Data extracted from this reference:
Inhibitors
Ag+
complete inhibition at 1 mM
Acinetobacter sp.
Co2+
complete inhibition at 1 mM
Acinetobacter sp.
Cu2+
complete inhibition at 1 mM
Acinetobacter sp.
Hg2+
complete inhibition at 1 mM
Acinetobacter sp.
N-ethylmaleimide
Acinetobacter sp.
KM Value [mM]
1.7
NADP+
at pH 8.0 and 37°C
Acinetobacter sp.
2.6
L-fucose
at pH 8.0 and 37°C
Acinetobacter sp.
2.7
NAD+
at pH 8.0 and 37°C
Acinetobacter sp.
Molecular Weight [Da]
23000
gel filtration
Acinetobacter sp.
25000
1 * 25000, SDS-PAGE
Acinetobacter sp.
Natural Substrates/ Products (Substrates)
L-fucose + NADP+
Acinetobacter sp.
100% activity
L-fuconolactone + NADPH + H+
?
L-fucose + NADP+
Acinetobacter sp. SA-134
100% activity
L-fuconolactone + NADPH + H+
?
Organism
Purification (Commentary)
DEAE-Sepharose column chromatography, phenyl-Toyopearl column chromatography, Q-Sepharose column chromatography, Superdex 200HR gel filtration, and Mono Q column chromatography
Acinetobacter sp.
Specific Activity [micromol/min/mg]
2.3
cell-free extract, at pH 8.0 and 37°C
Acinetobacter sp.
217
after 94.3fold purification, at pH 8.0 and 37°C
Acinetobacter sp.
Substrates and Products (Substrate)
D-arabinose + NADP+
good substrate with 65% activity compared to L-fucose
741316
Acinetobacter sp.
D-arabinolactone + NADPH + H+
?
D-arabinose + NADP+
good substrate with 65% activity compared to L-fucose
741316
Acinetobacter sp. SA-134
D-arabinolactone + NADPH + H+
?
L-fucose + NAD+
741316
Acinetobacter sp.
L-fuconolactone + NADH + H+
?
L-fucose + NAD+
741316
Acinetobacter sp. SA-134
L-fuconolactone + NADH + H+
?
L-fucose + NADP+
good substrate
741316
Acinetobacter sp.
L-fuconolactone + NADPH + H+
?
L-fucose + NADP+
100% activity
741316
Acinetobacter sp.
L-fuconolactone + NADPH + H+
?
L-fucose + NADP+
good substrate
741316
Acinetobacter sp. SA-134
L-fuconolactone + NADPH + H+
?
L-fucose + NADP+
100% activity
741316
Acinetobacter sp. SA-134
L-fuconolactone + NADPH + H+
?
L-galactose + NADP+
L-galactose is a poor substrate with 11% activity compared to L-fucose
741316
Acinetobacter sp.
?
?
additional information
no activity with D-glucose, D-fructose, D-galactose, L-arabinose, D-mannose, L-rhamnose, D-xylose, maltose, sucrose, and lactose
741316
Acinetobacter sp.
?
?
additional information
no activity with D-glucose, D-fructose, D-galactose, L-arabinose, D-mannose, L-rhamnose, D-xylose, maltose, sucrose, and lactose
741316
Acinetobacter sp. SA-134
?
?
Subunits
monomer
1 * 25000, SDS-PAGE
Acinetobacter sp.
Synonyms
L-fucose dehydrogenase
Acinetobacter sp.
Temperature Optimum [°C]
Temperature Stability [°C]
35
the enzyme is stable below 35°C for 60 min
Acinetobacter sp.
pH Optimum
pH Stability
6
10
the enzyme is stable between pH 6.0 and 10.0 for 60 min
Acinetobacter sp.
Cofactor
NAD+
the enzyme acts on both NAD+ and NADP+ in similar manner
Acinetobacter sp.
NADP+
the enzyme acts on both NAD+ and NADP+ in similar manner
Acinetobacter sp.
Cofactor (protein specific)
NAD+
the enzyme acts on both NAD+ and NADP+ in similar manner
Acinetobacter sp.
NADP+
the enzyme acts on both NAD+ and NADP+ in similar manner
Acinetobacter sp.
Inhibitors (protein specific)
Ag+
complete inhibition at 1 mM
Acinetobacter sp.
Co2+
complete inhibition at 1 mM
Acinetobacter sp.
Cu2+
complete inhibition at 1 mM
Acinetobacter sp.
