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5 sulfur + 2 O2 + 2 H2O + 4 H+ = sulfite + thiosulfate + 2 sulfide
-
bacillithiol persulfide + O2 = bacillithiol + sulfite
-
CoASSH + O2 = CoA + sulfite
-
S-sulfanyl-L-cysteine + O2 = L-cysteine + sulfite
-
S-sulfanylglutathione + O2 + H2O = glutathione + sulfite + 2 H+
-
S-sulfanylglutathione + O2 = glutathione + sulfite
-
S-sulfanylglutathione + O2 = sulfite + glutathione + H+
-
sulfur + O2 + H2O = sulfite
-
3-sulfocatechol + O2 + H2O = (2E,4Z)-2-hydroxymuconate + bisulfite + H+
-
4 sulfur + 4 H2O + O2 = 2 hydrogen sulfide + 2 bisulfite + 2 H+
-
4 sulfur + 4 H2O + O2 = 2 hydrogen sulfide + 2 HSO3- + 2 H+
-
4 sulfur + 4 H2O + O2 = 2 hydrogen sulfide + 2 sulfite
-
3S + 3H2O = HSO3- + 2HS- + 3H+
-
S + O2 + H2O = HSO3- + H2S + H+
-
S + OH- + O2 = HSO3- + S2O32- + HS- + H+
-
S + O2 = SO32- + S2O32- + H2S
-
1,3-dioxo-2-isoindolineethanesulfonic acid + 2-oxoglutarate + O2 = sulfite + ? + succinate + CO2
-
2-oxoglutarate + 2-methylaminoethane-1-sulfonic acid + O2 = methylaminoacetaldehyde + succinate + sulfite + CO2
-
butanesulfonic acid + 2-oxoglutarate + O2 = sulfite + butanal + succinate + CO2
-
hexanesulfonic acid + 2-oxoglutarate + O2 = sulfite + hexanal + succinate + CO2
-
hexyl sulfate + 2-oxoglutarate + O2 = hexanal + sulfite + succinate + CO2
-
MOPS + 2-oxoglutarate + O2 = sulfite + ? + succinate + CO2
-
N-methyltaurine + 2-oxoglutarate + O2 = CO2 + succinate + sulfite + methylaminoacetaldehyde
-
pentanesulfonic acid + 2-oxoglutarate + O2 = sulfite + pentanal + succinate + CO2
-
taurine + 2-oxoglutarate + O2 = CO2 + succinate + sulfite + aminoacetaldehyde
-
taurine + 2-oxoglutarate + O2 = succinate + CO2 + 2-aminoethanal + sulfite
-
taurine + 2-oxoglutarate + O2 = sulfite + aminoacetaldehyde + succinate + CO2
-
taurine + alpha-ketoadipate + O2 = sulfite + aminoacetaldehyde + pentan-1,5-dioic acid + CO2
-
4-sulfobenzoate + NADH + O2 = 3,4-dihydroxybenzoate + sulfite + NAD+
-
CH3SO3- + O2 + NADH + H+ = formaldehyde + HSO3- + NAD+ + H2O
-
methanesulfonate + NADH + H+ + O2 = formaldehyde + NAD+ + sulfite + H2O
-
6-sulfo-D-quinovose + FMNH2 + O2 = 6-dehydro-D-glucose + FMN + sulfite + H2O
-
piperazine-N,N'-bis(2-ethanesulfonic acid) + 2 FMNH2 + 2 O2 = 2,2'-piperazine-1,4-diyldiacetaldehyde + 2 FMN + 2 sulfite + 2 H2O
-
ethanesulfonate + FMNH2 + O2 = acetaldehyde + FMN + sulfite + H2O
-
isethionate + FMNH2 + O2 = hydroxyacetaldehyde + FMN + sulfite + H2O
-
methanesulfonate + FMNH2 + O2 = formaldehyde + FMN + sulfite + H2O
-
morpholinepropanesulfonic acid + FMNH2 + O2 = 3-(morpholin-4-yl)propanal + FMN + sulfite + H2O
-
pentanesulfonate + FMNH2 + O2 = pentanal + FMN + sulfite + H2O
-
1,3-dioxo-2-isoindolineethanesulfonic acid + FMNH2 + O2 = (1,3-dioxo-1,3-dihydro-2H-isoindol-2-yl)acetaldehyd + FMN + sulfite + H2O
-
2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonate + FMNH2 + O2 = ? + FMN + sulfite + H2O
-
3-(N-morpholino)propanesulfonate + FMNH2 + O2 = ? + FMN + sulfite + H2O
-
4-phenyl-1-butanesulfonic acid + FMNH2 + O2 = 4-phenylbutanol + FMN + sulfite + H2O
-
an alkanesulfonate + FMNH2 + O2 = an aldehyde + FMN + sulfite + H2O
-
an alkansulfonate + FMNH2 + O2 = an aldehyde + FMN + sulfite + H2O
-
butanesulfonic acid + FMNH2 + O2 = butanal + FMN + sulfite + H2O
-
decanesulfonic acid + FMNH2 + O2 = decanal + FMN + sulfite + H2O
-
hexadecanesulfonate + FMNH2 + O2 = hexadecanal + FMN + sulfite + H2O
-
hexanesulfonic acid + FMNH2 + O2 = hexanal + FMN + sulfite + H2O
-
methanesulfonate + FMNH2 + O2 = formaldehyde + FMN + sulfite + H2O
-
N-phenyltaurine + FMNH2 + O2 = anilinoacetaldehyde + FMN + sulfite + H2O
-
octanesulfonate + FMNH2 + O2 = octaldehyde + FMN + sulfite + H2O
-
octanesulfonate + FMNH2 + O2 = octanal + FMN + sulfite + H2O
-
octanesulfonic acid + FMNH2 + O2 = octanal + FMN + sulfite + H2O
-
pentanesulfonate + FMNH2 + O2 = pentaldehyde + FMN + sulfite + H2O
-
pentanesulfonic acid + FMNH2 + O2 = pentaldehyde + FMN + sulfite + H2O
-
piperazine-N,N'-bis(2-ethanesulfonate) + FMNH2 + O2 = ? + FMN + sulfite + H2O
-
R-CH2-SO3H + FMNH2 + O2 = R-CHO + FMN + sulfite + H2O
-
hydrogen sulfide + 3 NADP+ + 3 H2O = sulfite + 3 NADPH + 3 H+
hydrogen sulfide + 3 NADP+ + 3 H2O = sulfite + 3 NADPH + 3 H+
hydrogen sulfide + NADP+ + H2O = sulfite + NADPH + H+
hydrogen sulfide + NADP+ + H2O = sulfite + NADPH + H+
thiosulfate + 2 ferrocytochrome c3 = sulfite + hydrogen sulfide + 2 ferricytochrome c3
thiosulfate + 2 ferrocytochrome c3 = sulfite + hydrogen sulfide + 2 ferricytochrome c3
thiosulfate + 2 ferrocytochrome c3 = sulfite + hydrogen sulfide + 2 ferricytochrome c3
thiosulfate + reduced methyl viologen = sulfite + hydrogen sulfide + oxidized methyl viologen
thiosulfate + reduced methyl viologen = sulfite + hydrogen sulfide + oxidized methyl viologen
thiosulfate + reduced methyl viologen = sulfite + hydrogen sulfide + oxidized methyl viologen
thiosulfate + 2 ferrocytochrome c3 = sulfite + hydrogen sulfide + 2 ferricytochrome c3
-
