General Stability | Organism |
---|---|
dialysis against 50 mM Tris-HCl buffer, stable after 5 h, 3% loss of activity after 1 day, 82% loss of activity after 6 days | Oryctolagus cuniculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Oryctolagus cuniculus | |
2,2'-dipyridyl | - |
Oryctolagus cuniculus | |
4-methoxypyrazole | - |
Oryctolagus cuniculus | |
Ag+ | - |
Oryctolagus cuniculus | |
Cu2+ | - |
Oryctolagus cuniculus | |
glutathione | - |
Oryctolagus cuniculus | |
Hg2+ | - |
Oryctolagus cuniculus | |
iodoacetic acid | - |
Oryctolagus cuniculus | |
NEM | - |
Oryctolagus cuniculus | |
PCMB | - |
Oryctolagus cuniculus | |
pyrazole | - |
Oryctolagus cuniculus | |
Zn2+ | - |
Oryctolagus cuniculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00694 | - |
NAD+ | pH 7.5 | Oryctolagus cuniculus | |
0.0531 | - |
NAD+ | pH 10.8 | Oryctolagus cuniculus | |
0.45 | - |
ethanol | pH 10.8 | Oryctolagus cuniculus | |
3.33 | - |
ethanol | pH 7.5 | Oryctolagus cuniculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zinc | contains 3.8 mol of Zn per mol of protein | Oryctolagus cuniculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
2 * 36000, SDS-PAGE | Oryctolagus cuniculus |
72000 | - |
gel filtration | Oryctolagus cuniculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Oryctolagus cuniculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Oryctolagus cuniculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.69 | - |
- |
Oryctolagus cuniculus |
Storage Stability | Organism |
---|---|
4°C, in presence of 1.0 mM dithiothreitol, stable for 10 days | Oryctolagus cuniculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
butanol + NAD+ | - |
Oryctolagus cuniculus | butyraldehyde + NADH | - |
? | |
ethanol + NAD+ | - |
Oryctolagus cuniculus | acetaldehyde + NADH | - |
? | |
methanol + NAD+ | no activity at pH 7.5, slight activity at pH 10.8 | Oryctolagus cuniculus | formaldehyde + NADH + H+ | - |
? | |
pentanol + NAD+ | - |
Oryctolagus cuniculus | n-pentanal + NADH | - |
? | |
propan-2-ol + NAD+ | - |
Oryctolagus cuniculus | acetone + NADH | - |
? | |
propanol + NAD+ | - |
Oryctolagus cuniculus | propionaldehyde + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 36000, SDS-PAGE | Oryctolagus cuniculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10.8 | - |
and a second lower maximum at pH 7.5 | Oryctolagus cuniculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Oryctolagus cuniculus | |
NADH | - |
Oryctolagus cuniculus | |
NADP+ | no activity as coenzyme | Oryctolagus cuniculus |