Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
enzyme overproduced using 3 different plasmid vectors containing algD under control of the tac promoter expressed in non-mucoid strain PAO1 and mucoid strain PAO568, pPT3 and pPT4 are forward and reverse clones contain leader sequence with possible transcriptional/translational regulatory sequences of algD gene, no significant activity with pMMB66EH and pPT4 in either IPTG induced or non induced Escherichia coli, mucoid and non-mucoid Pseudomonas aeruginosa strains | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00015 | - |
+/-0.00001, S17-1/pPT4, with IPTG | Pseudomonas aeruginosa |
0.00017 | - |
+/-0.00002, S17-1/pMMB66EH and S17-1/pPT4 without IPTG | Pseudomonas aeruginosa |
0.00022 | - |
+/-0.00001, S17-1/pMMB66EH, with IPTG | Pseudomonas aeruginosa |
0.00034 | - |
+/-0.00006, S17-1/pPT3, without IPTG | Pseudomonas aeruginosa |
0.0009 | - |
+/-0.0003, PAO1/pMMB66EH, without IPTG | Pseudomonas aeruginosa |
0.0009 | - |
+/-0.0002, PAO1/pMMB66EH, with IPTG | Pseudomonas aeruginosa |
0.013 | - |
+/-0.003, PAO1/pPT3, without IPTG | Pseudomonas aeruginosa |
0.014 | - |
+/-0.0014, PAO1/pPT4, with IPTG | Pseudomonas aeruginosa |
0.0147 | - |
+/-0.0014, PAO1/pPT4, without IPTG | Pseudomonas aeruginosa |
0.0176 | - |
+/-0.0009, PAO568/pMMB66EH, with IPTG | Pseudomonas aeruginosa |
0.0182 | - |
+/-0.0003, PAO568/pMMB66EH, without IPTG | Pseudomonas aeruginosa |
0.0223 | - |
+/-0.0011, PAO568/pPT3, without IPTG | Pseudomonas aeruginosa |
0.0251 | - |
+/-0.0027, PAO568/pPT4, without IPTG | Pseudomonas aeruginosa |
0.0254 | - |
+/-0.004, PAO568/pPT4, with IPTG | Pseudomonas aeruginosa |
0.1895 | - |
+/-0.00702, S17-1/pPT3, with IPTG | Pseudomonas aeruginosa |
0.2041 | - |
+/-0.00932, PAO1/pPT3, with IPTG | Pseudomonas aeruginosa |
0.2378 | - |
+/-0.0552, PAO568/pPT3, with IPTG | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-mannose + NAD+ + H2O | - |
Pseudomonas aeruginosa | GDP-mannuronate + NADH | - |
ir |
Subunits | Comment | Organism |
---|---|---|
hexamer | - |
Pseudomonas aeruginosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Pseudomonas aeruginosa |