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Ligand isocitric acid

Basic Ligand Information

Molecular Structure

Pathway Source

Pathways

BRENDA

citric acid cycle, methylaspartate cycle

KEGG

2-Oxocarboxylic acid metabolism, Biosynthesis of alkaloids derived from histidine and purine, Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid, Biosynthesis of alkaloids derived from shikimate pathway, Biosynthesis of alkaloids derived from terpenoid and polyketide, Biosynthesis of amino acids, Biosynthesis of cofactors, Biosynthesis of phenylpropanoids, Biosynthesis of plant hormones, Biosynthesis of plant secondary metabolites, Biosynthesis of secondary metabolites, Biosynthesis of terpenoids and steroids, Carbon fixation pathways in prokaryotes, Carbon metabolism, Central carbon metabolism in cancer, Citrate cycle (TCA cycle), Glucagon signaling pathway, Glyoxylate and dicarboxylate metabolism, Microbial metabolism in diverse environments more

Show all BRENDA pathways known for isocitric acid

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Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (15 results)

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Show Natural Substrate (15 entries)

EC NUMBER

PROVEN IN VIVO REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

1.1.1.286

isocitrate + NAD+ = 2-oxoglutarate + CO2 + NADH + H+

740000, 740021

-

1.1.1.286

isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH + H+

738195

-

1.1.1.41

DL-isocitrate + NAD+ = 2-oxoglutarate + CO2 + NADH

656052, 723607

-

1.1.1.41

DL-isocitrate + NAD+ = 2-oxoglutarate + CO2 + NADH + H+

761866

-

1.1.1.41

isocitrate + NAD+ = 2-oxoglutarate + CO2 + NADH + H+

286749, 286773, 286777, 762238, 286769, 740114, 286767, 286751, 286755, 286759, 286775, 286770, 286771, 286772, 721433, 286752, 286757, 286758, 286760, 286762, 286764, 286765, 286768, 286753, 286776, 286774, 654914, 286761, 286756, 654783, 286754, 286750, 286766, 722295, 286763, 761311, 762313, 762222, 655607, 740425, 760649, 760747, 741414, 721381, 741236, 741458, 741407, 760834, 762407, 762437, 762451, 761502, 654652, 656118, 657111, 654843

-

1.1.1.41

isocitrate + NAD+ = 2-oxoglutarate + NADH + H+ + CO2

712440, 713380, 713076, 712180

-

1.1.1.42

DL-isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH

654649

-

1.1.1.42

DL-isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH + H+

761866, 723151, 723152, 723129

-

1.1.1.42

isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH + H+

739770, 740946, 657309, 654652, 740181, 654914, 719515, 760653, 761559, 655142, 760747, 740721, 762409, 761531, 740117, 760832, 761047, 762446, 740583, 760535, 761477, 761677, 761403, 656093, 727835, 721687, 721162, 722338, 722204, 721312, 760361, 722896, 727545, 740185, 741388, 740551, 739874, 761055, 762026, 762222, 762498, 762504, 760369, 760487, 761052, 761784, 655430, 656247, 656233, 654367, 655035, 656616, 656740, 654843, 684705, 686895, 726802

-

1.1.1.42

isocitrate + NADP+ = 2-oxoglutarate + NADPH + H+ + CO2

286779, 286806, 286780, 286786, 286790, 286801, 286804, 711158, 711330, 713508, 713113, 286812, 286782, 286797, 286749, 711006, 286783, 286785, 286788, 286784, 286793, 286794, 286800, 286810, 713246, 286781, 286791, 286795, 286813, 286796, 286787, 286802, 286811, 286803, 286789, 286799, 286792, 286798, 286807, 286805, 286809, 711092, 711107, 712614, 713100, 711119, 286808, 712180

-

1.1.1.42

isocitrate + NADP+ = oxalosuccinate + NADPH + H+

739990, 741193

-

1.1.1.286

2 entries

1.1.1.41

4 entries

1.1.1.42

5 entries

1.1.1.87

isocitrate + NAD+ = ?

660907

-

2.3.1.126

caffeoyl-CoA + isocitrate = CoA + 2-caffeoylisocitrate

486247

-

4.1.3.1

isocitrate = ?

33147, 33148, 33130, 33131, 33138, 33115, 33116, 33117, 33133, 33141, 33137, 33154, 33152, 33124, 33149, 33140, 33129, 33135, 33132, 33155, 33125, 33123, 33126, 33153, 33145

-

4.1.3.1

isocitrate = succinate + glyoxylate

746757, 747240, 653939, 651395, 747975, 728417, 727419, 651648, 728701, 747939, 748283, 748738, 727039, 727045, 653476, 747309, 748483, 748756, 653514, 650574, 748480, 650913, 638504, 651215, 650335, 728845, 747203, 749329, 727196, 748505, 748615, 748632, 653163, 649253, 649361, 649454, 727500

-

4.1.3.1

2 entries

In Vivo Product in Enzyme-catalyzed Reactions (2 results)

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Show Natural Product (2 entries)

EC NUMBER

PROVEN IN VIVO REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

1.1.1.42

2-oxoglutarate + CO2 + NADPH + H+ = isocitrate + NADP+

0

-

4.2.1.3

cis-aconitate + H2O = isocitrate

0

-

Substrate in Enzyme-catalyzed Reactions (43 results)

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Show Substrate (43 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

1.1.1.286

isocitrate + NAD+ = 2-oxoglutarate + CO2 + NADH + H+

660918, 740000, 740021, 656127, 660907, 696220, 685232

-

1.1.1.286

isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH + H+

738195, 660907, 660918

-

1.1.1.41

DL-isocitrate + NAD+ = 2-oxoglutarate + CO2 + NADH

656052, 723607, 740425, 700359

-

1.1.1.41

DL-isocitrate + NAD+ = 2-oxoglutarate + CO2 + NADH + H+

761866

-

1.1.1.41

DL-isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH

740425

-

1.1.1.41

8-(4-bromo-2,3-dioxobutylthio)-NAD+ + isocitrate = 2-oxoglutarate + CO2 + 8-(4-bromo-2,3-dioxobutylthio)-NADH

286775

-

1.1.1.41

isocitrate + APAD+ = 2-oxoglutarate + CO2 + APADH

762222

-

1.1.1.41

isocitrate + NAD+ = 2-oxoglutarate + CO2 + NADH + H+

286749, 286773, 286777, 762238, 286769, 669477, 685154, 740114, 286767, 286751, 286755, 286759, 286775, 286770, 286771, 286772, 654652, 721433, 286752, 286757, 286758, 286760, 286762, 286764, 286765, 286768, 286753, 286776, 286774, 654914, 286761, 286756, 654783, 286754, 286750, 286766, 722295, 286763, 761311, 762313, 660907, 762222, 655607, 740425, 760649, 760747, 667332, 741414, 721381, 741236, 741458, 741407, 760834, 762407, 762437, 762451, 761502, 656118, 657111, 654843, 684749, 721450, 727835

-

1.1.1.41

isocitrate + NAD+ = 2-oxoglutarate + NADH + H+ + CO2

712180, 712440, 713380, 713076

-

1.1.1.41

isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH + H+

286777, 670158, 655607, 760747, 741414, 722295, 721450, 741236, 762313, 760649

-

1.1.1.41

isocitrate + NHD+ = 2-oxoglutarate + CO2 + NHDH

762222

-

1.1.1.42

DL-isocitrate + NAD+ = 2-oxoglutarate + CO2 + NADH

667326, 670805

-

1.1.1.42

DL-isocitrate + NAD+ = 2-oxoglutarate + CO2 + NADH + H+

723151

-

1.1.1.42

DL-isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH

700359, 670236, 654649, 668080, 655094, 655035, 655604, 667326, 669373, 670805

-

1.1.1.42

DL-isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH + H+

761866, 723151, 723152, 723129

-

1.1.1.42

Ds-isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH

654649

-

1.1.1.42

isocitrate + APADP+ = 2-oxoglutarate + CO2 + APADPH

762222

-

1.1.1.42

isocitrate + NAD+ = 2-oxoglutarate + CO2 + NADH + H+

286802, 760747, 286808, 719515, 740551, 739874, 761055

-

1.1.1.42

isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH + H+

655430, 656740, 696487, 680481, 698056, 655663, 656233, 698058, 698059, 739770, 740946, 656431, 654341, 657309, 654652, 684709, 740181, 654914, 654843, 654783, 719515, 656616, 760653, 761559, 655142, 760747, 686899, 740721, 762409, 761531, 688373, 670236, 740117, 760832, 761047, 762446, 740583, 699040, 760535, 761477, 761677, 696416, 722205, 761403, 656093, 656180, 727835, 721687, 721162, 722338, 722204, 721312, 760361, 722896, 727432, 728133, 727545, 740185, 741388, 740551, 739874, 761055, 762026, 762222, 762498, 762504, 760369, 760487, 761052, 761784, 656247, 654367, 655035, 684705, 686895, 726802

