Literature summary for 1.1.1.140 extracted from

Liss, M.; Horwitz, S.B.; Kaplan, N.O.
D-Mannitol 1-phosphate dehydrogenase and D-sorbitol 6-phosphate dehydrogenase in Aerobacter aerogenes (1962), J. Biol. Chem., 237, 1342-1350.

Search for more literature for 1.1.1.140

Inhibitors

Inhibitors	Comment	Organism	Structure
CO32-	-	Klebsiella aerogenes

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.18	-	NADH	-	Klebsiella aerogenes
0.42	-	NAD+	-	Klebsiella aerogenes
6.25	-	sorbitol 6-phosphate	-	Klebsiella aerogenes
10.5	-	D-fructose 6-phosphate	-	Klebsiella aerogenes

Organism

Organism	UniProt	Comment	Textmining
Klebsiella aerogenes	-	enzyme induced by growth on D-sorbitol	-

Purification (Commentary)

Purification (Comment)	Organism
approximately 100 fold purification	Klebsiella aerogenes

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	-	Klebsiella aerogenes

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-dulcitol 6-phosphate + NAD+	at 3% of reaction reaction with sorbitol 6-phosphate	Klebsiella aerogenes	?	-	?
D-sorbitol 6-phosphate + NAD+	high specificity for D-sorbitol 6-phosphate	Klebsiella aerogenes	D-fructose 6-phosphate + NADH + H+	-	r

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
9	-	Tris-HCl buffer	Klebsiella aerogenes

pH Range

pH Minimum	pH Maximum	Comment	Organism
additional information	-	in Tris buffer 32times greater activity than in NaHCO3 buffer	Klebsiella aerogenes

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	inhibited by alteration of the 6-amino position of the adenine moiety of NAD+	Klebsiella aerogenes

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Release 2023.1 (January 2023)