Inhibitors | Comment | Organism | Structure |
---|---|---|---|
sodium iodoacetate | increasing concentrations od sodium iodoacetate produce a slight decrease in activity | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
NADH | native enzyme, at 55°C | Saccharolobus solfataricus | |
0.04 | - |
NADH | carboxymethylated enzyme, at 55°C | Saccharolobus solfataricus | |
0.045 | - |
Cyclohexanol | native enzyme, at 55°C | Saccharolobus solfataricus | |
0.12 | - |
4-bromobenzyl alcohol | native enzyme, at 55°C | Saccharolobus solfataricus | |
0.14 | - |
4-methoxybenzaldehyde | native enzyme, at 55°C | Saccharolobus solfataricus | |
0.14 | - |
3-bromobenzyl alcohol | native enzyme, at 55°C | Saccharolobus solfataricus | |
0.2 | - |
benzaldehyde | carboxymethylated enzyme, at 55°C | Saccharolobus solfataricus | |
0.2 | - |
NAD+ | native enzyme, at 55°C | Saccharolobus solfataricus | |
0.23 | - |
Cyclohexanol | carboxymethylated enzyme, at 55°C | Saccharolobus solfataricus | |
0.28 | - |
4-carboxybenzaldehyde | native enzyme, at 55°C | Saccharolobus solfataricus | |
0.3 | - |
benzaldehyde | native enzyme, at 55°C | Saccharolobus solfataricus | |
0.33 | - |
1-propanol | native enzyme, at 55°C | Saccharolobus solfataricus | |
0.4 | - |
4-bromobenzyl alcohol | carboxymethylated enzyme, at 55°C | Saccharolobus solfataricus | |
0.5 | - |
NAD+ | carboxymethylated enzyme, at 55°C | Saccharolobus solfataricus | |
0.6 | - |
2-propanol | native enzyme, at 55°C | Saccharolobus solfataricus | |
0.7 | - |
ethanol | native enzyme, at 55°C | Saccharolobus solfataricus | |
0.8 | - |
benzyl alcohol | native enzyme, at 55°C | Saccharolobus solfataricus | |
0.9 | - |
3-bromobenzyl alcohol | carboxymethylated enzyme, at 55°C | Saccharolobus solfataricus | |
1.2 | - |
3-Methoxybenzyl alcohol | native enzyme, at 55°C | Saccharolobus solfataricus | |
1.3 | - |
4-methoxybenzyl alcohol | native enzyme, at 55°C | Saccharolobus solfataricus | |
1.4 | - |
4-methoxybenzyl alcohol | carboxymethylated enzyme, at 55°C | Saccharolobus solfataricus | |
2 | - |
benzyl alcohol | carboxymethylated enzyme, at 55°C | Saccharolobus solfataricus | |
2.4 | - |
1-propanol | carboxymethylated enzyme, at 55°C | Saccharolobus solfataricus | |
3.3 | - |
3-Methoxybenzyl alcohol | carboxymethylated enzyme, at 55°C | Saccharolobus solfataricus | |
53 | - |
2-propanol | carboxymethylated enzyme, at 55°C | Saccharolobus solfataricus | |
184 | - |
ethanol | carboxymethylated enzyme, at 55°C | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
strain DSM1617 | - |
Saccharolobus solfataricus DSM 1617 | - |
strain DSM1617 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | carboxymethylation with sodium iodoacetate | Saccharolobus solfataricus |
Purification (Comment) | Organism |
---|---|
Blue A column chromatography | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-propanol + NAD+ | - |
Saccharolobus solfataricus | propanal + NADH + H+ | - |
? | |
1-propanol + NAD+ | - |
Saccharolobus solfataricus DSM 1617 | propanal + NADH + H+ | - |
? | |
2-propanol + NAD+ | - |
Saccharolobus solfataricus | 2-propanone + NADH + H+ | - |
? | |
2-propanol + NAD+ | - |
Saccharolobus solfataricus DSM 1617 | 2-propanone + NADH + H+ | - |
? | |
3-bromobenzyl alcohol + NAD+ | - |
Saccharolobus solfataricus | 3-bromobenzaldehyde + NADH + H+ | - |
? | |
3-methoxybenzyl alcohol + NAD+ | - |
Saccharolobus solfataricus | 3-methoxybenzaldehyde + NADH + H+ | - |
? | |
4-bromobenzyl alcohol + NAD+ | - |
Saccharolobus solfataricus | 4-bromobenzaldehyde + NADH + H+ | - |
? | |
4-carboxybenzaldehyde + NADH + H+ | - |
Saccharolobus solfataricus | 4-carboxybenzyl alcohol + NAD+ | - |
? | |
4-methoxybenzyl alcohol + NAD+ | - |
