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Information on EC 7.4.2.7 - ABC-type alpha-factor-pheromone transporter
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7.4.2.7 ABC-type alpha-factor-pheromone transporterIUBMB Comments
An ATP-binding cassette (ABC) type transporter, characterized by the presence of two similar ATP-binding domains/proteins and two integral membrane domains/proteins. Does not undergo phosphorylation during the transport process. A yeast enzyme that exports the alpha-factor sex pheromone.
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The enzyme appears in viruses and cellular organisms
Reaction Schemes
+
+
alpha-factor[side 1]
=
+
+
alpha-factor[side 2]
Synonyms
adenosine triphosphate-binding cassette transporter, ste6p, ste6 gene product, 
more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
ABC transporter Ste6
alpha-factor-transporting ATPase
EC 3.6.3.48
-
-
formerly
-
Ste6
Ste6p
alpha-factor-transporting ATPase
-
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ATP + H2O + alpha-factor[side 1] = ADP + phosphate + alpha-factor[side 2]
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of phosphate bond
-
-
-
-
transmembrane transport
transmembrane transport
-
-
-
-
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SYSTEMATIC NAME
IUBMB Comments
ATP phosphohydrolase (ABC-type, alpha-factor-exporting)
An ATP-binding cassette (ABC) type transporter, characterized by the presence of two similar ATP-binding domains/proteins and two integral membrane domains/proteins. Does not undergo phosphorylation during the transport process. A yeast enzyme that exports the alpha-factor sex pheromone.
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CAS REGISTRY NUMBER
COMMENTARY
9000-83-3
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
additional information
?
-
-
Ste6p plays an essential role in the export of farnesyl-modified a-type mating factors, close relationship between the ability of this class of transporters to export drugs and lipid-modified signaling molecules
-
-
?
ATP + H2O + alpha-factor/in
ADP + phosphate + alpha-factor/out
-
-
-
?
ATP + H2O + alpha-factor/in
ADP + phosphate + alpha-factor/out
-
-
-
?
ATP + H2O + alpha-factor/in
ADP + phosphate + alpha-factor/out
-
-
-
?
ATP + H2O + alpha-factor/in
ADP + phosphate + alpha-factor/out
-
-
-
?
ATP + H2O + alpha-factor/in
ADP + phosphate + alpha-factor/out
-
-
-
?
ATP + H2O + alpha-factor/in
ADP + phosphate + alpha-factor/out
-
-
-
?
ATP + H2O + alpha-factor/in
ADP + phosphate + alpha-factor/out
-
-
-
?
ATP + H2O + alpha-factor/in
ADP + phosphate + alpha-factor/out
-
-
-
?
ATP + H2O + alpha-factor/in
ADP + phosphate + alpha-factor/out
-
-
-
-
?
ATP + H2O + alpha-factor/in
ADP + phosphate + alpha-factor/out
-
-
-
?
ATP + H2O + alpha-factor/in
ADP + phosphate + alpha-factor/out
-
the STE6 gene product is required for secretion of the lipopeptide mating pheromone alpha-factor by Saccharomyces cerevisiae MATa cells
-
?
ATP + H2O + alpha-factor/in
ADP + phosphate + alpha-factor/out
-
enzyme is responsible for transporting the alpha-factor mating pheromone out of the cell, thus performing the final step in alpha-factor biogenesis
-
?
ATP + H2O + alpha-factor/in
ADP + phosphate + alpha-factor/out
-
enzyme is responsible for transporting the alpha-factor mating pheromone out of the cell, thus performing the final step in alpha-factor biogenesis
-
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
additional information
?
-
-
Ste6p plays an essential role in the export of farnesyl-modified a-type mating factors, close relationship between the ability of this class of transporters to export drugs and lipid-modified signaling molecules
-
-
?
ATP + H2O + alpha-factor/in
ADP + phosphate + alpha-factor/out
-
-
-
?
