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Literature summary for 1.1.1.121 extracted from

  • Asada, Y.; Endo, S.; Inoue, Y.; Mamiya, H.; Hara, A.; Kunishima, N.; Matsunaga, T.
    Biochemical and structural characterization of a short-chain dehydrogenase/reductase of Thermus thermophilus HB8: a hyperthermostable aldose-1-dehydrogenase with broad substrate specificity (2009), Chem. Biol. Interact., 178, 117-126.
    View publication on PubMed
Search for more literature for 1.1.1.121

Activating Compound

Activating Compound Comment Organism Structure
additional information kcat and Km values are increased by the elevation of temperature, whereas the kcat/Km values are not largely changed Thermus thermophilus HB8

Cloned(Commentary)

Cloned (Comment) Organism
into vector pET-11a and overexpressed in Escherichia coli BL21 (DE3) cells Thermus thermophilus HB8

Crystallization (Commentary)

Crystallization (Comment) Organism
by oil-microbatch method, at 1.65 A resolution. TAD follows an ordered sequential mechanism in which the coenzyme binds first Thermus thermophilus HB8

Protein Variants

Protein Variants Comment Organism
D242N mutation results in a large decrease in thermostability of TAD without dissociation into dimer or monomer Thermus thermophilus HB8
F141A mutation results in decrease in thermostability of TAD without dissociation into dimer or monomer Thermus thermophilus HB8
F249A mutation results in decrease in thermostability of TAD without dissociation into dimer or monomer Thermus thermophilus HB8

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline uncompetitive inhibition with respect to NAD+ and competitive inhibition with respect to D-galactose Thermus thermophilus HB8
2,3-benzofuran uncompetitive inhibition with respect to NAD+ and competitive inhibition with respect to D-galactose Thermus thermophilus HB8
catechol
-
 Thermus thermophilus HB8
Hexestrol uncompetitive inhibition with respect to NAD+ and competitive inhibition with respect to D-galactose Thermus thermophilus HB8
indazole
-
 Thermus thermophilus HB8
indole uncompetitive inhibition with respect to NAD+ and competitive inhibition with respect to D-galactose Thermus thermophilus HB8
additional information EDTA (1 mM) and p-chloromercuriphenylsulfonate and p-chloromercuribenzoate (0.1 mM) have no effect on the activity Thermus thermophilus HB8

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.012
-
 isatin pH 7.4, 50°C Thermus thermophilus HB8
0.14
-
 1-Acenaphthenol pH 7.4, 50°C Thermus thermophilus HB8
0.17
-
 (S)-tetralol pH 7.4, 50°C Thermus thermophilus HB8
0.25
-
 4-chromanol pH 7.4, 50°C Thermus thermophilus HB8
0.65
-
 (S)-indanol pH 7.4, 50°C Thermus thermophilus HB8
1.3
-
 (R)-indanol pH 7.4, 50°C Thermus thermophilus HB8
1.9
-
 alpha-tetralone pH 7.4, 50°C Thermus thermophilus HB8
2
-
 phenyl-1,2-propanedione pH 7.4, 50°C Thermus thermophilus HB8
2 3 D-galactosamine pH 7.4, 50°C Thermus thermophilus HB8
2.5
-
 (R)-tetralol pH 7.4, 50°C Thermus thermophilus HB8
3.7
-
 2-Cyclohexen-1-ol pH 7.4, 50°C Thermus thermophilus HB8
5.5
-
 D-fucose pH 7.4, 50°C Thermus thermophilus HB8
27
-
 cis-benzene dihydrodiol pH 7.4, 50°C Thermus thermophilus HB8
33
-
 2-deoxy-D-galactose pH 7.4, 50°C Thermus thermophilus HB8
78
-
 D-galactose pH 7.4, 50°C Thermus thermophilus HB8
131
-
 D-talose pH 7.4, 50°C Thermus thermophilus HB8
139
-
 D-glucosamine pH 7.4, 50°C Thermus thermophilus HB8
162
-
 L-fucose pH 7.4, 50°C Thermus thermophilus HB8
178
-
 6-deoxy-D-glucose pH 7.4, 50°C Thermus thermophilus HB8
250
-
 cellobiose pH 7.4, 50°C Thermus thermophilus HB8
262
-
 L-arabinose pH 7.4, 50°C Thermus thermophilus HB8
300
-
 2-deoxy-D-glucose pH 7.4, 50°C Thermus thermophilus HB8
580
-
 D-glucose pH 7.4, 50°C Thermus thermophilus HB8
781
-
 lactose pH 7.4, 50°C Thermus thermophilus HB8
841
-
 D-xylose pH 7.4, 50°C Thermus thermophilus HB8

