Literature summary for 1.1.1.1 extracted from

Sica, F.; Demasi, D.; Mazzarella, D.L.; Zagari, A.; Capasso, S.; Pearl, L.H.; D'Auria, S.; Raia, C.A.; Rossi, M.
Elimination of twinning in crystals of Sulfolobus sofataricus alcohol dehydrogenase holo-enzyme by growth in agarose gels (1994), Acta Crystallogr. Sect. D, 50, 508-511.

Crystallization (Commentary)

Crystallization (Comment)	Organism
twinned crystals are grown with the sitting drop vapour diffusion method using 2-methyl-2,4-pentanediol (50% v/v), Tris/HCl buffer (150 mM, pH 8.4), and NADH (1 mM), prismatic crystals are grown at 4°C and 20°C by microbatch and free interface diffusion methods with Tris/HCl buffer (130 mM, pH 8.0), NADH (2 mM), polyethyleneglycol 4000 (16% w/v), propan-2-ol or propan-1-ol (16% v/v) in trisodium citrate (100 mM, pH 4.8-5.6)	Saccharolobus solfataricus

Crystallization (Comment)

Organism

twinned crystals are grown with the sitting drop vapour diffusion method using 2-methyl-2,4-pentanediol (50% v/v), Tris/HCl buffer (150 mM, pH 8.4), and NADH (1 mM), prismatic crystals are grown at 4°C and 20°C by microbatch and free interface diffusion methods with Tris/HCl buffer (130 mM, pH 8.0), NADH (2 mM), polyethyleneglycol 4000 (16% w/v), propan-2-ol or propan-1-ol (16% v/v) in trisodium citrate (100 mM, pH 4.8-5.6)

Saccharolobus solfataricus

Organism

Organism	UniProt	Comment	Textmining
Saccharolobus solfataricus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Saccharolobus solfataricus

Synonyms

Synonyms	Comment	Organism
ADH	-	Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
95	-	stable and active up to 95°C	Saccharolobus solfataricus

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Saccharolobus solfataricus

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Release 2023.1 (January 2023)