Cloned (Comment) | Organism |
---|---|
gene over-expressed in Saccharomyces cerevisiae S700 | Scheffersomyces stipitis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
NAD+ | - |
Scheffersomyces stipitis | |
20 | - |
D-arabinitol | - |
Scheffersomyces stipitis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no divalent cations are required for activity | Scheffersomyces stipitis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
4 * 30000, calculated from gene sequence and molecular weight of native enzyme | Scheffersomyces stipitis |
110000 | - |
native gradient PAGE | Scheffersomyces stipitis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-arabinitol + NAD+ | Scheffersomyces stipitis | recombinant protein from Saccharomyces cerevisiae | D-ribulose + NADH + H+ | - |
? | |
D-arabinitol + NAD+ | Scheffersomyces stipitis | D-arabitol i.e. D-arabinitol | D-xylulose + NADH + H+ | - |
? | |
additional information | Scheffersomyces stipitis | D-arabinitol dehydrogenases (ArDH) from fungi and yeast oxidize D-arabinitol to D-ribulose, whereas bacterial ArDH oxidizes D-arabinitol to D-xylulose | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Scheffersomyces stipitis | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.2 | - |
D-xylulose, of recombinant protein | Scheffersomyces stipitis |
0.78 | - |
- |
Scheffersomyces stipitis |
68.25 | - |
D-arabinitol, of recombinant protein | Scheffersomyces stipitis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-arabinitol + NAD+ | recombinant protein from Saccharomyces cerevisiae | Scheffersomyces stipitis | D-ribulose + NADH + H+ | - |
? | |
D-arabinitol + NAD+ | D-arabitol i.e. D-arabinitol | Scheffersomyces stipitis | D-xylulose + NADH + H+ | - |
? | |
D-glucitol + NAD+ | recombinant protein from Saccharomyces cerevisiae and enzyme from Pichia stipitis | Scheffersomyces stipitis | ? | - |
? | |
Galactitol + NAD+ | recombinant protein from Saccharomyces cerevisiae | Scheffersomyces stipitis | ? | - |
? | |
additional information | D-arabinitol dehydrogenases (ArDH) from fungi and yeast oxidize D-arabinitol to D-ribulose, whereas bacterial ArDH oxidizes D-arabinitol to D-xylulose | Scheffersomyces stipitis | ? | - |
? | |
Xylitol + NAD+ | recombinant protein from Saccharomyces cerevisiae and enzyme from Pichia stipitis | Scheffersomyces stipitis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 30000, calculated from gene sequence and molecular weight of native enzyme | Scheffersomyces stipitis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no activity with NADPH | Scheffersomyces stipitis |