decreased kcat compared to the wild type enzyme
I229N
the mutant shows increased kcat compared to the wild type enzyme
I229R
the mutant shows increased kcat compared to the wild type enzyme
L142R
increased activity towards L-aspartate-beta-semialdehyde
L142R/Y190D/E192A
less efficient than wild-type enzyme
L171D
the mutant is insoluble
L171D/L199D/I229D
the mutant is insoluble
L171D/L199N/I229D
the mutant is insoluble
L171N
the mutant is insoluble
L171N/I229N
the mutant shows decreased kcat compared to the wild type enzyme
L171R
the mutant shows decreased kcat compared to the wild type enzyme
L171R/L199N/I229R
the mutant is insoluble
L171R/L199R/I229R
the mutant is insoluble
L199D
the mutant is insoluble
L199N
the mutant shows increased kcat compared to the wild type enzyme
L199N/I229D
the mutant shows decreased kcat compared to the wild type enzyme
L199N/I229N
the mutant shows decreased kcat compared to the wild type enzyme
L199N/I229R
the mutant shows increased kcat compared to the wild type enzyme
L199R
the mutant is insoluble
L199R/I229N
the mutant shows increased kcat compared to the wild type enzyme
S208G
-
ratio of turnover-number to KM-value for N-acetyl-D-neuraminic acid is 2.5fold lower than the wild-type ratio, ratio of turnover-number to KM-value for (2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid is 1.5fold higher than the wild-type ratio
Y98H/F115L
-
KM-value for N-acetyl-D-neuraminic acid is 81% of the wild-type value, turnover-number for N-acetyl-D-neuraminic acid isnearly identical to wild-type value, KM-value for D-3-deoxy-manno-2-octulosonic acid is 89% of the wild-type value, turnover-number for D-3-deoxy-manno-2-octulosonic acid is 1.25fold higher than wild-type value, KM-value for L-3-deoxy-manno-2-octulosonic acid is 1.9fold higher than the wild-type value, turnover-number for L-3-deoxy-manno-2-octulosonic acid is identical to wild-type value. kcat/KM for N-acetyl-D-neuraminic acid is 1.1fold higher than the wild-type ratio, kcat/Km for D-3-deoxy-manno-2-octulosonic acid is 1.4fold higher than the wild-type ratio, kcat/KM for L-3-deoxy-manno-2-octulosonic acid is 1.8fold lower than the wild-type ratio
Y98H/F115L/N153Y/V251I/V265I/Y281C
-
KM-value for N-acetyl-D-neuraminic acid is 3.9fold higher than the wild-type value, turnover-number for N-acetyl-D-neuraminic acid is 16% of the wild-type value, KM-value for N-acetyl-L-neuraminic acid is 2.6fold higher than the wild-type value, turnover-number for N-acetyl-L-neuraminic acid is 100fold higher than the wild-type value, KM-value for D-3-deoxy-manno-2-octulosonic acid is 46% of the wild-type value, turnover-number for D-3-deoxy-manno-2-octulosonic acid is 4.36fold higher than wild-type value, KM-value for L-3-deoxy-manno-2-octulosonic acid is 32% of the wild-type value, turnover-number for L-3-deoxy-manno-2-octulosonic acid is 3.8fold higher than wild-type value. kcat/Km for N-acetyl-D-neuraminic acid is 23.8fold lower than the wild-type value, kcat/Km for N-acetyl-D-neuraminic acid is 20fold higher than the wild-type value, kcat/Km for D-3-deoxy-manno-2-octulosonic acid is 9.3fold higher than the wild-type value
Y98H/F115L/V251I
-