thiosulfate + reduced benzyl viologen = sulfite + hydrogen sulfide + oxidized benzyl viologen
-
thiosulfate + reduced methyl viologen = sulfite + hydrogen sulfide + oxidized methyl viologen
-
5'-adenylyl sulfate + glutathione = AMP + sulfite + glutathione disulfide
-
5'-adenylyl sulfate + reduced dithiothreitol = AMP + sulfite + oxidized dithiothreitol
-
5'-adenylyl sulfate + thioredoxin = AMP + sulfite + thioredoxin disulfide
-
adenosine 5'-phosphosulfate + thioredoxin = AMP + sulfite + thioredoxin disulfide
-
adenylyl sulfate + thioredoxin = AMP + sulfite + thioredoxin disulfide
-
3'-adenylyl-sulfate + thioredoxin = adenosine 3',5'-bisphosphate + thioredoxin disulfide + sulfite
-
3'-phosphoadenylyl sulfate + glutaredoxin 1 = adenosine 3',5'-bisphosphate + sulfite + glutaredoxin 1 disulfide
-
3'-phosphoadenylyl sulfate + glutaredoxin Grx = adenosine 3',5'-bisphosphate + sulfite + glutaredoxin Grx disulfide
-
3'-phosphoadenylyl sulfate + glutaredoxin Grx1 = adenosine 3',5'-bisphosphate + sulfite + glutaredoxin Grx1 disulfide
-
3'-phosphoadenylyl sulfate + glutaredoxin mutant 1C14S = adenosine 3',5'-bisphosphate + sulfite + glutaredoxin mutant 1C14S disulfide
-
3'-phosphoadenylyl sulfate + thioredoxin 1 = adenosine 3',5'-bisphosphate + sulfite + thioredoxin 1 disulfide
-
3'-phosphoadenylyl sulfate + thioredoxin 2 = adenosine 3',5'-bisphosphate + sulfite + thioredoxin 2 disulfide
-
3'-phosphoadenylyl sulfate + thioredoxin = adenosine 3',5'-bisphosphate + sulfite + thioredoxin disulfide
-
3'-phosphoadenylyl sulfate + thioredoxin hTrx1 = adenosine 3',5'-bisphosphate + sulfite + thioredoxin hTrx1 disulfide
-
3'-phosphoadenylyl sulfate + thioredoxin Trx1 = adenosine 3',5'-bisphosphate + sulfite + thioredoxin Trx1 disulfide
-
3'-phosphoadenylyl sulfate + thioredoxin Trx2 = adenosine 3',5'-bisphosphate + sulfite + thioredoxin Trx2 disulfide
-
3'-phosphoadenylyl sulfate + thioredoxin TrxH1 = adenosine 3',5'-bisphosphate + sulfite + thioredoxin TrxH1 disulfide
-
3'-phosphoadenylyl sulfate + thioredoxin TrxH2 = adenosine 3',5'-bisphosphate + sulfite + thioredoxin TrxH2 disulfide
-
3'-phosphoadenylyl sulfate + thioredoxin TrxH3 = adenosine 3',5'-bisphosphate + sulfite + thioredoxin TrxH3 disulfide
-
3'-phosphoadenylyl sulfate + thioredoxin TrxH4 = adenosine 3',5'-bisphosphate + sulfite + thioredoxin TrxH4 disulfide
-
3'-phosphoadenylyl-sulfate + glutaredoxin = adenosine 3',5'-bisphosphate + glutaredoxin disulfide + sulfite
-
3'-phosphoadenylyl-sulfate + thioredoxin = adenosine 3',5'-bisphosphate + thioredoxin disulfide + sulfite
-
5-Phosphoadenosine 3-phosphosulfate + reduced thioredoxin = adenosine-3',5'-bisphosphate + oxidized thioredoxin + sulfite
-
adenosine 5'-phosphosulfate + thioredoxin = AMP + sulfite + oxidized thioredoxin
-
adenosine 5'-phosphosulfate + thioredoxin I = AMP + sulfite + oxidized thioredoxin I
-
3'-phosphoadenosine 5'-phosphosulfate + glutathione = adenosine 3',5'-diphosphate + sulfite + oxidized glutathione
-
3'-phosphoadenosine-5'-phosphosulfate + glutathione = adenosine 3',5'-bisphosphate + sulfite + oxidized glutathione
-
3'-phosphoadenosine-5'-phosphosulfate + glutathione = adenosine 3',5'-diphosphate + sulfite + oxidized glutathione
-
5'-adenylyl sulfate + glutathione = AMP + sulfite + glutathione disulfide
-
5'-adenylyl sulfate + thioredoxin = AMP + sulfite + thioredoxin disulfide
-
5'-adenylylsulfate + dithioerythritol = adenosine 5'-monophosphate + sulfite + oxidized dithioerythritol
-
5'-adenylylsulfate + dithiothreitol = adenosine 5'-monophosphate + sulfite + oxidized dithiothreitol
-
5'-adenylylsulfate + glutathione + glutathione = adenosine monophosphate + sulfite + oxidized glutathione
-
5'-adenylylsulfate + glutathione = adenosine monophosphate + sulfite + oxidized glutathione
-
adenylyl sulfate + 2 glutathione = AMP + sulfite + glutathione disulfide
-
adenylyl sulfate + glutathione = AMP + sulfite + glutathione disulfide
-
thiosulfate + menaquinol = sulfite + hydrogen sulfide + menaquinone
-
hydrogen sulfide + 6 oxidized ferredoxin I [iron-sulfur] cluster + 3 H2O = sulfite + 6 reduced ferredoxin I [iron-sulfur] cluster + 6 H+
-
hydrogen sulfide + 6 oxidized ferredoxin [iron-sulfur] cluster + 3 H2O = sulfite + 6 reduced ferredoxin [iron-sulfur] cluster + 6 H+
-
hydrogen sulfide + 6 oxidized methyl viologen [iron-sulfur] cluster + 3 H2O = sulfite + 6 reduced methyl viologen [iron-sulfur] cluster + 6 H+
-
hydrogen sulfide + oxidized ferredoxin + H2O = sulfite + reduced ferredoxin + H+
-
hydrogen sulfide + oxidized methyl viologen + H2O = sulfite + reduced methyl viologen + H+
-
5'-adenylylsulfate + dithioerythritol = adenosine 5'-monophosphate + sulfite + oxidized dithioerythritol
5'-adenylylsulfate + dithiothreitol = adenosine 5'-monophosphate + sulfite + oxidized dithiothreitol
adenylyl sulfate + cofactor sulfide = AMP + sulfite + cofactor disulfide
adenylyl sulfate + dithioerythritol = AMP + sulfite + ?
adenylyl sulfate + dithioerythritol = AMP + sulfite + oxidized dithioerythritol
adenylyl sulfate + dithionite = AMP + sulfite + ?