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
 4 * 27000, SDS-PAGE Thermus thermophilus HB8
100000
-
 gel filtration Thermus thermophilus HB8

Organism

Organism UniProt Comment Textmining
Thermus thermophilus HB8 Q5SLC4
-
-

Purification (Commentary)

Purification (Comment) Organism
by sonication, heating at 70°C for 10 min, ion-exchange chromatography, gel filtration and ultrafiltration Thermus thermophilus HB8

Storage Stability

Storage Stability Organism
4°C Thermus thermophilus HB8

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-indanol + NAD+
-
 Thermus thermophilus HB8 ? + NADH + H+
-
 ?
(R)-tetralol + NAD+
-
 Thermus thermophilus HB8 ? + NADH + H+
-
 ?
(S)-(+)-1,2,3,4-tetrahydro-1-naphthol + NAD+
-
 Thermus thermophilus HB8 ? + NADH + H+
-
 ?
(S)-(+)-1-indanol + NAD+
-
 Thermus thermophilus HB8 ? + NADH + H+
-
 ?
(S)-indanol + NAD+
-
 Thermus thermophilus HB8 ? + NADH + H+
-
 ?
(S)-tetralol + NAD+
-
 Thermus thermophilus HB8 ? + NADH + H+
-
 ?
1-acenaphthenol + NAD+
-
 Thermus thermophilus HB8 ? + NADH + H+
-
 ?
2-cyclohexen-1-ol + NAD+
-
 Thermus thermophilus HB8 ? + NADH + H+
-
 ?
2-deoxy-D-galactose + NAD+
-
 Thermus thermophilus HB8 ? + NADH + H+
-
 ?
2-deoxy-D-glucose + NAD+
-
 Thermus thermophilus HB8 ? + NADH + H+
-
 ?
4-chromanol + NAD+
-
 Thermus thermophilus HB8 ? + NADH + H+
-
 ?
6-deoxy-D-glucose
-
 Thermus thermophilus HB8 ? + NADH + H+
-
 ?
alpha-tetralone + NADH + H+
-
 Thermus thermophilus HB8 ? + NAD+
-
 ?
cellobiose + NAD+
-
 Thermus thermophilus HB8 ? + NADH + H+
-
 ?
cis-benzene dihydrodiol + NAD+
-
 Thermus thermophilus HB8 ? + NADH + H+
-
 ?
D-fucose + NAD+
-
 Thermus thermophilus HB8 D-fucono-delta-lactone + NADH + H+
-
 ?
D-galactosamine + NAD+
-
 Thermus thermophilus HB8 ? + NADH + H+
-
 ?
D-galactose + NAD+
-
 Thermus thermophilus HB8 D-galactono-gamma-lactone + NADH + H+
-
 ?
D-glucosamine + NAD+
-
 Thermus thermophilus HB8 ? + NADH + H+
-
 ?
D-glucose + NAD+
-
 Thermus thermophilus HB8 D-glucono-delta-lactone + NADH + H+
-
 ?
D-talose + NAD+
-
 Thermus thermophilus HB8 ? + NADH + H+
-
 ?
D-xylose + NAD+
-
 Thermus thermophilus HB8 ? + NADH + H+
-
 ?
glucono-delta-lactone + NADH + H+
-
 Thermus thermophilus HB8 ? + NAD+
-
 ?
isatin + NADH + H+
-
 Thermus thermophilus HB8 ? + NAD+
-
 ?
L-arabinose + NAD+
-
 Thermus thermophilus HB8 ? + NADH + H+
-
 ?
L-fucose + NAD+
-
 Thermus thermophilus HB8 ? + NADH + H+
-
 ?
lactose + NAD+
-
 Thermus thermophilus HB8 ? + NADH + H+
-
 ?
additional information TAD possesses the catalytic triad Ser136, Tyr149, and Lys153. No activity with L-xylose, D-ribose and 2-deoxy-D-ribose. TAD does not oxidize ketoses (D-fructose and D-sorbose), sugar alcohols (D-sorbitol, galactitol, inositol, L-arabitol and xylitol), uronic acids (D-galacturonic acid and D-glucuronic acid), aldonic acids (L-gulonic acid and 2-keto-L-gulonic acid), aromatic alcohols (benzyl alcohol and catechol), aliphatic alcohols (1-nonanol, 1-decanol, 3-hydroxybutyric acid and 2,3-butanediol), and hydroxysteroids (androsterone, testosterone, 20alpha/beta-hydroxyprogesterone, cholic acid, cortisol and corticosterone). No significant activity ketones (4-nitroacetophenone, camphorquinone, 2,3-hexanedione and diacetyl) and aldehydes (4-nitrobenzaldehyde, pyridine-3-aldehyde, 1-nonanal and citral) Thermus thermophilus HB8 ?
-
 ?
phenyl-1,2-propanedione + NADH + H+
-
 Thermus thermophilus HB8 ? + NAD+
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 27000, SDS-PAGE Thermus thermophilus HB8

