Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 15 mM, 85% inhibition | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0025 | - |
Anisaldehyde | - |
Saccharolobus solfataricus | |
0.013 | - |
benzyl alcohol | - |
Saccharolobus solfataricus | |
0.019 | - |
propan-1-ol | - |
Saccharolobus solfataricus | |
0.021 | - |
2-Methylcyclohexanol | - |
Saccharolobus solfataricus | |
0.05 | - |
pentan-2-ol | - |
Saccharolobus solfataricus | |
0.055 | - |
butan-1-ol | - |
Saccharolobus solfataricus | |
0.225 | - |
pentan-3-ol | - |
Saccharolobus solfataricus | |
0.25 | - |
(+/-)-3-methylcyclohexanone | - |
Saccharolobus solfataricus | |
0.26 | - |
ethanol | - |
Saccharolobus solfataricus | |
0.35 | - |
butan-2-ol | - |
Saccharolobus solfataricus | |
0.5 | - |
propan-2-ol | - |
Saccharolobus solfataricus | |
1.3 | - |
Cyclopentanone | - |
Saccharolobus solfataricus | |
6.6 | - |
acetone | - |
Saccharolobus solfataricus | |
7.5 | - |
3-methylbutan-2-one | - |
Saccharolobus solfataricus | |
14 | - |
butan-2-one | - |
Saccharolobus solfataricus | |
27 | - |
methanol | - |
Saccharolobus solfataricus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zinc | 2 mM required for optimal activity | Saccharolobus solfataricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
2 * 37000, SDS-PAGE | Saccharolobus solfataricus |
70000 | - |
gel filtration, sucrose density gradient centrifugation | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.92 | - |
- |
Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-methylcyclohexanol + NAD+ | - |
Saccharolobus solfataricus | (+)-2-methylcyclohexanone + NADH + H+ | - |
r | |
3-methylbutan-2-one + NADH | - |
Saccharolobus solfataricus | 3-methylbutan-2-ol + NAD+ | - |
? | |
3-methylcyclohexanol + NAD+ | - |
Saccharolobus solfataricus | 3-methylcyclohexanone + NADH | - |
r | |
3-methylcyclohexanone + NADH | - |
Saccharolobus solfataricus | 3-methylcyclohexanol + NAD+ | - |
r | |
acetone + NADH | - |
Saccharolobus solfataricus | isopropanol + NAD+ | - |
r | |
anisaldehyde + NADH + H+ | - |
Saccharolobus solfataricus | anisic alcohol + NAD+ | - |
? | |
benzyl alcohol + NAD+ | - |
Saccharolobus solfataricus | benzaldehyde + NADH | - |
? | |
butan-2-ol + NAD+ | - |
Saccharolobus solfataricus | butan-2-one + NADH | - |
? | |
butan-2-one + NADH | - |
Saccharolobus solfataricus | butan-2-ol + NAD+ | - |
r | |
butanol + NAD+ | - |
Saccharolobus solfataricus | butyraldehyde + NADH | - |
? | |
cyclopetanone + NADH + H+ | - |
Saccharolobus solfataricus | cyclopentanol + NAD+ | - |
? | |
ethanol + NAD+ | - |
Saccharolobus solfataricus | acetaldehyde + NADH + H+ | - |
? | |
methanol + NAD+ | - |
Saccharolobus solfataricus | formaldehyde + NADH + H+ | - |
r | |
pentan-2-ol + NAD+ | - |
Saccharolobus solfataricus | 2-pentanone + NADH | - |
? | |
pentan-3-ol + NAD+ | - |
Saccharolobus solfataricus | 3-pentanone + NADH | - |
? | |
propan-2-ol + NAD+ | - |
Saccharolobus solfataricus | acetone + NADH | - |
? | |
propanol + NAD+ | - |
Saccharolobus solfataricus | propionaldehyde + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 37000, SDS-PAGE | Saccharolobus solfataricus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 95 | continous increase in activity from 40°C to 95°C | Saccharolobus solfataricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
24 h, 30% loss of activity | Saccharolobus solfataricus |
60 | - |
half-life: 20 h | Saccharolobus solfataricus |
70 | - |
half-life: 5 h | Saccharolobus solfataricus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.317 | - |
acetone | - |
Saccharolobus solfataricus | |
0.983 | - |
Anisaldehyde | - |
Saccharolobus solfataricus | |
1.02 | - |
Cyclopentanone | - |
Saccharolobus solfataricus | |
1.22 | - |
3-methylbutan-2-one | - |
Saccharolobus solfataricus | |
1.68 | - |
benzyl alcohol | - |
Saccharolobus solfataricus | |
1.88 | - |
ethanol | - |
Saccharolobus solfataricus | |
1.93 | - |
butan-2-one | - |
Saccharolobus solfataricus | |
1.97 | - |
butan-1-ol | - |
Saccharolobus solfataricus | |
2.17 | - |
3-methylcyclohexanone | - |
Saccharolobus solfataricus | |
2.92 | - |
pentan-3-ol | - |
Saccharolobus solfataricus | |
3.1 | - |
methanol | - |
Saccharolobus solfataricus | |
3.55 | - |
butan-2-ol | - |
Saccharolobus solfataricus | |
3.82 | - |
pentan2-ol | - |
Saccharolobus solfataricus | |
4.03 | - |
propan-1-ol | - |
Saccharolobus solfataricus | |
6.92 | - |
propan-2-ol | - |
Saccharolobus solfataricus | |
7.72 | - |
3-methyl-cyclohexanol | - |
Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
reduction of anisaldehyde | Saccharolobus solfataricus |
8.5 | - |
oxidation of benzyl alcohol | Saccharolobus solfataricus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Saccharolobus solfataricus | |
NADH | - |
Saccharolobus solfataricus |