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Literature summary for 1.1.1.144 extracted from

  • Ballal, N.R.; Bhattacharyya, P.K.; Rangachari, P.N.
    Microbiological transformations of terpenes. XIV. Purification & properties of perillyl alcohol dehydrogenase (1968), Indian J. Biochem., 5, 1-6.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information dehydrogenase is induced by limonene, alpha-pinene, delta-p-menthene and to lesser extend by p-cymene Pseudomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 0.005 mM, complete inhibition, prevented by 10 mM glutathione Pseudomonas sp.
Cu2+ 5 mM, complete inhibition, prevented by 5 mM glutathione Pseudomonas sp.
Hg2+ 0.005 mM, complete inhibition, prevented by 10 mM glutathione Pseudomonas sp.
iodoacetate 10 mM, 95% inhibition, prevented by 10 mM glutathione Pseudomonas sp.
additional information glucose represses the induction of the enzyme by limonene Pseudomonas sp.
NADH competitive inhibition Pseudomonas sp.
Ni2+ 2 mM, 75% inhibition, complete reversion with 5 mM EDTA Pseudomonas sp.
o-phenanthroline at concentrations higher than 3 mM and low concentration of NAD+, about 50% inhibition Pseudomonas sp.
p-hydroxymercuribenzoate 0.005 mM, complete inhibition, prevented by 10 mM glutathione Pseudomonas sp.
Zn2+ 2 mM, 75% inhibition, complete reversion with 10 mM EDTA Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Pseudomonas sp.
0.077
-
NAD+ at pH 8.6 Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
perillyl alcohol + NAD+ Pseudomonas sp. catalyzes dehydrogenation reaction which is a part of the catabolic sequence of alpha-pinenes and beta-pinenes, limonene, DELTA1-p-methene and p-cymene perillyl aldehyde + NADH
-
?
perillyl alcohol + NAD+ Pseudomonas sp. PL- catalyzes dehydrogenation reaction which is a part of the catabolic sequence of alpha-pinenes and beta-pinenes, limonene, DELTA1-p-methene and p-cymene perillyl aldehyde + NADH
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
PL-strain
-
Pseudomonas sp. PL-
-
PL-strain
-

Purification (Commentary)

Purification (Comment) Organism
PL-strain Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [Āµmol/min/mg] Specific Activity Maximum [Āµmol/min/mg] Comment Organism
13
-
DEAE-eluate Pseudomonas sp.

Storage Stability

Storage Stability Organism
-20Ā°C, for at least 3 days, DEAE eluate Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no substrates are p-menthan-7-ol, DELTA1-p-menthen-10-ol, DELTA8-menthen-10-ol, p-menthan-10-ol, myrtenol, isoborneol, borneol, cyclohexenol, o-menthyl benzyl alcohol, phthalyl alcohol, salicylic alcohol, 7-methyl cumic alcohol, ethanol, methanol Pseudomonas sp. ?
-
?
additional information no substrates are p-menthan-7-ol, DELTA1-p-menthen-10-ol, DELTA8-menthen-10-ol, p-menthan-10-ol, myrtenol, isoborneol, borneol, cyclohexenol, o-menthyl benzyl alcohol, phthalyl alcohol, salicylic alcohol, 7-methyl cumic alcohol, ethanol, methanol Pseudomonas sp. PL- ?
-
?
perillyl alcohol + NAD+ e.g.: 8-hydroxyphellandrol, cumic alcohol Pseudomonas sp. perillyl aldehyde + NADH
-
r
perillyl alcohol + NAD+ p-ethyl benzyl alcohol, p-methyl benzyl alcohol Pseudomonas sp. perillyl aldehyde + NADH
-
r
perillyl alcohol + NAD+ rate of the reverse reaction is about 20% of the rate of the forward reaction, oxidizes a number of primary alcohols with the alcohol group allylic to an endocyclic double bond and a 6-membered ring, either aromatic or hydroaromatic Pseudomonas sp. perillyl aldehyde + NADH
-
r
perillyl alcohol + NAD+ catalyzes dehydrogenation reaction which is a part of the catabolic sequence of alpha-pinenes and beta-pinenes, limonene, DELTA1-p-methene and p-cymene Pseudomonas sp. perillyl aldehyde + NADH
-
?
perillyl alcohol + NAD+ e.g.: 8-hydroxyphellandrol, cumic alcohol Pseudomonas sp. PL- perillyl aldehyde + NADH
-
r
perillyl alcohol + NAD+ p-ethyl benzyl alcohol, p-methyl benzyl alcohol Pseudomonas sp. PL- perillyl aldehyde + NADH
-
r
perillyl alcohol + NAD+ rate of the reverse reaction is about 20% of the rate of the forward reaction, oxidizes a number of primary alcohols with the alcohol group allylic to an endocyclic double bond and a 6-membered ring, either aromatic or hydroaromatic Pseudomonas sp. PL- perillyl aldehyde + NADH
-
r
perillyl alcohol + NAD+ catalyzes dehydrogenation reaction which is a part of the catabolic sequence of alpha-pinenes and beta-pinenes, limonene, DELTA1-p-methene and p-cymene Pseudomonas sp. PL- perillyl aldehyde + NADH
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
NADH + perillyl aldehyde Pseudomonas sp.
9.4
-
NAD+ + perillyl alcohol Pseudomonas sp.

pH Range

pH Minimum pH Maximum Comment Organism
5 8 pH 5: about 35% of activity maximum, pH 8: about 70% of activity maximum, NADH + perillyl aldehyde Pseudomonas sp.
6.3 10.5 at pH 6.3 and 10.5: about 10% of activity maximum, perillyl alcohol + NAD+ Pseudomonas sp.

Cofactor

Cofactor Comment Organism Structure
additional information inactive with NADP+ and with thionicotinamide adenine dinucleotide Pseudomonas sp.
NAD+ also active with some NAD+ analogues, e.g.: 3-acetylpyridine adenine dinucleotide, 3-pyridinealdehyde adenine dinucleotide, deamino-NAD Pseudomonas sp.
NADH
-
Pseudomonas sp.