Synonyms

Synonyms Comment Organism
TAD
-
 Thermus thermophilus HB8

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
80 85
-
 Thermus thermophilus HB8

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
85
-
 approximately 40% of the activity is inactivated by the incubation of the enzyme solution (0.3 mg/ml in 10 mM potassium phosphate, pH 7.0) for 15 min at 95°C Thermus thermophilus HB8

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.075
-
 (R)-tetralol pH 7.4, 50°C Thermus thermophilus HB8
0.1067
-
 L-fucose pH 7.4, 50°C Thermus thermophilus HB8
0.167
-
 (R)-indanol pH 7.4, 50°C Thermus thermophilus HB8
0.3117
-
 D-galactosamine pH 7.4, 50°C Thermus thermophilus HB8
0.423
-
 D-xylose pH 7.4, 50°C Thermus thermophilus HB8
0.6217
-
 D-glucosamine pH 7.4, 50°C Thermus thermophilus HB8
3.017
-
 D-talose pH 7.4, 50°C Thermus thermophilus HB8
3.5
-
 2-Cyclohexen-1-ol pH 7.4, 50°C Thermus thermophilus HB8
3.85
-
 D-glucose pH 7.4, 50°C Thermus thermophilus HB8
3.9
-
 alpha-tetralone pH 7.4, 50°C Thermus thermophilus HB8
4.55
-
 L-arabinose pH 7.4, 50°C Thermus thermophilus HB8
5.767
-
 cellobiose pH 7.4, 50°C Thermus thermophilus HB8
6.35
-
 6-deoxy-D-glucose pH 7.4, 50°C Thermus thermophilus HB8
6.93
-
 2-deoxy-D-glucose pH 7.4, 50°C Thermus thermophilus HB8
7.383
-
 cis-benzene dihydrodiol pH 7.4, 50°C Thermus thermophilus HB8
8.25
-
 D-galactose pH 7.4, 50°C Thermus thermophilus HB8
8.73
-
 D-fucose pH 7.4, 50°C Thermus thermophilus HB8
9.23
-
 lactose pH 7.4, 50°C Thermus thermophilus HB8
12.8
-
 2-deoxy-D-galactose pH 7.4, 50°C Thermus thermophilus HB8
16.83
-
 phenyl-1,2-propanedione pH 7.4, 50°C Thermus thermophilus HB8
40.3
-
 4-chromanol pH 7.4, 50°C Thermus thermophilus HB8
138.7
-
 (S)-indanol pH 7.4, 50°C Thermus thermophilus HB8
161.7
-
 (S)-tetralol pH 7.4, 50°C Thermus thermophilus HB8
433.3
-
 1-Acenaphthenol pH 7.4, 50°C Thermus thermophilus HB8
981.7
-
 isatin pH 7.4, 50°C Thermus thermophilus HB8

