Ligand H2

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Basic Ligand Information

Molecular Structure
Picture of H2 (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
H2
H2
UFHFLCQGNIYNRP-UHFFFAOYSA-N
Synonyms:
deuterium, Hydrogen


Show all pahtways known for Show all BRENDA pathways known for H2

Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (23 results)

EC NUMBER
LITERATURE
REACTION DIAGRAM
PROVEN IN VIVO REACTION
ENZYME 3D STRUCTURE
show the reaction diagram
H2 + NAD+ + oxidized ferredoxin = H+ + NADH + reduced ferredoxin
-
show the reaction diagram
H2 + ferricytochrome c3 = H+ + ferrocytochrome c3
-
show the reaction diagram
5,10-methenyltetrahydromethanopterin + H2 = 5,10-methylenetetrahydromethanopterin + H+
-
show the reaction diagram
H2 + 2-(2,3-dihydropentaprenyloxy)phenazine = 2-dihydropentaprenyloxyphenazine
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show the reaction diagram
N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + H2 = coenzyme B + coenzyme M
-
show the reaction diagram
H2 + oxidized ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB = reduced ferredoxin [iron-sulfur] cluster + CoB + CoM + H+
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show the reaction diagram
selenate + H2 = selenite + H2O
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In Vivo Product in Enzyme-catalyzed Reactions (17 results)

EC NUMBER
LITERATURE
REACTION DIAGRAM
PROVEN IN VIVO REACTION
ENZYME 3D STRUCTURE
-
show the reaction diagram
NADH + H+ + reduced ferredoxin = H2 + NAD+ + oxidized ferredoxin
-
show the reaction diagram
CO + H2O = CO2 + H2
-
-
show the reaction diagram
reduced ferredoxin [iron-sulfur] cluster + CoB + CoM + H+ = H2 + oxidized ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB
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Substrate in Enzyme-catalyzed Reactions (176 results)

EC NUMBER
LITERATURE
REACTION DIAGRAM
REACTION
ENZYME 3D STRUCTURE
show the reaction diagram
H2 + NAD+ + oxidized ferredoxin = H+ + NADH + reduced ferredoxin
-
show the reaction diagram
5,10-methenyltetrahydromethanopterin + H2 = 5,10-methylenetetrahydromethanopterin + H+
-
show the reaction diagram
H2 + sulfur = H2S
-
show the reaction diagram
2-phenylbutyric acid + H2 = ?
-
show the reaction diagram
acetylene + H2 + ? = ethylene + ?
-
show the reaction diagram
H2 + reduced methyl viologen = methyl viologen
-
show the reaction diagram
N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + H2 = coenzyme B + coenzyme M
-
show the reaction diagram
selenate + H2 = selenite + H2O
-
show the reaction diagram
H2 + CO2 + CH3-H4SPt + CoASH = acetyl-CoA + H4SPt + H2O
-
show the reaction diagram
3'-phosphoadenosine 5'-phosphate + H2 = AMP + phosphate
-
show the reaction diagram
urokinase-type plasminogen activator receptor + H2 = ?
-
show the reaction diagram
fibronectin + H2 = ?
-
show the reaction diagram
reduced N,N,N',N'-tetramethyl-4-phenylenediamine + O2 + H2 = oxidized N,N,N',N'-tetramethyl-4-phenylenediamine + H2O
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Product in Enzyme-catalyzed Reactions (52 results)

EC NUMBER
LITERATURE
REACTION DIAGRAM
REACTION
ENZYME 3D STRUCTURE
-
show the reaction diagram
2 Mn2+ + 2 H+ = 2 Mn3+ + H2
-
-
show the reaction diagram
17alpha-hydroxypregnenolone + [reduced NADPH-hemoprotein reductase] + O2 = 3beta-hydroxyandrost-5-en-17-one + acetate + [oxidized NADPH-hemoprotein reductase] + H2
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-
show the reaction diagram
CO + H+ + ATP = C2H4 + C2H6 + C3H8 + H2O + H2 + phosphate
-
-
show the reaction diagram
NADPH + 3 H+ + 2 reduced ferredoxin [iron-sulfur] cluster = H2 + NADP+ + 2 oxidized ferredoxin [iron-sulfur] cluster
-
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show the reaction diagram
coenzyme B + coenzyme M + reduced ferredoxin + H+ + coenzyme F420 = coenzyme B-coenzyme M disulfide + ferredoxin + H2 + reduced coenzyme F420
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Activator in Enzyme-catalyzed Reactions (2 results)