KM-value for N-acetyl-D-neuraminic acid is 1.5fold higher than the wild-type value, turnover-number for N-acetyl-D-neuraminic acid is 92% of the wild-type value, KM-value for N-acetyl-L-neuraminic acid is 84fold higher than the wild-type value, turnover-number for N-acetyl-L-neuraminic acid is 22.5fold higher than the wild-type value, KM-value for D-3-deoxy-manno-2-octulosonic acid is nearly identical to the wild-type value, turnover-number for D-3-deoxy-manno-2-octulosonic acid is 2.3fold higher than wild-type value, KM-value for L-3-deoxy-manno-2-octulosonic acid is 1.2fold higher than the wild-type value, turnover-number for L-3-deoxy-manno-2-octulosonic acid is 1.7fold higher than wild-type value. kcat/KM for N-acetyl-D-neuraminic acid is 1.6fold lower than the wild-type value, kcat/KM for N-acetyl-D-neuraminic acid is 5fold lower than the wild-type value, kcat/KM for D-3-deoxy-manno-2-octulosonic acid is 2.2fold higher than the wild-type value, kcat/KM for L-3-deoxy-manno-2-octulosonic acid is 1.4fold higher than the wild-type value
Y98H/F115L/V251I/V265I
-
KM-value for N-acetyl-D-neuraminic acid is 4.5fold higher than the wild-type value, turnover-number for N-acetyl-D-neuraminic acid is 45% of the wild-type value, KM-value for N-acetyl-L-neuraminic acid is 28.9fold higher than the wild-type value, turnover-number for N-acetyl-L-neuraminic acid is 34fold higher than the wild-type value, KM-value for D-3-deoxy-manno-2-octulosonic acid is 25% of the wild-type value, turnover-number for D-3-deoxy-manno-2-octulosonic acid is 2.6fold higher than wild-type value, KM-value for L-3-deoxy-manno-2-octulosonic acid is 36% of the wild-type value, turnover-number for L-3-deoxy-manno-2-octulosonic acid is 2.3fold higher than wild-type value. kcat/Km for N-acetyl-D-neuraminic acid is 22.4fold lower than the wild-type value, kcat/Km for N-acetyl-D-neuraminic acid is 2fold higher than the wild-type value
L171N/I229N
-
the mutant shows decreased kcat compared to the wild type enzyme
-
L199N/I229D
-
the mutant shows decreased kcat compared to the wild type enzyme
-
L199N/I229N
-
the mutant shows decreased kcat compared to the wild type enzyme
-
L199N/I229R
-
the mutant shows increased kcat compared to the wild type enzyme
-
L199R/I229N
-
the mutant shows increased kcat compared to the wild type enzyme
-
E192N
-
the turnover-number for sialic acid is 65% of the wild-type value, the Km-value for sialic acid is 8.6fold higher than the wild-type value, the turnover-number for (2R,3R,4R)-6-[(aminooxy)carbonyl]-3,4,6-trihydroxy-N-propyltetrahydro-2H-pyran-2-carboxamide is 57% of the wild-type value, the KM-value for (2R,3R,4R)-6-[(aminooxy)carbonyl]-3,4,6-trihydroxy-N-propyltetrahydro-2H-pyran-2-carboxamide is 28.2fold lower than the wild-type value. The mutant enzyme is most efficient in catalysis of the synthesis of the tertiary amides (4S,5R,6R)-6-[(diethylamino)carbonyl]-2,4,5-trihydroxytetrahydro-2H-pyran-2-carboxylic acid, (4R,5R,6R)-6-[(diethylamino)carbonyl]-2,4,5-trihydroxytetrahydro-2H-pyran-2-carboxylic acid, (4S,5R,6R)-2,4,5-trihydroxy-6-{[methyl(propyl)amino]carbonyl}tetrahydro-2H-pyran-2-carboxylic acid, (4R,5R,6R)-2,4,5-trihydroxy-6-{[methyl(propyl)amino]carbonyl}tetrahydro-2H-pyran-2-carboxylic acid, (4S,5R,6R)-6-[(dipropylamino)carbonyl]-2,4,5-trihydroxytetrahydro-2H-pyran-2-carboxylic acid, (4R,5R,6R)-6-[(dipropylamino)carbonyl]-2,4,5-trihydroxytetrahydro-2H-pyran-2-carboxylic acid, (4S,5R,6R)-6-[(dibutylamino)carbonyl]-2,4,5-trihydroxytetrahydro-2H-pyran-2-carboxylic acid, (4R,5R,6R)-6-[(dibutylamino)carbonyl]-2,4,5-trihydroxytetrahydro-2H-pyran-2-carboxylic acid, (4S,5R,6R)-2,4,5-trihydroxy-6-(pyrrolidin-1-ylcarbonyl)tetrahydro-2H-pyran-2-carboxylic acid, (4R,5R,6R)-2,4,5-trihydroxy-6-(pyrrolidin-1-ylcarbonyl)tetrahydro-2H-pyran-2-carboxylic acid, (4S,5R,6R)-2,4,5-trihydroxy-6-(piperidin-1-ylcarbonyl)tetrahydro-2H-pyran-2-carboxylic acid, (4R,5R,6R)-2,4,5-trihydroxy-6-(piperidin-1-ylcarbonyl)tetrahydro-2H-pyran-2-carboxylic acid, (4S,5R,6R)-2,4,5-trihydroxy-6-(morpholin-4-ylcarbonyl)tetrahydro-2H-pyran-2-carboxylic acid, (4R,5R,6R)-2,4,5-trihydroxy-6-(morpholin-4-ylcarbonyl)tetrahydro-2H-pyran-2-carboxylic acid
