Production and characterization of L-fucose dehydrogenase from newly isolated Acinetobacter sp. strain SA-134
Ohshiro, T.; Morita, N.; Prep. Biochem. Biotechnol. 44, 382-391 (2014) 
Data extracted from this reference:
Inhibitors
Inhibitors
Commentary
Organism
Structure
Ag+
complete inhibition at 1 mM
Acinetobacter sp.
Co2+
complete inhibition at 1 mM
Acinetobacter sp.
Cu2+
complete inhibition at 1 mM
Acinetobacter sp.
EDTA
-
Acinetobacter sp.
Hg2+
complete inhibition at 1 mM
Acinetobacter sp.
N-ethylmaleimide
-
Acinetobacter sp.
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.7
-
NADP+
at pH 8.0 and 37°C
Acinetobacter sp.
2.6
-
L-fucose
at pH 8.0 and 37°C
Acinetobacter sp.
2.7
-
NAD+
at pH 8.0 and 37°C
Acinetobacter sp.
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
23000
-
gel filtration
Acinetobacter sp.
25000
-
1 * 25000, SDS-PAGE
Acinetobacter sp.
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
L-fucose + NADP+
Acinetobacter sp.
100% activity
L-fuconolactone + NADPH + H+
-
-
?
L-fucose + NADP+
Acinetobacter sp. SA-134
100% activity
L-fuconolactone + NADPH + H+
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Acinetobacter sp.
-
-
-
Acinetobacter sp. SA-134
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
DEAE-Sepharose column chromatography, phenyl-Toyopearl column chromatography, Q-Sepharose column chromatography, Superdex 200HR gel filtration, and Mono Q column chromatography
Acinetobacter sp.
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.3
-
cell-free extract, at pH 8.0 and 37°C
Acinetobacter sp.
217
-
after 94.3fold purification, at pH 8.0 and 37°C
Acinetobacter sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
D-arabinose + NADP+
good substrate with 65% activity compared to L-fucose
741316
Acinetobacter sp.
D-arabinolactone + NADPH + H+
-
-
-
?
D-arabinose + NADP+
good substrate with 65% activity compared to L-fucose
741316
Acinetobacter sp. SA-134
D-arabinolactone + NADPH + H+
-
-
-
?
L-fucose + NAD+
-
741316
Acinetobacter sp.
L-fuconolactone + NADH + H+
-
-
-
?
L-fucose + NAD+
-
741316
Acinetobacter sp. SA-134
L-fuconolactone + NADH + H+
-
-
-
?
L-fucose + NADP+
good substrate
741316
Acinetobacter sp.
L-fuconolactone + NADPH + H+
-
-
-
?
L-fucose + NADP+
100% activity
741316
Acinetobacter sp.
L-fuconolactone + NADPH + H+
-
-
-
?
L-fucose + NADP+
good substrate
741316
Acinetobacter sp. SA-134
L-fuconolactone + NADPH + H+
-
-
-
?
L-fucose + NADP+
100% activity
741316
Acinetobacter sp. SA-134
L-fuconolactone + NADPH + H+
-
-
-
?
L-galactose + NADP+
L-galactose is a poor substrate with 11% activity compared to L-fucose
741316
Acinetobacter sp.
?
-
-
-
?
additional information
no activity with D-glucose, D-fructose, D-galactose, L-arabinose, D-mannose, L-rhamnose, D-xylose, maltose, sucrose, and lactose
741316
Acinetobacter sp.
?
-
-
-
?
additional information
no activity with D-glucose, D-fructose, D-galactose, L-arabinose, D-mannose, L-rhamnose, D-xylose, maltose, sucrose, and lactose
741316
Acinetobacter sp. SA-134
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 25000, SDS-PAGE
Acinetobacter sp.
Synonyms
Synonyms
Commentary
Organism
L-fucose dehydrogenase
-
Acinetobacter sp.
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
50
-
-
Acinetobacter sp.
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
35
-
the enzyme is stable below 35°C for 60 min
Acinetobacter sp.
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Acinetobacter sp.
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
6
10
the enzyme is stable between pH 6.0 and 10.0 for 60 min
Acinetobacter sp.
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
the enzyme acts on both NAD+ and NADP+ in similar manner
Acinetobacter sp.
NADP+
the enzyme acts on both NAD+ and NADP+ in similar manner
Acinetobacter sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
the enzyme acts on both NAD+ and NADP+ in similar manner
Acinetobacter sp.
NADP+
the enzyme acts on both NAD+ and NADP+ in similar manner
Acinetobacter sp.
