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ATP + L-Asp + hydroxylamine
AMP + diphosphate + Asn + beta-aspartyl hydroxamate
ATP + L-Asp + NH4+
AMP + diphosphate + Asn
ATP + L-aspartate + glutamine
?
ATP + L-aspartate + NH3
AMP + diphosphate + L-asparagine
additional information
?
-
ATP + L-Asp + hydroxylamine
AMP + diphosphate + Asn + beta-aspartyl hydroxamate
-
-
-
?
ATP + L-Asp + hydroxylamine
AMP + diphosphate + Asn + beta-aspartyl hydroxamate
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hydroxylamine is more active than NH4+
-
?
ATP + L-Asp + hydroxylamine
AMP + diphosphate + Asn + beta-aspartyl hydroxamate
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hydroxylamine is more active than NH4+
-
?
ATP + L-Asp + NH4+
AMP + diphosphate + Asn
-
-
-
-
?
ATP + L-Asp + NH4+
AMP + diphosphate + Asn
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no activity with ADP, CTP, UTP, or GTP
-
-
?
ATP + L-Asp + NH4+
AMP + diphosphate + Asn
-
-
-
-
?
ATP + L-Asp + NH4+
AMP + diphosphate + Asn
-
no activity with GTP
-
-
?
ATP + L-Asp + NH4+
AMP + diphosphate + Asn
-
-
-
-
?
ATP + L-Asp + NH4+
AMP + diphosphate + Asn
-
-
-
-
?
ATP + L-Asp + NH4+
AMP + diphosphate + Asn
-
-
-
-
?
ATP + L-Asp + NH4+
AMP + diphosphate + Asn
-
-
-
-
?
ATP + L-aspartate + glutamine
?
-
-
-
-
?
ATP + L-aspartate + glutamine
?
-
-
-
-
?
ATP + L-aspartate + NH3
AMP + diphosphate + L-asparagine
-
-
-
?
ATP + L-aspartate + NH3
AMP + diphosphate + L-asparagine
-
-
-
?
ATP + L-aspartate + NH3
AMP + diphosphate + L-asparagine
-
-
-
-
?
ATP + L-aspartate + NH3
AMP + diphosphate + L-asparagine
-
-
-
-
?
ATP + L-aspartate + NH3
AMP + diphosphate + L-asparagine
-
-
-
-
?
additional information
?
-
-
L-Asp-dependent ATP-diphosphate exchange in the absence of added NH4+
-
-
?
additional information
?
-
-
no activity with glutamine
-
-
?
additional information
?
-
-
importance of ASNS protein in the cellular mechanisms that confer drug resistance upon the leukemic cells
-
-
?
additional information
?
-
-
no activity with glutamine
-
-
?
additional information
?
-
-
no activity with glutamine
-
-
?
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crystal structure of native AsnA and complexed with L-asparagine and AMP at 2.5 A, 2.2 A and 2.2 A resolution, respectively
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crystallized in two different conditions using the hanging-drop vapour-diffusion method. Crystals belonging to space group C2 with unit-cell parameters a = 103.6, b = 43.3, c = 121.5 A, beta = 112.6° and one dimer per asymmetric unit are obtained in the presence of 2-propanol and PEG 4000 at pH 5.6. Another crystal form is obtained in the presence of dioxan and belongs to the monoclinic space group P2(1), with unit-cell parameters a = 96.8, b = 103.9, c = 98.4 A, beta = 107.5° and two dimers per asymmetric unit. Two different native diffraction data sets are collected to 2.3 A and 3.0 A resolution using synchrotron radiation and cryocooling for crystals belonging to space groups C2 and P2(1), respectively
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sitting-drop vapor diffusion. The fold of this protein is similar to that of bacterial asparagine synthetase A and resembles the catalytic cores of aspartyl-tRNA synthetase and asparaginyl-tRNA synthetase
purified recombinant His6-tagged apoenzyme, hanging drop vapor diffusion method, mixing of 0.001 ml of 73 mg/ml protein in 50 mM Tris-Cl, 200 mM NaCl, and 10 mM 2-mercaptoethanol, pH 7.5, with 0.001 ml of reservoir solution containing 0% w/v PEG 20000, 20% v/v PEG monomethyl ether 550, 0.03 M NPS (sodium nitrate, disodium hydrogen phosphate, ammonium sulfate), and 0.1 M MOPS/HEPES-Na, pH 7.5, 20°C, X-ray diffraction structure determination and analysis at 2.2 A resolution, modeling
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bacterial gene is placed under control of light-dependent promoters, and introduced by transformation into Lotus corniculatus plants. The asnA-expressing plants are characterized by premature flowering and reduced growth. Transformation with asnA also induces a significant reduction of photosynthesis when measured under saturated light and ambient CO2 conditions
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expressed in Escherichia coli BL21 cells
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expression analysis in HEK-293 cells in response to ATF4 or CHOP
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expression of Escherichia coli asnA gene in Brassica napus could be of advantage at high N supply, but not at limiting N ´fertilizer supply
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gene Tb927.6.1880, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21
quantitative enzyme expression analysis in Hep-G2 cells in absence or presence of UPR blockers and activators, transcription factor recruitment to the ASNS promoter during the UPR, transcriptional activation of UPR target genes is mediated by three signaling cascades PERK/eIF2alpha/ATF4, ATF6, and IRE1/XBP1, UPR activation does not trigger increased recruitment of Mediator subunits to the ASNS promoter, the IRE1/XBP1 and ATF6 branches of the UPR do not participate in the induction of ASNS transcription, overview
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the enzyme is obtained by means of a plasmid, pUNAd37, a derivative of pUC18 in Escherichia coli. The plasmid is constructed by optimizing a DNA sequence between the promoter and the ribosome binding region
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Bogdahn, M.; Kleiner, D.
