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Literature summary for 1.1.1.123 extracted from

  • Schiessl, J.; Kosciow, K.; Garschagen, L.S.; Hoffmann, J.J.; Heymuth, J.; Franke, T.; Deppenmeier, U.
    Degradation of the low-calorie sugar substitute 5-ketofructose by different bacteria (2021), Appl. Microbiol. Biotechnol., 105, 2441-2453 .
    View publication on PubMed
Search for more literature for 1.1.1.123

Cloned(Commentary)

Cloned (Comment) Organism
heterologously overproduced in Escherichia coli Clostridium pasteurianum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3
-
 5-dehydro-D-fructose pH and temperature not specified in the publication Clostridium pasteurianum
12.4
-
 NADPH pH and temperature not specified in the publication Clostridium pasteurianum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35900
-
 molecular mass including Strep-tag Clostridium pasteurianum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-sorbose + NADP+ Clostridium pasteurianum
-
 5-dehydro-D-fructose + NADPH + H+
-
 ?
L-sorbose + NADP+ Clostridium pasteurianum DSM 525
-
 5-dehydro-D-fructose + NADPH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Clostridium pasteurianum AJA48273.1
-
-
Clostridium pasteurianum DSM 525 AJA48273.1
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Clostridium pasteurianum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
48.9
-
 pH and temperature not specified in the publication Clostridium pasteurianum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-phenyl-1,2-propandione + NADP+ 14.8% of the specific activity as compared to 5-dehydro-D-fructose Clostridium pasteurianum ?
-
 ?
1-phenyl-1,2-propandione + NADP+ 14.8% of the specific activity as compared to 5-dehydro-D-fructose Clostridium pasteurianum DSM 525 ?
-
 ?
5-dehydro-D-fructose + NADPH + H+
-
 Clostridium pasteurianum L-sorbose + NADP+
-
 ?
5-dehydro-D-fructose + NADPH + H+
-
 Clostridium pasteurianum DSM 525 L-sorbose + NADP+
-
 ?
diacetyl + NADP+ 38% of the specific activity as compared to 5-dehydro-D-fructose Clostridium pasteurianum ?
-
 ?
diacetyl + NADP+ 38% of the specific activity as compared to 5-dehydro-D-fructose Clostridium pasteurianum DSM 525 ?
-
 ?
L-sorbose + NADP+
-
 Clostridium pasteurianum 5-dehydro-D-fructose + NADPH + H+
-
 ?
L-sorbose + NADP+
-
 Clostridium pasteurianum DSM 525 5-dehydro-D-fructose + NADPH + H+
-
 ?
pyruvaldehyde + NADP+ 5% of the specific activity as compared to 5-dehydro-D-fructose Clostridium pasteurianum ?
-
 ?
pyruvaldehyde + NADP+ 5% of the specific activity as compared to 5-dehydro-D-fructose Clostridium pasteurianum DSM 525 ?
-
 ?

Subunits

Subunits Comment Organism
homotetramer 4 * 35000, SDS-PAGE Clostridium pasteurianum

Synonyms

Synonyms Comment Organism
AJA48273.1
-
 Clostridium pasteurianum
CP-AKR
-
 Clostridium pasteurianum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Clostridium pasteurianum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
29.3
-
 5-dehydro-D-fructose pH and temperature not specified in the publication Clostridium pasteurianum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Clostridium pasteurianum

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Clostridium pasteurianum

General Information

General Information Comment Organism
metabolism an important function in the degradation of this substrate and might be responsible for the formation of fructose, which is then channelled into the central metabolism of Clostridium pasteurianum Clostridium pasteurianum