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Literature summary for 1.1.1.114 extracted from
- D-Apiitol dehydrogenase from bacteria (1982), Methods Enzymol., 89, 228-232.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| NADH | 0.1 mM | Micrococcus sp. |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.015 | - |
NADH | - |
Micrococcus sp. | |
| 0.35 | - |
NAD+ | - |
Micrococcus sp. | |
| 11.6 | - |
D-apiitol | - |
Micrococcus sp. | |
| 71.4 | - |
D-apiose | - |
Micrococcus sp. | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 115000 | - |
sucrose density centrifugation, gel filtration | Micrococcus sp. |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-apiitol + NAD+ | Micrococcus sp. | - |
D-apiose + NADH | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Micrococcus sp. | - |
gram-negativ coccus isolated from the surface of germinating parsley seeds by enrichment culture | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| streptomycin sulfate, calcium phosphate gel, ethanol precipitation, DEAE-cellulose, Sephadex G-200 | Micrococcus sp. |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.102 | - |
- |
Micrococcus sp. |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, several weeks, no loss of activity | Micrococcus sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-apiitol + NAD+ | - |
Micrococcus sp. | D-apiose + NADH | - |
r | |
| meso-erythritol + NAD+ | 13% activity of D-apiitol oxidation | Micrococcus sp. | D-erythrose + NADH | - |
ir | |
| myo-inositol + NAD+ | 38% activity of D-apiitol oxidation | Micrococcus sp. | keto-inositol + NADH | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
reduction of D-apiose | Micrococcus sp. |
| 10 | - |
oxidation of D-apiitol | Micrococcus sp. |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Micrococcus sp. | |
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Release 2023.1 (January 2023)
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