F252A
-
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
F252Y
-
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
S47A
-
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme
S47C
-
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme
S47T
-
site-directed mutagenesis, the mutant shows slightly increased activity compared to the wild-type enzyme
T167A
-
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
T167S
-
site-directed mutagenesis, the mutant shows unaltered activity compared to the wild-type enzyme
T48A
-
site-directed mutagenesis, the mutant shows increased activity compared to the wild-type enzyme
T48S
-
site-directed mutagenesis, the mutant shows increased activity compared to the wild-type enzyme
Y110A
-
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme
Y110F
-
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
Y137A
-
site-directed mutagenesis, inactive mutant
Y137F
-
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme
G210S/Y213G
the mutant shows reduced activity compared to the wild type enzyme
G211S
the mutant shows reduced activity compared to the wild type enzyme
P193Y
the mutant shows reduced activity compared to the wild type enzyme
G210S/Y213G
-
the mutant shows reduced activity compared to the wild type enzyme
-
G211S
-
the mutant shows reduced activity compared to the wild type enzyme
-
P193Y
-
the mutant shows reduced activity compared to the wild type enzyme
-
W189A
the kcat/Km value of the mutant is about 3fold higher than those of the wild type enzyme
W189F
the kcat/Km value of the mutant is about 10fold higher than those of the wild type enzyme
W189Y
the kcat/Km value of the mutant is about 7fold higher than those of the wild type enzyme
K164A
site-directed mutagenesis, determiniation of three structures of the K164A variant: one in the absence of substrates and two binary complexes with N-acetylneuraminic acid and N-glycolylneuraminic acid. Both sialic acids bind to the active site in the open-chain ketone form of the monosaccharide, every hydroxyl group of the linear sugars makes hydrogen bond interactions with the enzyme
C118A
site-directed mutagenesis, the mutant shows kinetic properties identical to the wild-type enzyme
C118S
site-directed mutagenesis, the mutant shows kinetic properties identical to the wild-type enzyme
C238A
site-directed mutagenesis, the mutant shows kinetic properties identical to the wild-type enzyme
C238S
site-directed mutagenesis, the mutant shows kinetic properties identical to the wild-type enzyme
C270A
site-directed mutagenesis, the mutant shows kinetic properties identical to the wild-type enzyme
C270S
site-directed mutagenesis, the mutant shows kinetic properties identical to the wild-type enzyme
C82A
site-directed mutagenesis, the mutant shows kinetic properties identical to the wild-type enzyme
C82S
site-directed mutagenesis, the mutant shows kinetic properties identical to the wild-type enzyme
K165C
site-directed mutagenesis to introduce a cysteine in place of Lys165 in the enzyme active site and complete conversion of the cysteine into gamma-thialysine through dehydroalanine as by chemical mutagenesis, ESI-mass spectrometry and kinetic characterisation, the K165C variant is severely impaired in catalysis, kcat/Km is reduced 720fold compared with wild-type
