Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 (DE3) pLysS cells | Thermoplasma acidophilum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.7 | - |
ethanol | in 100 mM Tris-HCl, at pH 5.0 and 75°C | Thermoplasma acidophilum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | Mg2+ does not have any effect on activity | Thermoplasma acidophilum | |
Zn2+ | maximum activity is reached at 0.5 mM Zn2+,Ta1316 ADH is able to tolerate high concentrations of Zn2+ | Thermoplasma acidophilum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
4 * 36000, Ta1316 ADH, SDS-PAGE | Thermoplasma acidophilum |
36010 | - |
4 * 36010, Ta1316 ADH, calculated from amino acid sequence | Thermoplasma acidophilum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoplasma acidophilum | Q9HIM3 | strain DSM 1728 | - |
Purification (Comment) | Organism |
---|---|
Sephacryl S-100 gel filtration | Thermoplasma acidophilum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
17.6 | - |
crude extract Ta1316 ADH with ethanol as a substrate, at pH 5.0 and 75°C | Thermoplasma acidophilum |
628.7 | - |
purified Ta1316 ADH with ethanol as a substrate, at pH 5.0 and 75°C | Thermoplasma acidophilum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-butanol + NAD+ | 67.7% activity compared to ethanol | Thermoplasma acidophilum | butanal + NADH + H+ | - |
? | |
1-hexanol + NAD+ | 15.3% activity compared to ethanol | Thermoplasma acidophilum | hexanal + NADH + H+ | - |
? | |
1-pentanol + NAD+ | 19.1% activity compared to ethanol | Thermoplasma acidophilum | pentanal + NADH + H+ | - |
? | |
2-pentanol + NAD+ | 5.7% activity compared to ethanol | Thermoplasma acidophilum | 2-pentanone + NADH + H+ | - |
? | |
2-propanol + NAD+ | 73.5% activity compared to ethanol | Thermoplasma acidophilum | 2-propanone + NADH + H+ | - |
? | |
ethanol + NAD+ | 100% activity | Thermoplasma acidophilum | acetaldehyde + NADH + H+ | - |
? | |
methanol + NAD+ | 49.9% activity compared to ethanol | Thermoplasma acidophilum | formaldehyde + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 36000, Ta1316 ADH, SDS-PAGE | Thermoplasma acidophilum |
homotetramer | 4 * 36010, Ta1316 ADH, calculated from amino acid sequence | Thermoplasma acidophilum |
Synonyms | Comment | Organism |
---|---|---|
ADH | - |
Thermoplasma acidophilum |
Ta1316 ADH | - |
Thermoplasma acidophilum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
- |
Thermoplasma acidophilum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 90 | - |
Thermoplasma acidophilum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Thermoplasma acidophilum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2 | 8 | - |
Thermoplasma acidophilum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | shows NAD+ as the preferred co-factor over NADP+ | Thermoplasma acidophilum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Thermoplasma acidophilum | Ta1316 ADH, calculated from amino acid sequence | - |
5.5 |