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IUBMB Comments(1R,2S)-1-hydroxypropane-1,2,3-tricarboxylate is the commonly occurring isomer of isocitrate.
The expected taxonomic range for this enzyme is: Talaromyces purpureogenus
Synonyms
Epimerase, isocitrate,
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Epimerase, isocitrate
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(1R,2S)-1-hydroxypropane-1,2,3-tricarboxylate = (1S,2S)-1-hydroxypropane-1,2,3-tricarboxylate
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(1R,2S)-1-hydroxypropane-1,2,3-tricarboxylate 1-epimerase
(1R,2S)-1-hydroxypropane-1,2,3-tricarboxylate is the commonly occurring isomer of isocitrate.
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(1R,2S)-1-Hydroxypropane-1,2,3-tricarboxylate
(1S, 2S)-1-Hydroxypropane-1,2,3-tricarboxylate
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i.e. threo-D-isocitrate
i.e. erythro-L-isocitrate
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pyridoxal 5'-phosphate
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slight stimulation
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4.7 - 5.6
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synthesis of erythro-isocitrate
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3 - 8
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3: about 35% of maximal activity, 8: about 30% of maximal activity, synthesis of erythro-isocitrate
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brenda
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brenda
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20
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room temperature, half life: 12 h, with 30% glycerol, and 1 mM DTT, half-life: less than 3 h, without stabilizers
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30% v/v glycerol plus 1 mM DTT stabilize against heat inactivation, slight protection by leupeptin, pepstatin, and PMSF
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Hoshiko, S.; Kunimoto, Y.; Arima, K.; Beppu, T.
Mechanism of L-alloisocitric acid fermentation: isocitrate epimerase activity in the cell-free extract of Penicillium purpurogenum
Agric. Biol. Chem.
46
143-151
1982
Talaromyces purpureogenus
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brenda
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