K165Dha
site-directed mutagenesis to introduce a cysteine in place of Lys165 in the enzyme active site and complete conversion of the cysteine into gamma-thialysine, Dha, through dehydroalanine as by chemical mutagenesis, ESI-mass spectrometry and kinetic characterisation, the enzyme containing gamma-thialysine regains 17-30% of the wild-type activity
C238S
-
site-directed mutagenesis, the mutant shows kinetic properties identical to the wild-type enzyme
-
C270S
-
site-directed mutagenesis, the mutant shows kinetic properties identical to the wild-type enzyme
-
C82A
-
site-directed mutagenesis, the mutant shows kinetic properties identical to the wild-type enzyme
-
C82S
-
site-directed mutagenesis, the mutant shows kinetic properties identical to the wild-type enzyme
-
K165C
-
site-directed mutagenesis to introduce a cysteine in place of Lys165 in the enzyme active site and complete conversion of the cysteine into gamma-thialysine through dehydroalanine as by chemical mutagenesis, ESI-mass spectrometry and kinetic characterisation, the K165C variant is severely impaired in catalysis, kcat/Km is reduced 720fold compared with wild-type
-
E192N
-
ratio of turnover-number to KM-value for N-acetyl-D-neuraminic acid is 13.4fold lower than the wild-type ratio, ratio of turnover-number to KM-value for (2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid is 48.7fold higher than the wild-type ratio
E192N
the mutant has a 6fold higher specificity (kcat/Km) for dipropylaminocarbonyl-substituted derivatives than wild type enzyme
E192N
-
strong interaction of Glu192 (wild type) with hydroxyl groups at C8 and C9 of N-acetylneuraminic acid
E192S
-
ratio of turnover-number to KM-value for N-acetyl-D-neuraminic acid is 3.9fold lower than the wild-type ratio, ratio of turnover-number to KM-value for (2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid is 32.2fold higher than the wild-type ratio
E192S
-
strong interaction of Glu192 (wild type) with hydroxyl groups at C8 and C9 of N-acetylneuraminic acid
E192V
-
ratio of turnover-number to KM-value for N-acetyl-D-neuraminic acid is 17.9fold lower than the wild-type ratio, ratio of turnover-number to KM-value for (2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid is 39.3fold higher than the wild-type ratio
E192V
-
strong interaction of Glu192 (wild type) with hydroxyl groups at C8 and C9 of N-acetylneuraminic acid
additional information
-
directed evolution of D-sialic acid aldolase to L-3-deoxy-manno-2-octulosonic acid (L-KDO) aldolase
additional information
-
synthesis of N-acetylneuraminic acid from N-acetylglucosamine and pyruvate by constructing and co-expressing a polycistronic plasmid encoding an N-acetylglucosamine 2-epimerase gene from Synechocystis sp. PCC 6803 (snAGE) or Anabaena sp. CH1 (anAGE) and the Escherichia coli N-acetylneuraminic acid lyase gene. Construction of four polycistronic plasmids in which the positions of AGE gene differ with respect to Nal gene, plasmid pET-28a-Nal-anAGE with anAGE gene located next to Nal gene produces the highest amount of N-acetylneuraminic acid, overview. anAGE from Anabaena sp. CH1 shows much higher activity and productivity than snAGE from Synechocystis sp. PCC 6803
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45
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