Ligand nitroxyl

NO stimulates non-enzymatic ADP-ribosylation, at cysteine residues in the presence of reductant, of NUDT5 using ADP-ribose and consequently activates its ADPRase activity. ADPRase activity in J774 macrophage cells is increased by the treatment with SNP, an exogenous NO generator, or TNF-alpha/IFN-gamma, endogenous NO inducers. NO has a regulatory role, overview

684834

4.2.1.105

stimulation of expression

activation at moderate concentration, inhibition at high concentrations. Hemoglobin prevents both, activation and inhibition

37485

6.3.2.2

induces expression of heavy and light subunit via direct exposure or interleukin-1 induced

651393

7.1.1.9

2 entries

Inhibitor in Enzyme-catalyzed Reactions (96 results)

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1.1.1.1

Cys residues contained within the zinc/thiolate active center may be primary sites of NO interaction

285657

1.1.1.189

inhibition e.g. by NOC-18, a NO donor

670733

1.1.1.21

from NO donors S-nitroso-N-acetylpenicillamine or S-nitrosoglutathione in vivo and in vitro, leads to increased S-glutathiolation of the enzyme

generated from 1-(N,N-diethylamino)diazen-1-ium-1,2-diolate, competitive inhibitor, nitrosylated catalase is kinetically labile, NO tends to dissociate rapidly from the active site, binding structure, overview. Kinetic analysis of dissociation of NO from the enzyme-inhibitor complex

high concentrations, competition with O2, augmented by miconazole

feedback inhibition

overproduced NO in liver causes the suppression of FMO3 activity directly via reversible S-nitrosylation. Overproduced NO may be responsible, at least in part, for the impairment of the detoxification or metabolism by FMOs of xenobiotics, which include a number of therapeutic drugs

659829

1.14.14.154

in Huh7 human hepatoma cells, treatment with nitric oxide donors causes rapid post-translational down-regulation of the enzyme

744274

1.14.15.29

reversible inhibition

717241

1.17.1.4

dose-dependent inhibition of xanthine dehydrogenase and oxidase activity, reaction with an essential sulfur in the molybdenum center, that damages the molybdopterin

irreversible inhibitor

wild-type cultures are more sensitive to the addition of a pulse of NO when grown under fermentative conditions compared with anaerobic respiratory conditions, sensitivity of different wild-type and mutant strains, overview

strong competitive inhibitor

738690

1.7.5.2

substrate inhibition at high concentratins

inhibits GRK2 by S-nitrosylation at Cys340, regulatory function of S-nitrosylation

672951

2.7.11.17

a reduction of CaMKII activity by S-nitrosylation at Cys6 is observed, but only after prolonged exposure of over 5 min to NO donors

740722

2.7.7.64

inactivation, reactivation by reduction using cysteine or thioredoxin

737765

3.1.3.48

inactivation, phosphate protects

inhibits adenovirus replication by targeting the adenovirus proteinase, inhibition is reversible with dithiothreitol

reversible inhibition under dioxygen-depleted conditions with 100fold reduction of activity in the presence of 0.05 mM nitric oxide. At 12 min, air is re-introduced, resulting in the restoration of full OxDC catalytic activity, albeit after a time lag of approximately 5 min

714726

4.1.1.29

fusion protein with beta-galactosidase

656303

4.2.1.112

complete inhibition at 3 mM

brief exposure leads to a reversible inhibition competitive with isocitrate. subsequently, an irreversible inactivation is observed

activation at moderate concentration, reversible inhibition at high concentrations. Hemoglobin prevents both, activation and inhibition

37485

5.3.99.5

irreversible in a concentration-dependent manner

37485

5.6.2.2

NO, unlike VP-16, preferentially induces the formation of TOP2beta cleavable complexes in cells, induces skin melanomas formation in 7,12-dimethyl-benz[a]anthracene-initiated mice

mediates reversible S-nitrosylation and inactivation of argininosuccinate synthetase in vitro and in lipopolysaccharide-treated cells and mice. S-nitrosylation of Cys132 is noth necessary and sufficient for the inhibition of argininosuccinate synthase by NO donors

662254

7.1.1.3

reversible inhibition

741957

7.1.1.7

reversible inhibition

752197

7.1.1.9

12 entries

Metals and Ions (2 results)

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1.11.1.21

complexed with the enzyme, structure determination, binding kinetics

654769

1.13.11.15

NO binding to a non-heme enzyme containing manganese allows examination of the factors governing the formation and detection of the MIII-O2.- species in all forms of th enzyme. NO, and presumably O2, binding is sensitive to both the nature of the catecholic substrate present and the nature of the active-site amino acid residue at position 200, spectral analysis, overview

725557