Purification and characterization of L-fucose dehydrogenase from Agrobacterium tumefaciens K-28

Data extracted from this reference:

Inhibitors

Commentary

Organism

Structure

Cu2+

80% inhibition

Agrobacterium tumefaciens

Hg2+

complete inhibition

Agrobacterium tumefaciens

p-chloromercuribenzoate

90% inhibition

Agrobacterium tumefaciens

Zn2+

40% inhibition

Agrobacterium tumefaciens

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

0.6

-

NADP+

-

Agrobacterium tumefaciens

1.89

-

NAD+

-

Agrobacterium tumefaciens

11.1

-

D-galactose

-

Agrobacterium tumefaciens

12.8

-

6-deoxy-L-galactose

-

Agrobacterium tumefaciens

34.4

-

D-arabinose

-

Agrobacterium tumefaciens

Natural Substrates

Organism

Commentary (Nat. Sub.)

Natural Products

Commentary (Nat. Pro.)

Organism (Nat. Pro.)

Reversibility

ID

6-deoxy-L-galactose + NAD+

Agrobacterium tumefaciens

i.e. L-fucose

6-deoxy-L-galactono-1,5-lactone + NADH

-

Agrobacterium tumefaciens

?

6-deoxy-L-galactose + NAD+

Agrobacterium tumefaciens K-28

i.e. L-fucose

6-deoxy-L-galactono-1,5-lactone + NADH

-

Agrobacterium tumefaciens K-28

?

D-arabinose + NAD+

Agrobacterium tumefaciens

74.5% of the activity compared to L-fucose

D-arabinono-1,4-lactone + NADH

-

Agrobacterium tumefaciens

?

D-arabinose + NAD+

Agrobacterium tumefaciens K-28

74.5% of the activity compared to L-fucose

D-arabinono-1,4-lactone + NADH

-

Agrobacterium tumefaciens K-28

?

L-galactose + NAD+

Agrobacterium tumefaciens

59.6% of the activity compared to L-fucose

L-galactono-1,5-lactone + NADH

-

Agrobacterium tumefaciens

?

L-galactose + NAD+

Agrobacterium tumefaciens K-28

59.6% of the activity compared to L-fucose

L-galactono-1,5-lactone + NADH

-

Agrobacterium tumefaciens K-28

?

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

Substrate Product ID

6-deoxy-L-galactose + NAD+

i.e. L-fucose

285798

Agrobacterium tumefaciens

6-deoxy-L-galactono-1,5-lactone + NADH

-

285798

Agrobacterium tumefaciens

?

6-deoxy-L-galactose + NAD+

i.e. L-fucose

285798

Agrobacterium tumefaciens K-28

6-deoxy-L-galactono-1,5-lactone + NADH

-

285798

Agrobacterium tumefaciens K-28

?

D-arabinose + NAD+

74.5% of the activity compared to L-fucose

285798

Agrobacterium tumefaciens

D-arabinono-1,4-lactone + NADH

-

285798

Agrobacterium tumefaciens

?

D-arabinose + NAD+

74.5% of the activity compared to L-fucose

285798

Agrobacterium tumefaciens K-28

D-arabinono-1,4-lactone + NADH

-

285798

Agrobacterium tumefaciens K-28

?

D-galactose + NAD+

-

285798

Agrobacterium tumefaciens

D-galactono-1,5-lactone + NADH + H+

-

-

-

r

D-galactose + NAD+

-

285798

Agrobacterium tumefaciens K-28

D-galactono-1,5-lactone + NADH + H+

-

-

-

r

fuconolactone + NADPH + H+

-

285798

Agrobacterium tumefaciens

L-fucose + NADP+

-

-

-

?

fuconolactone + NADPH + H+

-

285798

Agrobacterium tumefaciens K-28

L-fucose + NADP+

-

-

-

?

L-galactose + NAD+

59.6% of the activity compared to L-fucose

285798

Agrobacterium tumefaciens

L-galactono-1,5-lactone + NADH

-

285798

Agrobacterium tumefaciens

?

L-galactose + NAD+

59.6% of the activity compared to L-fucose

285798

Agrobacterium tumefaciens K-28

L-galactono-1,5-lactone + NADH

-

285798

Agrobacterium tumefaciens K-28

?

