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Literature summary for 1.1.1.103 extracted from

  • Zhang, L.; Cao, Y.; Tong, J.; Xu, Y.
    An alkylpyrazine synthesis mechanism involving L-threonine-3-dehydrogenase describes the production of 2,5-dimethylpyrazine and 2,3,5-trimethylpyrazine by Bacillus subtilis (2019), Appl. Environ. Microbiol., 85, e01807-19 .
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-threonine + NAD+ Bacillus subtilis
-
 L-2-amino-3-oxobutanoate + NADH + H+
-
 ?
L-threonine + NAD+ Bacillus subtilis 168
-
 L-2-amino-3-oxobutanoate + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis O31776
-
-
Bacillus subtilis 168 O31776
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-threonine + NAD+
-
 Bacillus subtilis L-2-amino-3-oxobutanoate + NADH + H+
-
 ?
L-threonine + NAD+ the product L-2-amino-3-oxobutanoate is unstable. It spontaneously converts to 2,5-dimethylpyrazine in a pH-dependent reaction, via 3,6-dihydro-2,5-dimethylpyrazine. The reaction from aminoacetone to 2,5-dimethylpyrazine is a pH-dependent nonenzymatic reaction. Inactivation of 2-amino-3-ketobutyrate coenzyme A ligase in Bacilus subtilis improves 2,5-dimethylpyrazine production Bacillus subtilis L-2-amino-3-oxobutanoate + NADH + H+
-
 ?
L-threonine + NAD+
-
 Bacillus subtilis 168 L-2-amino-3-oxobutanoate + NADH + H+
-
 ?
L-threonine + NAD+ the product L-2-amino-3-oxobutanoate is unstable. It spontaneously converts to 2,5-dimethylpyrazine in a pH-dependent reaction, via 3,6-dihydro-2,5-dimethylpyrazine. The reaction from aminoacetone to 2,5-dimethylpyrazine is a pH-dependent nonenzymatic reaction. Inactivation of 2-amino-3-ketobutyrate coenzyme A ligase in Bacilus subtilis improves 2,5-dimethylpyrazine production Bacillus subtilis 168 L-2-amino-3-oxobutanoate + NADH + H+
-
 ?

Synonyms

Synonyms Comment Organism
TDH
-
 Bacillus subtilis