Any feedback?
Please rate this page
(ligand.php)
(0/150)

BRENDA support

Ligand calcidiol

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.

Basic Ligand Information

Molecular Structure
Picture of calcidiol (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
C27H44O2
calcidiol
JWUBBDSIWDLEOM-DTOXIADCSA-N
Synonyms:
25-hydroxy-vitamin D3, 25-hydroxy-[3alpha-3H]vitamin D3, 25-hydroxy-[26,27-3H]vitamin D3, 25-hydroxycholecalciferol, 25-hydroxyvitamin D, 25-hydroxyvitamin D3


Show all pahtways known for Show all BRENDA pathways known for calcidiol

Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (9 results)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE

In Vivo Product in Enzyme-catalyzed Reactions (5 results)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
calciol + O2 + [reduced NADPH-hemoprotein reductase] = calcidiol + [oxidized NADPH-hemoprotein reductase] + H2O
show the reaction diagram
-

Substrate in Enzyme-catalyzed Reactions (18 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
25-hydroxyvitamin D3 + O2 + NADPH + H+ = 1alpha,25-dihydroxyitamin D3 + NADP+ + H2O
show the reaction diagram
-

Product in Enzyme-catalyzed Reactions (11 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
cholecalciferol + H2O2 = 25-hydroxycholecalciferol + 24-hydroxycholecalciferol + 26,27-hydroxycholecalciferol + H2O
show the reaction diagram
-

Inhibitor in Enzyme-catalyzed Reactions (2 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
CYP27B1 shows inhibition when substrate concentrations in the membrane are greater than 4 times Km, more pronounced with 25-hydroxyvitamin D3 than with 25-hydroxyvitamin D2
-

Enzyme Kinetic Parameters

kcat Value (Turnover Number) (10 results)

EC NUMBER
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
COMMENTARY
LITERATURE
0.002
-
in 20 mM Tris (pH 7.5), at 37°C
0.0023
-
1alpha-hydroxylation, mutant R73V/R84A, pH not specified in the publication, temperature not specified in the publication

KM Value (20 results)

EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
0.0071
-
30°C, pH not specified in the publication

References & Links

Links to other databases for calcidiol

ChEBI
PubChem
ChEBI
PubChem