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Literature summary for 1.1.1.1 extracted from

  • Raia, C.A.; Caruso, C.; Marino, M.; Vespa, N.; Rossi, M.
    Activation of Sulfolobus solfataricus alcohol dehydrogenase by modification of cysteine residue 38 with iodoacetic acid (1996), Biochemistry, 35, 638-647.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
sodium iodoacetate increasing concentrations od sodium iodoacetate produce a slight decrease in activity Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
NADH native enzyme, at 55°C Saccharolobus solfataricus
0.04
-
NADH carboxymethylated enzyme, at 55°C Saccharolobus solfataricus
0.045
-
Cyclohexanol native enzyme, at 55°C Saccharolobus solfataricus
0.12
-
4-bromobenzyl alcohol native enzyme, at 55°C Saccharolobus solfataricus
0.14
-
4-methoxybenzaldehyde native enzyme, at 55°C Saccharolobus solfataricus
0.14
-
3-bromobenzyl alcohol native enzyme, at 55°C Saccharolobus solfataricus
0.2
-
benzaldehyde carboxymethylated enzyme, at 55°C Saccharolobus solfataricus
0.2
-
NAD+ native enzyme, at 55°C Saccharolobus solfataricus
0.23
-
Cyclohexanol carboxymethylated enzyme, at 55°C Saccharolobus solfataricus
0.28
-
4-carboxybenzaldehyde native enzyme, at 55°C Saccharolobus solfataricus
0.3
-
benzaldehyde native enzyme, at 55°C Saccharolobus solfataricus
0.33
-
1-propanol native enzyme, at 55°C Saccharolobus solfataricus
0.4
-
4-bromobenzyl alcohol carboxymethylated enzyme, at 55°C Saccharolobus solfataricus
0.5
-
NAD+ carboxymethylated enzyme, at 55°C Saccharolobus solfataricus
0.6
-
2-propanol native enzyme, at 55°C Saccharolobus solfataricus
0.7
-
ethanol native enzyme, at 55°C Saccharolobus solfataricus
0.8
-
benzyl alcohol native enzyme, at 55°C Saccharolobus solfataricus
0.9
-
3-bromobenzyl alcohol carboxymethylated enzyme, at 55°C Saccharolobus solfataricus
1.2
-
3-Methoxybenzyl alcohol native enzyme, at 55°C Saccharolobus solfataricus
1.3
-
4-methoxybenzyl alcohol native enzyme, at 55°C Saccharolobus solfataricus
1.4
-
4-methoxybenzyl alcohol carboxymethylated enzyme, at 55°C Saccharolobus solfataricus
2
-
benzyl alcohol carboxymethylated enzyme, at 55°C Saccharolobus solfataricus
2.4
-
1-propanol carboxymethylated enzyme, at 55°C Saccharolobus solfataricus
3.3
-
3-Methoxybenzyl alcohol carboxymethylated enzyme, at 55°C Saccharolobus solfataricus
53
-
2-propanol carboxymethylated enzyme, at 55°C Saccharolobus solfataricus
184
-
ethanol carboxymethylated enzyme, at 55°C Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
-
strain DSM1617
-
Saccharolobus solfataricus DSM 1617
-
strain DSM1617
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information carboxymethylation with sodium iodoacetate Saccharolobus solfataricus

Purification (Commentary)