-

1.1.1.42

isocitrate + NADP+ = 2-oxoglutarate + NADPH + H+ + CO2

286779, 286806, 286780, 286786, 286790, 286801, 286804, 711158, 711330, 712180, 713508, 713113, 286812, 286782, 286797, 286749, 711006, 286783, 286785, 286788, 286784, 286793, 286794, 286800, 286810, 713246, 286781, 286791, 286795, 286813, 286796, 286787, 286802, 286811, 286803, 286789, 286799, 286792, 286798, 286807, 286805, 286809, 711092, 711107, 712614, 713100, 711119, 286808

-

1.1.1.42

isocitrate + NADP+ = oxalosuccinate + NADPH + H+

739990, 741193

-

1.1.1.42

isocitrate + NHDP+ = 2-oxoglutarate + CO2 + NHDPH

762222

-

1.1.1.87

1-hydroxy-1,2,3-propanetricarboxylate + NAD+ = ?

656127

-

1.1.1.87

isocitrate + NAD+ = ?

685232, 660918, 667934, 660907, 669109

-

1.1.1.87

isocitrate + NAD+ = ? + NADH + H+

696220

-

1.1.1.87

isocitrate + NADP+ = 2-oxoglutarate + NADPH + H+ + CO2

760522

-

1.1.1.286

2 entries

1.1.1.41

9 entries

1.1.1.42

11 entries

1.1.1.87

4 entries

2.1.1.144

S-adenosyl-L-methionine + DL-isocitrate = S-adenosyl-L-homocysteine + ?

288662, 288663

-

2.3.1.126

4-coumaroyl-CoA + isocitrate = CoA + 4-coumaroylisocitrate

486247

-

2.3.1.126

caffeoyl-CoA + isocitrate = CoA + 2-caffeoylisocitrate

486247

-

2.3.1.126

caffeoyl-CoA + isocitric acid = CoA + (E)-caffeoylisocitric acid

486247

-

2.3.1.126

feruloyl-CoA + isocitric acid = CoA + feruloylisocitric acid

486247

-

2.3.1.126

sinapoyl-CoA + isocitric acid = CoA + sinapoylisocitric acid

486247

-

4.1.3.1

D,L-isocitrate = succinate + glyoxylate

663463

-

4.1.3.1

DL-isocitrate = succinate + glyoxylate

666901, 748480, 748615

-

4.1.3.1

isocitrate + phenylhydrazine = ? + glyoxylate phenylhydrazone

653163

-

4.1.3.1

isocitrate = ?

33147, 33148, 33130, 33131, 33138, 33115, 33116, 33117, 33133, 33141, 33137, 33154, 33152, 33124, 33149, 33140, 33129, 33135, 33132, 33155, 33125, 33123, 33126, 33153, 33145

-

4.1.3.1

isocitrate = glyoxylate + succinate

713812, 666268, 714980, 700883

-

4.1.3.1

isocitrate = succinate + glyoxylate

33138, 33130, 33131, 680497, 746757, 704605, 33146, 649253, 680481, 747240, 33115, 33116, 33117, 33118, 33151, 33133, 33141, 653939, 33137, 705514, 651395, 747975, 33147, 33154, 33152, 33124, 705567, 728417, 33149, 649361, 33140, 679815, 727419, 651648, 679472, 728701, 747939, 748283, 748738, 727039, 727045, 653476, 663767, 33129, 747309, 33134, 33135, 748483, 677867, 748756, 33132, 33119, 33120, 673985, 33155, 33148, 653163, 33139, 33125, 678657, 680310, 679978, 680135, 33121, 653514, 33123, 650574, 33126, 33127, 33128, 33136, 33153, 748480, 650913, 638504, 651215, 650335, 649454, 704000, 658479, 715749, 664147, 665178, 670169, 679717, 679906, 728845, 681995, 747203, 749329, 33145, 715548, 727196, 748505, 748615, 748632, 33122, 701127, 698008, 702178, 727500

-

4.2.1.3

isocitrate = ?

651095, 652036

-

4.2.1.3

isocitrate = cis-aconitate + H2O

730343, 704318, 33812, 33814, 649675, 666706, 33784, 704735, 33811, 33816, 33794, 33803, 715699, 680491

-

4.2.1.3

isocitrate = citrate

33784, 33797, 33788, 748481, 33792, 33793, 33786

-

2.3.1.126

5 entries

4.1.3.1

6 entries

4.2.1.3

3 entries

4.2.1.79

DL-isocitrate = ?

653158

-

6.2.1.9

ATP + DL-isocitrate + CoA = ADP + phosphate + isocitryl-CoA

856

-

Product in Enzyme-catalyzed Reactions (5 results)

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Show Product (5 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

1.1.1.42

2-oxoglutarate + CO2 + NADPH + H+ = isocitrate + NADP+

0

-

1.1.1.42

2-oxoglutarate + CO2 + NADPH = isocitrate + NADP+

0

-

1.1.1.42

2 entries

4.1.3.1

succinate + glyoxylate = isocitrate

0

-

4.2.1.3

cis-aconitate + H2O = isocitrate

0, 33784, 33808, 33811

-

4.2.1.3

citrate = isocitrate

0, 33784, 33811, 33786

-

4.2.1.3

2 entries

Activator in Enzyme-catalyzed Reactions (15 results)

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Show Activating Compound (15 entries)

EC NUMBER

COMMENTARY

LITERATURE

ENZYME 3D STRUCTURE

1.1.1.36

25% activation, stimulates NADP+ regeneration

286622

-

1.1.1.37

oxidation of malate to oxaloacetate

656320

-

1.1.1.42

isozymes ICDH2 and ICDH1

656616

-

1.1.3.15

-

677109

-

1.1.3.15

strong, bundle sheath isoform, slight, mesophyll isoform

656622

-

1.1.3.15

2 entries

1.3.5.1

-

723986

-

1.4.1.2

10 M, 112% of initial activity

742464

-

2.3.3.5

1 mM, relative activity 101%

488166

-

2.7.1.1

activates isoenzyme Hex II

640232

-

2.7.4.3

activation

642602

-

3.2.1.62

1.7fold stimulation only for phlorizin activity

208763

-

4.1.2.4

activates

5168

-

4.3.3.7

activation

33906

-

6.4.1.2

maximal activation at 3 mM

37594

-

6.4.1.2

nearly absolute requirement for tricarboxylate activators such as citrate or isocitrate

37586

-

6.4.1.2

2 entries

Inhibitor in Enzyme-catalyzed Reactions (45 results)

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Show Inhibitors (45 entries)