adenylyl sulfate + dithiothreitol = AMP + sulfite + ?
adenylyl sulfate + ferrocytochrome c3 = AMP + sulfite + ferricytochrome
adenylyl sulfate + GSH = AMP + sulfite + GSSG
adenylyl sulfate + reduced acceptor = AMP + sulfite + acceptor
adenylyl sulfate + reduced glutaredoxin 1 = AMP + sulfite + oxidized glutaredoxin 1
adenylyl sulfate + reduced methyl viologen = AMP + sulfite + methyl viologen
adenylyl sulfate + reduced riboflavin = AMP + sulfite + oxidized riboflavin
adenylyl sulfate + reduced thioredoxin 1 = AMP + sulfite + oxidized thioredoxin 1
adenylyl sulfate + reduced thioredoxin = AMP + sulfite + oxidized thioredoxin
adenylylsulfate + thioredoxin = AMP + sulfite + ?
guanylyl sulfate + reduced methyl viologen = GMP + methyl viologen + sulfite
hydrogen sulfide + a [DsrC protein]-disulfide + acceptor + H2O = sulfite + a [DsrC protein]-dithiol + reduced acceptor + H+
-
2-sulfoacetaldehyde + phosphate = acetyl phosphate + sulfite
-
4-aminobutyrate + 2-oxoglutarate = L-glutamate + acetaldehyde + sulfite
-
4-(dimethylamino)-4'-azobenzene sulfinate + thiosulfate = 4-(dimethylamino)-4'-azobenzene thiosulfonate + SO32-
5-dimethylamino-1-naphthalene sulfinate + thiosulfate = 5-dimethylamino-1-naphthalene thiosulfonate + SO32-
thiosulfate + 2 glutathione = sulfite + glutathione disulfide + sulfide
thiosulfate + cyanide = sulfite + thiocyanate
thiosulfate + glutathione = sulfite + glutathione disulfide
thiosulfate + sulfite = sulfite + thiosulfate
thiosulfate + cyanide = SO32- + thiocyanate
thiosulfate + cyanide = SO32- + thiocyanate
thiosulfate + cyanide = sulfite + thiocyanate
thiosulfate + cyanide = sulfite + thiocyanate
thiosulfate + glutathione = sulfite + glutathione disulfide
thiosulfate + glutathione = sulfite + glutathione disulfide
thiosulfate + cyanide = sulfite + thiocyanate
-
thiosulfate + glutathione = sulfite + glutathione disulfide + sulfide
-
thiosulfate + 2 dithioerythritol = sulfite + dithioerythritol disulfide + sulfide
-
thiosulfate + 2 dithiothreitol = sulfite + dithiothreitol disulfide + sulfide
-
thiosulfate + dithiothreitol = sulfite + oxidized dithiothreitol + sulfide
-
thiosulfate + menaquinol = sulfite + oxidized menaquinol + sulfide
-
thiosulfate + NAD(P)H = H2S + NAD(P)+ + sulfite
-
thiosulfate + NADH = H2S + NAD+ + sulfite
-
L-cysteine sulfinic acid = L-alanine + sulfite
-
adenosine 5'-phosphosulfate + glutathione = SO32- + ?
-
adenosine 5'-phosphosulfate + dithioerythritol = sulfite + ?
-
adenosine 5'-phosphosulfate + dithiothreitol = sulfite + ?
-
4-sulfomuconolactone + H2O = maleylacetate + sulfite
-
UDP-alpha-D-sulfoquinovopyranose + H2O = UDP-alpha-D-glucose + sulfite
-
2'-hydroxybiphenyl-2-sulfinate + H2O = 2-hydroxybiphenyl + sulfite
-
2-(2'-hydroxyphenyl)benzenesulfinate + H2O = 2-hydroxybiphenyl + sulfite
-
2-(2'-hydroxyphenyl)ethene-1-sulfinate + H2O = o-hydroxystyrene + sulfite
-
2-(2-hydroxyphenyl)benzenesulfinate + H2O = 2-hydroxybiphenyl + sulfite
-
2-phenylbenzenesulfinate + H2O = biphenyl + sulfite
-
3-sulfinopropanoyl-CoA + H2O = propanoyl-CoA + sulfite
-
3-sulfinopropionyl-CoA + H2O = propionyl-CoA + sulfite
-
(2R)-3-sulfolactate = pyruvate + bisulfite
-
3-sulfolactate = pyruvate + bisulfite
-
L-cysteate + H2O = pyruvate + bisulfite + NH3
-
(S)-cysteate + H2O = HSO3- + pyruvate + NH3
-
2-hydroxyethane-1-sulfonate = acetaldehyde + H+ + sulfite
-
isethionate = acetaldehyde + H+ + sulfite
-
isethionate = acetaldehyde + sulfite
-
(2S)-2,3-dihydroxypropane-1-sulfonate = hydroxyacetone + sulfite
-
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0.023
-
modified enzyme that contains an additional covalent bond between residues Tyr303 and Gln360, pH 7.0, 20°C
0.039
-
unmodified enzyme, pH 7.0, 20°C
65
-
cosubstrate reduced methyl viologen
0.4
-
mutant enzyme Y236F, in 20 mM Tris-acetate buffer pH 8.0
0.8
-
mutant enzyme Y236F, in 20 mM bis-Tris-acetate buffer pH 6.0
2
3.7
25°C, 20 mM Tris acetate buffer, pH 10.0, wild-type enzyme
10.6
-
tether deletion mutant enzyme DELTAKVATV, in 20 mM Tris acetate, pH 8.0, at 25°C
26.9
-
wild type enzyme, in 20 mM Tris acetate, pH 8.0, at 25°C
33.51
-
wild type enzyme, in 20 mM bis-Tris-acetate buffer pH 6.0
35
-
tether deletion mutant enzyme DELTAKVAT, in 20 mM Tris acetate, pH 8.0, at 25°C
36.19
-
mutant enzyme Y236F, in 20 mM bis-Tris-acetate buffer pH 6.0
36.8
-
25°C, 20 mM Tris acetate buffer, pH 6.0, mutant Y236F
39
-
mutant enzyme P105A, in 20 mM Tris acetate, pH 8.0, at 25°C
40
-
tether deletion mutant enzyme DELTAKVA, in 20 mM Tris acetate, pH 8.0, at 25°C
41.6
-
25°C, 20 mM Tris acetate buffer, pH 6.5, mutant Y236F
46
-