N2 fixation and NH4+ assimilation in the thermophilic anaerobes Clostridium thermosaccharolyticum and Clostridium thermoautotrophicum
Arch. Microbiol.
144
102-104
1986
Moorella thermoautotrophica, Moorella thermoautotrophica JW 79/3, Thermoanaerobacterium thermosaccharolyticum
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Asparagine synthesis
The Enzymes, 3rd Ed. (Boyer, P. D. , ed. )
10
561-580
1974
Escherichia coli, Lactiplantibacillus plantarum, Streptococcus equinus
-
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Hinchman, S.K.; Schuster, S.M.
Overproduction, preparation of monoclonal antibodies and purification of E. coli asparagine synthetase A
Protein Eng.
5
279-283
1992
Escherichia coli
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Nakatsu, T.; Kato, H.; Oda, J.
Crystallization and preliminary crystallographic study of asparagine synthetase from Escherichia coli
Acta Crystallogr. Sect. D
52
604-606
1996
Escherichia coli
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Reitzer, L.J.; Magasanik, B.
Asparagine synthetases of Klebsiella aerogenes: properties and regulation of synthesis
J. Bacteriol.
151
1299-1313
1982
Klebsiella aerogenes
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Sugiyama, A.; Kato, H.; Nishioka, T.; Oda, J.
Overexpression and purification of asparagine synthetase from Escherichia coli
Biosci. Biotechnol. Biochem.
56
376-379
1992
Escherichia coli
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Boehlein, S.K.; Nakatsu, T.; Hiratake, J.; Thirumoorty, R.; Stewart, J.D.; Richards, N.G.J.; Schuster, S.M.
Characterization of inhibitors acting at the synthetase site of Escherichia coli asparagine synthetase B
Biochemistry
40
11168-11175
2001
Escherichia coli
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Nakatsu, T.; Kato, H.; Oda, J.
Crystal structure of asparagine synthetase reveals a close evolutionary relationship to class II aminoacyl-tRNA synthetase
Nat. Struct. Biol.
5
15-19
1998
Escherichia coli
brenda
Charron, C.; Roy, H.; Blaise, M.; Giege, R.; Kern, D.
Crystallization and preliminary X-ray diffraction data of an archaeal asparagine synthetase related to asparaginyl-tRNA synthetase
Acta Crystallogr. Sect. D
60
767-769
2004
Pyrococcus abyssi
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Seiffert, B.; Zhou, Z.; Wallbraun, M.; Lohaus, G.; Moellers, C.
Expression of a bacterial asparagine synthetase gene in oilseed rape (Brassica napus) and its effect on traits related to nitrogen efficiency
Physiol. Plant.
121
656-665
2004
Escherichia coli
-
brenda
Bellucci, M.; Ederli, L.; De Marchis, F.; Pasqualini, S.; Arcioni, S.
Transformation of Lotus corniculatus plants with Escherichia coli asparagine synthetase A: Effect on nitrogen assimilation and plant development
Plant Cell Tissue Organ Cult.
78
139-150
2004
Escherichia coli
-
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Richards, N.G.; Kilberg, M.S.
Asparagine synthetase chemotherapy
Annu. Rev. Biochem.
75
629-654
2006
Escherichia coli
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Gjymishka, A.; Su, N.; Kilberg, M.S.
Transcriptional induction of the human asparagine synthetase gene during the unfolded protein response does not require the ATF6 and IRE1/XBP1 arms of the pathway
Biochem. J.
417
695-703
2008
Homo sapiens
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Su, N.; Kilberg, M.S.
C/EBP homology protein (CHOP) interacts with activating transcription factor 4 (ATF4) and negatively regulates the stress-dependent induction of the asparagine synthetase gene
J. Biol. Chem.
283
35106-35117
2008
Homo sapiens
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Mass spectrometric quantification of asparagine synthetase in circulating leukemia cells from acute lymphoblastic leukemia patients
J. Proteomics
71
61-70
2008
Homo sapiens
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Lorenzi, P.L.; Llamas, J.; Gunsior, M.; Ozbun, L.; Reinhold, W.C.; Varma, S.; Ji, H.; Kim, H.; Hutchinson, A.A.; Kohn, E.C.; Goldsmith, P.K.; Birrer, M.J.; Weinstein, J.N.
Asparagine synthetase is a predictive biomarker of L-asparaginase activity in ovarian cancer cell lines
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7
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2008
Homo sapiens
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Blaise, M.; Frechin, M.; Olieric, V.; Charron, C.; Sauter, C.; Lorber, B.; Roy, H.; Kern, D.
Crystal structure of the archaeal asparagine synthetase: interrelation with aspartyl-tRNA and asparaginyl-tRNA synthetases
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412
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2011
Pyrococcus abyssi (Q9V228), Pyrococcus abyssi, Pyrococcus abyssi GE5 / CNCM I-1302 / DSM 25543 (Q9V228)
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Identification and functional characterization of a novel bacterial type asparagine synthetase A a tRNA synthetase paralog from Leishmania donovani
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289
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