Saccharolobus solfataricus | 4-methoxybenzaldehyde + NADH + H+ | - |
r | |
4-nitrobenzaldehyde + NADH + H+ | - |
Saccharolobus solfataricus | 4-nitrobenzyl alcohol + NAD+ | - |
? | |
benzyl alcohol + NAD+ | - |
Saccharolobus solfataricus | benzaldehyde + NADH + H+ | - |
r | |
benzyl alcohol + NAD+ | - |
Saccharolobus solfataricus DSM 1617 | benzaldehyde + NADH + H+ | - |
r | |
cyclohexanol + NAD+ | - |
Saccharolobus solfataricus | cyclohexanone + NADH + H+ | - |
? | |
cyclohexanol + NAD+ | - |
Saccharolobus solfataricus DSM 1617 | cyclohexanone + NADH + H+ | - |
? | |
ethanol + NAD+ | - |
Saccharolobus solfataricus | acetaldehyde + NADH + H+ | - |
? | |
ethanol + NAD+ | - |
Saccharolobus solfataricus DSM 1617 | acetaldehyde + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ADH | - |
Saccharolobus solfataricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
the native enzyme remains completely stable after heating for 3 h at 55°C while the carboxymethylated enzyme loses 10% of activity under the same conditions | Saccharolobus solfataricus |
65 | - |
at 65°C the half-lives of the native and carboxymethylated enzymes are 9 h and 4 h, respectively | Saccharolobus solfataricus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
2-propanol | native enzyme, at 55°C | Saccharolobus solfataricus | |
0.45 | - |
2-propanol | carboxymethylated enzyme, at 55°C | Saccharolobus solfataricus | |
0.5 | - |
ethanol | native enzyme, at 55°C | Saccharolobus solfataricus | |
0.6 | - |
4-methoxybenzaldehyde | native enzyme, at 55°C | Saccharolobus solfataricus | |
0.65 | - |
Cyclohexanol | native enzyme, at 55°C | Saccharolobus solfataricus | |
0.7 | - |
4-carboxybenzaldehyde | native enzyme, at 55°C | Saccharolobus solfataricus | |
0.85 | - |
ethanol | carboxymethylated enzyme, at 55°C | Saccharolobus solfataricus | |
1.1 | - |
4-bromobenzyl alcohol | native enzyme, at 55°C | Saccharolobus solfataricus | |
1.2 | - |
Cyclohexanol | carboxymethylated enzyme, at 55°C | Saccharolobus solfataricus | |
1.2 | - |
1-propanol | native enzyme, at 55°C | Saccharolobus solfataricus | |
1.2 | - |
3-bromobenzyl alcohol | native enzyme, at 55°C | Saccharolobus solfataricus | |
1.4 | - |
NAD+ | native enzyme, at 55°C | Saccharolobus solfataricus | |
1.4 | - |
benzyl alcohol | native enzyme, at 55°C | Saccharolobus solfataricus | |
1.4 | - |
3-Methoxybenzyl alcohol | native enzyme, at 55°C | Saccharolobus solfataricus | |
1.5 | - |
4-methoxybenzyl alcohol | native enzyme, at 55°C | Saccharolobus solfataricus | |
1.8 | - |
benzaldehyde | carboxymethylated enzyme, at 55°C | Saccharolobus solfataricus | |
2 | - |
NADH | carboxymethylated enzyme, at 55°C | Saccharolobus solfataricus | |
2 | - |
3-bromobenzyl alcohol | carboxymethylated enzyme, at 55°C | Saccharolobus solfataricus | |
2.2 | - |
1-propanol | carboxymethylated enzyme, at 55°C | Saccharolobus solfataricus | |
3.5 | - |
4-bromobenzyl alcohol | carboxymethylated enzyme, at 55°C | Saccharolobus solfataricus | |
3.5 | - |
benzaldehyde | native enzyme, at 55°C | Saccharolobus solfataricus | |
4.5 | - |
NADH | native enzyme, at 55°C | Saccharolobus solfataricus | |
4.6 | - |
4-nitrobenzaldehyde | native enzyme, at 55°C | Saccharolobus solfataricus | |
14.5 | - |
benzyl alcohol | carboxymethylated enzyme, at 55°C | Saccharolobus solfataricus | |
16 | - |
3-Methoxybenzyl alcohol | carboxymethylated enzyme, at 55°C | Saccharolobus solfataricus | |
18.5 | - |
NAD+ | carboxymethylated enzyme, at 55°C | Saccharolobus solfataricus | |
20.5 | - |
4-methoxybenzyl alcohol | carboxymethylated enzyme, at 55°C | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | 9.1 | - |
Saccharolobus solfataricus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | dependent | Saccharolobus solfataricus |