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Ag+
complete inhibition at 1 mM
Acinetobacter sp.
Co2+
complete inhibition at 1 mM
Acinetobacter sp.
Cu2+
complete inhibition at 1 mM
Acinetobacter sp.
EDTA
-
Acinetobacter sp.
Hg2+
complete inhibition at 1 mM
Acinetobacter sp.
N-ethylmaleimide
-
Acinetobacter sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.7
-
NADP+
at pH 8.0 and 37°C
Acinetobacter sp.
2.6
-
L-fucose
at pH 8.0 and 37°C
Acinetobacter sp.
2.7
-
NAD+
at pH 8.0 and 37°C
Acinetobacter sp.
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
23000
-
gel filtration
Acinetobacter sp.
25000
-
1 * 25000, SDS-PAGE
Acinetobacter sp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
L-fucose + NADP+
Acinetobacter sp.
100% activity
L-fuconolactone + NADPH + H+
-
-
?
L-fucose + NADP+
Acinetobacter sp. SA-134
100% activity
L-fuconolactone + NADPH + H+
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
DEAE-Sepharose column chromatography, phenyl-Toyopearl column chromatography, Q-Sepharose column chromatography, Superdex 200HR gel filtration, and Mono Q column chromatography
Acinetobacter sp.
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.3
-
cell-free extract, at pH 8.0 and 37°C
Acinetobacter sp.
217
-
after 94.3fold purification, at pH 8.0 and 37°C
Acinetobacter sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
D-arabinose + NADP+
good substrate with 65% activity compared to L-fucose
741316
Acinetobacter sp.
D-arabinolactone + NADPH + H+
-
-
-
?
D-arabinose + NADP+
good substrate with 65% activity compared to L-fucose
741316
Acinetobacter sp. SA-134
D-arabinolactone + NADPH + H+
-
-
-
?
L-fucose + NAD+
-
741316
Acinetobacter sp.
L-fuconolactone + NADH + H+
-
-
-
?
L-fucose + NAD+
-
741316
Acinetobacter sp. SA-134
L-fuconolactone + NADH + H+
-
-
-
?
L-fucose + NADP+
good substrate
741316
Acinetobacter sp.
L-fuconolactone + NADPH + H+
-
-
-
?
L-fucose + NADP+
100% activity
741316
Acinetobacter sp.
L-fuconolactone + NADPH + H+
-
-
-
?
L-fucose + NADP+
good substrate
741316
Acinetobacter sp. SA-134
L-fuconolactone + NADPH + H+
-
-
-
?
L-fucose + NADP+
100% activity
741316
Acinetobacter sp. SA-134
L-fuconolactone + NADPH + H+
-
-
-
?
L-galactose + NADP+
L-galactose is a poor substrate with 11% activity compared to L-fucose
741316
Acinetobacter sp.
?
-
-
-
?
additional information
no activity with D-glucose, D-fructose, D-galactose, L-arabinose, D-mannose, L-rhamnose, D-xylose, maltose, sucrose, and lactose
741316
Acinetobacter sp.
?
-
-
-
?
additional information
no activity with D-glucose, D-fructose, D-galactose, L-arabinose, D-mannose, L-rhamnose, D-xylose, maltose, sucrose, and lactose
741316
Acinetobacter sp. SA-134
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 25000, SDS-PAGE
Acinetobacter sp.
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
50
-
-
Acinetobacter sp.
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
35
-
the enzyme is stable below 35°C for 60 min
Acinetobacter sp.
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Acinetobacter sp.
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
6
10
the enzyme is stable between pH 6.0 and 10.0 for 60 min
Acinetobacter sp.
Other publictions for EC 1.1.1.122
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741316
Ohshiro
Production and characterizatio ...
Acinetobacter sp., Acinetobacter sp. SA-134
Prep. Biochem. Biotechnol.
44
382-391
2014
-
-
-
-
-
-
6
3
-
-
2
2
-
8
-
-
1
-
-
-
2
-
11
1
1
1
-
1
-
1
-
1
2
-
-
-
-
-
-
2
-
-
-
-
6
-
3
-
-
2
2
-
-
-
1
-
-
2
-
11
1
1
-
1
-
1
-
1
-
-
-
-
-
-
-
681445
Brouns
Crystal structure and biochemi ...
Saccharolobus solfataricus, Saccharolobus solfataricus P2
J. Mol. Biol.
371
1249-1260
2007
-
-
-
1
-
-
-
-
-
1
-
-
-
3
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
719837
Brouns
Identification of the missing ...