ATP + H2O + alpha-factor/in
ADP + phosphate + alpha-factor/out
-
-
-
?
ATP + H2O + alpha-factor/in
ADP + phosphate + alpha-factor/out
-
-
-
?
ATP + H2O + alpha-factor/in
ADP + phosphate + alpha-factor/out
-
-
-
-
?
ATP + H2O + alpha-factor/in
ADP + phosphate + alpha-factor/out
-
the STE6 gene product is required for secretion of the lipopeptide mating pheromone alpha-factor by Saccharomyces cerevisiae MATa cells
-
?
ATP + H2O + alpha-factor/in
ADP + phosphate + alpha-factor/out
-
enzyme is responsible for transporting the alpha-factor mating pheromone out of the cell, thus performing the final step in alpha-factor biogenesis
-
?
ATP + H2O + alpha-factor/in
ADP + phosphate + alpha-factor/out
-
enzyme is responsible for transporting the alpha-factor mating pheromone out of the cell, thus performing the final step in alpha-factor biogenesis
-
?
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DISEASE
TITLE OF PUBLICATION
LINK TO PUBMED
Adenocarcinoma of Lung
MiR-200b-3p Functions as an Oncogene by Targeting ABCA1 in Lung Adenocarcinoma.
Adrenoleukodystrophy
Hematopoietic stem cell gene therapy with a lentiviral vector in X-linked adrenoleukodystrophy.
Adrenoleukodystrophy
Lentiviral hematopoietic cell gene therapy for x-linked adrenoleukodystrophy.
Aortic Aneurysm, Abdominal
miR-33-5p knockdown attenuates abdominal aortic aneurysm progression via promoting target adenosine triphosphate-binding cassette transporter A1 expression and activating the PI3K/Akt signaling pathway.
Arteriosclerosis
Relationship between ABCA1 gene polymorphism and lacunar infarction combined with arteriosclerosis in patients.
Atherosclerosis
Seeking novel targets for improving in vivo macrophage-specific reverse cholesterol transport: translating basic science into new therapies for the prevention and treatment of atherosclerosis.
Breast Neoplasms
Atypical multidrug resistance: breast cancer resistance protein messenger RNA expression in mitoxantrone-selected cell lines.
Breast Neoplasms
Estrone and 17beta-estradiol reverse breast cancer resistance protein-mediated multidrug resistance.
Carcinoma, Hepatocellular
Adenosine triphosphate-binding cassette transporter genes up-regulation in untreated hepatocellular carcinoma is mediated by cellular microRNAs.
Cardiovascular Diseases
Circulating Nef induces dyslipidemia in simian immunodeficiency virus-infected macaques by suppressing cholesterol efflux.
Carotid Artery Diseases
The influence of two variants in the adenosine triphosphate-binding cassette transporter 1 gene on plasma lipids and carotid atherosclerosis.
Cholestasis
Alteration of the expression of adenosine triphosphate-binding cassette transporters associated with bile acid and cholesterol transport in the rat liver and intestine during cholestasis.
Cholestasis, Intrahepatic
Technical pitfalls and improvements for high-speed screening and QSAR analysis to predict inhibitors of the human bile salt export pump (ABCB11/BSEP).
Cholesteatoma
Are human ATP-binding cassette transporter C11 and earwax associated with the incidence of cholesteatoma?
Coronary Artery Disease
Associations between common polymorphisms of adenosine triphosphate-binding cassette transporter A1 and coronary artery disease in a Tunisian population.
Coronary Disease
Evaluation of Adenosine Triphosphate-Binding Cassette Transporter A1 (ABCA1) R219K and C-Reactive Protein Gene (CRP) +1059G/C Gene Polymorphisms in Susceptibility to Coronary Heart Disease.
Coronary Disease
Relationships between common polymorphisms of adenosine triphosphate-binding cassette transporter A1 and high-density lipoprotein cholesterol and coronary heart disease in a population with type 2 diabetes mellitus.