EC NUMBER

COMMENTARY

LITERATURE

ENZYME 3D STRUCTURE

1.1.1.27

-

286447

-

1.1.1.286

competitive, pH-dependent substrate inhibition

685232

-

1.1.1.37

reduction of oxaloacetate to malate

656320

-

1.1.1.39

-

286720

-

1.1.1.42

50% inhibition of the reverse reaction at 0.1 mM, 97% inhibition at 1.2 mM

656740

-

1.1.1.42

70% inhibition of the reverse reaction at 0.1 mM, 99% inhibition at 1.2 mM

656740

-

1.1.1.42

competitive inhibition of decarboxylation of oxalosuccinate

286749

-

1.1.1.42

reverse reaction

654843

-

1.1.1.42

4 entries

1.1.1.49

-

286997

-

1.1.1.82

5 mM

287420

-

1.1.1.85

-

639155

-

1.14.11.2

competitive inhibition with respect to 2-oxoglutarate

439205

-

1.14.11.8

-

7020

-

1.4.1.13

32% inhibition at 20 mM

391455

-

1.4.1.13

35% inhibition at 5 mM

391440

-

1.4.1.13

35% inhibition at 50 mM

391438

-

1.4.1.2

-

391553, 391560

-

1.4.1.2

49% activity in the presence of 10 mM isocitrate

692832

-

1.4.1.13

3 entries

1.4.1.2

2 entries

1.4.1.4

10 M, 64% of initial activity

742464

-

2.3.3.8

-

488189

-

2.6.1.1

-

672283

-

2.7.1.106

-

640350

-

2.7.1.11

brain, not heart

640421

-

2.7.1.11

competitive inhibition

640507

-

2.7.1.11

mild inhibitory effects, but only at concentrations exceeding 2 mM

722202

-

2.7.1.40

10 mM, 70% of activity remaining; 10 mM, 75% of activity remaining

676434

-

2.7.1.40

-

641516, 641587, 641612

-

2.7.1.40

30% inhibition at 5 mM

723044

-

2.7.1.40

76% activity at pH 6.4, 91% activity at pH 7.4

676689

-

2.7.1.40

not

641565

-

2.7.1.40

only isozyme PKp, not PKc

641576

-

2.7.1.11

3 entries

2.7.1.40

6 entries

2.7.11.5

inhibits only kinase activity

640602, 640609

-

2.7.7.27

-

643135

-

2.8.1.1

-

645511

-

3.1.3.11

-

749502

-

4.1.1.31

-

655409

-

4.1.1.31

-

4307

-

4.1.1.31

2 entries

4.1.1.32

above 5.0 mM, weak

4350

-

4.1.1.71

-

648367

-

4.1.3.30

-

714846

-

4.2.1.2

L-isocitrate

33728

-

5.3.2.2

inhibits enzyme form OAT-2

2878

-

5.4.2.2

weak inhibition

650261

-

5.4.2.9

-

648835

-

5.5.1.2

noncompetitive, localization of binding pocket on the enzyme outside the active site

679754

-

3D Structure of Enzyme-Ligand-Complex (PDB) (42 results)

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Show 3D Structure of Enzyme-Ligand-Complex (PDB) (42 entries)

EC NUMBER

ENZYME 3D STRUCTURE

1.1.1.41

5grl, 3D-view

1.1.1.41

7e2w, 3D-view

1.1.1.42

1ai3, 3D-view

1.1.1.42

1cw1, 3D-view

1.1.1.42

1cw7, 3D-view

1.1.1.42

1gro, 3D-view

1.1.1.42

1grp, 3D-view

1.1.1.42

1ide, 3D-view

1.1.1.42

1itw, 3D-view

1.1.1.42

1lwd, 3D-view

1.1.1.42

1p8f, 3D-view

1.1.1.42

1pb1, 3D-view

1.1.1.42

1t0l, 3D-view

1.1.1.42

1xkd, 3D-view

1.1.1.42

2qfw, 3D-view

1.1.1.42

3map, 3D-view

1.1.1.42

3mas, 3D-view

1.1.1.42

4aj3, 3D-view

1.1.1.42

4aja, 3D-view

1.1.1.42

4ajb, 3D-view

1.1.1.42

4aou, 3D-view

1.1.1.42

4aov, 3D-view

1.1.1.42

4bnp, 3D-view

1.1.1.42

4zda, 3D-view

1.1.1.42

5h3e, 3D-view

1.1.1.42

5h3f, 3D-view

1.1.1.42

5icd, 3D-view

1.1.1.42

5yfn, 3D-view

1.1.1.42

6ajc, 3D-view

1.1.1.42

6bkx, 3D-view

1.1.1.42

6bl0, 3D-view

1.1.1.42

6bl1, 3D-view

1.1.1.42

6bl2, 3D-view

1.1.1.42

6pay, 3D-view

1.1.1.42

7e3n, 3D-view

1.1.1.42

8icd, 3D-view

1.1.1.41

2 entries

1.1.1.42

34 entries

3.5.2.6

1w7f, 3D-view

4.1.3.1

3p0x, 3D-view

4.1.3.30

1xg4, 3D-view

4.2.1.3

1b0j, 3D-view

4.2.1.3

1c97, 3D-view

4.2.1.3

7acn, 3D-view

4.2.1.3

3 entries

Enzyme Kinetic Parameters

k_cat Value (Turnover Number) (146 results)

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Show Turnover Number (146 entries)

EC NUMBER

TURNOVER NUMBER [1/S]

TURNOVER NUMBER MAXIMUM [1/S]

COMMENTARY

LITERATURE

1.1.1.286

0.22

-

pH 8.0, 60°C, recombinant His6-tagged mutant T71V

740021

1.1.1.286

0.71

-

pH 8.0, 60°C, recombinant His6-tagged mutant S80A

740021

1.1.1.286

1

-

pH 8.0, 60°C, recombinant His6-tagged mutant I82N

740021

1.1.1.286

1.1

-

pH 8.0, 60°C, recombinant His6-tagged mutant L81P

740021

1.1.1.286

5

-

pH 8.0, 60°C, recombinant His6-tagged wild-type enzyme

740021

1.1.1.286

6.6

-

pH 8.0, 60°C, recombinant His6-tagged mutant L83R

740021

1.1.1.286

10.9

-

30°C, pH 7.8, A80 deletion mutant

660918

1.1.1.286

14.8

-

70°C, pH 7.8

660907

1.1.1.286

42.8

-

30°C, pH 7.8, wild-type

660918

1.1.1.286

61

-

pH 8.0, 60°C, recombinant enzyme

738195

1.1.1.286

76

-

pH 8.0, 60°C, recombinant enzyme

740000

1.1.1.286

171

-

60°C, pH 8.0

656127

1.1.1.41

0.27

-

25°C, pH 7.2, alpha-subunit mutant enzyme D234C

669477

1.1.1.41

1.5

-

pH 7.5, 37°C, recombinant enzyme mutant S102Y

723607

1.1.1.41

2.49

-

25°C, pH 7.2, alpha-subunit mutant enzyme D230C

669477

1.1.1.41

3.3

-

pH 7.5, 37°C, recombinant enzyme mutant S102A

723607

1.1.1.41

4

-

pH 7.5, 37°C, recombinant enzyme mutant S102G

723607

1.1.1.41

11.8

-

25°C, pH 7.2, gamma-subunit mutant enzyme D215N

669477

1.1.1.41

14.8

-

6fold His-tagged enzyme, at 70°C in 50 mM N-2-hydroxyethylpiperadine-N0-2-ethanesulfonate-NaOH (pH 7.8), 0.2 M KCl, 0.2 mM MnCl2, 1 mM NAD+