Saccharolobus solfataricus, Saccharolobus solfataricus P2
J. Biol. Chem.
281
27378-27388
2006
-
-
1
-
-
-
-
-
-
1
2
-
-
3
-
-
-
-
-
-
-
-
2
1
1
1
-
2
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
2
1
1
-
2
-
1
-
-
-
-
-
-
-
-
-
655086
Bakke
-
Improvement in thermal stabili ...
Pseudomonas sp., Pseudomonas sp. No. 1143
Biotechnol. Lett.
24
1075-1078
2002
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
1
-
-
2
-
-
-
-
1
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
285798
Itoh
-
Purification and characterizat ...
Agrobacterium tumefaciens, Agrobacterium tumefaciens K-28
J. Ferment. Bioeng.
77
100-102
1994
-
-
-
-
-
-
4
5
-
-
1
6
-
2
-
-
1
-
-
1
2
-
10
-
-
1
-
1
-
1
-
1
1
-
-
-
-
-
-
1
-
-
-
-
4
-
5
-
-
1
6
-
-
-
1
-
1
2
-
10
-
1
-
1
-
1
-
1
-
-
-
-
-
-
-
285799
Yamamoto-Otake
Cloning and sequencing of the ...
Pseudomonas sp.
Biosci. Biotechnol. Biochem.
58
2281-2282
1994
-
-
1
-
-
-
-
-
-
-
-
1
-
9
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285800
Tsuji
Purification and some properti ...
Agrobacterium tumefaciens
Biochim. Biophys. Acta
1117
167-173
1992
-
1
-
-
-
1
4
4
-
-
3
3
-
10
-
-
1
-
-
1
1
1
4
1
-
-
-
1
-
-
1
1
2
-
-
-
-
1
-
2
-
-
1
-
4
-
4
-
-
3
3
-
-
-
1
-
1
1
1
4
1
-
-
1
-
-
1
1
-
-
-
-
-
-
-
285801
Guimaraes
-
Purification and general prope ...
Aureobasidium pullulans
Arq. Biol. Tecnol. (Curitiba)
32
575-587
1989
-
-
-
-
-
-
2
4
-
-
1
3
-
1
-
-
1
-
-
1
-
1
3
1
-
-
-
1
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
2
-
4
-
-
1
3
-
-
-
1
-
1
-
1
3
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
285802
Sasajima
Oxidation of L-glucose by a ps ...
Trinickia caryophylli
Biochim. Biophys. Acta
571
120-126
1979
-
-
-
-
-
1
4
4
-
-
1
7
-
1
-
-
1
-
-
1
1
1
7
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
1
-
4
-
4
-
-
1
7
-
-
-
1
-
1
1
1
7
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
285805
Endo
Isolation and characterization ...
Oryctolagus cuniculus
J. Biochem.
86
1559-1565
1979
-
-
-
-
-
-
4
3
-
-
1
4
-
6
-
-
1
-
-
3
1
1
4
-
-
1
-
1
-
1
-
1
1
-
-
-
-
-
-
1
-
-
-
-
4
-
3
-
-
1
4
-
-
-
1
-
3
1
1
4
-
1
-
1
-
1
-
1
-
-
-
-
-
-
-
285803
Mobley
The physical properties of NAD ...
Ovis aries aries
Arch. Biochem. Biophys.
186
184-188
1978
5
-
-
-
-
1
3
1
-
-
3
2
-
3
-
-
1
-
-
2
-
1
2
1
-
-
-
2
-
-
-
-
1
-
-
-
5
-
-
1
-
-
1
-
3
-
1
-
-
3
2
-
-
-
1
-
2
-
1
2
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
285804
Mobley
L-Fucose dehydrogenase from sh ...
Ovis aries aries
Methods Enzymol.
41B
173-177
1975
-
-
-
-
-
1
-
6
-
-
1
4
-
3
-
-
1
-
-
2
-
1
4
-
-
-
-
1
-
1
-
-
1
-
-
-
-
-
-
1
-
-
1
-
-
-
6
-
-
1
4
-
-
-
1
-
2
-
1
4
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
285806
Schacter
Isolation of diphosphopyridine ...
Sus scrofa
J. Biol. Chem.
244
4785-4792
1969
1
-
-
-
-
-
4
7
-
-
-
6
-
3
-
-
1
-
-
2
1
-
7
-
-
-
-
-
-
1
-
-
1
-
-
-
1
-
-
1
-
-
-
-
4
-
7
-
-
-
6
-
-
-
1
-
2
1
-
7
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-