Cystic Fibrosis
Bacterial periplasmic permeases belong to a family of transport proteins operating from Escherichia coli to human: Traffic ATPases.
Cystic Fibrosis
Structural model of ATP-binding proteins associated with cystic fibrosis, multidrug resistance and bacterial transport.
Demyelinating Diseases
Hematopoietic stem cell gene therapy with a lentiviral vector in X-linked adrenoleukodystrophy.
Demyelinating Diseases
Lentiviral hematopoietic cell gene therapy for x-linked adrenoleukodystrophy.
Diabetes Mellitus
Determinants of leukocyte adenosine triphosphate-binding cassette transporter G1 gene expression in type 2 diabetes mellitus.
Diabetes Mellitus
High density lipoproteins in the intersection of diabetes mellitus, inflammation and cardiovascular disease.
Diabetes Mellitus
Relationships between common polymorphisms of adenosine triphosphate-binding cassette transporter A1 and high-density lipoprotein cholesterol and coronary heart disease in a population with type 2 diabetes mellitus.
Diabetes Mellitus, Type 2
Determinants of leukocyte adenosine triphosphate-binding cassette transporter G1 gene expression in type 2 diabetes mellitus.
Diabetes Mellitus, Type 2
Relationships between common polymorphisms of adenosine triphosphate-binding cassette transporter A1 and high-density lipoprotein cholesterol and coronary heart disease in a population with type 2 diabetes mellitus.
Dyslipidemias
Circulating Nef induces dyslipidemia in simian immunodeficiency virus-infected macaques by suppressing cholesterol efflux.
Endometriosis
Adenosine triphosphate-binding cassette transporter G2 expression in endometriosis and in endometrium from patients with and without endometriosis.
Gallstones
Sterol transporter adenosine triphosphate-binding cassette transporter G8, gallstones, and biliary cancer in 62,000 individuals from the general population.
Hyperlipidemias
Associations of the ABCA1 and LPL Gene Polymorphisms With Lipid Levels in a Hyperlipidemic Population.
Hyperparathyroidism, Primary
Hypermethylation of adenosine triphosphate-binding cassette transporter genes in primary hyperparathyroidism and its effect on sestamibi imaging.
Infections
Circulating Nef induces dyslipidemia in simian immunodeficiency virus-infected macaques by suppressing cholesterol efflux.
Lung Diseases, Interstitial
A New ABCA3 Gene Mutation c.3445G>A (p.Asp1149Asn) as a Causative Agent of Newborn Lethal Respiratory Distress Syndrome.
Lung Diseases, Interstitial
Different course of lung disease in two siblings with novel ABCA3 mutations.
Lung Diseases, Interstitial
Pulmonary Nodules in a Newborn With ATP-Binding Cassette Transporter A3 (ABCA3)Mutations.
Neoplasm Metastasis
MiR-200b-3p Functions as an Oncogene by Targeting ABCA1 in Lung Adenocarcinoma.
Neoplasms
A comparative small-animal PET evaluation of [11C]tariquidar, [11C]elacridar and (R)-[11C]verapamil for detection of P-glycoprotein-expressing murine breast cancer.
Neoplasms
Danhong injection inhibits the development of atherosclerosis in both Apoe?/? and Ldlr?/? mice.
Neoplasms
Interaction of HM30181 with P-glycoprotein at the murine blood-brain barrier assessed with positron emission tomography.
Neoplasms
Mechanisms of chemotherapeutic drug resistance in cancer therapy--a quick review.
Neoplasms
P-glycoprotein expression and regulation. Age-related changes and potential effects on drug therapy.
Neoplasms
Sterol transporter adenosine triphosphate-binding cassette transporter G8, gallstones, and biliary cancer in 62,000 individuals from the general population.
Neoplasms
Structural model of ATP-binding proteins associated with cystic fibrosis, multidrug resistance and bacterial transport.
