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Literature summary for 1.1.1.1 extracted from

  • Zhou, S.; Zhang, S.C.; Lai, D.Y.; Zhang, S.L.; Chen, Z.M.
    Biocatalytic characterization of a short-chain alcohol dehydrogenase with broad substrate specificity from thermophilic Carboxydothermus hydrogenoformans (2013), Biotechnol. Lett., 35, 359-365.
    View publication on PubMed
Search for more literature for 1.1.1.1

Cloned(Commentary)

Cloned (Comment) Organism
gene chy1186, overexpression in Escherichia coli strain BL21 (DE3), subcloning in Escherichia coli strain DH5alpha Carboxydothermus hydrogenoformans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten kinetics Carboxydothermus hydrogenoformans
5.3
-
 ethyl pyruvate pH 6.5, 50°C, recombinant enzyme Carboxydothermus hydrogenoformans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 x * 30000, SDS-PAGE Carboxydothermus hydrogenoformans

Organism

Organism UniProt Comment Textmining
Carboxydothermus hydrogenoformans
-
 gene chy1186
-
Carboxydothermus hydrogenoformans Z-2901 / DSM 6008
-
 gene chy1186
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme 1.5fold from Escherichia coli strain BL21 (DE3) Carboxydothermus hydrogenoformans

Source Tissue

Source Tissue Comment Organism Textmining
additional information optimal growth at 78°C Carboxydothermus hydrogenoformans
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.75
-
 purified recombinant enzyme, pH 6.5, 50°C Carboxydothermus hydrogenoformans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxo-3-methylpentane + NADH
-
 Carboxydothermus hydrogenoformans 3-methylpentan-2-ol + NAD+
-
 ?
2-oxo-3-methylpentane + NADH
-
 Carboxydothermus hydrogenoformans Z-2901 / DSM 6008 3-methylpentan-2-ol + NAD+
-
 ?
2-oxo-3-phenylpropane + NADH
-
 Carboxydothermus hydrogenoformans 2-hydroxy-3-phenylpropane + NAD+
-
 ?
2-oxo-3-phenylpropane + NADH
-
 Carboxydothermus hydrogenoformans Z-2901 / DSM 6008 2-hydroxy-3-phenylpropane + NAD+
-
 ?
2-oxo-4-methylpentane + NADH
-
 Carboxydothermus hydrogenoformans 4-methylpentan-2-ol + NAD+
-
 ?
2-oxohexane + NADH
-
 Carboxydothermus hydrogenoformans 2-hydroxyhexane + NAD+
-
 ?
2-oxopentane + NADH
-
 Carboxydothermus hydrogenoformans 2-pentanol + NAD+
-
 ?
3-methoxy-1-phenylpropan-1-one + NADH
-
 Carboxydothermus hydrogenoformans 3-methoxy-1-phenylpropan-1-ol + NAD+
-
 ?
3-oxoheptane + NADH
-
 Carboxydothermus hydrogenoformans 3-hydroxyheptane + NAD+
-
 ?
4-phenylbutan-2-one + NADH
-
 Carboxydothermus hydrogenoformans 4-phenylbutan-2-ol + NAD+
-
 ?
benzaldehyde + NADH
-
 Carboxydothermus hydrogenoformans benzyl alcohol + NAD+
-
 ?
benzaldehyde + NADH
-
 Carboxydothermus hydrogenoformans Z-2901 / DSM 6008 benzyl alcohol + NAD+
-
 ?
cycloheptanone + NADH
-
 Carboxydothermus hydrogenoformans cycloheptanol + NAD+
-
 ?
cycloheptanone + NADH
-
 Carboxydothermus hydrogenoformans Z-2901 / DSM 6008 cycloheptanol + NAD+
-
 ?
cyclooctanone + NADH
-
 Carboxydothermus hydrogenoformans cyclooctanol + NAD+
-
 ?
cyclopentanone + NADH
-
 Carboxydothermus hydrogenoformans cyclopentanol + NAD+
-
 ?
ethyl 3-oxobutanoate + NADH
-
 Carboxydothermus hydrogenoformans ethyl 3-hydroxybutanoate + NAD+
-
 ?
ethyl 3-oxohexanoate + NADH
-
 Carboxydothermus hydrogenoformans ethyl 3-hydroxyhexanoate + NAD+
-
 ?
ethyl 3-oxopentanoate + NADH
-
 Carboxydothermus hydrogenoformans ethyl 3-hydroxypentanoate + NAD+
-
 ?
ethyl 3-phenyl-3-oxopropanoate + NADH
-
 Carboxydothermus hydrogenoformans ethyl 3-phenyl-3-hydroxypropanoate + NAD+
-
 ?
ethyl benzoylformate + NADH enantiomeric excess of 99.9% Carboxydothermus hydrogenoformans ethyl (R)-mandelate + NAD+
-
 ?
ethyl pyruvate + NADH
-
 Carboxydothermus hydrogenoformans ethyl 2-hydroxypropanoate + NAD+
-
 ?
additional information the enzyme exhibits broad substrate specificity towards aliphatic ketones, cycloalkanones, aromatic ketones, and ketoesters Carboxydothermus hydrogenoformans ?
-
 ?
additional information the enzyme exhibits broad substrate specificity towards aliphatic ketones, cycloalkanones, aromatic ketones, and ketoesters Carboxydothermus hydrogenoformans Z-2901 / DSM 6008 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 30000, SDS-PAGE Carboxydothermus hydrogenoformans

Synonyms

Synonyms Comment Organism
CHY1186
-
 Carboxydothermus hydrogenoformans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
 Carboxydothermus hydrogenoformans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.97
-
 ethyl pyruvate pH 6.5, 50°C, recombinant enzyme Carboxydothermus hydrogenoformans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 reduction reaction Carboxydothermus hydrogenoformans

Cofactor

Cofactor Comment Organism Structure
additional information the enzyme prefers NAD(H) over NADP(H) as a cofactor Carboxydothermus hydrogenoformans
NAD+
-
 Carboxydothermus hydrogenoformans
NADH
-
 Carboxydothermus hydrogenoformans

General Information

General Information Comment Organism
evolution the enzyme belongs to the short-chain alcohol dehydrogenases Carboxydothermus hydrogenoformans