660907

1.1.1.41

19.6

-

25°C, pH 7.2, beta-subunit mutant enzyme D217N

669477

1.1.1.41

25

-

pH 7.5, 37°C, recombinant enzyme mutant S102T

723607

1.1.1.41

30

-

25°C, pH 7.2, wild-type enzyme

669477

1.1.1.41

36.8

-

25°C, pH 7.2, alpha-subunit mutant enzyme D206N

669477

1.1.1.41

37

-

mutant enzyme L580H/L591R/A640R, at pH 7.5 and 25°C

760649

1.1.1.41

78.98

-

at pH 7.4 and 37°C

761866

1.1.1.41

85

-

wild type enzyme, at pH 7.5 and 25°C

760649

1.1.1.41

124

-

pH 7.5, 37°C, recombinant wild-type enzyme

723607

1.1.1.42

0.0005

-

mutant R291S/Y390P, pH 8.0, 30°C

670805

1.1.1.42

0.0013

-

mutant R291S/K343D/Y390P, pH 8.0, 30°C

670805

1.1.1.42

0.002

-

mutant R291S, pH 8.0, 30°C

670805

1.1.1.42

0.0023

-

wild-type, pH 8.0, 30°C

670805

1.1.1.42

0.0025

-

mutant R291S/K343D/Y344I/Y390P, pH 8.0, 30°C

670805

1.1.1.42

0.005

-

mutant R291S/K343D/Y344I/V350A/Y390P, pH 8.0, 30°C

670805

1.1.1.42

0.047

-

mutant enzyme R132N, at pH 7.5 and 37°C

761477

1.1.1.42

0.12

-

mutant enzyme R132H, at pH 7.5 and 21°C

761477

1.1.1.42

0.18

-

mutant enzyme R153H, at pH 7.5 and 25°C

761403

1.1.1.42

0.2

-

mutant enzyme A134D, at pH 7.5 and 21°C

761477

1.1.1.42

0.3

-

mutant enzyme R153C, at pH 7.5 and 25°C

761403

1.1.1.42

1

-

mutant enzyme R132G, at pH 7.5 and 21°C

761477

1.1.1.42

1.21

-

mutant enzyme R132W, at pH 7.5 and 37°C

761477

1.1.1.42

1.4

-

mutant enzyme R100Q, at pH 7.5 and 21°C

761477

1.1.1.42

1.61

-

mutant enzyme R132C, at pH 7.5 and 21°C

761477

1.1.1.42

1.9

-

isoform ICD-1, presence of Zn2+, pH 7.5, 25°C

668080

1.1.1.42

2.1

-

pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S113A

760653

1.1.1.42

2.3

-

mutant enzyme A134D, at pH 7.5 and 37°C

761477

1.1.1.42

2.4

-

mutant enzyme R132H, at pH 7.5 and 37°C

761477

1.1.1.42

2.66

-

mutant K256Q of isoform IDH2, at pH 7.4 and 25°C

762446

1.1.1.42

3

6

pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S22E

760653

1.1.1.42

3.2

-

pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S113T

760653

1.1.1.42

3.8

-

isoform ICD-1, presence of Mg2+, pH 7.5, 25°C

668080

1.1.1.42

4

-

pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S113G

760653

1.1.1.42

4.31

-

mutant enzyme C3636S, at pH 7.5 and 25°C

760361

1.1.1.42

4.4

-

mutant enzyme R132C, at pH 7.5 and 37°C

761477

1.1.1.42

4.5

-

5°C

688373

1.1.1.42

4.61

-

mutant K413Q of isoform IDH2, at pH 7.4 and 25°C

762446

1.1.1.42

4.9

-

mutant enzyme K413Q, at pH 8.0 and 37°C

762409

1.1.1.42

4.93

-

wild type enzyme, at pH 7.5 and 25°C

760361

1.1.1.42

5.6

-

mutant enzyme R100Q, at pH 7.5 and 37°C

761477

1.1.1.42

5.6

-

wild type isoform IDH2, at pH 7.4 and 25°C

762446

1.1.1.42

7

-

mutant enzyme D273L, in potassium phosphate buffer, at pH 7.5 and 37°C

760535

1.1.1.42

7.2

-

mutant enzyme R132K, at pH 7.5 and 37°C

761477

1.1.1.42

9.2

-

mutant enzyme R132Q, at pH 7.5 and 37°C

761477

1.1.1.42

9.3

-

mutant enzyme R132G, at pH 7.5 and 37°C

761477

1.1.1.42

9.4

-

mutant enzyme H133Q, at pH 7.5 and 21°C

761477

1.1.1.42

10.29

-

with Mg2+, pH 7.5, 50°C

723152

1.1.1.42

10.4

-

mutant enzyme R132A, at pH 7.5 and 37°C

761477

1.1.1.42

10.92

-

with Mg2+, pH 7.5, 37°C

723152

1.1.1.42

11

-

wild type enzyme, at pH 7.5 and 21°C

761477

1.1.1.42

15

-

mutant enzyme D273S, in potassium phosphate buffer, at pH 7.5 and 37°C

760535

1.1.1.42

15.5

-

wild type enzyme, at pH 8.0 and 37°C

762409

1.1.1.42

19.6

-

isoform ICD-2, presence of Mg2+, pH 7.5, 25°C

668080

1.1.1.42

20

-

wild type enzyme, in Bis-Tris buffer, at pH 6.2 and 37°C

760535

1.1.1.42

20.2

-

wild type enzyme, in potassium phosphate buffer, at pH 6.2 and 37°C

760535

1.1.1.42

22

-

mutant enzyme D273N, in potassium phosphate buffer, at pH 7.5 and 37°C

760535

1.1.1.42

23

-

wild type enzyme, in potassium phosphate buffer, at pH 6.5 and 37°C

760535

1.1.1.42

23.1

-

wild type enzyme, in Bis-Tris buffer, at pH 6.5 and 37°C

760535

1.1.1.42

27.5

-

wild type enzyme, in potassium phosphate buffer, at pH 6.8 and 37°C

760535

1.1.1.42

28.7

-

mutant enzyme K217Q, in potassium phosphate buffer, at pH 7.5 and 37°C

760535

1.1.1.42

31.3

-

pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S22A

760653

1.1.1.42

31.4

-

25°C

688373

1.1.1.42

31.7

-

wild type enzyme, in potassium phosphate buffer, at pH 7.0 and 37°C

760535

1.1.1.42

32

-

wild type enzyme, in Bis-Tris buffer, at pH 7.0 and 37°C

760535

1.1.1.42

33

-

pH 7.5, temperature not specified in the publication, recombinant His-tagged enzyme

721687

1.1.1.42

33.4

-

pH 8.0, 25°C, in presence of Mg2+, wild-type enzyme

760653

1.1.1.42

35.4

-

wild type enzyme, in Tris buffer, at pH 7.8 and 37°C

760535

1.1.1.42

35.5

-

wild type enzyme, in potassium phosphate buffer, at pH 7.2 and 37°C

760535

1.1.1.42

37

-

pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S22H

760653

1.1.1.42

37.6

-

wild type enzyme, in potassium phosphate buffer, at pH 7.5 and 37°C

760535

1.1.1.42

38.3

-

wild type enzyme, in Tris buffer, at pH 8.0 and 37°C

760535

1.1.1.42

39

-

wild type enzyme, in Tris buffer, at pH 7.2 and 37°C

760535

1.1.1.42

39.2

-

wild type enzyme, in potassium phosphate buffer, at pH 8.0 and 37°C

760535

1.1.1.42

40

-

wild type enzyme, in potassium phosphate buffer, at pH 7.8 and 37°C

760535

1.1.1.42

40.4

-

wild type enzyme, in Tris buffer, at pH 7.5 and 37°C

760535

1.1.1.42

42

-

mutant enzyme K217M, in potassium phosphate buffer, at pH 7.5 and 37°C

760535

1.1.1.42

43.21

-

with Mn2+, pH 7.5, 37°C

723152

1.1.1.42

43.21

-

with Mn2+, pH 7.5, 50°C

723152

1.1.1.42

45

-

mutant enzyme H133Q, at pH 7.5 and 37°C

761477

1.1.1.42

50.4

-

pH 8.0, 25°C, in presence of Mn2+, wild-type enzyme

760653

1.1.1.42

51.8

-

wild type enzyme, at pH 7.5 and 25°C

761403

1.1.1.42

58.3

-

oxidative decarboxylation

286749

1.1.1.42

69.88

-

wild type enzyme, at pH 8.0 and 20°C

761559

1.1.1.42

82.47

-

mutant enzyme A741S, at pH 8.0 and 20°C

761559

1.1.1.42

83.03

-

at pH 7.4 and 37°C

761866

1.1.1.42

85

-

wild type enzyme, at pH 7.5 and 37°C

761477

1.1.1.42

89.04

-

mutant enzyme E596L, at pH 8.0 and 20°C

761559

1.1.1.42

102.98

-

at pH 8.0 and 20°C

761052

1.1.1.42

106.4

-

40°C, pH 8.0

722205

1.1.1.42

136.85

-

wild type enzyme, at pH 8.0 and 20°C

761559

1.1.1.42

158.91

-

mutant enzyme L594E, at pH 8.0 and 20°C

761559

1.1.1.42

255

-

60°C, pH 8.0

722205

1.1.1.87

0.22

-

mutant enzyme T71V, with NAD+ as cosubstrate, at pH 8.0 and 60°C

760522

1.1.1.87

0.71

-

mutant enzyme S80A, with NAD+ as cosubstrate, at pH 8.0 and 60°C

760522

1.1.1.87

1

-

mutant enzyme I82N, with NAD+ as cosubstrate, at pH 8.0 and 60°C

760522

1.1.1.87

1.1

-

mutant enzyme L81P, with NAD+ as cosubstrate, at pH 8.0 and 60°C

760522

1.1.1.87

5

-

wild type enzyme, with NAD+ as cosubstrate, at pH 8.0 and 60°C

760522

1.1.1.87

6.6

-

mutant enzyme L83R, with NAD+ as cosubstrate, at pH 8.0 and 60°C

760522

1.1.1.87

10.9

-

pH 7.8, 25°C, recombinant mutant A80del

660918

1.1.1.87

14.8

-

pH 7.8, 70°C, recombinant enzyme

660907

1.1.1.87

42.8

-

pH 7.8, 25°C, recombinant wild-type enzyme

660918

1.1.1.87

43

-

pH 7.8, 28°C, recombinant enzyme

667934

1.1.1.87

171

-

purified enzyme, pH 8.0, 60°C

656127

1.1.1.87

211

-

pH 7.5, 60°C, recombinant mutant V135M

669109

1.1.1.87

438

-

pH 7.5, 60°C, recombinant wild-type enzyme

669109

4.1.3.1

0.56

-

recombinant His-tagged mutant N214D, pH 7.8, 30°C

728845

4.1.3.1

1.38

-

ICL2, in Tricine-HCl pH 7.5, 5 mM MgCl2

681995

4.1.3.1

3.25

-

recombinant His-tagged mutant Q221K, pH 7.8, 30°C

728845

4.1.3.1

3.46

-

recombinant His-tagged mutant Q211H, pH 7.8, 30°C

728845

4.1.3.1

4.4

-

mutant enzyme T534A, at pH 7.0 and 37°C

748505

4.1.3.1

4.63

-

recombinant His-tagged mutant E219A, pH 7.8, 30°C

728845

4.1.3.1

5.2

-

pH 7.5, 25°C, recombinant enzyme

727039

4.1.3.1

5.24

-

ICL1, in 50 mM MOPS pH 6.8, 5 mM MgCl2

681995

4.1.3.1

8.43

-

in 50 mM MOPS buffer, pH 7.5, with 1 mM MgCl2, 10 mM 2-mercaptoethanol, and 2.7 mM O-nitrophenyl-beta-D-galactopyranoside