Neoplasms
The bovine ATP-binding cassette transporter ABCG2 Tyr581Ser single-nucleotide polymorphism increases milk secretion of the fluoroquinolone danofloxacin.
Neuromyelitis Optica
Gut microbiome analysis in neuromyelitis optica reveals overabundance of Clostridium perfringens.
Obesity
Ablation of the cholesterol transporter adenosine triphosphate-binding cassette transporter G1 reduces adipose cell size and protects against diet-induced obesity.
Pseudoxanthoma Elasticum
Genetic deletion of Abcc6 disturbs cholesterol homeostasis in mice.
Reperfusion Injury
Repression of adenosine triphosphate-binding cassette transporter ABCG2 by estrogen increases intracellular glutathione in brain endothelial cells following ischemic reperfusion injury.
Respiratory Distress Syndrome, Newborn
A New ABCA3 Gene Mutation c.3445G>A (p.Asp1149Asn) as a Causative Agent of Newborn Lethal Respiratory Distress Syndrome.
Respiratory Insufficiency
Respiratory failure in a term newborn due to compound heterozygous ABCA3 mutation: the case report of another lethal variant.
Stomach Neoplasms
Significantly increased expression of OCT4 and ABCG2 in spheroid body-forming cells of the human gastric cancer MKN-45 cell line.
Stroke
miR-145 Regulates Diabetes-Bone Marrow Stromal Cell-Induced Neurorestorative Effects in Diabetes Stroke Rats.
Stroke, Lacunar
Relationship between ABCA1 gene polymorphism and lacunar infarction combined with arteriosclerosis in patients.
Tangier Disease
Clinical, Electrodiagnostic, and Genetic Features of Tangier Disease in an Adolescent Girl with Presentation of Peripheral Neuropathy.
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
additional information
-
fusion of ubiquitin to mutant enzyme Ste6p-deltaA dramatically increased its rate of turnover
-
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
MATa his3-DELTA200 leu2-3,112 lys2-801 DELTAste6::LEU2 trp1-DELTA63 ura3-52
-
-
brenda
-
-
-
brenda
MATa his3-DELTA200 leu2-3,112 lys2-801 DELTAste6::LEU2 trp1-DELTA63 ura3-52
-
-
brenda
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
-
enzyme variant with a deletion in the linker region
brenda
-
integral membrane protein, at least a portion of the enzyme is exposed at the exocellular surface
brenda
-
newly synthesized enzyme first travels to the cell surface via the secretory pathway, endoplasmic reticulum, Golgi, secretory vesicles, resides in the plasma membrane transiently and subsequently undergoes endocytosis and degradation in the vacuole
brenda
-
ubiquitinated Ste6 is associated with. Ubiquitination of Ste6 or of a trafficing factor is required for Ste6 sorting into the multivesivular bodies pathway. Ste6 recycles between an internal compartment and the plasma membrane
brenda
-
accumulates in the plasma membrane in a ubiquintinated form in endocytosis mutants
brenda
-
at the nonpermissive temperature Ste6p-GFP is found exclusively at the plasma membrane
brenda
-
enters the secretory pathway and is transported to the vacuole by vesicular carriers
brenda
-
ubiquitination is required for the uptake of Ste6 into the lumen of the vacuole, uptake of Ste6 into the yeast vacuole is blocked in the doa4 mutant
brenda
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UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). HST6_CANAW