mutant enzyme P105A/P111A, in 20 mM Tris acetate, pH 8.0, at 25°C
48.3
-
25°C, 20 mM Tris acetate buffer, pH 7.5, mutant Y236F
50
-
mutant enzyme P111A, in 20 mM Tris acetate, pH 8.0, at 25°C
51.8
-
25°C, 20 mM Tris acetate buffer, pH 7.0, mutant Y236F
52.51
-
mutant enzyme Y236F, in 20 mM Tris-acetate buffer pH 8.0
52.7
-
25°C, 20 mM Tris acetate buffer, pH 9.5, mutant Y236F
53.4
-
25°C, 20 mM Tris acetate buffer, pH 8.0, mutant Y236F
58.3
-
25°C, 20 mM Tris acetate buffer, pH 8.5, mutant Y236F
63.5
-
25°C, 20 mM Tris acetate buffer, pH 6.0, wild-type enzyme
64
-
25°C, 20 mM Tris acetate buffer, pH 6.2, mutant R55M
64
-
25°C, 20 mM Tris acetate buffer, pH 6.6, mutant R55M
64
-
25°C, 20 mM Tris acetate buffer, pH 9.0, mutant Y236F
66.3
-
25°C, 20 mM Tris acetate buffer, pH 7.0, mutant R55M
68.3
-
25°C, 20 mM Tris acetate buffer, pH 7.5, mutant R55M
70.5
-
25°C, 20 mM Tris acetate buffer, pH 5.8, mutant R55M
73.4
-
25°C, 20 mM Tris acetate buffer, pH 7.9, mutant R55M
86.2
-
25°C, 20 mM Tris acetate buffer, pH 6.5, wild-type enzyme
88.8
-
25°C, 20 mM Tris acetate buffer, pH 10.0, mutant Y236F
148.4
-
25°C, 20 mM Tris acetate buffer, pH 9.5, mutant H57A
158.8
-
25°C, 20 mM Tris acetate buffer, pH 7.0, wild-type enzyme
192.5
-
25°C, 20 mM Tris acetate buffer, pH 9.0, mutant H57A
214.5
-
25°C, 20 mM Tris acetate buffer, pH 7.0, mutant H57A
214.6
-
25°C, 20 mM Tris acetate buffer, pH 8.0, mutant H57A
220.5
-
25°C, 20 mM Tris acetate buffer, pH 7.5, mutant H57A
222.4
-
25°C, 20 mM Tris acetate buffer, pH 8.5, mutant H57A
226.5
-
25°C, 20 mM Tris acetate buffer, pH 6.5, mutant H57A
238.8
-
25°C, 20 mM Tris acetate buffer, pH 6.0, mutant H57A
293.4
-
25°C, 20 mM Tris acetate buffer, pH 7.5, wild-type enzyme
333.7
-
wild type enzyme, in 20 mM Tris-acetate buffer pH 8.0
345.3
-
25°C, 20 mM Tris acetate buffer, pH 8.0, wild-type enzyme
410
-
25°C, 20 mM Tris acetate buffer, pH 8.5, wild-type enzyme
431
-
25°C, 20 mM Tris acetate buffer, pH 9.5, wild-type enzyme
519
-
25°C, 20 mM Tris acetate buffer, pH 9.0, wild-type enzyme
10880
-
recombinant enzyme
0.14
-
mutant enzyme G473D in 20 mM Tris at pH 6.0
0.14
-
mutant enzyme G473D, pH 6.0
0.15
-
mutant enzyme A208D in 20 mM Tris at pH 6.0
0.28
-
mutant enzyme G473D, pH 6.5
0.42
-
mutant enzyme G473D, pH 9.1
0.46
-
mutant R472M, 25°C, pH 10.0
0.5
-
mutant enzyme G473D, pH 7.0
0.52
-
mutant R472Q, 25°C, pH 10.0
0.54
-
mutant enzyme G473D in 20 mM Tris at pH 8.5
0.54
-
mutant enzyme G473D, pH 8.5
0.57
-
mutant enzyme G473D, pH 7.5
0.58
-
mutant enzyme G473D, pH 8.0
0.6
-
mutant enzyme G473W in 20 mM Tris at pH 6.0
0.6
-
mutant enzyme G473W, pH 6.0
0.75
-
mutant enzyme A208D in 20 mM Tris at pH 8.5
0.97
-
mutant Y343N/R472Q, 25°C, pH 9.5
1.13
-
mutant Y343N/R472Q, 25°C, pH 7.0
1.13
-
mutant Y343N/R472Q, 25°C, pH 9.0
1.33
-
mutant Y343N/R472Q, 25°C, pH 8.0
1.35
-
mutant enzyme G473W, pH 9.0
1.35
-
mutant Y343N/R472Q, 25°C, pH 6.0
1.42
-
mutant Y343N/R472Q, 25°C, pH 8.5
1.44
-
mutant Y343F/R472Q, 25°C, pH 6.0
1.7
-
mutant R472Q, 25°C, pH 9.5
1.73
-
mutant Y343F/R472Q, 25°C, pH 7.0
1.8
-
mutant enzyme G473W, pH 7.0
1.8
-
mutant R472M, 25°C, pH 9.5
1.9
-
mutant Y343N/R472M/V474M, 25°C, pH 6.0
2.05
-
mutant Y343F/R472Q, 25°C, pH 8.0
2.06
-
25°C, pH 7.0, 100 mM buffer, mutant enzyme Y343F
2.25
-
mutant Y343F/R472Q, 25°C, pH 8.5
2.3
-
mutant Y343F/R472Q, 25°C, pH 9.0
2.48
-
mutant enzyme G473W in 20 mM Tris at pH 8.5
2.48
-
mutant enzyme G473W, pH 8.5
2.52
-
mutant Y322N/R450M, 25°C, pH 8.5
2.8
-
mutant Y343N/R472M/V474M, 25°C, pH 10.0
2.96
-
mutant Y343F/R472Q, 25°C, pH 10.0
3
6
mutant Y83F, pH 8.0, 25°C
3.11
-
25°C, pH 6.0, 20 mM buffer, mutant enzyme Y343F
3.17
-
mutant Y343N, 25°C, pH 6.0
3.26
-
25°C, pH 7.5, 100 mM buffer, mutant enzyme Y343F
3.4
-
mutant Y343N/R472M/V474M, 25°C, pH 7.0
3.45
-
mutant R472M, 25°C, pH 9.0
3.5
-
mutant R472M, 25°C, pH 8.0
3.58
-
mutant Y343N/R472M/V474M, 25°C, pH 8.0
3.6
-
mutant R472M, 25°C, pH 7.0
3.8
-
mutant R472M, 25°C, pH 8.5
3.9
-
mutant Y343N/R472M/V474M, 25°C, pH 8.5
4.14
-
25°C, pH 6.5, 20 mM buffer, mutant enzyme Y343F
4.15
-
mutant enzyme G473A in 20 mM Tris at pH 6.0
4.15
-
mutant enzyme G473A, pH 6.0
4.24
-
mutant R472Q, 25°C, pH 9.0
4.38
-
mutant Y343N, 25°C, pH 10.0
4.59
-
25°C, pH 7.0, 20 mM buffer, mutant enzyme Y343F
4.6
-
mutant R472Q, 25°C, pH 8.5
4.83
-
mutant Y322N/R450M, 25°C, pH 7.0
4.9
-
mutant Y343F/R472Q, 25°C, pH 9.5
5
-
mutant R472M, 25°C, pH 6.0
5.23
-
mutant Y343N/R472M/V474M, 25°C, pH 9.5
5.6
-
mutant Y343N/R472M/V474M, 25°C, pH 9.0
5.7
-
mutant R472D, pH 6.5, 25°C
5.8
-
mutant R472Q, 25°C, pH 7.0
5.96
-
mutant V474M, 25°C, pH 6.0
6.35
-