Cofactor

Commentary

Organism

Structure

NAD+

also able to use NADP+

Agrobacterium tumefaciens

Cofactor

Commentary

Organism

Structure

NAD+

also able to use NADP+

Agrobacterium tumefaciens

Inhibitors

Commentary

Organism

Structure

Cu2+

80% inhibition

Agrobacterium tumefaciens

Hg2+

complete inhibition

Agrobacterium tumefaciens

p-chloromercuribenzoate

90% inhibition

Agrobacterium tumefaciens

Zn2+

40% inhibition

Agrobacterium tumefaciens

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

0.6

-

NADP+

-

Agrobacterium tumefaciens

1.89

-

NAD+

-

Agrobacterium tumefaciens

11.1

-

D-galactose

-

Agrobacterium tumefaciens

12.8

-

6-deoxy-L-galactose

-

Agrobacterium tumefaciens

34.4

-

D-arabinose

-

Agrobacterium tumefaciens

Natural Substrates

Organism

Commentary (Nat. Sub.)

Natural Products

Commentary (Nat. Pro.)

Organism (Nat. Pro.)

Reversibility

ID

6-deoxy-L-galactose + NAD+

Agrobacterium tumefaciens

i.e. L-fucose

6-deoxy-L-galactono-1,5-lactone + NADH

-

Agrobacterium tumefaciens

?

6-deoxy-L-galactose + NAD+

Agrobacterium tumefaciens K-28

i.e. L-fucose

6-deoxy-L-galactono-1,5-lactone + NADH

-

Agrobacterium tumefaciens K-28

?

D-arabinose + NAD+

Agrobacterium tumefaciens

74.5% of the activity compared to L-fucose

D-arabinono-1,4-lactone + NADH

-

Agrobacterium tumefaciens

?

D-arabinose + NAD+

Agrobacterium tumefaciens K-28

74.5% of the activity compared to L-fucose

D-arabinono-1,4-lactone + NADH

-

Agrobacterium tumefaciens K-28

?

L-galactose + NAD+

Agrobacterium tumefaciens

59.6% of the activity compared to L-fucose

L-galactono-1,5-lactone + NADH

-

Agrobacterium tumefaciens

?

L-galactose + NAD+

Agrobacterium tumefaciens K-28

59.6% of the activity compared to L-fucose

L-galactono-1,5-lactone + NADH

-

Agrobacterium tumefaciens K-28

?

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

ID

6-deoxy-L-galactose + NAD+

i.e. L-fucose

285798

Agrobacterium tumefaciens

6-deoxy-L-galactono-1,5-lactone + NADH

-

285798

Agrobacterium tumefaciens

?

6-deoxy-L-galactose + NAD+

i.e. L-fucose

285798

Agrobacterium tumefaciens K-28

6-deoxy-L-galactono-1,5-lactone + NADH

-

285798

Agrobacterium tumefaciens K-28

?

D-arabinose + NAD+

74.5% of the activity compared to L-fucose

285798

Agrobacterium tumefaciens

D-arabinono-1,4-lactone + NADH

-

285798

Agrobacterium tumefaciens

?

D-arabinose + NAD+

74.5% of the activity compared to L-fucose

285798

Agrobacterium tumefaciens K-28

D-arabinono-1,4-lactone + NADH

-

285798

Agrobacterium tumefaciens K-28

?

D-galactose + NAD+

-

285798

Agrobacterium tumefaciens

D-galactono-1,5-lactone + NADH + H+

-

-

-

r

D-galactose + NAD+

-

285798

Agrobacterium tumefaciens K-28

D-galactono-1,5-lactone + NADH + H+

-

-

-

r

fuconolactone + NADPH + H+

-

285798

Agrobacterium tumefaciens

L-fucose + NADP+

-

-

-

?

fuconolactone + NADPH + H+

-

285798

Agrobacterium tumefaciens K-28

L-fucose + NADP+

-

-

-

?

L-galactose + NAD+

59.6% of the activity compared to L-fucose

285798

Agrobacterium tumefaciens

L-galactono-1,5-lactone + NADH

-

285798

Agrobacterium tumefaciens

?

L-galactose + NAD+

59.6% of the activity compared to L-fucose

285798

Agrobacterium tumefaciens K-28

L-galactono-1,5-lactone + NADH

-

285798

Agrobacterium tumefaciens K-28

?