Candida albicans (strain WO-1)
1323
11
148502
Swiss-Prot
Mitochondrion (Reliability: 4)
STE6_YEAST
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
1290
10
144766
Swiss-Prot
Mitochondrion (Reliability: 4)
A0A125YSH6_TOXGM
Toxoplasma gondii (strain ATCC 50611 / Me49)
1345
7
144529
TrEMBL
other Location (Reliability: 2)
A0A086M8T1_TOXGO
127
0
13897
TrEMBL
other Location (Reliability: 1)
A0A086QRK6_TOXGO
283
4
30141
TrEMBL
Secretory Pathway (Reliability: 5)
A0A139XKL6_TOXGO
650
4
70715
TrEMBL
other Location (Reliability: 1)
A0A2G8Y2G3_TOXGO
1019
4
109764
TrEMBL
other Location (Reliability: 4)
A0A086K7S3_TOXGO
326
0
34904
TrEMBL
Secretory Pathway (Reliability: 4)
A0A086K7T1_TOXGO
382
6
40245
TrEMBL
other Location (Reliability: 2)
A0A086KG67_TOXGO
119
0
13434
TrEMBL
other Location (Reliability: 4)
A0A139XS27_TOXGO
356
6
37468
TrEMBL
other Location (Reliability: 2)
A0A086KG71_TOXGO
1153
4
124569
TrEMBL
other Location (Reliability: 4)
A0A2G8YE43_TOXGO
289
0
31285
TrEMBL
Secretory Pathway (Reliability: 5)
A0A086LQ59_TOXGO
421
5
46866
TrEMBL
Mitochondrion (Reliability: 2)
A0A086K7R4_TOXGO
288
2
31571
TrEMBL
Secretory Pathway (Reliability: 4)
A0A086PU42_TOXGO
1846
12
200551
TrEMBL
Mitochondrion (Reliability: 2)
A0A086QRK1_TOXGO
108
1
11304
TrEMBL
other Location (Reliability: 1)
A0A086JKF6_TOXGO
1722
12
187563
TrEMBL
Mitochondrion (Reliability: 2)
A0A086K6J3_TOXGO
716
6
75966
TrEMBL
other Location (Reliability: 2)
A0A139XKM1_TOXGO
597
4
64472
TrEMBL
Secretory Pathway (Reliability: 2)
A0A086QDS0_TOXGO
291
0
31473
TrEMBL
Secretory Pathway (Reliability: 5)
A0A086LQ63_TOXGO
747
4
81583
TrEMBL
Mitochondrion (Reliability: 2)
A0A086QRK9_TOXGO
289
0
31255
TrEMBL
Secretory Pathway (Reliability: 5)
A0A139XKM0_TOXGO
421
5
46820
TrEMBL
Mitochondrion (Reliability: 2)
A0A086M8T2_TOXGO
301
0
32647
TrEMBL
Secretory Pathway (Reliability: 5)
A0A3R8A4W1_TOXGO
1395
4
151849
TrEMBL
Mitochondrion (Reliability: 2)
A0A086KHU5_TOXGO
122
0
13652
TrEMBL
other Location (Reliability: 4)
A0A086QEZ3_TOXGO
1841
12
200184
TrEMBL
Mitochondrion (Reliability: 3)
A0A3R7YR36_TOXGO
401
6
42477
TrEMBL
other Location (Reliability: 2)
A0A086QDS5_TOXGO
1033
7
110402
TrEMBL
other Location (Reliability: 2)
A0A086K7Q3_TOXGO
299
0
32446
TrEMBL
other Location (Reliability: 5)
S8FD11_TOXGM
Toxoplasma gondii (strain ATCC 50611 / Me49)
1942
12
211568
TrEMBL
Mitochondrion (Reliability: 3)
A0A2G8YDB3_TOXGO
108
1
11274
TrEMBL
other Location (Reliability: 1)
V4ZBS8_TOXGV
Toxoplasma gondii (strain ATCC 50861 / VEG)
1951
12
212215
TrEMBL
Mitochondrion (Reliability: 2)
A0A086K6H8_TOXGO
329
0
35864
TrEMBL
Secretory Pathway (Reliability: 5)
A0A086KHS7_TOXGO
747
4
81485
TrEMBL
Mitochondrion (Reliability: 2)
M5C887_THACB
Thanatephorus cucumeris (strain AG1-IB / isolate 7/3/14)
1011
5
110019
TrEMBL
other Location (Reliability: 2)
A0A086KG77_TOXGO
358
3
39936
TrEMBL
other Location (Reliability: 4)
A0A2G8YDB5_TOXGO
303
0
32429
TrEMBL
other Location (Reliability: 1)
A0A2G8YDC4_TOXGO
356
1
38791
TrEMBL
Mitochondrion (Reliability: 3)
A0A086LQ62_TOXGO
576
3
61767
TrEMBL
Secretory Pathway (Reliability: 1)
A0A086QRK8_TOXGO
356
1
38791
TrEMBL
Mitochondrion (Reliability: 3)
A0A086JPU0_TOXGO
1345
7
144529
TrEMBL
other Location (Reliability: 2)