25°C, pH 7.5, 20 mM buffer, mutant enzyme Y343F
7
-
mutant Y83A, pH 8.0, 25°C
7.17
-
25°C, pH 8.0, 100 mM buffer, mutant enzyme Y343F
8.1
-
mutant Y343N, 25°C, pH 9.5
8.2
-
mutant R472Q, 25°C, pH 6.0
8.21
-
25°C, pH 10.0, 100 mM buffer, mutant enzyme Y343F
8.51
-
25°C, pH 10.0, 20 mM buffer, mutant enzyme Y343F
8.59
-
25°C, pH 8.0, 20 mM buffer, mutant enzyme Y343F
8.72
-
25°C, pH 8.25, 100 mM buffer, mutant enzyme Y343F
9.08
-
25°C, pH 8.25, 20 mM buffer, mutant enzyme Y343F
9.2
-
25°C, pH 8.5, 100 mM buffer, mutant enzyme Y343F
9.24
-
25°C, pH 8.5, 20 mM buffer, mutant enzyme Y343F
9.3
-
mutant R472Q, 25°C, pH 8.0
9.4
-
with ferricanide, pH 6.0, 25°C, recombinant mutant C242S/C253S/C260S/C451S
9.47
-
25°C, pH 9.0, 20 mM buffer, mutant enzyme Y343F
9.63
-
25°C, pH 9.5, 20 mM buffer, mutant enzyme Y343F
9.92
-
25°C, pH 9.75, 20 mM buffer, mutant enzyme Y343F
9.99
-
25°C, pH 9.0, 100 mM buffer, mutant enzyme Y343F
10.5
-
25°C, pH 9.5, 100 mM buffer, mutant enzyme Y343F
11.4
-
mutant V474M, 25°C, pH 7.0
12.1
-
25°C, pH 7.0, 100 mM buffer, wild-type enzyme
12.4
-
mutant V474M, 25°C, pH 10.0
12.8
-
mutant Y343N, 25°C, pH 7.0
13
-
mutant F79A, pH 8.0, 25°C
13
-
wild-type, 25°C, pH 10.0
13.2
-
25°C, pH 6.0, 20 mM buffer, wild-type enzyme
13.2
-
wild type enzyme in 20 mM Tris at pH 6.0
13.2
-
wild type enzyme, pH 6.0
13.2
-
wild-type, 25°C, pH 6.0
13.75
-
mutant Y343N, 25°C, pH 8.0
13.9
-
with ferricanide, pH 6.0, 25°C, recombinant wild-type enzyme
14.1
-
mutant R472D, pH 7.6, 25°C
15.1
-
mutant enzyme G473A, pH 10.0
15.5
-
mutant Y343N, 25°C, pH 9.0
15.8
-
mutant V474M, 25°C, pH 8.5
15.9
-
mutant enzyme G473A, pH 7.0
16
-
mutant F57A, pH 8.0, 25°C
16.2
-
with ferricanide, pH 7.1, 25°C, recombinant wild-type enzyme
16.9
-
mutant Y343N, 25°C, pH 8.5
17.1
-
mutant V474M, 25°C, pH 9.5
17.2
-
25°C, pH 7.5, 100 mM buffer, wild-type enzyme
17.7
-
25°C, pH 6.5, 20 mM buffer, wild-type enzyme
17.7
-
wild type enzyme, pH 6.5
17.8
-
mutant V474M, 25°C, pH 8.0
18.5
-
mutant R472K, pH 7.6, 25°C
19
-
mutant F57Y, pH 8.0, 25°C
19
-
mutant H90F, pH 8.0, 25°C
19.6
-
mutant V474M, 25°C, pH 9.0
19.8
-
with ferricanide, pH 7.1, 25°C, recombinant mutant C242S/C253S/C260S/C451S
20.8
-
with ferricanide, pH 7.1, 25°C, recombinant wild-type enzyme
21.6
-
mutant enzyme G473A, pH 7.4
23
-
mutant D342K, pH 7.6, 25°C
23.6
-
25°C, pH 8.75, 20 mM buffer, wild-type enzyme
24.2
-
25°C, pH 7.0, 20 mM buffer, wild-type enzyme
24.2
-
wild type enzyme, pH 7.0
24.2
-
wild-type, 25°C, pH 7.0
24.6
-
25°C, pH 9.5, 100 mM buffer, wild-type enzyme
24.7
-
25°C, pH 7.5, 20 mM buffer, wild-type enzyme
24.7
-
wild type enzyme, pH 7.5
24.8
-
25°C, pH 8.25, 20 mM buffer, wild-type enzyme
25
-
25°C, pH 8.0, 100 mM buffer, wild-type enzyme
25.7
-
25°C, pH 9.0, 20 mM buffer, wild-type enzyme
25.7
-
wild type enzyme, pH 9.0
25.7
-
wild-type, 25°C, pH 9.0
25.8
-
with ferricanide, pH 8.4, 25°C, recombinant mutant C242S/C253S/C260S/C451S
25.9
-
25°C, pH 8.0, 20 mM buffer, wild-type enzyme
25.9
-
wild type enzyme, pH 8.0
25.9
-
wild-type, 25°C, pH 8.0
26.1
-
with ferricanide, pH 6.0, 25°C, recombinant selenomethionine-labeled mutant C242S/C253S/C260S/C451S
26.2
-
mutant enzyme G473A, pH 8.0
26.3
-
25°C, pH 9.5, 20 mM buffer, wild-type enzyme
26.3
-
wild type enzyme, pH 9.5
26.3
-
wild-type, 25°C, pH 9.5
26.6
-
mutant R472Q, pH 7.6, 25°C
26.9
-
25°C, pH 8.5, 100 mM buffer, wild-type enzyme
26.9
-
25°C, pH 8.5, 20 mM buffer, wild-type enzyme
26.9
-
wild type enzyme in 20 mM Tris at pH 8.5
26.9
-
wild type enzyme, pH 8.5
26.9
-
wild-type, 25°C, pH 8.5
26.9
-
wild-type, pH 8.0, 25°C
27
-
25°C, pH 8.25, 100 mM buffer, wild-type enzyme
27
-
mutant R472M, pH 7.6, 25°C
27
-
wild-type, pH 8.0, 25°C
27.3
-
with ferricanide, pH 8.9, 25°C, recombinant wild-type enzyme
28.1
-
25°C, pH 9.0, 100 mM buffer, wild-type enzyme
28.4
-
mutant enzyme G473A in 20 mM Tris at pH 8.5
28.4
-
mutant enzyme G473A, pH 8.5
31.8
-
with ferricanide, pH 8.9, 25°C, recombinant selenomethionine-labeled mutant C242S/C253S/C260S/C451S
31.9
-
mutant enzyme G473A, pH 9.1
32.4
-
with ferricanide, pH 8.4, 25°C, recombinant wild-type enzyme
36.1
-
wild-type, 25°C, pH 7.0
36.2
-
with ferricanide, pH 8.9, 25°C, recombinant mutant C242S/C253S/C260S/C451S
37.7
-
with ferricanide, pH 7.1, 25°C, recombinant selenomethionine-labeled mutant C242S/C253S/C260S/C451S
41.2
-
with ferricanide, pH 8.4, 25°C, recombinant selenomethionine-labeled mutant C242S/C253S/C260S/C451S
42
-
mutant H90Y, pH 8.0, 25°C
46.6
-
with ferricanide, pH 7.1, 25°C, recombinant selenomethionine-labeled mutant C242S/C253S/C260S/C451S
47
-
mutant R472D/D342K, pH 7.6, 25°C
73
-
