KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.6 | - |
4-Oxoproline | - |
Oryctolagus cuniculus | |
0.84 | - |
NADH | - |
Oryctolagus cuniculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-oxo-proline + NADH | Oryctolagus cuniculus | - |
4-hydroxy-L-proline + NAD+ | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, negative alumina gel, positive alumina gel | Oryctolagus cuniculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Oryctolagus cuniculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5.21 | - |
- |
Oryctolagus cuniculus |
Storage Stability | Organism |
---|---|
-10°C, 160 mM phosphate buffer, pH 6.0, 3 months, no loss of activity | Oryctolagus cuniculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-oxo-proline + NADH | - |
Oryctolagus cuniculus | 4-hydroxy-L-proline + NAD+ | - |
ir | |
4-oxoproline + NADH | - |
Oryctolagus cuniculus | 4-hydroxy-L-proline + NAD+ | - |
ir |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 6.5 | decrease of activity below pH 6.0 | Oryctolagus cuniculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Oryctolagus cuniculus |