679978

4.1.3.1

9.81

-

recombinant His-tagged wild-type enzyme, pH 7.8, 30°C

728845

4.1.3.1

12.2

-

recombinant detagged enzyme, pH 7.0, 37°C

727045

4.1.3.1

13.3

-

mutant enzyme A341N, at 25°C

679717

4.1.3.1

15.9

-

mutant enzyme T534V, at pH 7.0 and 37°C

748505

4.1.3.1

17.6

-

wild type enzyme, at pH 7.0 and 37°C

748505

4.1.3.1

18.1

-

20°C, pH 6.8

638504

4.1.3.1

24

-

-

33133

4.1.3.1

26.6

-

mutant enzyme Q47K, at 25°C

679717

4.1.3.1

28.5

-

wild type enzyme, at 25°C

679717

4.1.3.1

32

-

pH 7

649454

4.1.3.1

44.5

-

-

33123

4.1.3.1

122

-

-

33128

4.1.3.1

800

-

-

33136

1.1.1.286

12 entries

1.1.1.41

15 entries

1.1.1.42

83 entries

1.1.1.87

13 entries

4.1.3.1

22 entries

4.2.1.3

1.1

-

pH 8, 25°C, Lineweaver-Burk method

730343

K_M Value (331 results)

top print hide

Show KM Value (331 entries)

EC NUMBER

KM VALUE [MM]

KM VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

1.1.1.286

0.014

-

pH 8.0, 60°C, recombinant His6-tagged wild-type enzyme

740021

1.1.1.286

0.0164

-

70°C, pH 7.8

660907

1.1.1.286

0.027

-

pH 8.0, 60°C, recombinant enzyme

738195

1.1.1.286

0.29

-

pH 8.0, 60°C, recombinant enzyme

740000

1.1.1.286

0.291

-

30°C, pH 7.8, wild-type

660918

1.1.1.286

0.3

-

pH 8.0, 60°C, recombinant His6-tagged mutant S80A

740021

1.1.1.286

0.405

-

60°C, pH 8.0

656127

1.1.1.286

0.465

-

30°C, pH 7.8, A80 deletion mutant

660918

1.1.1.286

0.5

-

pH 8.0, 60°C, recombinant His6-tagged mutant T71V

740021

1.1.1.286

0.58

-

pH 8.0, 60°C, recombinant His6-tagged mutant L83R

740021

1.1.1.286

1.2

-

pH 8.0, 60°C, recombinant His6-tagged mutant L81P

740021

1.1.1.286

2.2

-

pH 8.0, 60°C, recombinant His6-tagged mutant I82N

740021

1.1.1.41

0.002

-

at pH 7.4 and 37°C

761866

1.1.1.41

0.009

-

at pH 8.5 and 42°C

762222

1.1.1.41

0.0164

-

6fold His-tagged enzyme, at 70°C in 50 mM N-2-hydroxyethylpiperadine-N0-2-ethanesulfonate-NaOH (pH 7.8), 0.2 M KCl, 0.2 mM MnCl2, 1mM NAD+

