BRENDA - Reference to 1.1.1.122; Id = 285803

KNO3

activation at 1-1.3 M, optimal pH shifts to 9-9.5

Ovis aries aries

NaBr

activation at 1-1.3 M, optimal pH shifts to 9-9.5

Ovis aries aries

NaCl

activation at 1-1.3 M, optimal pH shifts to 9-9.5

Ovis aries aries

NaNO3

activation at 1-1.3 M, optimal pH shifts to 9-9.5

Ovis aries aries

Tris-HCl

activation at 1-1.3 M, optimal pH shifts to 9-9.5

Ovis aries aries

NAD+ stabilizes

Ovis aries aries

ADP

slight inhibition

Ovis aries aries

ATP

slight inhibition

Ovis aries aries

NADH

Ki: 0.04 mM

Ovis aries aries

0.2

-

L-galactose

-

Ovis aries aries

30000

-

x * 30000, SDS-PAGE, x * 35000, guanidinium-HCl gel filtration

Ovis aries aries

35000

-

x * 30000, SDS-PAGE, x * 35000, guanidinium-HCl gel filtration

Ovis aries aries

82000

123000

sedimentation equilibrium analysis: 123000 Da, gel filtration: 82000 Da

Ovis aries aries

6-deoxy-L-galactose + NAD+

Ovis aries aries

i.e. L-fucose

6-deoxy-L-galactono-1,5-lactone + NADH

-

Ovis aries aries

?

L-galactose + NAD+

Ovis aries aries

-

L-galactono-1,5-lactone + NADH

-

Ovis aries aries

?

Ovis aries aries

-

sheep

-

to homogeneity, chromatography techniques

Ovis aries aries

liver

-

Ovis aries aries

-

4°C, pH 7.2, half-life: 39 days

Ovis aries aries

6-deoxy-L-galactose + NAD+

i.e. L-fucose

285803

Ovis aries aries

6-deoxy-L-galactono-1,5-lactone + NADH

-

285803

Ovis aries aries

?

L-galactose + NAD+

-

285803

Ovis aries aries

L-galactono-1,5-lactone + NADH

-

285803

Ovis aries aries

?

x * 30000, SDS-PAGE, x * 35000, guanidinium-HCl gel filtration

Ovis aries aries

25

-

at pH 7.2: half-life: 1.8 days

Ovis aries aries

60

-

at pH 7.2, half-life: 25 min without added NAD+, half-life: 35 min including added NAD+, at pH 8.5: activity more rapidly lost

Ovis aries aries

NAD+

-

Ovis aries aries

KNO3

activation at 1-1.3 M, optimal pH shifts to 9-9.5

Ovis aries aries

NaBr

activation at 1-1.3 M, optimal pH shifts to 9-9.5

Ovis aries aries

NaCl

activation at 1-1.3 M, optimal pH shifts to 9-9.5

Ovis aries aries

NaNO3

activation at 1-1.3 M, optimal pH shifts to 9-9.5

Ovis aries aries

Tris-HCl

activation at 1-1.3 M, optimal pH shifts to 9-9.5

Ovis aries aries

NAD+

-

Ovis aries aries

NAD+ stabilizes

Ovis aries aries

ADP

slight inhibition

Ovis aries aries

ATP

slight inhibition

Ovis aries aries

NADH

Ki: 0.04 mM

Ovis aries aries

0.2

-

L-galactose

-

Ovis aries aries

30000

-

x * 30000, SDS-PAGE, x * 35000, guanidinium-HCl gel filtration

Ovis aries aries

35000

-

x * 30000, SDS-PAGE, x * 35000, guanidinium-HCl gel filtration

Ovis aries aries

82000

123000

sedimentation equilibrium analysis: 123000 Da, gel filtration: 82000 Da

Ovis aries aries

6-deoxy-L-galactose + NAD+

Ovis aries aries

i.e. L-fucose

6-deoxy-L-galactono-1,5-lactone + NADH

-

Ovis aries aries

?

L-galactose + NAD+

Ovis aries aries

-

L-galactono-1,5-lactone + NADH

-

Ovis aries aries

?

to homogeneity, chromatography techniques

Ovis aries aries

liver

-

Ovis aries aries

-

4°C, pH 7.2, half-life: 39 days

Ovis aries aries

6-deoxy-L-galactose + NAD+

i.e. L-fucose

285803

Ovis aries aries

6-deoxy-L-galactono-1,5-lactone + NADH

-

285803

Ovis aries aries

?

L-galactose + NAD+

-

285803

Ovis aries aries

L-galactono-1,5-lactone + NADH

-

285803

Ovis aries aries

?

x * 30000, SDS-PAGE, x * 35000, guanidinium-HCl gel filtration

Ovis aries aries

25

-

at pH 7.2: half-life: 1.8 days

Ovis aries aries

60

-

at pH 7.2, half-life: 25 min without added NAD+, half-life: 35 min including added NAD+, at pH 8.5: activity more rapidly lost

Ovis aries aries

741316

Ohshiro

Production and characterizatio ...

