Cloned (Comment) | Organism |
---|---|
gene YMR041c or ARA2, phylogenetic tree, expression of His6-tagged Ara2p in Escherichia coli | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
additional information | ARA2 gene disruption leads to complete loss of NAD+-specific D-arabinose dehydrogenase activity | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-arabinose | substrate inhibition of Ara2p at concentrations above 5 mM | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.78 | - |
D-arabinose | pH 8.2, 25°C, recombinant His-tagged Ara2p | Saccharomyces cerevisiae | |
4.1 | - |
NAD+ | pH 8.2, 25°C, recombinant His-tagged Ara2p | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
recombinant Ara2p, gel filtration | Saccharomyces cerevisiae |
42400 | - |
1 * 42400, recombinant Ara2p, SDS-PAGE | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-arabinose + NAD+ | Saccharomyces cerevisiae | the enzyme, mainly Ara2p not Ara1p, is involved in the biosynthesis of D-erythroascorbic acid, the five-carbon analog of ascorbic acid, from exogeneous D-arabinose | D-arabinono-1,4-lactone + NADH | - |
? | |
D-arabinose + NAD+ | Saccharomyces cerevisiae YPH250 | the enzyme, mainly Ara2p not Ara1p, is involved in the biosynthesis of D-erythroascorbic acid, the five-carbon analog of ascorbic acid, from exogeneous D-arabinose | D-arabinono-1,4-lactone + NADH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | Q04212 | ARA2; gene YMR041c or ARA2, isozyme Ara2p | - |
Saccharomyces cerevisiae YPH250 | Q04212 | ARA2; gene YMR041c or ARA2, isozyme Ara2p | - |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged Ara2p from Escherichia coli by anion exchange and nickel affinity chromatography to homogeneity | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00071 | - |
NAD+-dependent Ara2p reaction in presence of 200 mM D-arabinose, native cell extract | Saccharomyces cerevisiae |
1.8 | - |
purified recombinant His-tagged ARA2p | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-arabinose + NAD+ | the enzyme, mainly Ara2p not Ara1p, is involved in the biosynthesis of D-erythroascorbic acid, the five-carbon analog of ascorbic acid, from exogeneous D-arabinose | Saccharomyces cerevisiae | D-arabinono-1,4-lactone + NADH | - |
? | |
D-arabinose + NAD+ | preferred sugar substrate | Saccharomyces cerevisiae | D-arabinono-1,4-lactone + NADH | - |
? | |
D-arabinose + NAD+ | the enzyme, mainly Ara2p not Ara1p, is involved in the biosynthesis of D-erythroascorbic acid, the five-carbon analog of ascorbic acid, from exogeneous D-arabinose | Saccharomyces cerevisiae YPH250 | D-arabinono-1,4-lactone + NADH | - |
? | |
D-arabinose + NAD+ | preferred sugar substrate | Saccharomyces cerevisiae YPH250 | D-arabinono-1,4-lactone + NADH | - |
? | |
L-fucose + NAD+ | - |
Saccharomyces cerevisiae | ? | - |
? | |
L-fucose + NAD+ | - |
Saccharomyces cerevisiae YPH250 | ? | - |
? | |
L-galactose + NAD+ | - |
Saccharomyces cerevisiae | ? | - |
? | |
L-galactose + NAD+ | - |
Saccharomyces cerevisiae YPH250 | ? | - |
? | |
L-xylose + NAD+ | - |
Saccharomyces cerevisiae | ? | - |
? | |
additional information | ARa2p shows a broad sugar substrate specificity, but prefers D-arabinose | Saccharomyces cerevisiae | ? | - |
? | |
additional information | ARa2p shows a broad sugar substrate specificity, but prefers D-arabinose | Saccharomyces cerevisiae YPH250 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 42400, recombinant Ara2p, SDS-PAGE | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
ARA | - |
Saccharomyces cerevisiae |
Ara2p | - |
Saccharomyces cerevisiae |
NAD(+)-specific D-arabinose dehydrogenase | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | - |
- |
Saccharomyces cerevisiae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9 | 20% of maximal activity at pH 6.5 and pH 9.0, pH profile | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | Ara2p is specific for NAD+, while Ara1p utilizes NADP+ | Saccharomyces cerevisiae |