Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Saccharolobus solfataricus |
expressed in Escherichia coli | Sulfolobus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0145 | - |
benzylalcohol | in 50 mM glycine-NaOH at pH 10.0 | Sulfolobus sp. | |
0.0172 | - |
ethanol | in 50 mM glycine-NaOH at pH 10.0 | Sulfolobus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
4 * 35000, gel filtration | Saccharolobus solfataricus |
35000 | - |
4 * 35000, gel filtration | Sulfolobus sp. |
140000 | - |
gel filtration | Saccharolobus solfataricus |
140000 | - |
gel filtration | Sulfolobus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
strain DSM 1617 | - |
Sulfolobus sp. | P50381 | - |
- |
Sulfolobus sp. RC3 | P50381 | - |
- |
Purification (Comment) | Organism |
---|---|
HiLoad Superdex 200 gel filtration | Saccharolobus solfataricus |
HiLoad Superdex 200 gel filtration | Sulfolobus sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.22 | - |
crude extract, at pH 10.0 | Sulfolobus sp. |
0.78 | - |
crude extract, at pH 10.0 | Saccharolobus solfataricus |
2.88 | - |
after 12.9fold purification, at pH 10.0 | Sulfolobus sp. |
10.1 | - |
after 12.9fold purification, at pH 10.0 | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzyl alcohol + NAD+ | - |
Saccharolobus solfataricus | benzaldehyde + NADH + H+ | - |
? | |
benzyl alcohol + NAD+ | - |
Sulfolobus sp. | benzaldehyde + NADH + H+ | - |
? | |
benzyl alcohol + NAD+ | - |
Sulfolobus sp. RC3 | benzaldehyde + NADH + H+ | - |
? | |
ethanol + NAD+ | - |
Saccharolobus solfataricus | acetaldehyde + NADH + H+ | - |
? | |
ethanol + NAD+ | - |
Sulfolobus sp. | acetaldehyde + NADH + H+ | - |
? | |
ethanol + NAD+ | - |
Sulfolobus sp. RC3 | acetaldehyde + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 35000, gel filtration | Saccharolobus solfataricus |
homotetramer | 4 * 35000, gel filtration | Sulfolobus sp. |
Synonyms | Comment | Organism |
---|---|---|
ADH | - |
Saccharolobus solfataricus |
ADH | - |
Sulfolobus sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | 90 | half-life of 3 h at 85°C and 1 h at 90°C | Sulfolobus sp. |
85 | 90 | half-life of 3 h at 85°C and 1 h at 90°C, the catalytic efficiency is considerably higher at temperatures below 90°C | Saccharolobus solfataricus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | dependent | Saccharolobus solfataricus | |
NAD+ | dependent | Sulfolobus sp. |