A0A0F7V2P0_TOXGV
Toxoplasma gondii (strain ATCC 50861 / VEG)
1345
7
144499
TrEMBL
other Location (Reliability: 2)
A0A086QRK7_TOXGO
303
0
32429
TrEMBL
other Location (Reliability: 1)
S7UJZ9_TOXGG
Toxoplasma gondii (strain ATCC 50853 / GT1)
1942
12
211637
TrEMBL
Mitochondrion (Reliability: 3)
A0A2G8YDB8_TOXGO
272
4
28945
TrEMBL
Secretory Pathway (Reliability: 5)
A0A086JKG1_TOXGO
122
0
13652
TrEMBL
other Location (Reliability: 4)
A0A086KHS0_TOXGO
997
8
108606
TrEMBL
Mitochondrion (Reliability: 2)
A0A3R7YK28_TOXGO
285
0
30921
TrEMBL
other Location (Reliability: 2)
A0A2G8Y2H2_TOXGO
825
8
90445
TrEMBL
Mitochondrion (Reliability: 3)
A0A2G8Y2F0_TOXGO
119
0
13434
TrEMBL
other Location (Reliability: 4)
A0A086M8S8_TOXGO
895
8
95415
TrEMBL
other Location (Reliability: 2)
A0A086LQ68_TOXGO
122
0
13652
TrEMBL
other Location (Reliability: 4)
A0A125YUY7_TOXGG
Toxoplasma gondii (strain ATCC 50853 / GT1)
1345
7
144499
TrEMBL
other Location (Reliability: 2)
A0A0F8CZY2_CERFI
947
5
102988
TrEMBL
other Location (Reliability: 4)
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
-
a 1323-amino acid protein with an intramolecular duplicated structure, each repeated half containing six potential hydrophobic transmembrane segments and a hydrophilic domain
additional information
-
the enzyme contains two homologous halves, each with six membrane spanning segments and a predicted ATP nucleotide binding domain. Both halves of STE6 are critical for function
additional information
-
multispanning membrane protein with 12 transmembrane spans and two cytosolic ATP binding domains
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POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
phosphoprotein
additional information
-
accumulates in the plasma membrane in a ubiquintinated form in endocytosis mutants
phosphoprotein
-
Ste6 is phosphorylated in vivo, this phosphorylation is dependent on the presence of an acidic stretch (A-box) in the linker region shown to be important for ubiquitination and fast turnover of Ste6
phosphoprotein
-
Ste6 is phosphorylated in vivo, this phosphorylation is dependent on the presence of an acidic stretch (A-box) in the linker region shown to be important for ubiquitination and fast turnover of Ste6
-
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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
A517P
mutant enzyme shows 0.01% of the mating efficiency of the wild-type enzyme
A517T
-
mates with wild-type efficiency at 30°C. The organism shows 2-3fold decrease in mating at 37°C
A532H
-
mates with wild-type efficiency at 30°C. The organism shows 2-3fold decrease in mating at 37°C
DELTA-Abox
-
concurrent loss of phosphorylation and ubiquitination in the A-box mutant
E439K
mutant enzyme shows 0.01% of the mating efficiency of the wild-type enzyme
E531K/V533I
mutant enzyme shows 0.75% of the mating efficiency of the wild-type enzyme
F630L
-
mutant shows polar cell surface staining, indistinguishable from wild-type Ste6
F656L
-
mutant shows polar cell surface staining, indistinguishable from wild-type Ste6
G320D
mutant enzyme shows 5.5% of the mating efficiency of the wild-type enzyme
G320V
mutant enzyme shows 6% of the mating efficiency of the wild-type enzyme
G38D
