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Literature summary for 1.1.1.1 extracted from

  • Timpson, L.M.; Liliensiek, A.K.; Alsafadi, D.; Cassidy, J.; Sharkeym M.A.; Liddell, S.; Allers, T.; Paradisi, F.
    A comparison of two novel alcohol dehydrogenase enzymes (ADH1 and ADH2) from the extreme halophile Haloferax volcanii (2012), Appl. Microbiol. Biotechnol., 97, 195-203.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
homologously expressed Haloferax volcanii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.071
-
NADH pH 6.0, temperature not specified in the publication Haloferax volcanii
0.29
-
NAD+ pH 11.0, temperature not specified in the publication Haloferax volcanii
28
-
acetaldehyde pH 6.0, temperature not specified in the publication Haloferax volcanii
31
-
ethanol pH 11.0, temperature not specified in the publication Haloferax volcanii

Metals/Ions

Metals/Ions Comment Organism Structure
KCl optimally active with ethanol and 1-propanol at pH 11.0 with 3 M KCl and with 1-butanol at pH 10.0 with 4 M KCl. Catalyzes the reductive reaction optimally at pH 6.0 with 4 M KCl Haloferax volcanii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37600
-
4 * 37600, SDS-PAGE Haloferax volcanii
138000
-
gel filtration Haloferax volcanii

Organism

Organism UniProt Comment Textmining
Haloferax volcanii D4GSN2
-
-
Haloferax volcanii DSM 3757 D4GSN2
-
-

Purification (Commentary)

Purification (Comment) Organism
purified in one step by immobilized metal-affinity chromatography Haloferax volcanii

Storage Stability

Storage Stability Organism
-20°C, inactive after approximately 2 weeks, storage at -80°C or lyophilization does not improve storage time Haloferax volcanii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-propanol + NAD+ the enzyme shows a preference for short-chain alcohols ethanol and 1-propanol Haloferax volcanii propanal + NADH + H+
-
?
1-propanol + NAD+ the enzyme shows a preference for short-chain alcohols ethanol and 1-propanol Haloferax volcanii DSM 3757 propanal + NADH + H+
-
?
3-methylbutanal + NAD+ about 10% of the activity with ethanol or 1-propanol Haloferax volcanii ? + NADH + H+
-
?
3-methylbutanal + NAD+ about 10% of the activity with ethanol or 1-propanol Haloferax volcanii DSM 3757 ? + NADH + H+
-
?
butanol + NAD+ about 15% of the activity with ethanol or 1-propanol Haloferax volcanii 1-butanal + NADH + H+
-
?
ethanol + NAD+ the enzyme shows a preference for short-chain alcohols ethanol and 1-propanol Haloferax volcanii acetaldehyde + NADH + H+
-
?
ethanol + NAD+ the enzyme shows a preference for short-chain alcohols ethanol and 1-propanol Haloferax volcanii DSM 3757 acetaldehyde + NADH + H+
-
?
additional information the NAD+-dependent HvADH1 shows a preference for short-chain alcohols, no activity with methanol Haloferax volcanii ?
-
?
additional information the NAD+-dependent HvADH1 shows a preference for short-chain alcohols, no activity with methanol Haloferax volcanii DSM 3757 ?
-
?
pentanol + NAD+ about 10% of the activity with ethanol or 1-propanol Haloferax volcanii pentanal + NADH + H+
-
?
pentanol + NAD+ about 10% of the activity with ethanol or 1-propanol Haloferax volcanii DSM 3757 pentanal + NADH + H+
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 37600, SDS-PAGE Haloferax volcanii

Synonyms

Synonyms Comment Organism
HvADH1
-
Haloferax volcanii
HVO_2428 locus name Haloferax volcanii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
-
Haloferax volcanii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
60 90 60°C: 75% of maximal activity, 90°C: 75% of maximal activity Haloferax volcanii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
reduction of aldehyde Haloferax volcanii
10
-
optimally active with 1-butanol at pH 10.0 with 4 M KCl Haloferax volcanii
11
-
optimally active with ethanol and 1-propanol at pH 11.0 with 3 M KCl Haloferax volcanii

Cofactor

Cofactor Comment Organism Structure
NAD+ exclusively NAD+ dependent Haloferax volcanii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.048
-
acetaldehyde pH 6.0, temperature not specified in the publication Haloferax volcanii
0.072
-
ethanol pH 11.0, temperature not specified in the publication Haloferax volcanii
9.037
-
NAD+ pH 11.0, temperature not specified in the publication Haloferax volcanii
32.77
-
NADH pH 6.0, temperature not specified in the publication Haloferax volcanii