Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 2 mM | Haloferax mediterranei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.024 | - |
NADP+ | pH 8.8, 40°C | Haloferax mediterranei | |
1.2 | - |
NAD+ | pH 8.8, 40°C | Haloferax mediterranei | |
3.9 | - |
beta-D-glucose | pH 8.8, 40°C | Haloferax mediterranei |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | the activity is dependent on the concentration of NaCl or KCl in the activity buffer, being optimal at 1.3 M | Haloferax mediterranei | |
Mg2+ | besides KCl/NaCl, the activity also depends on presence of bivalent cations. Mg2+ is more effective than Mg2+ or Ni2+ | Haloferax mediterranei | |
Mn2+ | besides KCl/NaCl, the activity also depends on presence of bivalent cations. Mn2+ is less effective than Mg2+ and more effective than Ni2+ | Haloferax mediterranei | |
NaCl | the activity is dependent on the concentration of NaCl or KCl in the activity buffer, being optimal at 1.3 M | Haloferax mediterranei | |
Ni2+ | besides KCl/NaCl, the activity also depends on presence of bivalent cations. Ni2+ is more effective than Mg2+ or Mn2+ | Haloferax mediterranei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53000 | - |
2 * 53000, SDS-PAGE | Haloferax mediterranei |
89000 | - |
gel filtration | Haloferax mediterranei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haloferax mediterranei | Q977U7 | - |
- |
Haloferax mediterranei DSM 1411 | Q977U7 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Haloferax mediterranei |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
550 | - |
pH 8.8, 40°C | Haloferax mediterranei |
Storage Stability | Organism |
---|---|
4°C, stable for several months | Haloferax mediterranei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-D-glucose + NAD+ | activity with NAD+ is 26% of the activity with NADP+ | Haloferax mediterranei | ? | - |
? | |
beta-D-glucose + NAD+ | activity with NAD+ is 26% of the activity with NADP+ | Haloferax mediterranei DSM 1411 | ? | - |
? | |
beta-D-glucose + NADP+ | activity with NAD+ is 26% of the activity with NADP+. The enzyme shows also activity with D-fucose and NADP+ (at 18% compared to the activity with beta-D-glucose and NADP+), with D-galactose and NADP+ (at 13% compared to the activity with beta-D-glucose and NADP+), with D-xylose and NAD+ (at 79% compared to the activity with beta-D-glucose and NADP+), with D-xylose and NADP+ (at 88% compared to the activity with beta-D-glucose and NADP+) | Haloferax mediterranei | ? | - |
? | |
beta-D-glucose + NADP+ | activity with NAD+ is 26% of the activity with NADP+. The enzyme shows also activity with D-fucose and NADP+ (at 18% compared to the activity with beta-D-glucose and NADP+), with D-galactose and NADP+ (at 13% compared to the activity with beta-D-glucose and NADP+), with D-xylose and NAD+ (at 79% compared to the activity with beta-D-glucose and NADP+), with D-xylose and NADP+ (at 88% compared to the activity with beta-D-glucose and NADP+) | Haloferax mediterranei DSM 1411 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 53000, SDS-PAGE | Haloferax mediterranei |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
half-life: 56.8 h, in the presence of 3 M NaCl, stability decreases significantly with lower salt concentrations | Haloferax mediterranei |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | Km-value for NAD+ is 50fold higher than Km-value for NADP+ | Haloferax mediterranei | |
NADP+ | Km-value for NAD+ is 50fold higher than Km-value for NADP+ | Haloferax mediterranei |