No.

1st author

Pub Med

title

organims

journal

volume

pages

year

Activating Compound

Application

Cloned(Commentary)

Crystallization (Commentary)

Engineering

General Stability

Inhibitors

KM Value [mM]

Localization

Metals/Ions

Molecular Weight [Da]

Natural Substrates/ Products (Substrates)

Organic Solvent Stability

Organism

Oxidation Stability

Posttranslational Modification

Purification (Commentary)

Reaction

Renatured (Commentary)

Source Tissue

Specific Activity [micromol/min/mg]

Storage Stability

Substrates and Products (Substrate)

Subunits

Synonyms

Temperature Optimum [°C]

Temperature Range [°C]

Temperature Stability [°C]

Turnover Number [1/s]

pH Optimum

pH Range

pH Stability

Cofactor

Ki Value [mM]

pI Value

IC50 Value

Activating Compound (protein specific)

Application (protein specific)

Cloned(Commentary) (protein specific)

Cofactor (protein specific)

Crystallization (Commentary) (protein specific)

Engineering (protein specific)

General Stability (protein specific)

IC50 Value (protein specific)

Inhibitors (protein specific)

Ki Value [mM] (protein specific)

KM Value [mM] (protein specific)

Localization (protein specific)

Metals/Ions (protein specific)

Molecular Weight [Da] (protein specific)

Natural Substrates/ Products (Substrates) (protein specific)

Organic Solvent Stability (protein specific)

Oxidation Stability (protein specific)

Posttranslational Modification (protein specific)

Purification (Commentary) (protein specific)

Renatured (Commentary) (protein specific)

Source Tissue (protein specific)

Specific Activity [micromol/min/mg] (protein specific)

Storage Stability (protein specific)

Substrates and Products (Substrate) (protein specific)

Subunits (protein specific)

Temperature Optimum [°C] (protein specific)

Temperature Range [°C] (protein specific)

Temperature Stability [°C] (protein specific)

Turnover Number [1/s] (protein specific)

pH Optimum (protein specific)

pH Range (protein specific)

pH Stability (protein specific)

pI Value (protein specific)

Expression

General Information

General Information (protein specific)

Expression (protein specific)

KCat/KM [mM/s]

KCat/KM [mM/s] (protein specific)

741316

Ohshiro

Production and characterizatio ...

Acinetobacter sp., Acinetobacter sp. SA-134

Prep. Biochem. Biotechnol.

44

382-391

2014

-

-

-

-

-

-

6

3

-

-

2

2

-

8

-

-

1

-

-

-

2

-

11

1

1

1

-

1

-

1

-

1

2

-

-

-

-

-

-

2

-

-

-

-

6

-

3

-

-

2

2

-

-

-

1

-

-

2

-

11

1

1

-

1

-

1

-

1

-

-

-

-

-

-

-

681445

Brouns

Crystal structure and biochemi ...

Saccharolobus solfataricus, Saccharolobus solfataricus P2

J. Mol. Biol.

371

1249-1260

2007

-

-

-

1

-

-

-

-

-

1

-

-

-

3

-

-

-

-

-

-

-

-

2

1

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

1

1

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

2

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

719837

Brouns

Identification of the missing ...

Saccharolobus solfataricus, Saccharolobus solfataricus P2

J. Biol. Chem.

281

27378-27388

2006

-

-

1

-

-

-

-

-

-

1

2

-

-

3

-

-

-

-

-

-

-

-

2

1

1

1

-

2

-

1

-

-

1

-

-

-

-

-

1

1

-

-

-

-

-

-

-

-

1

2

-

-

-

-

-

-

-

-

-

2

1

1

-

2

-

1

-

-

-

-

-

-

-

-

-

655086

Bakke

-

Improvement in thermal stabili ...

Pseudomonas sp., Pseudomonas sp. No. 1143

Biotechnol. Lett.

24

1075-1078

2002

-

-

-

-

-

1

-

-

-

-

-

-

-

2

-

-

-

-

-

-

-

-

2

-

1

-

-

2

-

-

-

-

1

-

-

-

-

-

-

1

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

2

-

-

-

2

-

-

-

-

-

-

-

-

-

-

-

285798

Itoh

-

Purification and characterizat ...