Purification (Comment) Organism
Blue A column chromatography Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-propanol + NAD+
-
Saccharolobus solfataricus propanal + NADH + H+
-
?
1-propanol + NAD+
-
Saccharolobus solfataricus DSM 1617 propanal + NADH + H+
-
?
2-propanol + NAD+
-
Saccharolobus solfataricus 2-propanone + NADH + H+
-
?
2-propanol + NAD+
-
Saccharolobus solfataricus DSM 1617 2-propanone + NADH + H+
-
?
3-bromobenzyl alcohol + NAD+
-
Saccharolobus solfataricus 3-bromobenzaldehyde + NADH + H+
-
?
3-methoxybenzyl alcohol + NAD+
-
Saccharolobus solfataricus 3-methoxybenzaldehyde + NADH + H+
-
?
4-bromobenzyl alcohol + NAD+
-
Saccharolobus solfataricus 4-bromobenzaldehyde + NADH + H+
-
?
4-carboxybenzaldehyde + NADH + H+
-
Saccharolobus solfataricus 4-carboxybenzyl alcohol + NAD+
-
?
4-methoxybenzyl alcohol + NAD+
-
Saccharolobus solfataricus 4-methoxybenzaldehyde + NADH + H+
-
r
4-nitrobenzaldehyde + NADH + H+
-
Saccharolobus solfataricus 4-nitrobenzyl alcohol + NAD+
-
?
benzyl alcohol + NAD+
-
Saccharolobus solfataricus benzaldehyde + NADH + H+
-
r
benzyl alcohol + NAD+
-
Saccharolobus solfataricus DSM 1617 benzaldehyde + NADH + H+
-
r
cyclohexanol + NAD+
-
Saccharolobus solfataricus cyclohexanone + NADH + H+
-
?
cyclohexanol + NAD+
-
Saccharolobus solfataricus DSM 1617 cyclohexanone + NADH + H+
-
?
ethanol + NAD+
-
Saccharolobus solfataricus acetaldehyde + NADH + H+
-
?
ethanol + NAD+
-
Saccharolobus solfataricus DSM 1617 acetaldehyde + NADH + H+
-
?

Synonyms

Synonyms Comment Organism
ADH
-
Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
the native enzyme remains completely stable after heating for 3 h at 55°C while the carboxymethylated enzyme loses 10% of activity under the same conditions Saccharolobus solfataricus
65
-
at 65°C the half-lives of the native and carboxymethylated enzymes are 9 h and 4 h, respectively Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.25
-
2-propanol native enzyme, at 55°C Saccharolobus solfataricus
0.45
-
2-propanol carboxymethylated enzyme, at 55°C Saccharolobus solfataricus
0.5
-
ethanol native enzyme, at 55°C Saccharolobus solfataricus
0.6
-
4-methoxybenzaldehyde native enzyme, at 55°C Saccharolobus solfataricus
0.65
-
Cyclohexanol native enzyme, at 55°C Saccharolobus solfataricus
0.7
-
4-carboxybenzaldehyde native enzyme, at 55°C Saccharolobus solfataricus
0.85
-
ethanol carboxymethylated enzyme, at 55°C Saccharolobus solfataricus
1.1
-
4-bromobenzyl alcohol native enzyme, at 55°C Saccharolobus solfataricus
1.2
-
Cyclohexanol carboxymethylated enzyme, at 55°C Saccharolobus solfataricus
1.2
-
1-propanol native enzyme, at 55°C Saccharolobus solfataricus
1.2
-
3-bromobenzyl alcohol native enzyme, at 55°C Saccharolobus solfataricus
1.4
-
NAD+ native enzyme, at 55°C Saccharolobus solfataricus
1.4
-
benzyl alcohol native enzyme, at 55°C Saccharolobus solfataricus
1.4
-
3-Methoxybenzyl alcohol native enzyme, at 55°C Saccharolobus solfataricus
1.5
-
4-methoxybenzyl alcohol native enzyme, at 55°C Saccharolobus solfataricus
1.8
-
benzaldehyde carboxymethylated enzyme, at 55°C Saccharolobus solfataricus
2
-
NADH carboxymethylated enzyme, at 55°C Saccharolobus solfataricus
2
-
3-bromobenzyl alcohol carboxymethylated enzyme, at 55°C Saccharolobus solfataricus
2.2
-
1-propanol carboxymethylated enzyme, at 55°C Saccharolobus solfataricus
3.5
-
4-bromobenzyl alcohol carboxymethylated enzyme, at 55°C Saccharolobus solfataricus
3.5
-
benzaldehyde native enzyme, at 55°C Saccharolobus solfataricus
4.5
-
NADH native enzyme, at 55°C Saccharolobus solfataricus
4.6
-
4-nitrobenzaldehyde native enzyme, at 55°C Saccharolobus solfataricus
14.5
-
benzyl alcohol carboxymethylated enzyme, at 55°C Saccharolobus solfataricus
16
-
3-Methoxybenzyl alcohol carboxymethylated enzyme, at 55°C Saccharolobus solfataricus
18.5
-
NAD+ carboxymethylated enzyme, at 55°C Saccharolobus solfataricus
20.5
-
4-methoxybenzyl alcohol carboxymethylated enzyme, at 55°C Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5 9.1
-
Saccharolobus solfataricus

Cofactor

Cofactor Comment Organism Structure
NAD+ dependent Saccharolobus solfataricus