wild-type, 25°C, pH 8.5
368
-
cosubstrates sulfide, ubiquinone-1, pH 7.5, 25°C
368
-
with coenzyme Q and sulfide as cosubstrates, at pH 8.0 and 25°C
52.9
-
pH 8.3, 55°C, with decyl-ubiquinone
567
-
pH 8.3, 55°C, with Nitro-blue tetrazolium
382
-
solubilized enzyme, at pH 6.8 and 25°C
650
-
enzyme in nanodiscs, at pH 6.8 and 25°C
0.078
-
truncated protein, residues 76-679, pH not specified in the publication, temperature not specified in the publication
0.133
-
mutant G212S/L213T/Y214L/S217C/C220I/S221N, pH not specified in the publication, temperature not specified in the publication
1.93
-
truncated protein, residues 19-731, pH not specified in the publication, temperature not specified in the publication
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
60
-
in 0.1 M K+-phosphate buffer, at pH 7.0 and 30°C
0.0122
-
within a sulfite concentration range of 0.0014-0.3 mM and with a fixed reduced F420 concentration of 0.04 mM
0.000004
-
25°C, 20 mM Tris acetate buffer, pH 6.0, mutant Y236F
0.000007
-
25°C, 20 mM Tris acetate buffer, pH 6.5, mutant Y236F
0.0006
-
25°C, 20 mM Tris acetate buffer, pH 6.0, wild-type enzyme
0.0011
-
25°C, 20 mM Tris acetate buffer, pH 6.5, wild-type enzyme
0.002
-
pH 6.5, reaction with cytochrome c
0.0026
-
tether deletion mutant enzyme DELTAKVATV, in 20 mM Tris acetate, pH 8.0, at 25°C
0.0037
-
25°C, 20 mM Tris acetate buffer, pH 7.0, wild-type enzyme
0.00456
-
wild type enzyme, pH 7.0, using ferricyanide as the electron acceptor
0.006
-
mutant enzyme P105A, in 20 mM Tris acetate, pH 8.0, at 25°C
0.00605
-
mutant enzyme G473A, pH 7.0, using ferricyanide as the electron acceptor
0.0071
-
25°C, 20 mM Tris acetate buffer, pH 7.5, wild-type enzyme
0.01
-
reaction with yeast ferricytochrome c
0.0111
-
wild type enzyme, in 20 mM Tris acetate, pH 8.0, at 25°C
0.012
-
at pH 7.7 and 294 mV
0.0125
-
at pH 7.7 and 360 mV
0.0149
-
wild type enzyme, pH 8.6, using ferricyanide as the electron acceptor
0.022
-
25°C, 20 mM Tris acetate buffer, pH 8.0, wild-type enzyme
0.026
-
25°C, 20 mM Tris acetate buffer, pH 7.0, mutant Y236F
0.026
-
tether deletion mutant enzyme DELTAKVAT, in 20 mM Tris acetate, pH 8.0, at 25°C
0.032
-
mutant enzyme P105A/P111A, in 20 mM Tris acetate, pH 8.0, at 25°C
0.032
-
recombinant enzyme
0.033
-
mutant enzyme P111A, in 20 mM Tris acetate, pH 8.0, at 25°C
0.04
-
pH 8.0, reaction with cytochrome c
0.042
-
25°C, 20 mM Tris acetate buffer, pH 7.5, mutant Y236F
0.042
-
tether deletion mutant enzyme DELTAKVA, in 20 mM Tris acetate, pH 8.0, at 25°C
0.054
-
at pH 8.0 and 345 mV
0.06
-
reaction with cytochrome c
0.063
-
at pH 8.0 and 264 mV
0.086
-
25°C, 20 mM Tris acetate buffer, pH 8.5, wild-type enzyme
0.114
-
25°C, 20 mM Tris acetate buffer, pH 8.0, mutant Y236F
0.189
-
25°C, 20 mM Tris acetate buffer, pH 7.0, mutant H57A
0.191
-
mutant enzyme G473A, pH 8.5, using ferricyanide as the electron acceptor
0.22
-
25°C, 20 mM Tris acetate buffer, pH 7.5, mutant H57A
0.269
-
at pH 8.5 and 345 mV
0.27
-
25°C, 20 mM Tris acetate buffer, pH 8.0, mutant H57A
0.283
-
at pH 8.5 and 283 mV
0.29
-
25°C, 20 mM Tris acetate buffer, pH 6.5, mutant H57A
0.324
-
25°C, 20 mM Tris acetate buffer, pH 9.0, wild-type enzyme
0.332
-
25°C, 20 mM Tris acetate buffer, pH 8.5, mutant Y236Fyme
0.452
-
25°C, 20 mM Tris acetate buffer, pH 8.5, mutant H57A
0.54
-
reaction with ferricyanide
0.58
-
reaction with cytochrome c
0.667
-
25°C, 20 mM Tris acetate buffer, pH 6.0, mutant H57A
0.812
-
25°C, 20 mM Tris acetate buffer, pH 5.8, mutant R55M
1
-
reaction with ferricyanide
1.087
-
25°C, 20 mM Tris acetate buffer, pH 6.2, mutant R55M
1.155
-
25°C, 20 mM Tris acetate buffer, pH 9.0, mutant Y236F
1.46
-
25°C, 20 mM Tris acetate buffer, pH 9.0, mutant H57A
1.63
-
25°C, 20 mM Tris acetate buffer, pH 6.6, mutant R55M
1.66
-
25°C, 20 mM Tris acetate buffer, pH 9.5, wild-type enzyme
1.95
-
25°C, 20 mM Tris acetate buffer, pH 7.0, mutant R55M
3.39
-
25°C, 20 mM Tris acetate buffer, pH 10.0, wild-type enzyme
3.62
-
25°C, 20 mM Tris acetate buffer, pH 7.5, mutant R55M
4.456
-
25°C, 20 mM Tris acetate buffer, pH 9.5, mutant Y236F
8.17
-
25°C, 20 mM Tris acetate buffer, pH 7.9, mutant R55M
12.2
-
25°C, 20 mM Tris acetate buffer, pH 9.5, mutant H57A
15.49
-
25°C, 20 mM Tris acetate buffer, pH 10.0, mutant Y236F
21.2
-
mutant enzyme G473W, pH 7.0, using ferricyanide as the electron acceptor
24
-
mutant enzyme G473W, pH 8.5, using ferricyanide as the electron acceptor
26.5
-
mutant enzyme G473D, pH 7.0, using ferricyanide as the electron acceptor
41.4
-
mutant enzyme G473D, pH 8.5, using ferricyanide as the electron acceptor
0.00046