pH Stability

pH Stability pH Stability Maximum Comment Organism
2 13
-
 Thermus thermophilus HB8

Cofactor

Cofactor Comment Organism Structure
additional information TAD does not exhibit dehydrogenase activity with NADP+ as the coenzyme Thermus thermophilus HB8
NAD+
-
 Thermus thermophilus HB8

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.013
-
 Hexestrol
-
 Thermus thermophilus HB8
0.025
-
 1,10-phenanthroline
-
 Thermus thermophilus HB8
0.042
-
 2,3-benzofuran
-
 Thermus thermophilus HB8
0.48
-
 indole
-
 Thermus thermophilus HB8

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.063
-
-
 Thermus thermophilus HB8 Hexestrol
0.18
-
-
 Thermus thermophilus HB8 1,10-phenanthroline
0.2
-
-
 Thermus thermophilus HB8 2,3-benzofuran
0.48
-
-
 Thermus thermophilus HB8 indole
1.2
-
-
 Thermus thermophilus HB8 catechol
1.9
-
-
 Thermus thermophilus HB8 indazole

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0005
-
 D-xylose pH 7.4, 50°C Thermus thermophilus HB8
0.00067
-
 L-fucose pH 7.4, 50°C Thermus thermophilus HB8
0.0045
-
 D-glucosamine pH 7.4, 50°C Thermus thermophilus HB8
0.0067
-
 D-glucose pH 7.4, 50°C Thermus thermophilus HB8
0.012
-
 lactose pH 7.4, 50°C Thermus thermophilus HB8
0.014
-
 D-galactosamine pH 7.4, 50°C Thermus thermophilus HB8
0.017
-
 L-arabinose pH 7.4, 50°C Thermus thermophilus HB8
0.023
-
 2-deoxy-D-glucose pH 7.4, 50°C Thermus thermophilus HB8
0.023
-
 D-talose pH 7.4, 50°C Thermus thermophilus HB8
0.023
-
 cellobiose pH 7.4, 50°C Thermus thermophilus HB8
0.03
-
 (R)-tetralol pH 7.4, 50°C Thermus thermophilus HB8
0.035
-
 6-deoxy-D-glucose pH 7.4, 50°C Thermus thermophilus HB8
0.11
-
 D-galactose pH 7.4, 50°C Thermus thermophilus HB8
0.13
-
 (R)-indanol pH 7.4, 50°C Thermus thermophilus HB8
0.27
-
 cis-benzene dihydrodiol pH 7.4, 50°C Thermus thermophilus HB8
0.38
-
 2-deoxy-D-galactose pH 7.4, 50°C Thermus thermophilus HB8
0.95
-
 2-Cyclohexen-1-ol pH 7.4, 50°C Thermus thermophilus HB8
1.58
-
 D-fucose pH 7.4, 50°C Thermus thermophilus HB8
61.7
-
 4-chromanol pH 7.4, 50°C Thermus thermophilus HB8
216.7
-
 (S)-indanol pH 7.4, 50°C Thermus thermophilus HB8
950
-
 (S)-tetralol pH 7.4, 50°C Thermus thermophilus HB8
3000
-
 1-Acenaphthenol pH 7.4, 50°C Thermus thermophilus HB8