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Literature summary for 1.1.1.1 extracted from

  • Yamada-Onodera, K.; Fukui, M.; Tani, Y.
    Purification and characterization of alcohol dehydrogenase reducing N-benzyl-3-pyrrolidinone from Geotrichum capitatum (2007), J. Biosci. Bioeng., 103, 174-178.
    View publication on PubMed
Search for more literature for 1.1.1.1

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 1 mM, complete inhibition Magnusiomyces capitatus
2,2'-bipyridyl 1 mM, 78% inhibition Magnusiomyces capitatus
CoCl2 1 mM, 37% inhibition Magnusiomyces capitatus
CuCl2 1 mM, complete inhibition Magnusiomyces capitatus
FeCl3 0.1 mM, 13% inhibition Magnusiomyces capitatus
HgCl2 1 mM, complete inhibition Magnusiomyces capitatus
iodoacetic acid 1 mM, 10% inhibition Magnusiomyces capitatus
MgCl2 1 mM, 10% inhibition Magnusiomyces capitatus
NiCl2 1 mM, complete inhibition Magnusiomyces capitatus
PCMB 0.1 mM, complete inhibition Magnusiomyces capitatus
ZnSO4 1 mM, complete inhibition Magnusiomyces capitatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.13
-
 N-benzyl-3-pyrrolidinone pH 6.0 Magnusiomyces capitatus
0.79
-
 NAD+ pH 8.0 Magnusiomyces capitatus
0.87
-
 NADH pH 6.0 Magnusiomyces capitatus
8.47
-
 (S)-N-benzyl-3-pyrrolidinol pH 8.0 Magnusiomyces capitatus

