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Literature summary for 1.1.1.1 extracted from

  • Cannio, R.; Fiorentino, G.; Carpinelli, P.; Rossi, M.; Bartolucci, S.
    Cloning and overexpression in Escherichia coli of the genes encoding NAD-dependent alcohol dehydrogenase from two Sulfolobus species (1996), J. Bacteriol., 178, 301-305.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Saccharolobus solfataricus
expressed in Escherichia coli Sulfolobus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0145
-
benzylalcohol in 50 mM glycine-NaOH at pH 10.0 Sulfolobus sp.
0.0172
-
ethanol in 50 mM glycine-NaOH at pH 10.0 Sulfolobus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
4 * 35000, gel filtration Saccharolobus solfataricus
35000
-
4 * 35000, gel filtration Sulfolobus sp.
140000
-
gel filtration Saccharolobus solfataricus
140000
-
gel filtration Sulfolobus sp.

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
-
strain DSM 1617
-
Sulfolobus sp. P50381
-
-
Sulfolobus sp. RC3 P50381
-
-

Purification (Commentary)

Purification (Comment) Organism
HiLoad Superdex 200 gel filtration Saccharolobus solfataricus
HiLoad Superdex 200 gel filtration Sulfolobus sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.22
-
crude extract, at pH 10.0 Sulfolobus sp.
0.78
-
crude extract, at pH 10.0 Saccharolobus solfataricus
2.88
-
after 12.9fold purification, at pH 10.0 Sulfolobus sp.
10.1
-
after 12.9fold purification, at pH 10.0 Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyl alcohol + NAD+
-
Saccharolobus solfataricus benzaldehyde + NADH + H+
-
?
benzyl alcohol + NAD+
-
Sulfolobus sp. benzaldehyde + NADH + H+
-
?
benzyl alcohol + NAD+
-
Sulfolobus sp. RC3 benzaldehyde + NADH + H+
-
?
ethanol + NAD+
-
Saccharolobus solfataricus acetaldehyde + NADH + H+
-
?
ethanol + NAD+
-
Sulfolobus sp. acetaldehyde + NADH + H+
-
?
ethanol + NAD+
-
Sulfolobus sp. RC3 acetaldehyde + NADH + H+
-
?

Subunits

Subunits Comment Organism
homotetramer 4 * 35000, gel filtration Saccharolobus solfataricus
homotetramer 4 * 35000, gel filtration Sulfolobus sp.

Synonyms

Synonyms Comment Organism
ADH
-
Saccharolobus solfataricus
ADH
-
Sulfolobus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
85 90 half-life of 3 h at 85°C and 1 h at 90°C Sulfolobus sp.
85 90 half-life of 3 h at 85°C and 1 h at 90°C, the catalytic efficiency is considerably higher at temperatures below 90°C Saccharolobus solfataricus

Cofactor

Cofactor Comment Organism Structure
NAD+ dependent Saccharolobus solfataricus
NAD+ dependent Sulfolobus sp.