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• • Molecular Structure
  • Sum Formula
  • BRENDA Name
  • Synonyms
  • InChIKey
  • Pathways
• Roles as Enzyme Ligand
• • In Vivo Substrate
  • In Vivo Product
  • Substrate
  • Product
  • Cofactor
  • Activator
  • Inhibitor
  • Metals and Ions
  • 3D Structure (PDB)
• Enzyme Kinetic Parameters
• • k_cat Value (Turnover Number)
  • K_M Value
  • K_i Value
  • IC₅₀ Value
• References & Links
• • References
  • Links

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Ligand vinorine

Basic Ligand Information

Molecular Structure

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Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (2 results)

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Show Natural Substrate (2 entries)

EC NUMBER

PROVEN IN VIVO REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

1.14.13.75

vinorine + NADPH + H+ + O2 = vomilenine + NADP+ + H2O

744036

-

1.14.14.104

vinorine + [reduced NADPH-hemoprotein reductase] + O2 = vomilenine + [oxidized NADPH-hemoprotein reductase] + H2O

744036, 440250

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In Vivo Product in Enzyme-catalyzed Reactions (1 result)

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Show Natural Product (1 entry)

EC NUMBER

PROVEN IN VIVO REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

2.3.1.160

acetyl-CoA + 16-epivellosimine = CoA + vinorine

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Substrate in Enzyme-catalyzed Reactions (2 results)

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Show Substrate (2 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

1.14.13.75

vinorine + NADPH + H+ + O2 = vomilenine + NADP+ + H2O

744036

-

1.14.14.104

vinorine + [reduced NADPH-hemoprotein reductase] + O2 = vomilenine + [oxidized NADPH-hemoprotein reductase] + H2O

440250, 744036

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Product in Enzyme-catalyzed Reactions (2 results)

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Show Product (2 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

2.3.1.160

acetyl-CoA + 16-epivellosimine = CoA + vinorine

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-

2.3.1.160

16-epi-vellosimine + acetyl-CoA = vinorine + CoA

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-

2.3.1.160

2 entries

Enzyme Kinetic Parameters

k_cat Value (Turnover Number) (1 result)

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Show Turnover Number (1 entry)

EC NUMBER

TURNOVER NUMBER [1/S]

TURNOVER NUMBER MAXIMUM [1/S]

COMMENTARY

LITERATURE

2.3.1.160

0.735

-

pH 7.0, 30°C, recombinant wild-type enzyme

661321

K_M Value (4 results)

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Show KM Value (4 entries)

EC NUMBER

KM VALUE [MM]

KM VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

1.14.13.75

0.0068

-

pH 6.5, 30°C, recombinant enzyme

744036

1.14.14.104

0.0068

-

pH 6.5, 30°C, recombinant enzyme

744036

1.14.14.104

26

-

-

440250

1.14.14.104

2 entries

2.3.1.160

0.01

-

pH 7.0, 30°C, recombinant wild-type enzyme

661321

References & Links

Literature References (3)

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Show Reference (3 entries)

BRENDA REFERENCE ID

PUBMED ID

TITLE

YEAR

AUTHORS

JOURNAL

VOLUME

PAGES

440250

-

Enzymic biosynthesis of vomilenine, a key intermediate of the ajmaline pathway, catalyzed by a novel cytochrome P450-dependent enzyme from plant cell cultures of Rauwolfia serpentina

1995

Falkenhagen, H.; Stoeckigt, J.

Z. Naturforsch. C

50

45-53

661321

15110860

Acetyltransfer in natural product biosynthesis - functional cloning and molecular analysis of vinorine synthase

2004

Bayer, A.; Ma, X.; Stockigt, J.

Bioorg. Med. Chem.

12

2787-2795

744036

28654178

Dual catalytic activity of a cytochrome P450 controls bifurcation at a metabolic branch point of alkaloid biosynthesis in Rauwolfia serpentina

2017

Dang, T.T.; Franke, J.; Tatsis, E.; O'Connor, S.E.

Angew. Chem. Int. Ed. Engl.

56

9440-9444

Links to other databases for vinorine

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Show Links (1 entry)

ChEBI

PubChem

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