mutant enzyme is completly defective for mating, mutant enzyme does not accumulate in plasma membrane fraction
G38N
mutant enzyme shows 0.06-0.01% of the mating efficiency of the wild-type enzyme
G397N
mutant enzyme shows 1.7% of the mating efficiency of the wild-type enzyme
G509D
-
significant reduction in alpha-factor transport. The organism shows 56fold decrease in mating relative to wild-type control
I521N
-
mates with wild-type efficiency at 30°C. The organism shows 2-3fold decrease in mating at 37°C
P525L
mutant enzyme shows 7% of the mating efficiency of the wild-type enzyme at 25°C, 0.06% at 30°C and is defective for mating at 37°C
R518T
-
mates with wild-type efficiency at 30°C. The organism shows 11fold reduction in mating at 37°C
R833K/R941H
mutant enzyme shows 1.9% of the mating efficiency of the wild-type enzyme
S507I
-
less severe reduction in alpha-factor transport. The organism shows a mating defect at 30°C
S507N
S507N/G508S/G509R
mutant enzyme is completely defective for mating
S507R
-
significant reduction in alpha-factor transport. The organism shows a mating defect at 30°C
S623L
-
3fold increased stability compared to wild-type enzyme, phosphorylation is reduced
S632F/Q633STOP
mutant enzyme shows 1.8% of the mating efficiency of the wild-type enzyme
T613A
-
2fold increased stability compared to wild-type enzyme, phosphorylation is not affected
T613A/S623L
-
5fold increased stability compared to wild-type enzyme
Y648L
-
mutant shows polar cell surface staining, indistinguishable from wild-type Ste6
Y657L
-
mutant shows polar cell surface staining, indistinguishable from wild-type Ste6
Y661L
-
mutant shows polar cell surface staining, indistinguishable from wild-type Ste6
Y713L
-
mutant shows polar cell surface staining, indistinguishable from wild-type Ste6
DELTA-Abox
-
concurrent loss of phosphorylation and ubiquitination in the A-box mutant
-
S623L
-
3fold increased stability compared to wild-type enzyme, phosphorylation is reduced
-
T613A
-
2fold increased stability compared to wild-type enzyme, phosphorylation is not affected
-
T613A/S623L
-
5fold increased stability compared to wild-type enzyme
-
S507N
-
significant reduction in alpha-factor transport. The organism shows 90fold decrease in mating relative to the wild-type control
S507N
mutant enzyme shows 0.5-0.85% of the mating efficiency of the wild-type enzyme
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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
the very unstable protein is stabilized approximately 3fold in a ubc4 ubc5 mutant, strongest stabilization in the vacuolar pep4 mutant
-
two independent degradative systems, the vacuolar Pep4p-dependent proteases and the cytosolic proteasome are both involved, either directly or indirectly, in the metabolic degradation of the alpha-factor-transporting ATPase
-
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Berkower, C.; Loayza, D.; Michaelis, S.
Metabolic instability and constitutive endocytosis of STE6, the alpha-factor transporter of Saccharomyces cerevisiae
Mol. Biol. Cell
5
1185-1198
1994
Saccharomyces cerevisiae
brenda
Berkower, C.; Michaelis, S.
Mutational analysis of the yeast alpha-factor transporter STE6, a member of the ATP binding cassette (ABC) protein superfamily
EMBO J.
