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Literature summary for 1.1.1.103 extracted from

  • Craig, P.A.; Dekker, E.E.
    Cd2+ activation of L-threonine dehydrogenase from Escherichia coli K-12 (1988), Biochim. Biophys. Acta, 957, 222-229.
    View publication on PubMed
Search for more literature for 1.1.1.103

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.11
-
 NAD+ metal-ion free enzyme Escherichia coli K-12
0.18
-
 NAD+ enzyme activated with 0.07 mM Cd2+ Escherichia coli K-12
0.6
-
 NAD+ enzyme activated with 0.25 mM Mn2+ Escherichia coli K-12
1.1
-
 L-threonine metal-ion free enzyme Escherichia coli K-12
8.2
-
 L-threonine enzyme activated with 0.07 mM Cd2+ Escherichia coli K-12
211
-
 L-threonine enzyme activated with 0.25 mM Mn2+ Escherichia coli K-12

Metals/Ions

Metals/Ions Comment Organism Structure
Cd2+ activity of metal-ion free enzyme increases 10fold Escherichia coli K-12

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-threonine + NAD+ Escherichia coli K-12
-
 (2S)-2-amino-3-oxobutanoate + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli K-12
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
28.3
-
 metal-ion free enzyme Escherichia coli K-12
243
-
 enzyme activated with 0.07 mM Cd2+ Escherichia coli K-12
685
-
 enzyme activated with 0.25 mM Mn2+ Escherichia coli K-12

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-threonine + NAD+
-
 Escherichia coli K-12 (2S)-2-amino-3-oxobutanoate + NADH + H+
-
 ?

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Escherichia coli K-12