-
mutant V474M, 25°C, pH 6.0
0.00129
-
25°C, pH 6.0, 20 mM buffer, wild-type enzyme
0.00129
-
wild type enzyme in 20 mM Tris at pH 6.0
0.00129
-
wild type enzyme, pH 6.0
0.0013
-
wild-type, 25°C, pH 6.0
0.00133
-
wild-type, 25°C, pH 7.0
0.00134
-
mutant V474M, 25°C, pH 7.0
0.00162
-
25°C, pH 6.5, 20 mM buffer, wild-type enzyme
0.00162
-
wild type enzyme, pH 6.5
0.0017
-
mutant Y83A, pH 8.0, 25°C
0.0019
-
mutant R472M, 25°C, pH 6.0
0.0023
-
mutant R472Q, 25°C, pH 6.0
0.0025
-
mutant R472M, 25°C, pH 7.0
0.0027
-
wild-type, 25°C, pH 7.0
0.00272
-
25°C, pH 7.0, 20 mM buffer, wild-type enzyme
0.00272
-
wild type enzyme, pH 7.0
0.0028
-
mutant H90F, pH 8.0, 25°C
0.00311
-
25°C, pH 6.0, 20 mM buffer, mutant enzyme Y343F
0.0032
-
mutant R472K, pH 7.6, 25°C
0.0032
-
mutant V474M, 25°C, pH 8.0
0.00339
-
25°C, pH 7.5, 20 mM buffer, wild-type enzyme
0.00339
-
wild type enzyme, pH 7.5
0.0035
-
mutant R472Q, 25°C, pH 7.0
0.00352
-
25°C, pH 7.0, 100 mM buffer, mutant enzyme Y343F
0.00362
-
25°C, pH 7.0, 100 mM buffer, wild-type enzyme
0.00367
-
25°C, pH 7.5, 100 mM buffer, wild-type enzyme
0.0038
-
mutant F79A, pH 8.0, 25°C
0.0039
-
mutant H90Y, pH 8.0, 25°C
0.004
-
with ferricanide, pH 7.1, 25°C, recombinant wild-type enzyme
0.00414
-
25°C, pH 6.5, 20 mM buffer, mutant enzyme Y343F
0.0042
-
with ferricanide, pH 7.1, 25°C, recombinant wild-type enzyme
0.00423
-
25°C, pH 7.5, 100 mM buffer, mutant enzyme Y343F
0.0043
-
wild-type, 25°C, pH 8.0
0.00435
-
25°C, pH 8.0, 20 mM buffer, wild-type enzyme
0.00435
-
wild type enzyme, pH 8.0
0.00453
-
mutant enzyme G473A in 20 mM Tris at pH 6.0
0.00453
-
mutant enzyme G473A, pH 6.0
0.00459
-
25°C, pH 7.0, 20 mM buffer, mutant enzyme Y343F
0.0047
-
mutant R472M, 25°C, pH 8.0
0.0048
-
with ferricanide, pH 7.1, 25°C, recombinant mutant C242S/C253S/C260S/C451S
0.00503
-
25°C, pH 8.25, 20 mM buffer, wild-type enzyme
0.00536
-
mutant enzyme G473A, pH 7.0
0.00612
-
25°C, pH 8.0, 100 mM buffer, wild-type enzyme
0.0062
-
with ferricanide, pH 7.1, 25°C, recombinant mutant C242S/C253S/C260S/C451S
0.00635
-
25°C, pH 7.5, 20 mM buffer, mutant enzyme Y343F
0.00728
-
25°C, pH 8.25, 100 mM buffer, wild-type enzyme
0.0079
-
with ferricanide, pH 7.1, 25°C, recombinant selenomethionine-labeled mutant C242S/C253S/C260S/C451S
0.008
-
mutant F57Y, pH 8.0, 25°C
0.008
-
wild-type, 25°C, pH 8.5
0.0082
-
with ferricanide, pH 7.1, 25°C, recombinant selenomethionine-labeled mutant C242S/C253S/C260S/C451S
0.00825
-
25°C, pH 8.5, 20 mM buffer, wild-type enzyme
0.00825
-
wild type enzyme in 20 mM Tris at pH 8.5
0.00825
-
wild type enzyme, pH 8.5
0.0083
-
wild-type, 25°C, pH 8.5
0.00851
-
25°C, pH 10.0, 20 mM buffer, mutant enzyme Y343F
0.00859
-
25°C, pH 8.0, 20 mM buffer, mutant enzyme Y343F
0.00908
-
25°C, pH 8.25, 20 mM buffer, mutant enzyme Y343F
0.00924
-
25°C, pH 8.5, 20 mM buffer, mutant enzyme Y343F
0.00947
-
25°C, pH 9.0, 20 mM buffer, mutant enzyme Y343F
0.00959
-
25°C, pH 8.75, 20 mM buffer, wild-type enzyme
0.0096
-
with ferricanide, pH 6.0, 25°C, recombinant mutant C242S/C253S/C260S/C451S
0.00963
-
25°C, pH 9.5, 20 mM buffer, mutant enzyme Y343F
0.00992
-
25°C, pH 9.75, 20 mM buffer, mutant enzyme Y343F
0.0107
-
with ferricanide, pH 8.4, 25°C, recombinant mutant C242S/C253S/C260S/C451S
0.011
-
25°C, pH 8.5, 100 mM buffer, wild-type enzyme
0.011
-
wild-type, pH 8.0, 25°C
0.012
-
mutant D342K, pH 7.6, 25°C
0.012
-
mutant Y83F, pH 8.0, 25°C
0.0121
-
with ferricanide, pH 6.0, 25°C, recombinant wild-type enzyme
0.013
-
mutant F57A, pH 8.0, 25°C
0.013
-
mutant R472Q, 25°C, pH 8.0
0.0144
-
mutant V474M, 25°C, pH 9.0
0.0146
-
with ferricanide, pH 8.4, 25°C, recombinant selenomethionine-labeled mutant C242S/C253S/C260S/C451S
0.0158
-
25°C, pH 8.0, 100 mM buffer, mutant enzyme Y343F
0.016
-
mutant R472Q, 25°C, pH 8.5
0.0166
-
with ferricanide, pH 8.4, 25°C, recombinant wild-type enzyme
0.0172
-
mutant enzyme G473A, pH 7.4
0.0174
-
with ferricanide, pH 8.9, 25°C, recombinant selenomethionine-labeled mutant C242S/C253S/C260S/C451S
0.019
-
with ferricanide, pH 6.0, 25°C, recombinant selenomethionine-labeled mutant C242S/C253S/C260S/C451S
0.0196
-
with ferricanide, pH 8.9, 25°C, recombinant wild-type enzyme
0.021
-
mutant R472M, 25°C, pH 9.0
0.0214
-
pH not specified in the publication, temperature not specified in the publication
0.022
-
mutant R472Q, pH 7.6, 25°C
0.022
-
wild-type, 25°C, pH 9.0
0.0221
-
25°C, pH 9.0, 20 mM buffer, wild-type enzyme
0.0221
-
wild type enzyme, pH 9.0
0.0226
-
using ferricyanide as electron acceptor
0.023
-
mutant R472D, pH 7.6, 25°C
0.023
-
mutant R472D/D342K, pH 7.6, 25°C
0.026
-
