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Literature summary for 1.1.1.1 extracted from

  • Nie, Y.; Xu, Y.; Mu, X.Q.; Wang, H.Y.; Yang, M.; Xiao, R.
    Purification, characterization, gene cloning, and expression of a novel alcohol dehydrogenase with anti-prelog stereospecificity from Candida parapsilosis (2007), Appl. Environ. Microbiol., 73, 3759-3764.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.1

Cloned(Commentary)

Cloned (Comment) Organism
-
 Candida parapsilosis

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 1 mM, 99% loss of activity Candida parapsilosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 gel filtration Candida parapsilosis
31000
-
 1 * 31000, SDS-PAGE Candida parapsilosis

Organism

Organism UniProt Comment Textmining
Candida parapsilosis B2KJ46 fragment; CCTCC M203011
-

Purification (Commentary)

Purification (Comment) Organism
-
 Candida parapsilosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-butanone + NADPH + H+ 12.1% of the activity with 2-hydroxyacetophenone and NADPH Candida parapsilosis butan-2-ol + NADP+
-
 ?
2-heptanone + NADPH + H+ 6.8% of the activity with 2-hydroxyacetophenone and NADPH Candida parapsilosis heptan-2-ol + NADP+
-
 ?
2-hexanone + NADPH + H+ 5.9% of the activity with 2-hydroxyacetophenone and NADPH Candida parapsilosis hexan-2-ol + NADP+
-
 ?
2-hydroxyacetophenone + NADH + H+ 2.4% of the activity with NADPH Candida parapsilosis (S)-1-phenyl-1,2-ethanediol + NAD+
-
 ?
2-hydroxyacetophenone + NADPH + H+
-
 Candida parapsilosis (S)-1-phenyl-1,2-ethanediol + NADP+
-
 ?
2-octanone + NADPH + H+ 11.9% of the activity with 2-hydroxyacetophenone and NADPH Candida parapsilosis octan-2-ol + NADP+
-
 ?
2-pentanone + NADPH + H+ 11.9% of the activity with 2-hydroxyacetophenone and NADPH Candida parapsilosis pentan-2-ol + NADP+
-
 ?
3-pentanone + NADPH + H+ 7.1% of the activity with 2-hydroxyacetophenone and NADPH Candida parapsilosis pentan-3-ol + NADP+
-
 ?
acetone + NADPH + H+ 14.7% of the activity with 2-hydroxyacetophenone and NADPH Candida parapsilosis propan-2-ol + NADP+
-
 ?
acetophenone + NADPH + H+ 5.1% of the activity with 2-hydroxyacetophenone and NADPH Candida parapsilosis 1-phenylethanol + NADP+
-
 ?
benzoylformic acid + NADPH + H+ 2.4% of the activity with 2-hydroxyacetophenone and NADPH Candida parapsilosis mandelate + NADP+
-
 ?
ethyl 4-chloroacetoacetate + NADPH activity is 1.48fold higher than with 2-hydroxyacetophenone and NADPH Candida parapsilosis ?
-
 ?
ethyl trifluoroacetoacetate + NADPH + H+ 4.3% of the activity with 2-hydroxyacetophenone and NADPH Candida parapsilosis 1-ethoxy-2,2,2-trifluoroethanol + NADP+
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 31000, SDS-PAGE Candida parapsilosis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Candida parapsilosis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 50 20°C: about 80% of maximal activity, 50°C: about 55% of maximal activity Candida parapsilosis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 60 min, stable up to Candida parapsilosis
50
-
 60 min, about 50% loss of activity Candida parapsilosis
60
-
 60 min, complete loss of activity Candida parapsilosis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
 Candida parapsilosis

pH Range

pH Minimum pH Maximum Comment Organism
4 7.5 pH 4.0: about 80% of maximal activity, pH 7.5: about 60% of maximal activity Candida parapsilosis

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 8 4°C, 48 h, stable Candida parapsilosis