Hg2+
complete inhibition at 1 mM
Acinetobacter sp.
N-ethylmaleimide
Acinetobacter sp.
KM Value [mM] (protein specific)
1.7
NADP+
at pH 8.0 and 37°C
Acinetobacter sp.
2.6
L-fucose
at pH 8.0 and 37°C
Acinetobacter sp.
2.7
NAD+
at pH 8.0 and 37°C
Acinetobacter sp.
Molecular Weight [Da] (protein specific)
23000
gel filtration
Acinetobacter sp.
25000
1 * 25000, SDS-PAGE
Acinetobacter sp.
Natural Substrates/ Products (Substrates) (protein specific)
L-fucose + NADP+
Acinetobacter sp.
100% activity
L-fuconolactone + NADPH + H+
?
L-fucose + NADP+
Acinetobacter sp. SA-134
100% activity
L-fuconolactone + NADPH + H+
?
Purification (Commentary) (protein specific)
DEAE-Sepharose column chromatography, phenyl-Toyopearl column chromatography, Q-Sepharose column chromatography, Superdex 200HR gel filtration, and Mono Q column chromatography
Acinetobacter sp.
Specific Activity [micromol/min/mg] (protein specific)
2.3
cell-free extract, at pH 8.0 and 37°C
Acinetobacter sp.
217
after 94.3fold purification, at pH 8.0 and 37°C
Acinetobacter sp.
Substrates and Products (Substrate) (protein specific)
D-arabinose + NADP+
good substrate with 65% activity compared to L-fucose
741316
Acinetobacter sp.
D-arabinolactone + NADPH + H+
?
D-arabinose + NADP+
good substrate with 65% activity compared to L-fucose
741316
Acinetobacter sp. SA-134
D-arabinolactone + NADPH + H+
?
L-fucose + NAD+
741316
Acinetobacter sp.
L-fuconolactone + NADH + H+
?
L-fucose + NAD+
741316
Acinetobacter sp. SA-134
L-fuconolactone + NADH + H+
?
L-fucose + NADP+
good substrate
741316
Acinetobacter sp.
L-fuconolactone + NADPH + H+
?
L-fucose + NADP+
100% activity
741316
Acinetobacter sp.
L-fuconolactone + NADPH + H+
?
L-fucose + NADP+
good substrate
741316
Acinetobacter sp. SA-134
L-fuconolactone + NADPH + H+
?
L-fucose + NADP+
100% activity
741316
Acinetobacter sp. SA-134
L-fuconolactone + NADPH + H+
?
L-galactose + NADP+
L-galactose is a poor substrate with 11% activity compared to L-fucose
741316
Acinetobacter sp.
?
?
additional information
no activity with D-glucose, D-fructose, D-galactose, L-arabinose, D-mannose, L-rhamnose, D-xylose, maltose, sucrose, and lactose
741316
Acinetobacter sp.
?
?
additional information
no activity with D-glucose, D-fructose, D-galactose, L-arabinose, D-mannose, L-rhamnose, D-xylose, maltose, sucrose, and lactose
741316
Acinetobacter sp. SA-134
?
?
Subunits (protein specific)
monomer
1 * 25000, SDS-PAGE
Acinetobacter sp.
Temperature Optimum [°C] (protein specific)
Temperature Stability [°C] (protein specific)
35
the enzyme is stable below 35°C for 60 min
Acinetobacter sp.
pH Optimum (protein specific)
pH Stability (protein specific)
6
10
the enzyme is stable between pH 6.0 and 10.0 for 60 min
Acinetobacter sp.
Other publictions for EC 1.1.1.122
741316
Ohshiro
Production and characterizatio ...
Acinetobacter sp., Acinetobacter sp. SA-134
Prep. Biochem. Biotechnol.
44
382-391
2014
-
-
-
-
-
-
6
3
-
-
2
2
-
8
-
-
1
-
-
-
2
-
11
1
1
1
-
1
-
1
-
1
2
-
-
-
-
-
-
2
-
-
-
-
6
-
3
-
-
2
2
-
-
-
1
-
-
2
-
11
1
1
-
1
-
1
-
1
-
-
-
-
-
-
-
681445
Brouns
Crystal structure and biochemi ...
Saccharolobus solfataricus, Saccharolobus solfataricus P2
J. Mol. Biol.
371
1249-1260
2007
-
-
-
1
-
-
-
-
-
1
-
-
-
3
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
719837
Brouns
Identification of the missing ...
