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Literature summary for 1.1.1.12 extracted from

  • Ryu, S.; Trinh, C.T.
    Understanding functional roles of native pentose-specific transporters for activating dormant pentose metabolism in Yarrowia lipolytica (2018), Appl. Environ. Microbiol., 84, e02146-17 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.12

Protein Variants

Protein Variants Comment Organism
additional information overexpression of native pentose-specific transporters together with the rate-limiting D-xylitol and L-arabitol dehydrogenases, the dormant pentose metabolism of Yarrowia lipolytica is activated, overview. Recombinant overexpression of a heterologous L-arabitol dehydrogenase of Aspergillus oryzae (ADHAoz), in Yarrowia lipolytica strain YlSR157 using the constitutive TEF promoter, ADHAoz is chosen because it exhibits higher activity toward L-arabitol (39.2 mU/mg protein) than D-xylitol (6.54 mU/mg protein). Unlike strain YlSR102, strain YlSR157 grows faster and consumes more sugars when growing on both single arabinose and mixed pentose sugars Yarrowia lipolytica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-arabitol + NAD+ Yarrowia lipolytica
-
 L-xylulose + NADH + H+
-
 r
L-arabitol + NAD+ Yarrowia lipolytica ATCC MYA-2613
-
 L-xylulose + NADH + H+
-
 r

Organism

Organism UniProt Comment Textmining
Yarrowia lipolytica
-
-
-
Yarrowia lipolytica ATCC MYA-2613
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 in vitro enzyme activities of ARD, ADH, XLR, and XYL2 of YlSR102 growing on single xylose, single arabinose, and mixed pentose sugars, overview Yarrowia lipolytica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-arabitol + NAD+
-
 Yarrowia lipolytica L-xylulose + NADH + H+
-
 r
L-arabitol + NAD+
-
 Yarrowia lipolytica ATCC MYA-2613 L-xylulose + NADH + H+
-
 r

Synonyms

Synonyms Comment Organism
ADH
-
 Yarrowia lipolytica
NAD(P)+-dependent arabitol dehydrogenase
-
 Yarrowia lipolytica

Cofactor

Cofactor Comment Organism Structure
additional information no activity with NADP+/NADPH Yarrowia lipolytica
NAD+
-
 Yarrowia lipolytica
NADH
-
 Yarrowia lipolytica

Expression

Organism Comment Expression
Yarrowia lipolytica overexpression of native pentose-specific transporters together with the rate-limiting D-xylitol and L-arabitol dehydrogenases, the dormant pentose metabolism of Yarrowia lipolytica is activated, overview. The arabinose assimilation pathway genes are upregulated. Four ARD genes (ARD6, ARD7, ARD8, and ARD9) out of 11 putative ARD genes are upregulated in cell cultures growing on single arabinose by 2.74fold, 10.77fold, 7.21fold, and 5.86fold, respectively, compared to cells grown in single xylose up

General Information

General Information Comment Organism
metabolism Yarrowia lipolytica strain ATCC MYA-2613 has endogenous enzymes for D-xylose assimilation, but inefficient xylitol dehydrogenase causes Yarrowia lipolytica to assimilate xylose poorly. L-Arabitol dehydrogenase is the rate-limiting step responsible for poor arabinose utilization in Yarrowia lipolytica. Functional roles of native sugar-specific transporters for activating the dormant pentose metabolism in Yarrowia lipolytica, cryptic pentose metabolism and native L-arabinose assimilation pathway, overview. TRP6Yli and TRP22Yli are xylose-specific transporters in Yarrowia lipolytica. L-Arabinose is first reduced into L-arabitol by NAD(P)H-dependent arabinose reductase (ARD), which is then converted into L-xylulose by NAD(P)+-dependent arabitol dehydrogenase (ADH). L-Xylulose is then converted to D-xylitol by NAD(P)H-dependent xylulose reductase (XLR), which is further assimilated to D-xylulose-5-phosphate, a precursor for the pentose-phosphate pathway (PPP) Yarrowia lipolytica