660907

1.1.1.41

0.03

-

-

286750

1.1.1.41

0.031

-

mutant enzyme L580H/L591R/A640R, at pH 7.5 and 25°C

760649

1.1.1.41

0.038

-

wild type enzyme, at pH 7.5 and 25°C

760649

1.1.1.41

0.0517

-

-

684749

1.1.1.41

0.075

-

pH 7.5, 37°C, recombinant wild-type enzyme

723607

1.1.1.41

0.09

-

pH 7.2, 25°C, recombinant mutant betaY137E, in presence of 1 mM ADP

712440

1.1.1.41

0.11

-

-

286749

1.1.1.41

0.12

-

pH 7.5

654843

1.1.1.41

0.143

-

pH 7.5, 37°C, recombinant enzyme mutant S102G

723607

1.1.1.41

0.148

-

pH 7.5, 37°C, recombinant enzyme mutant S102A

723607

1.1.1.41

0.15

-

pH 7.5, 37°C, recombinant enzyme mutant S102T

723607

1.1.1.41

0.28

-

pH 7.2, 25°C, recombinant mutant betaY137F, in presence of 1 mM ADP

712440

1.1.1.41

0.32

-

purified recombinant wild-type enzyme, pH 7.2, 25°C, in presence of 1 mM ADP

656189

1.1.1.41

0.385

-

pH 7.5, 37°C, recombinant enzyme mutant S102Y

723607

1.1.1.41

0.45

-

-

286749

1.1.1.41

0.5

-

pH 7.2, 25°C, recombinant wild-type enzyme, in presence of 1 mM ADP

712440

1.1.1.41

0.581

-

pH 7.4, 25°C

654783

1.1.1.41

0.77

-

purified recombinant mutant beta-R99Q, pH 7.2, 25°C, in presence of 1 mM ADP

656189

1.1.1.41

0.9

-

purified recombinant mutant beta-R99Q, pH 7.2, 25°C

656189

1.1.1.41

0.92

-

pH 7.2, 25°C, recombinant mutant betaY137S, in presence of 1 mM ADP

712440

1.1.1.41

1.2

-

pH 7.2, 25°C, recombinant mutant betaY137S

712440

1.1.1.41

1.8

-

pH 7.2, 25°C, recombinant mutant betaY137F

712440

1.1.1.41

1.8

-

purified recombinant mutant gamma-R97Q, pH 7.2, 25°C, in presence of 1 mM ADP

656189

1.1.1.41

2

-

pH 7.2, 25°C, recombinant wild-type enzyme

712440

1.1.1.41

2

-

purified recombinant mutant gamma-R97Q, pH 7.2, 25°C

656189

1.1.1.41

2.2

-

25°C, pH 7.2, wild-type enzyme

669477

1.1.1.41

2.2

-

purified recombinant wild-type enzyme, pH 7.2, 25°C

656189

1.1.1.41

2.2

-

wild type enzyme in 20 mM isocitrate and 1mM MnSO4

669477

1.1.1.41

2.4

-

pH 7.2, wild-type enzyme

685154

1.1.1.41

2.9

-

pH 7.2, beta-subunit mutant enzyme D192N

685154

1.1.1.41

3.4

-

25°C, pH 7.2, alpha-subunit mutant enzyme D234C

669477

1.1.1.41

3.4

-

D234C, alpha-subunit mutant, in 20 mM isocitrate and 1mM MnSO4

669477

1.1.1.41

3.4

-

pH 7.2, 25°C, recombinant mutant betaY137E

712440

1.1.1.41

3.5

-

25°C, pH 7.2, alpha-subunit mutant enzyme D206N

669477

1.1.1.41

3.5

-

D206N, alpha-subunit mutant, in 20 mM isocitrate and 1mM MnSO4

669477

1.1.1.41

5.1

-

25°C, pH 7.2, beta-subunit mutant enzyme D217N

669477

1.1.1.41

5.1

-

D217N, beta-subunit mutant, in 20 mM isocitrate and 1mM MnSO4

669477

1.1.1.41

9.1

-

pH 7.2, alpha-subunit mutant enzyme D181N

685154

1.1.1.41

13

-

pH 7.2, gamma-subunit mutant enzyme D190N

685154

1.1.1.41

20

-

25°C, pH 7.2, alpha-subunit mutant enzyme D230C

669477

1.1.1.41

20

-

D230C, alpha-subunit mutant, in 20 mM isocitrate and 1mM MnSO4

669477

1.1.1.41

41

-

25°C, pH 7.2, gamma-subunit mutant enzyme D215N

669477

1.1.1.41

41

-

D215N, gamma-subunit mutant, in 20 mM isocitrate and 1mM MnSO4

669477

1.1.1.42

0.00013

-

pH 7.5, 30°C

655094

1.1.1.42

0.0006

-

pH 7.4, mutant Y140T

656180

1.1.1.42

0.0007

-

pH 7.4, mutant Y140E

656180

1.1.1.42

0.001

-

pH 8.0, forward reaction

656740

1.1.1.42

0.0017

-

isozyme 2

286782

1.1.1.42

0.0022

-

-

286786

1.1.1.42

0.0026

-

-

286749

1.1.1.42

0.003

-

mutant enzyme K413Q, at pH 8.0 and 37°C

762409

1.1.1.42

0.0031

-

isozyme 1

286782

1.1.1.42

0.004

-

mitochondrial isozyme, forward reaction, pH 8.0

654843

1.1.1.42

0.0042

-

pH 7.5, isoenzyme IDP2

684709

1.1.1.42

0.005

-

wild type enzyme, at pH 8.0 and 37°C

762409

1.1.1.42

0.0052

-

pH 7.4, mutant Y140F

656180

1.1.1.42

0.0053

-

pH 7.4, mutant Y140K

656180

1.1.1.42

0.0057

-

Mn2+-dependent reaction

286803

1.1.1.42

0.0058

-

pH 7.5, isoenzyme IDP1

684709

1.1.1.42

0.0059

-

pH 7.4, recombinant mutant Y316F

654341

1.1.1.42

0.006

-

cytosolic isozyme, forward reaction, pH 8.0

654843

1.1.1.42

0.0062

-

pH 7.4, recombinant mutant K323Q

654341

1.1.1.42

0.0062

-

recombinant wild-type IDH1, pH not specified in the publication, temperature not specified in the publication

713508

1.1.1.42

0.0064

-

mutant T311S, pH 7.4, 25°C

669373

1.1.1.42

0.0065

-

mutant R83K, pH 7.4, 25°C

669373

1.1.1.42

0.0073

-

mutant T311N, pH 7.4, 25°C

669373

1.1.1.42

0.0074

-

pH 7.4, recombinant wild-type enzyme

654341

1.1.1.42

0.0075

-

pH 7.4, wild-type enzyme

656180

1.1.1.42

0.0075

-

recombinant wild-type enzyme, pH 7.4, 25°C

657309

1.1.1.42

0.008

-

at pH 6.0 and 42°C

762222

1.1.1.42

0.0082

-

recombinant enzyme, pH 7.5, 30°C

721312

1.1.1.42

0.0083

-

pH 7.4, 25°C, recombinant mutant H319Q

654649

1.1.1.42

0.0084

-

pH 7.4, 25°C, recombinant wild-type

654649

1.1.1.42

0.0084

-

wild-type, pH 7.4, 25°C

669373

1.1.1.42

0.0087

-

pH 7.4, 25°C, recombinant mutant H315Q

654649

1.1.1.42

0.009

-

pH 8.0, 55°C, cofactor: NADP+

719515

1.1.1.42

0.009

-

pH 8.0, forward reaction

656740

1.1.1.42

0.0091

-

-

286779

1.1.1.42

0.0092

-

pH 7.4, mutant K212Y

656180

1.1.1.42

0.0097

-

25°C assay temperature

286779

1.1.1.42

0.0098

-

pH 7.4, mutant K212Q

656180

1.1.1.42

0.01

-

isoform ICD-1, presence of Mg2+, pH 7.5, 25°C

668080

1.1.1.42

0.01

-

pH 7.4, recombinant mutant K321Q

654341

1.1.1.42

0.011

-

mutant R291S/K343D/Y344I/V350A/Y390P, pH 8.0, 30°C

670805

1.1.1.42

0.0114

-

mutant N328D, pH 7.4, 25°C

669373

1.1.1.42

0.0125

-

-

286779

1.1.1.42

0.0127

-

pH 9.0, 30°C

655035

1.1.1.42

0.014

-

mutant R83Q, pH 7.4, 25°C

669373

1.1.1.42

0.014

-

recombinant mutant N97A, pH 7.4, 25°C

657309

1.1.1.42

0.0147

-

pH 8.0, 28°C

722896

1.1.1.42

0.015

-

pH 7.5, isoenzyme IDP3

684709

1.1.1.42

0.015

-

wild type enzyme, at pH 7.5 and 21°C

761477

1.1.1.42

0.0154

-

mutant N328S, pH 7.4, 25°C

669373

1.1.1.42

0.0155

-

mutant T311A, pH 7.4, 25°C

669373

1.1.1.42

0.01558

-

mutant enzyme L594E, at pH 8.0 and 20°C

761559

1.1.1.42

0.017

-

enzyme from ischemic heart, pH 7.8, 25°C

670236

1.1.1.42

0.017

-

pH 7.8, 25°C, enzyme from ischemic heart

670236

1.1.1.42

0.018

-

mutant R291S, pH 8.0, 30°C

670805

1.1.1.42

0.0181

-

70°C assay temperature

286779

1.1.1.42

0.02

-

isoform ICD-2, presence of Mg2+, pH 7.5, 25°C

668080

1.1.1.42

0.02008

-

wild type enzyme, at pH 8.0 and 20°C

761559

1.1.1.42

0.0204

-

in presence of Mg2+

286807

1.1.1.42

0.021

-

pH and temperature not specified in the publication

723129

1.1.1.42

0.0217

-

mutant enzyme R153H, at pH 7.5 and 25°C

761403

1.1.1.42

0.022

-

Mn2+-dependent reaction

286799

1.1.1.42

0.022

-

isoform ICD-1, presence of Zn2+, pH 7.5, 25°C

668080

1.1.1.42

0.0242

-

wild type enzyme, at pH 7.5 and 25°C

761403

1.1.1.42

0.027

-

wild type enzyme, in potassium phosphate buffer, at pH 6.2 and 37°C

760535

1.1.1.42

0.029

-

-

680481

1.1.1.42

0.03

-

isozyme IDP1

654367

1.1.1.42

0.03

-

pH 7.5, isoenzyme IDP1

684709

1.1.1.42

0.03

-

wild type enzyme, in Tris buffer, at pH 7.5 and 37°C

760535

1.1.1.42

0.0304

-

mutant K256Q of isoform IDH2, at pH 7.4 and 25°C

762446

1.1.1.42

0.0318

-

mutant enzyme R153C, at pH 7.5 and 25°C

761403

1.1.1.42

0.0326

-

with Mg2+, pH 7.5, 37°C

723152

1.1.1.42

0.0326

-

with Mg2+, pH 7.5, 50°C

723152

1.1.1.42

0.033

-

-

286809

1.1.1.42

0.03353

-

at pH 8.0 and 20°C

761052

1.1.1.42

0.034

-

wild type enzyme, in potassium phosphate buffer, at pH 6.5 and 37°C

760535

1.1.1.42

0.035

-

wild type enzyme, in Tris buffer, at pH 7.8 and 37°C

760535

1.1.1.42

0.036

-

mutant R291S/K343D/Y390P, pH 8.0, 30°C

670805

1.1.1.42

0.036

-

mutant R291S/Y390P, pH 8.0, 30°C

670805

1.1.1.42

0.038

-

wild type enzyme, in Tris buffer, at pH 8.0 and 37°C

760535

1.1.1.42

0.04

-

mutant R291S/K343D/Y344I/Y390P, pH 8.0, 30°C

670805

1.1.1.42

0.04

-

wild type enzyme, in potassium phosphate buffer, at pH 6.8 and 37°C

760535

1.1.1.42

0.0405

-

40°C, pH 8.0

722205

1.1.1.42

0.042

-

wild type enzyme, in Bis-Tris buffer, at pH 6.2 and 37°C

760535

1.1.1.42

0.043

-

at pH 7.4 and 37°C

761866

1.1.1.42

0.045

-

enzyme from normoxic heart, pH 7.8, 25°C

670236

1.1.1.42

0.045

-

pH 7.8, 25°C, enzyme from normoxic heart

670236

1.1.1.42

0.045

-

recombinant enyme, pH 9.0

655430

1.1.1.42

0.04666

-

mutant enzyme E596L, at pH 8.0 and 20°C

761559

1.1.1.42

0.048

-

wild type enzyme, in Bis-Tris buffer, at pH 6.5 and 37°C

760535

1.1.1.42

0.05

-

isozyme IDP3

654367

1.1.1.42

0.05

-

pH 7.5, temperature not specified in the publication, recombinant His-tagged enzyme