Organism

Organism UniProt Comment Textmining
Magnusiomyces capitatus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Magnusiomyces capitatus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
31
-
-
 Magnusiomyces capitatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-butanediol + NAD+ 35% of the activity with (S)-N-benzyl-3-pyrrolidinol Magnusiomyces capitatus ?
-
 ?
1,2-hexanediol + NAD+ 20% of the activity with (S)-N-benzyl-3-pyrrolidinol Magnusiomyces capitatus ?
-
 ?
1,2-pentanediol + NAD+ 12% of the activity with (S)-N-benzyl-3-pyrrolidinol Magnusiomyces capitatus ?
-
 ?
1,3-butanediol + NAD+ 39% of the activity with (S)-N-benzyl-3-pyrrolidinol Magnusiomyces capitatus ?
-
 ?
1-hexanol + NAD+ 15% of the activity with (S)-N-benzyl-3-pyrrolidinol Magnusiomyces capitatus ?
-
 ?
1-hydroxy-2-butanone + NADH + H+ 59% of the activity with N-benzyl-3-pyrrolidinone Magnusiomyces capitatus butane-1,2-diol + NAD+
-
 ?
1-pentanol + NAD+ 8% of the activity with (S)-N-benzyl-3-pyrrolidinol Magnusiomyces capitatus pentanal + NADH + H+
-
 ?
1-phenyl-1-propanol + NAD+ 31% of the activity with (S)-N-benzyl-3-pyrrolidinol Magnusiomyces capitatus ?
-
 ?
2,3-butanediol + NAD+ 83% of the activity with (S)-N-benzyl-3-pyrrolidinol Magnusiomyces capitatus ?
-
 ?
2,3-butanedione + NADH + H+ activity is 1.6fold higher than with N-benzyl-3-pyrrolidinone Magnusiomyces capitatus ?
-
 ?
2,4-pentanediol + NAD+ 21% of the activity with (S)-N-benzyl-3-pyrrolidinol Magnusiomyces capitatus ?
-
 ?
2-butanol + NAD+ activity is 3.7fold higher than with (S)-N-benzyl-3-pyrrolidinol Magnusiomyces capitatus 2-butanone + NADH + H+
-
 r
2-butanone + NADH + H+ 40% of the activity with N-benzyl-3-pyrrolidinone Magnusiomyces capitatus 2-butanol + NAD+
-
 r
2-hexanol + NAD+ activity is 11.2fold higher than with (S)-N-benzyl-3-pyrrolidinol Magnusiomyces capitatus 2-hexanone + NADH + H+
-
 ?
2-hexanone + NADH + H+ activity is 1.6fold higher than with N-benzyl-3-pyrrolidinone Magnusiomyces capitatus ?
-
 ?
2-pentanol + NAD+ activity is 9fold higher than with (S)-N-benzyl-3-pyrrolidinol Magnusiomyces capitatus 2-pentanone + NADH + H+
-
 ?
2-propanol + NAD+ activity is 3.1fold higher than with (S)-N-benzyl-3-pyrrolidinol Magnusiomyces capitatus 2-propanone + NADH + H+
-
 ?
3,4-hexanedione + NADH + H+ 77% of the activity with N-benzyl-3-pyrrolidinone Magnusiomyces capitatus ?
-
 ?
acetophenone + NADH + H+ 40% of the activity with N-benzyl-3-pyrrolidinone Magnusiomyces capitatus 1-phenylethanol + NAD+
-
 ?
acetylacetone + NADH + H+ 63% of the activity with N-benzyl-3-pyrrolidinone Magnusiomyces capitatus ?
-
 ?
benzaldehyde + NADH + H+ 74% of the activity with N-benzyl-3-pyrrolidinone Magnusiomyces capitatus benzyl alcohol + NAD+
-
 ?
benzylacetone + NADH + H+ activity is 1.3fold higher than with N-benzyl-3-pyrrolidinone Magnusiomyces capitatus 4-phenylbutan-2-ol + NAD+
-
 ?
cyclohexanone + NADH + H+ activity is 2.1fold higher than with N-benzyl-3-pyrrolidinone Magnusiomyces capitatus ?
-
 ?
hydroxyacetone + NADH + H+ 27% of the activity with N-benzyl-3-pyrrolidinone Magnusiomyces capitatus propane-1,2-diol + NAD+
-
 ?
additional information no activity detected with: N-benzyl-2-pyrrolidinone, 2-pyrrolidinone, 3-hexanone, 4-hydroxy-2-butanone, (R)-N-benzyl-3-pyrrolidinol, ethanol, 1,3-propanediol, 1-butanol, 1,4-butanediol, 1,2,3-butanetriol, 1,2,4-butanetriol, acetol, 2-phenyl-1-propanol, 3-phenyl-1-propanol, benzyl alcohol and glycerol. No activity with NADP+ or NADPH Magnusiomyces capitatus ?
-
 ?
N-benzyl-3-pyrrolidinol + NAD+ 33% of the activity with (S)-N-benzyl-3-pyrrolidinol Magnusiomyces capitatus 1-benzylpyrrolidin-3-one + NADH + H+
-
 ?
N-benzyl-3-pyrrolidinone + NADH + H+ (S)-N-benzyl-3-pyrrolidinol is produced at an enantiomeric excess of more than 99.9% Magnusiomyces capitatus (S)-N-benzyl-3-pyrrolidinol + NAD+
-
 r
n-butylaldehyde + NADH + H+ activity is 1.8fold higher than with N-benzyl-3-pyrrolidinone Magnusiomyces capitatus butanal + NAD+
-
 ?
n-hexylaldehyde + NADH + H+ activity is 1.6fold higher than with N-benzyl-3-pyrrolidinone Magnusiomyces capitatus hexanal + NAD+
-
 ?
n-octylaldehyde + NADH + H+ activity is 1.7fold higher than with N-benzyl-3-pyrrolidinone Magnusiomyces capitatus n-octanol + NAD+
-
 ?
n-valeraldehyde + NADH + H+ activity is 2.8fold higher than with N-benzyl-3-pyrrolidinone Magnusiomyces capitatus n-pentanol + NAD+
-
 ?
propionaldehyde + NADH + H+ activity is 1.1fold higher than with N-benzyl-3-pyrrolidinone Magnusiomyces capitatus ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 reduction of N-benzyl-3-pyrrolidinone Magnusiomyces capitatus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 50 20°C: about 80% of maximal activity, 50°C: about 60% of maximal activity, reduction of N-benzyl-3-pyrrolidinone Magnusiomyces capitatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 7 reduction of N-benzyl-3-pyrrolidinone Magnusiomyces capitatus