COMMENTARY
EC NUMBER
LITERATURE
ENZYME 3D STRUCTURE

Inhibitor in Enzyme-catalyzed Reactions (10 results)

COMMENTARY
EC NUMBER
LITERATURE
ENZYME 3D STRUCTURE
H2 strongly inhibits bile acid oxidation by the enzyme
-
inhibits formation of N5,N10-methenyltetrahydromethanopterin
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inhibits hydrogen production
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competitive
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competitive inhibition of N2 binding
-
competitive inhibitor of N2, no inhibition of N3-, C2H2, CN- or H+ reduction
-
H2 competes with N2 binding and inhibits N2 reduction by the FeMo protein, but H2 does not inhibit NO2- reduction for the wild-type or either of the two MoFe protein variants
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CO-reduction by V-nitrogenase is inhibited by the addition of increasing amounts of H2
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inhibits both N2 and diazene reduction
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Enzyme Kinetic Parameters

kcat Value (Turnover Number) (11 results)

COMMENTARY
EC NUMBER
LITERATURE
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
-
212
-
pH 8.0, HoxI deletion mutant enzyme, with ferrocyanide
284
-
pH 8.0, HoxI deletion mutant enzyme, with NAD+
109
-
pH 8.0, HoxI deletion mutant enzyme, with oxidized benzyl viologen
70
-
pH 8.0, wild-type enzyme complex including HoxI, with ferrocyanide
372
-
pH 8.0, wild-type enzyme complex including HoxI, with NAD+
143
-
pH 8.0, wild-type enzyme complex including HoxI, with oxidized benzyl viologen
120
-
-
0.683
-
using ferredoxin CAC0587 as electron carrier, at 35°C
133
-
using flavodoxin CAC0587 as electron carrier, at 35°C
483
-
using methyl viologen as electron carrier, at 35°C
454
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KM Value (39 results)

COMMENTARY
EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
LITERATURE
cosubstrate: methyl viologen
0.02
-
cosubstrate: benzyl viologen
0.018
-
apparent Km value for H2 is about 6% in the gas phase, pH 7.5, 45°C
-999
-
using methyl viologen as electron carrier, at 35°C
0.016
-
using flavodoxin CAC0587 as electron carrier, at 35°C
0.000088
-
using ferredoxin CAC0587 as electron carrier, at 35°C
0.000033
-
+ coenzyme F0, pH 7.2
0.01
-
+ methyl viologen, pH 9
0.002
-
37°C, pH 7.2, cosubstrate: oxidized coenzyme F420
0.003
-
37°C, pH 7.2, cosubstrate: oxidized methyl viologen
0.004
-
-
0.15
-
the apparent Km for H2 is estimated to be approximately 60% H2 in the gas phase
-999
-
apparent Km for H2 is 60%
-999
-
with methyl viologen as electron acceptor
with methylene blue as electron acceptor
0.08
-
at pH 6.5, 30°C
0.01
-
at pH 6.0, 30°C
0.015
-
at pH 5.5, 40°C
0.024
-
at pH 5.5, 30°C
0.006
-
at pH 5.5, 20°C
0.001
-
at pH 5.5, 10°C
0.0004
-
at pH 5.0, 30°C
0.008
-
at pH 4.5, 30°C
0.006
-
apparent value, pH and temperature not specified in the publication
0.000047
-
pH 7, 65°C, H2: heterodisulfide oxidoreductase complex
0.03
-

References & Links

Links to other databases for H2

ChEBI
PubChem
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PubChem