721687

1.1.1.42

0.05

-

wild type enzyme, in potassium phosphate buffer, at pH 7.0 and 37°C

760535

1.1.1.42

0.0505

-

in the presence of Mn2+, pH and temperature not specified in the publication

760653

1.1.1.42

0.0505

-

pH 8.0, 25°C, in presence of Mn2+, wild-type enzyme

760653

1.1.1.42

0.054

-

pH and temperature not specified in the publication

723129

1.1.1.42

0.0544

-

wild type isoform IDH2, at pH 7.4 and 25°C

762446

1.1.1.42

0.0555

-

mutant K413Q of isoform IDH2, at pH 7.4 and 25°C

762446

1.1.1.42

0.059

-

Mg2+-dependent reaction

286803

1.1.1.42

0.059

-

pH 7.5, 25°C, recombinant enzyme, with Mg2+

739874

1.1.1.42

0.06

-

wild type enzyme, at pH 8.0 and 20°C

761559

1.1.1.42

0.06

-

wild type enzyme, in potassium phosphate buffer, at pH 7.2 and 37°C

760535

1.1.1.42

0.061

-

wild type enzyme, in potassium phosphate buffer, at pH 7.5 and 37°C

760535

1.1.1.42

0.0654

-

in the presence of Mg2+, pH and temperature not specified in the publication

760653

1.1.1.42

0.0654

-

pH 8.0, 25°C, in presence of Mg2+, wild-type enzyme

760653

1.1.1.42

0.066

-

recombinant mutant T78A, pH 7.4, 25°C

657309

1.1.1.42

0.066

-

wild type enzyme, in potassium phosphate buffer, at pH 7.8 and 37°C

760535

1.1.1.42

0.067

-

wild type enzyme, in potassium phosphate buffer, at pH 8.0 and 37°C

760535

1.1.1.42

0.06742

-

mutant enzyme A741S, at pH 8.0 and 20°C

761559

1.1.1.42

0.07

-

Mg2+-dependent reaction

286799

1.1.1.42

0.07

-

chloroplastic isozyme ICDH2, pH 7.8, 25°C

656616

1.1.1.42

0.072

-

-

286798

1.1.1.42

0.072

-

recombinant mutant N97D, pH 7.4, 25°C

657309

1.1.1.42

0.074

-

-

286795

1.1.1.42

0.075

-

wild type enzyme, in Bis-Tris buffer, at pH 7.0 and 37°C

760535

1.1.1.42

0.08

-

-

286805

1.1.1.42

0.095

-

mutant enzyme C3636S, at pH 7.5 and 25°C

760361

1.1.1.42

0.099

-

wild type enzyme, at pH 7.5 and 25°C

760361

1.1.1.42

0.101

-

recombinant mutant S95A, pH 7.4, 25°C

657309

1.1.1.42

0.1043

-

pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S22A

760653

1.1.1.42

0.105

-

recombinant, renatured enzyme, pH 8.0

655604

1.1.1.42

0.108

-

wild-type, pH 8.0, 30°C

670805

1.1.1.42

0.11

-

wild type enzyme, in Tris buffer, at pH 7.2 and 37°C

760535

1.1.1.42

0.116

-

pH 7.4, mutant K212R

656180

1.1.1.42

0.1169

-

pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S22H

760653

1.1.1.42

0.118

-

-

286808

1.1.1.42

0.12

-

pH 7.5, 25°C, recombinant enzyme, with Mn2+

739874

1.1.1.42

0.1243

-

with Mn2+, pH 7.5, 37°C

723152

1.1.1.42

0.1243

-

with Mn2+, pH 7.5, 50°C

723152

1.1.1.42

0.13

-

pH 8.0, 30°C, cofactor: NADP+

719515

1.1.1.42

0.1414

-

pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S22E

760653

1.1.1.42

0.15

-

mutant enzyme K217M, in potassium phosphate buffer, at pH 7.5 and 37°C

760535

1.1.1.42

0.18

-

cytosolic isozyme ICDH1, pH 7.8, 25°C

656616

1.1.1.42

0.22

-

isozyme IDP2

654367

1.1.1.42

0.22

-

pH 7.5, isoenzyme IDP2

684709

1.1.1.42

0.22

-

wild type enzyme, at pH 7.5 and 37°C

761477

1.1.1.42

0.232

-

at pH 8.2 and 18°C

761784

1.1.1.42

0.2447

-

pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S113T

760653

1.1.1.42

0.25

-

mutant enzyme K217Q, in potassium phosphate buffer, at pH 7.5 and 37°C

760535

1.1.1.42

0.28

-

mutant enzyme H133Q, at pH 7.5 and 21°C

761477

1.1.1.42

0.3276

-

pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S113A

760653

1.1.1.42

0.332

-

60°C, pH 8.0

722205

1.1.1.42

0.367

-

recombinant mutant T78D, pH 7.4, 25°C

657309

1.1.1.42

0.3686

-

recombinant mutant R132C IDH1, pH not specified in the publication, temperature not specified in the publication

713508

1.1.1.42

0.4

-

mutant enzyme H133Q, at pH 7.5 and 37°C

761477

1.1.1.42

0.4341

-

recombinant mutant R132S IDH1, pH not specified in the publication, temperature not specified in the publication

713508

1.1.1.42

0.5824

-

recombinant mutant R132H IDH1, pH not specified in the publication, temperature not specified in the publication