25°C, pH 9.0, 100 mM buffer, wild-type enzyme
0.0288
-
with ferricanide, pH 8.9, 25°C, recombinant mutant C242S/C253S/C260S/C451S
0.0319
-
25°C, pH 8.25, 100 mM buffer, mutant enzyme Y343F
0.0319
-
mutant V474M, 25°C, pH 9.5
0.0338
-
using ferricyanide as electron acceptor
0.0354
-
mutant V474M, 25°C, pH 8.5
0.04
-
mutant V474M, 25°C, pH 10.0
0.042
-
mutant R472M, pH 7.6, 25°C
0.045
-
mutant R472Q, 25°C, pH 9.0
0.0487
-
mutant enzyme G473A, pH 8.0
0.051
-
mutant R472D, pH 6.5, 25°C
0.0529
-
wild-type, 25°C, pH 10.0
0.0536
-
25°C, pH 9.5, 100 mM buffer, wild-type enzyme
0.0537
-
mutant R472M, 25°C, pH 9.5
0.0557
-
25°C, pH 8.5, 100 mM buffer, mutant enzyme Y343F
0.0614
-
mutant Y343F/R472Q, 25°C, pH 6.0
0.067
-
wild-type, 25°C, pH 9.5
0.0671
-
25°C, pH 9.5, 20 mM buffer, wild-type enzyme
0.0671
-
wild type enzyme, pH 9.5
0.0692
-
mutant enzyme A208D in 20 mM Tris at pH 6.0
0.0877
-
mutant Y343F/R472Q, 25°C, pH 7.0
0.094
-
mutant R472M, 25°C, pH 8.5
0.0947
-
mutant Y343N, 25°C, pH 7.0
0.0953
-
mutant Y343N, 25°C, pH 6.0
0.0967
-
mutant R472Q, 25°C, pH 9.5
0.0969
-
mutant R472M, 25°C, pH 10.0
0.107
-
mutant enzyme G473A in 20 mM Tris at pH 8.5
0.107
-
mutant enzyme G473A, pH 8.5
0.147
-
25°C, pH 9.0, 100 mM buffer, mutant enzyme Y343F
0.181
-
mutant R472Q, 25°C, pH 10.0
0.2827
-
mutant Y343F/R472Q, 25°C, pH 8.0
0.297
-
mutant Y343N, 25°C, pH 8.0
0.33
-
mutant enzyme G473W, pH 7.0
0.59
1
25°C, pH 9.5, 100 mM buffer, mutant enzyme Y343F
0.623
-
mutant enzyme G473D, pH 7.0
0.712
-
mutant Y343F/R472Q, 25°C, pH 8.5
0.774
-
mutant enzyme G473A, pH 9.1
0.85
-
mutant Y343N, 25°C, pH 8.5
0.99
-
mutant enzyme G473D, pH 6.5
1.063
-
mutant enzyme G473D, pH 7.5
1.1
-
mutant Y343N/R472M/V474M, 25°C, pH 6.0
1.223
-
mutant enzyme G473D, pH 8.0
1.39
-
mutant enzyme A208D in 20 mM Tris at pH 8.5
1.42
-
mutant Y343N/R472M/V474M, 25°C, pH 7.0
1.54
-
25°C, pH 10.0, 100 mM buffer, mutant enzyme Y343F
1.66
-
mutant enzyme G473D in 20 mM Tris at pH 6.0
1.66
-
mutant enzyme G473D, pH 6.0
1.91
-
mutant enzyme G473W in 20 mM Tris at pH 6.0
1.91
-
mutant enzyme G473W, pH 6.0
2.03
-
mutant enzyme G473W in 20 mM Tris at pH 8.5
2.034
-
mutant enzyme G473W, pH 8.5
2.04
-
mutant enzyme G473D in 20 mM Tris at pH 8.5
2.04
-
mutant enzyme G473D, pH 8.5
2.14
-
mutant Y343N/R472M/V474M, 25°C, pH 8.0
2.46
-
mutant Y343N, 25°C, pH 9.0
3.34
-
mutant Y343F/R472Q, 25°C, pH 9.0
3.684
-
mutant enzyme G473A, pH 10.0
4.64
-
mutant Y343N/R472M, 25°C, pH 7.0
8.6
-
mutant Y322N/R450M, 25°C, pH 7.0
9.37
-
mutant Y343N, 25°C, pH 9.5
10.41
-
mutant enzyme G473W, pH 9.0
11.73
-
mutant Y322N/R450M, 25°C, pH 8.5
12
-
mutant Y343N, 25°C, pH 10.0
14
-
mutant Y343N/R472M/V474M, 25°C, pH 8.5
16.8
-
mutant Y343N/R472M, 25°C, pH 6.0
19.28
-
mutant Y343N/R472M, 25°C, pH 8.0
25.88
-
mutant enzyme G473D, pH 9.1
39.9
-
mutant Y343F/R472Q, 25°C, pH 9.5
42.99
-
mutant Y343N/R472M, 25°C, pH 8.5
55.5
-
mutant Y343N/R472M/V474M, 25°C, pH 9.0
59.6
-
mutant Y343F/R472Q, 25°C, pH 10.0
85.64
-
mutant Y343N/R472M, 25°C, pH 9.0
111
-
mutant Y343N/R472M/V474M, 25°C, pH 9.5
208
-
mutant Y343N/R472m, 25°C, pH 9.5
418
-
mutant Y343N/R472M/V474M, 25°C, pH 10.0
0.174
-
cosubstrates sulfide, ubiquinone-1, pH 7.5, 25°C
0.174
-
with coenzyme Q and sulfide as cosubstrates, at pH 8.0 and 25°C
0.0026
-
pH 8.3, 55°C, with decyl-ubiquinone
0.034
-
pH 8.3, 55°C, with Nitro-blue tetrazolium
0.19
-
solubilized enzyme, at pH 6.8 and 25°C
0.22
-
at pH 7.4 and 37°C
0.26
-
enzyme in nanodiscs, at pH 6.8 and 25°C
0.0064
-
mutant G212S/L213T/Y214L/S217C/C220I/S221N, pH not specified in the publication, temperature not specified in the publication
0.009
-
truncated protein, residues 76-679, pH not specified in the publication, temperature not specified in the publication
0.011
-
63K enzyme, reduced methyl viologen as electron donor
0.012
-
69K enzyme, reduced methyl viologen as electron donor
0.0122
-
within a sulfite concentration range of 0.0014-0.3 mM and with a fixed reduced coenzyme F420 concentration of 0.04 mM
0.021
-
63K enzyme, ferredoxin als electeron donor
0.025
-
69K enzyme, ferredoxin as electron donor
0.028
-
truncated protein, residues 19-731, pH not specified in the publication, temperature not specified in the publication
0.012
-
pH 7.0, temperature not specified in the publication
0.017
-
reaction with cytochrome c
0.071
-
reaction with cytochrome c
0.073
-
reaction with ferricyanide
0.093
-
reaction with cytochrome c
1.3
-
reaction with ferricyanide
1.5
-
reaction with ferricyanide
2.5
-
reaction with ferricyanide
3.1
-
pH 6.0, temperature not specified in the publication
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