Saccharolobus solfataricus, Saccharolobus solfataricus P2
J. Biol. Chem.
281
27378-27388
2006
-
-
1
-
-
-
-
-
-
1
2
-
-
3
-
-
-
-
-
-
-
-
2
1
1
1
-
2
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
2
1
1
-
2
-
1
-
-
-
-
-
-
-
-
-
655086
Bakke
-
Improvement in thermal stabili ...
Pseudomonas sp., Pseudomonas sp. No. 1143
Biotechnol. Lett.
24
1075-1078
2002
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
1
-
-
2
-
-
-
-
1
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
285798
Itoh
-
Purification and characterizat ...
Agrobacterium tumefaciens, Agrobacterium tumefaciens K-28
J. Ferment. Bioeng.
77
100-102
1994
-
-
-
-
-
-
4
5
-
-
1
6
-
2
-
-
1
-
-
1
2
-
10
-
-
1
-
1
-
1
-
1
1
-
-
-
-
-
-
1
-
-
-
-
4
-
5
-
-
1
6
-
-
-
1
-
1
2
-
10
-
1
-
1
-
1
-
1
-
-
-
-
-
-
-
285799
Yamamoto-Otake
Cloning and sequencing of the ...
Pseudomonas sp.
Biosci. Biotechnol. Biochem.
58
2281-2282
1994
-
-
1
-
-
-
-
-
-
-
-
1
-
9
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285800
Tsuji
Purification and some properti ...
Agrobacterium tumefaciens
Biochim. Biophys. Acta
1117
167-173
1992
-
1
-
-
-
1
4
4
-
-
3
3
-
10
-
-
1
-
-
1
1
1
4
1
-
-
-
1
-
-
1
1
2
-
-
-
-
1
-
2
-
-
1
-
4
-
4
-
-
3
3
-
-
-
1
-
1
1
1
4
1
-
-
1
-
-
1
1
-
-
-
-
-
-
-
285801
Guimaraes
-
Purification and general prope ...
Aureobasidium pullulans
Arq. Biol. Tecnol. (Curitiba)
32
575-587
1989
-
-
-
-
-
-
2
4
-
-
1
3
-
1
-
-
1
-
-
1
-
1
3
1
-
-
-
1
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
2
-
4
-
-
1
3
-
-
-
1
-
1
-
1
3
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
285802
Sasajima
Oxidation of L-glucose by a ps ...
Trinickia caryophylli
Biochim. Biophys. Acta
571
120-126
1979
-
-
-
-
-
1
4
4
-
-
1
7
-
1
-
-
1
-
-
1
1
1
7
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
1
-
4
-
4
-
-
1
7
-
-
-
1
-
1
1
1
7
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
285805
Endo
Isolation and characterization ...
Oryctolagus cuniculus
J. Biochem.
86
1559-1565
1979
-
-
-
-
-
-
4
3
-
-
1
4
-
6
-
-
1
-
-
3
1
1
4
-
-
1
-
1
-
1
-
1
1
-
-
-
-
-
-
1
-
-
-
-
4
-
3
-
-
1
4
-
-
-
1
-
3
1
1
4
-
1
-
1
-
1
-
1
-
-
-
-
-
-
-
285803
Mobley
The physical properties of NAD ...
Ovis aries aries
Arch. Biochem. Biophys.
186
184-188
1978
5
-
-
-
-
1
3
1
-
-
3
2
-
3
-
-
1
-
-
2
-
1
2
1
-
-
-
2
-
-
-
-
1
-
-
-
5
-
-
1
-
-
1
-
3
-
1
-
-
3
2
-
-
-
1
-
2
-
1
2
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
285804
Mobley
L-Fucose dehydrogenase from sh ...
Ovis aries aries
Methods Enzymol.
41B
173-177
1975
-
-
-
-
-
1
-
6
-
-
1
4
-
3
-
-
1
-
-
2
-
1
4
-
-
-
-
1
-
1
-
-
1
-
-
-
-
-
-
1
-
-
1
-
-
-
6
-
-
1
4
-
-
-
1
-
2
-
1
4
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
285806
Schacter
Isolation of diphosphopyridine ...
Sus scrofa
J. Biol. Chem.
244
4785-4792
1969
1
-
-
-
-
-
4
7
-
-
-
6
-
3
-
-
1
-
-
2
1
-
7
-
-
-
-
-
-
1
-
-
1
-
-
-
1
-
-
1
-
-
-
-
4
-
7
-
-
-
6
-
-
-
1
-
2
1
-
7
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-