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Literature summary for 1.1.1.106 extracted from

  • Myhnen, T.; Mntsl, P.
    Evidence for the importance of cysteine and arginine residues in Pseudomonas fluorescens UK-1 pantoate dehydrogenase (1980), Biochim. Biophys. Acta, 614, 266-273.
    View publication on PubMed

General Stability

General Stability Organism
unstable without 2-mercaptoethanol, NAD+ stabilizes to a certain extent, the enzyme is unstable below pH 5.5 and above pH 10.0 even in the presence of NAD+ Pseudomonas fluorescens

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoic acid) 1 mM, 50% inhibition after 4.6 min, half-inactivation time increases to 7.0 min in the presence of 20 mM D-pantoate Pseudomonas fluorescens
iodacetic acid 1 mM, 50% inhibition after 14 min, half-inactivation time increases to 29.0 min in the presence of 20 mM D-pantoate, 70% inhibition within 50 min at pH 7.2, 20 mM pantoate decreases inactivation from 70 to 30% Pseudomonas fluorescens
p-chloromercuribenzoate 0.1 mM, 85% inhibition after 15 min, 200 mM 2-mercaptoethanol restores 70% of enzyme activity within 10 min, 20 mM D-pantoate and 1 mM NAD+ prevent inactivation when added simultaneously Pseudomonas fluorescens
Phenylglyoxal 5.3 mM, 70% inhibition in borate buffer, 92% inhibition in NaHCO3 buffer within 10 min, complete inactivation after prolonged incubation with phenylglyoxal, NAD+ and D-pantoate increase half-inactivation time from 6 min without substrates to 40 and 17 min respectively Pseudomonas fluorescens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.078
-
NAD+
-
Pseudomonas fluorescens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(R)-pantoate + NAD+ Pseudomonas fluorescens
-
(R)-4-dehydropantoate + NADH
-
?
(R)-pantoate + NAD+ Pseudomonas fluorescens UK1
-
(R)-4-dehydropantoate + NADH
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas fluorescens
-
UK1
-
Pseudomonas fluorescens UK1
-
UK1
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, 5'-AMP-Sepharose 4B Pseudomonas fluorescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-pantoate + NAD+
-
Pseudomonas fluorescens (R)-4-dehydropantoate + NADH
-
?
(R)-pantoate + NAD+
-
Pseudomonas fluorescens UK1 (R)-4-dehydropantoate + NADH
-
?

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Pseudomonas fluorescens