10
3777-3785
1991
Saccharomyces cerevisiae
brenda
Browne, B.L.; McClendon, V.; Bedwell, D.M.
Mutations within the first LSGGQ motif of Ste6p cause defects in alpha-factor transport and mating in Saccharomyces cerevisiae
J. Bacteriol.
178
1712-1719
1996
Saccharomyces cerevisiae
brenda
Raymond, M.; Dignard, D.; Alarco, A.M.; Mainville, N.; Magee, B.B.; Thomas, D.Y.
A Ste6p/P-glycoprotein homologue from the asexual yeast Candida albicans transports the alpha-factor mating pheromone in Saccharomyces cerevisiae
Mol. Microbiol.
27
587-598
1998
Saccharomyces cerevisiae, Candida albicans
brenda
Kuchler, K.; Dohlman, H.G.; Thorner, J.
The alpha-factor transporter (STE6 gene product) and cell polarity in the yeast Saccharomyces cerevisiae
J. Cell Biol.
120
1203-1215
1993
Saccharomyces cerevisiae
brenda
Loayza, D.; Michaelis, S.
Role for the ubiquitin-proteasome system in the vacuolar degradation of Ste6p, the alpha-factor transporter in Saccharomyces cerevisiae
Mol. Cell. Biol.
18
779-789
1998
Saccharomyces cerevisiae
brenda
Michaelis, S.
STE6, the yeast alpha-factor transporter
Semin. Cell Biol.
4
17-27
1993
Saccharomyces cerevisiae
brenda
Klling, R.; Hollenberg, C.P.
The ABC-transporter Ste6 accumulates in the plasma membrane in a ubiquintinated form in endocytosis mutants
EMBO J.
13
3261-3271
1994
Saccharomyces cerevisiae
brenda
Klling, R.
Mutations affecting phosphorylation, ubiquitination and turnover of the ABC-transporter Ste6
FEBS Lett.
531
548-552
2002
Saccharomyces cerevisiae, Saccharomyces cerevisiae RKY959
brenda
Losko, S.; Kopp, F.; Kranz, A.; Kolling, R.
Uptake of the ATP-binding cassette (ABC) transporter Ste6 into the yeast vacuole is blocked in the doa4 mutant
Mol. Biol. Cell
12
1047-1059
2001
Saccharomyces cerevisiae
brenda
Proff, C.; Klling, R.
Functional asymmetry of the two nucleotide binding domains in the ABC transporter Ste6
Mol. Gen. Genet.
264
883-893
2001
Saccharomyces cerevisiae (P12866), Saccharomyces cerevisiae
brenda
Krsmanovic, T.; Pawelec, A.; Sydor, T.; Koelling, R.
Control of Ste6 recycling by ubiquitination in the early endocytic pathway in yeast
Mol. Biol. Cell
16
2809-2821
2005
Saccharomyces cerevisiae
brenda
Kelm, K.B.; Huyer, G.; Huang, J.C.; Michaelis, S.
The internalization of yeast Ste6p follows an ordered series of events involving phosphorylation, ubiquitination, recognition and endocytosis
Traffic
5
165-180
2004
Saccharomyces cerevisiae
brenda
Huyer, G.; Kistler, A.; Nouvet, F.J.; George, C.M.; Boyle, M.L.; Michaelis, S.
Saccharomyces cerevisiae alpha-factor mutants reveal residues critical for processing, activity, and export
Eukaryot. Cell
5
1560-1570
2006
Saccharomyces cerevisiae
brenda
Ricardo, S.; Lehmann, R.
An ABC transporter controls export of a Drosophila germ cell attractant
Science
323
943-946
2009
Drosophila melanogaster
brenda
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EXTERNAL LINKS (specific for EC-Number 7.4.2.7)
Transporter Classification Database (TCDB): 3.A.1.206.1
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Release 2023.1 (January 2023)
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