Agrobacterium tumefaciens, Agrobacterium tumefaciens K-28

J. Ferment. Bioeng.

77

100-102

1994

-

-

-

-

-

-

4

5

-

-

1

6

-

2

-

-

1

-

-

1

2

-

10

-

-

1

-

1

-

1

-

1

1

-

-

-

-

-

-

1

-

-

-

-

4

-

5

-

-

1

6

-

-

-

1

-

1

2

-

10

-

1

-

1

-

1

-

1

-

-

-

-

-

-

-

285799

Yamamoto-Otake

Cloning and sequencing of the ...

Pseudomonas sp.

Biosci. Biotechnol. Biochem.

58

2281-2282

1994

-

-

1

-

-

-

-

-

-

-

-

1

-

9

-

-

-

-

-

-

-

-

1

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

285800

Tsuji

Purification and some properti ...

Agrobacterium tumefaciens

Biochim. Biophys. Acta

1117

167-173

1992

-

1

-

-

-

1

4

4

-

-

3

3

-

10

-

-

1

-

-

1

1

1

4

1

-

-

-

1

-

-

1

1

2

-

-

-

-

1

-

2

-

-

1

-

4

-

4

-

-

3

3

-

-

-

1

-

1

1

1

4

1

-

-

1

-

-

1

1

-

-

-

-

-

-

-

285801

Guimaraes

-

Purification and general prope ...

Aureobasidium pullulans

Arq. Biol. Tecnol. (Curitiba)

32

575-587

1989

-

-

-

-

-

-

2

4

-

-

1

3

-

1

-

-

1

-

-

1

-

1

3

1

-

-

-

1

-

1

-

-

1

-

-

-

-

-

-

1

-

-

-

-

2

-

4

-

-

1

3

-

-

-

1

-

1

-

1

3

1

-

-

1

-

1

-

-

-

-

-

-

-

-

-

285802

Sasajima

Oxidation of L-glucose by a ps ...

Trinickia caryophylli

Biochim. Biophys. Acta

571

120-126

1979

-

-

-

-

-

1

4

4

-

-

1

7

-

1

-

-

1

-

-

1

1

1

7

-

-

-

-

-

-

1

-

-

2

-

-

-

-

-

-

2

-

-

1

-

4

-

4

-

-

1

7

-

-

-

1

-

1

1

1

7

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

285805

Endo

Isolation and characterization ...

Oryctolagus cuniculus

J. Biochem.

86

1559-1565

1979

-

-

-

-

-

-

4

3

-

-

1

4

-

6

-

-

1

-

-

3

1

1

4

-

-

1

-

1

-

1

-

1

1

-

-

-

-

-

-

1

-

-

-

-

4

-

3

-

-

1

4

-

-

-

1

-

3

1

1

4

-

1

-

1

-

1

-

1

-

-

-

-

-

-

-

285803

Mobley

The physical properties of NAD ...

Ovis aries aries

Arch. Biochem. Biophys.

186

184-188

1978

5

-

-

-

-

1

3

1

-

-

3

2

-

3

-

-

1

-

-

2

-

1

2

1

-

-

-

2

-

-

-

-

1

-

-

-

5

-

-

1

-

-

1

-

3

-

1

-

-

3

2

-

-

-

1

-

2

-

1

2

1

-

-

2

-

-

-

-

-

-

-

-

-

-

-

285804

Mobley

L-Fucose dehydrogenase from sh ...

Ovis aries aries

Methods Enzymol.

41B

173-177

1975

-

-

-

-

-

1

-

6

-

-

1

4

-

3

-

-

1

-

-

2

-

1

4

-

-

-

-

1

-

1

-

-

1

-

-

-

-

-

-

1

-

-

1

-

-

-

6

-

-

1

4

-

-

-

1

-

2

-

1

4

-

-

-

1

-

1

-

-

-

-

-

-

-

-

-

285806

Schacter

Isolation of diphosphopyridine ...

Sus scrofa

J. Biol. Chem.

244

4785-4792

1969

1

-

-

-

-

-

4

7

-

-

-

6

-

3

-

-

1

-

-

2

1

-

7

-

-

-

-

-

-

1

-

-

1

-

-

-

1

-

-

1

-

-

-

-

4

-

7

-

-

-

6

-

-

-

1

-

2

1

-

7

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-