713508

1.1.1.42

0.8

-

mutant enzyme R132Q, at pH 7.5 and 37°C

761477

1.1.1.42

1.1

-

mutant enzyme R132K, at pH 7.5 and 37°C

761477

1.1.1.42

1.5

-

mutant enzyme R132N, at pH 7.5 and 37°C

761477

1.1.1.42

1.9

-

mutant enzyme D273L, in potassium phosphate buffer, at pH 7.5 and 37°C

760535

1.1.1.42

2.07

-

recombinant mutant S95D, pH 7.4, 25°C

657309

1.1.1.42

2.525

-

pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S113G

760653

1.1.1.42

2.7

-

mutant enzyme A134D, at pH 7.5 and 21°C

761477

1.1.1.42

3.6

-

mutant enzyme R132G, at pH 7.5 and 21°C

761477

1.1.1.42

3.6

-

mutant enzyme R132W, at pH 7.5 and 37°C

761477

1.1.1.42

4.2

-

mutant enzyme R132H, at pH 7.5 and 37°C

761477

1.1.1.42

5.3

-

mutant enzyme R132C, at pH 7.5 and 21°C

761477

1.1.1.42

5.7

-

mutant enzyme R132A, at pH 7.5 and 37°C

761477

1.1.1.42

6

-

mutant enzyme R132H, at pH 7.5 and 21°C

761477

1.1.1.42

7

-

mutant enzyme R132G, at pH 7.5 and 37°C

761477

1.1.1.42

8

-

mutant enzyme A134D, at pH 7.5 and 37°C

761477

1.1.1.42

8

-

mutant enzyme R100Q, at pH 7.5 and 37°C

761477

1.1.1.42

8.2

-

mutant enzyme R132C, at pH 7.5 and 37°C

761477

1.1.1.42

9

-

mutant enzyme R100Q, at pH 7.5 and 21°C

761477

1.1.1.42

12

-

mutant enzyme D273S, in potassium phosphate buffer, at pH 7.5 and 37°C

760535

1.1.1.42

19

-

mutant enzyme D273N, in potassium phosphate buffer, at pH 7.5 and 37°C

760535

1.1.1.87

0.014

-

wild type enzyme, with NAD+ as cosubstrate, at pH 8.0 and 60°C

760522

1.1.1.87

0.0164

-

pH 7.8, 70°C, recombinant enzyme

660907

1.1.1.87

0.161

-

pH 7.5, 60°C, recombinant mutant V135M

669109

1.1.1.87

0.291

-

pH 7.8, 25°C, recombinant wild-type enzyme

660918

1.1.1.87

0.291

-

pH 7.8, 28°C, recombinant enzyme

667934

1.1.1.87

0.3

-

mutant enzyme S80A, with NAD+ as cosubstrate, at pH 8.0 and 60°C

760522

1.1.1.87

0.4

-

purified enzyme, pH 8.0, 60°C

656127

1.1.1.87

0.465

-

pH 7.8, 25°C, recombinant mutant A80del

660918

1.1.1.87

0.5

-

mutant enzyme T71V, with NAD+ as cosubstrate, at pH 8.0 and 60°C

760522

1.1.1.87

0.521

-

pH 7.5, 60°C, recombinant wild-type enzyme

669109

1.1.1.87

0.58

-

mutant enzyme L83R, with NAD+ as cosubstrate, at pH 8.0 and 60°C

760522

1.1.1.87

1.2

-

mutant enzyme L81P, with NAD+ as cosubstrate, at pH 8.0 and 60°C

760522

1.1.1.87

2.2

-

mutant enzyme I82N, with NAD+ as cosubstrate, at pH 8.0 and 60°C

760522

2.1.1.144

5

-

-

288662

2.1.1.144

23.1

-

-

288663

2.3.1.126

0.208

-

second substrate caffeoyl-CoA

486247

2.3.1.126

0.257

-

second substrate p-coumaroyl-CoA

486247

2.3.1.126

0.412

-

second substrate feruloyl-CoA

486247

4.1.3.1

0.015

-

-

33115

4.1.3.1

0.0157

-

pH 6.5

653476

4.1.3.1

0.016

-

pH 7.5, 25°C

713812

4.1.3.1

0.018

-

pH 6.8

33116

4.1.3.1

0.032

-

phosphate, pH 6.0

33138

4.1.3.1

0.033

-

HEPES, pH 7.0

33123, 33138

4.1.3.1

0.035

-

imidazole, pH 7.0

33138

4.1.3.1

0.039

-

MOPS, pH 6.8

33138

4.1.3.1

0.039

-

pH 7, standard g, cleavage reaction

650574

4.1.3.1

0.043

-

pH 7, microgravity, cleavage reaction

650574

4.1.3.1

0.045

-

isoform ICL1, with 5 mM MgCl2, at pH 7.5 and 25°C

748480

4.1.3.1

0.045

-

pH 7.5, 25°C, recombinant enzyme

727039

4.1.3.1

0.045

-

recombinant detagged enzyme, pH 7.0, 37°C

727045

4.1.3.1

0.05

-

pH 7.5, 25°C

713812

4.1.3.1

0.056

-

ICL1

702178

4.1.3.1

0.06

-

at pH 7.4 and 25°C

748615

4.1.3.1

0.06

-

100 mM Hepes, pH 7.0

33147

4.1.3.1

0.062

-

pH 7.3

33116, 33118

4.1.3.1

0.063

-

isoform ICL2, with 5 mM MgCl2, at pH 7.5 and 25°C

748480

4.1.3.1

0.066

-

-

33136

4.1.3.1

0.07

-

-

33151

4.1.3.1

0.083

-

ICL2

702178

4.1.3.1

0.087

0.096

-

33146

4.1.3.1

0.093

-

-

33152

4.1.3.1

0.1

-

ICL

702178

4.1.3.1

0.1

0.2

Tris, triethanolamine or imidazole buffer

33130, 33131

4.1.3.1

0.11

-

TRA, pH 7.0

33138

4.1.3.1

0.136

-

-

33155

4.1.3.1

0.159

-

in 50 mM potassium phosphate, 4 mM MgCl2, 4 mM phenylhydrazine, 12 mM cysteine, pH 7.0, at 37°C

679472

4.1.3.1

0.188

-

ICL1, in 50 mM MOPS pH 6.8, 5 mM MgCl2

681995

4.1.3.1

0.25

-

-

33125

4.1.3.1

0.25

-

10°C

666268

4.1.3.1

0.25

-

MOPS, pH 7.5

33138

4.1.3.1

0.25

-

recombinant His-tagged mutant N214D, pH 7.8, 30°C

728845

4.1.3.1

0.26

-

mutant Q217K, 10°C

666268

4.1.3.1

0.29

-

phosphate, pH 7.5

33138

4.1.3.1

0.29

-

recombinant His-tagged mutant Q211H, pH 7.8, 30°C

728845

4.1.3.1

0.3

-

phosphate, pH 7.0

33138

4.1.3.1

0.33

-

wild-type, 10°C

666268

4.1.3.1

0.44

-

recombinant His-tagged wild-type enzyme, pH 7.8, 30°C

728845

4.1.3.1

0.47

-

recombinant His-tagged mutant Q221K, pH 7.8, 30°C

728845

4.1.3.1

0.47

-

wild-type, 20°C

666268

4.1.3.1

0.5

-

mutant Q217K, 30°C

666268

4.1.3.1

0.51

-

20°C, pH 6.8

638504

4.1.3.1

0.51

-

50 mM MOPS buffer, pH 7.5, with 1 mM MgCl2, 10 mM 2-mercaptoethanol, and 2.7 mM O-nitrophenyl-beta-D-galactopyranoside

679978

4.1.3.1

0.52

-

60 mM potassium phosphate, pH 7.5

33147

4.1.3.1

0.5287

-

mutant enzyme T534A, at pH 7.0 and 37°C

748505

4.1.3.1

0.5287

-

wild type enzyme, at pH 7.0 and 37°C

748505

4.1.3.1

0.5433

-

mutant enzyme T534V, at pH 7.0 and 37°C

748505

4.1.3.1

0.6

-

-

33149

4.1.3.1

0.62

-

imidazole-HCl buffer pH 6.85

33153

4.1.3.1

0.633

-

15°C, pH 6.8, Km increases with elevating temperature

651215

4.1.3.1

0.65

-

40°C

666268

4.1.3.1

0.66

-

Tris, pH 7.6

33138

4.1.3.1

0.67

-

phosphate, pH 7.5

33138

4.1.3.1

0.72

-

-

679815

4.1.3.1

0.76

-

60 mM triethanolamine, pH 7.5

33147

4.1.3.1

0.76

-

mutant Q207H, 10°C

666268

4.1.3.1

0.94

-

recombinant His-tagged mutant E219A, pH 7.8, 30°C

728845

4.1.3.1

0.96

-

pH 7.5, 70°C, recombinant enzyme

715548

4.1.3.1

1

-

phosphate buffer, pH 6.8 or 7.5

33130, 33131

4.1.3.1

1

-

phosphate, pH 6.8

33138

4.1.3.1

1.14

-

ICL2, in Tricine-HCl pH 7.5, 5 mM MgCl2

681995

4.1.3.1

1.2

1.7

-

33120

4.1.3.1

1.4

-

pH 7.5, 25°C

664147

4.1.3.1

1.532

-

sodium phosphate buffer, pH 6.85

33153

4.1.3.1

1.6

-

pH 7

649454

4.1.3.1

2.07

-

mutant Q207H, 30°C

666268

4.1.3.1

6.7

-

when acetyl-CoA is present at concentrations higher than 5 mM, the Km value for isocitrate decreased by about one-third and the Vmax value increased about 2-fold, pH not specified in the publication, temperature not specified in the publication

715749

4.1.3.1

7.6

-

pH 6.5, 30°C

658479

4.2.1.3

0.012

-

-

33788

4.2.1.3

0.034

-

liver enzyme

33784

4.2.1.3

0.045

-

-

33788

4.2.1.3

0.08

-

cytoplasmic isoenzyme

33797

4.2.1.3

0.12

-

mitochondrial enzyme

33797

4.2.1.3

0.139

-

heart enzyme

33784

4.2.1.3

0.15

-

-

33784

4.2.1.3

0.32

-

-

33784

4.2.1.3

0.37

-

pH 8.4, 75°C

651095

4.2.1.3

0.552

-

pH 8.0, 30°C

715699

4.2.1.3

0.56

-

pH 8.0, 25°C

680491

4.2.1.3

0.58

-

-

33784

4.2.1.3

0.7

-

isoenzyme II

33791

4.2.1.3

0.9

-

pH 8, 25°C, Lineweaver-Burk method

730343

4.2.1.3

1.49

-

isoform Aco1, pH 8.0, 25°C

748481

4.2.1.3

1.68

-

isoform Aco4, pH 8.0, 25°C

748481

4.2.1.3

3

-

recombinant enzyme

652036

4.2.1.3

3.33

-

isoenzyme I

33791

1.1.1.286

12 entries

1.1.1.41

46 entries

1.1.1.42

167 entries

1.1.1.87

13 entries

2.1.1.144

2 entries

2.3.1.126

3 entries

4.1.3.1

70 entries

4.2.1.3

18 entries