Acinetobacter sp., Acinetobacter sp. SA-134

Prep. Biochem. Biotechnol.

44

382-391

2014

-

6

3

-

2

-

4

-

1

-

2

-

11

1

-

1

-

1

-

1

2

-

2

-

6

-

3

-

2

-

1

-

2

-

11

1

-

1

-

1

-

1

-

681445

Brouns

Crystal structure and biochemi ...

Saccharolobus solfataricus, Saccharolobus solfataricus P2

J. Mol. Biol.

371

1249-1260

2007

-

1

-

1

-

3

-

2

1

-

1

-

1

-

1

-

2

1

-

719837

Brouns

Identification of the missing ...

Saccharolobus solfataricus, Saccharolobus solfataricus P2

J. Biol. Chem.

281

27378-27388

2006

-

1

-

1

2

-

3

-

2

1

-

2

-

1

-

1

-

1

-

1

2

-

2

1

-

2

-

1

-

655086

Bakke

-

Improvement in thermal stabili ...

Pseudomonas sp., Pseudomonas sp. No. 1143

Biotechnol. Lett.

24

1075-1078

2002

-

1

-

2

-

2

-

1

-

2

-

1

-

1

-

1

-

2

-

2

-

285798

Itoh

-

Purification and characterizat ...

Agrobacterium tumefaciens, Agrobacterium tumefaciens K-28

J. Ferment. Bioeng.

77

100-102

1994

-

4

5

-

1

6

-

2

-

1

-

1

2

-

8

-

1

-

1

-

1

-

1

-

1

-

4

-

5

-

1

6

-

1

-

1

2

-

8

-

1

-

1

-

1

-

1

-

285799

Yamamoto-Otake

Cloning and sequencing of the ...

Pseudomonas sp.

Biosci. Biotechnol. Biochem.

58

2281-2282

1994

-

1

-

1

-

3

-

1

-

1

-

1

-

1

-

1

-

285800

Tsuji

Purification and some properti ...

Agrobacterium tumefaciens

Biochim. Biophys. Acta

1117

167-173

1992

-

1

-

1

4

-

3

-

4

-

1

-

1

4

1

-

1

-

1

2

-

1

-

2

-

1

-

4

-

4

-

3

-

1

-

1

4

1

-

1

-

1

-

285801

Guimaraes

-

Purification and general prope ...

Aureobasidium pullulans

Arq. Biol. Tecnol. (Curitiba)

32

575-587

1989

-

2

4

-

1

3

-

1

-

1

-

1

-

1

3

1

-

1

-

1

-

1

-

1

-

2

-

4

-

1

3

-

1

-

1

-

1

3

1

-

1

-

1

-

285802

Sasajima

Oxidation of L-glucose by a ps ...

Trinickia caryophylli

Biochim. Biophys. Acta

571

120-126

1979

-

1

4

-

1

7

-

1

-

1

-

1

7

-

1

-

2

-

2

-

1

-

4

-

4

-

1

7

-

1

-

1

7

-

1

-

285805

Endo

Isolation and characterization ...

Oryctolagus cuniculus

J. Biochem.

86

1559-1565

1979

-

4

3

-

1

4

-

2

-

1

-

2

1

4

-

1

-

1

-

1

-

1

-

1

-

4

-

3

-

1

4

-

1

-

2

1

4

-

1

-

1

-

1

-

1

-

285803

Mobley

The physical properties of NAD ...

Ovis aries aries

Arch. Biochem. Biophys.

186

184-188

1978

5

-

1

3

1

-

3

2

-

2

-

1

-

2

-

1

2

1

-

2

-

1

-

5

-

1

-

1

-

3

-

1

-

3

2

-

1

-

2

-

1

2

1

-

2

-

285804

Mobley

L-Fucose dehydrogenase from sh ...

Ovis aries aries

Methods Enzymol.

41B

173-177

1975

-

1

-

6

-

1

4

-

2

-

1

-

2

-

1

4

-

1

-

1

-

1

-

1

-

1

-

6

-

1

4

-

1

-

2

-

1

4

-

1

-

1

-

285806

Schacter

Isolation of diphosphopyridine ...

Sus scrofa

J. Biol. Chem.

244

4785-4792

1969

1

-

4

7

-

6

-

2

-

1

-

2

1

-

6

-

1

-

1

-

1

-

1

-

4

-

7

-

6

-

1

-

2

1

-

6

-

1

-

The physical properties of NAD-dependent L-fucose dehydrogenase from sheep liver

Data extracted from this reference: