Cloned (Comment) | Organism |
---|---|
expression in Sf9 cells | Homo sapiens |
expression of soluble wild-type and mutant isozyme 3beta-HSD1 in Spodoptera frugiperda Sf9 cells using the baculovirus transfection system | Homo sapiens |
expression of soluble wild-type and mutant isozyme 3beta-HSD2 in Spodoptera frugiperda Sf9 cells using the baculovirus transfection system | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
H154F | no activity | Homo sapiens |
H156Y | 194% of wild-type activity | Homo sapiens |
H156Y | site-directed mutagenesis of isozyme 1, construction of a chimeric Y154-P-H156-S-K158 motif of isozymes 1 and 2, the mutant shows altered kinetics compared to the wild-type isozyme 1, the kinetics are similar to those of isozyme 2 | Homo sapiens |
K158Q | no activity | Homo sapiens |
K158Q | site-directed mutagenesis of isozyme 1, inactive mutant showing no 3beta-hydroxysteroid dehydrogenase activity, but reduced isomerase activity | Homo sapiens |
K273Q | no activity | Homo sapiens |
K273Q | site-directed mutagenesis of isozyme 1, inactive mutant showing no 3beta-hydroxysteroid dehydrogenase activity, but reduced isomerase activity | Homo sapiens |
Y154F | site-directed mutagenesis of isozyme 1, inactive mutant showing no 3beta-hydroxysteroid dehydrogenase activity, but reduced isomerase activity | Homo sapiens |
Y269S | no activity | Homo sapiens |
Y269S | site-directed mutagenesis of isozyme 1, inactive mutant showing no 3beta-hydroxysteroid dehydrogenase activity, but reduced isomerase activity | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
epostane | inhibits the 3beta-HSD activity of the wild-type 1 isozyme with 1417fold greater affinity compared to the wild-type 2 isozyme and mutant H156Y; inhibits the 3beta-HSD activity of the wild-type 1 isozyme with 1417fold greater affinity compared to the wild-type 2 isozyme and mutant H156Y | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics recombinant wild-type isozyme and mutant H165Y, kinetics for the DELTA-5-3-ketosteroid isomerase, EC 5.3.3.1, activity | Homo sapiens | |
additional information | - |
additional information | kinetics recombinant wild-type isozymes and mutant H165Y, kinetics for the DELTA-5-3-ketosteroid isomerase, EC 5.3.3.1, activity | Homo sapiens | |
0.0028 | - |
pregnenolone | 27°C, pH 7.4 | Homo sapiens | |
0.0035 | - |
17alpha-hydroxypregnenolone | 27°C, pH 7.4 | Homo sapiens | |
0.0037 | - |
dehydroepiandrosterone | 27°C, pH 7.4 | Homo sapiens | |
0.0037 | - |
dehydroepiandrosterone | pH 7.4, 27°C, recombinant wild-type isozyme 3beta-HSD1 | Homo sapiens | |
0.018 | - |
17alpha-hydroxypregnenolone | 27°C, pH 7.4 | Homo sapiens | |
0.025 | - |
NAD+ | 27°C, pH 7.4, H156Y mutant 3beta-HSD1 | Homo sapiens | |
0.034 | - |
NAD+ | 27°C, pH 7.4 | Homo sapiens | |
0.042 | - |
dehydroepiandrosterone | 27°C, pH 7.4, H156Y mutant 3beta-HSD1 | Homo sapiens | |
0.0424 | - |
dehydroepiandrosterone | pH 7.4, 27°C, recombinant mutant H156Y | Homo sapiens | |
0.047 | - |
dehydroepiandrosterone | 27°C, pH 7.4 | Homo sapiens | |
0.0473 | - |
dehydroepiandrosterone | pH 7.4, 27°C, recombinant wild-type isozyme 3beta-HSD2 | Homo sapiens | |
0.05 | - |
pregnenolone | 27°C, pH 7.4 | Homo sapiens | |
0.086 | - |
NAD+ | 27°C, pH 7.4 | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
17alpha-hydroxypregnenolone + NAD+ | Homo sapiens | - |
17alpha-hydroxy-pregn-5-en-3,20-dione + NADH | - |
? | |
dehydroepiandrosterone + NAD+ | Homo sapiens | - |
androst-5-en-3,17-dione + NADH + H+ | - |
? | |
dehydroepiandrosterone + NAD+ | Homo sapiens | isozyme type 1 | ? | - |
? | |
additional information | Homo sapiens | the enzyme is essential for biosynthesis of all classes of steroid hormones, pathway overview | ? | - |
? | |
pregnenolone + NAD+ | Homo sapiens | - |
pregn-5-en-3,20-dione + NADH + H+ | - |
? | |
pregnenolone + NAD+ | Homo sapiens | isozyme type 2 | progesterone + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Homo sapiens | P14060 | 3beta-HSD1 | - |
Homo sapiens | P26439 | 3beta-HSD2 | - |
Purification (Comment) | Organism |
---|---|
recombinant 3beta-HSD1 | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a 3beta-hydroxy-DELTA5-steroid + NAD+ = a 3-oxo-DELTA5-steroid + NADH + H+ | catalytic residues are Lys159 and Tyr154, structure/function analysis of isozyme 1 | Homo sapiens | |
a 3beta-hydroxy-DELTA5-steroid + NAD+ = a 3-oxo-DELTA5-steroid + NADH + H+ | catalytic residues are Lys159 and Tyr154, structure/function analysis of isozyme 2 | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adrenal cortex | isozyme type 2 | Homo sapiens | - |
placenta | isozyme type 1 | Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
17alpha-hydroxypregnenolone + NAD+ | - |
Homo sapiens | 17alpha-hydroxy-pregn-5-en-3,20-dione + NADH | - |
? | |
dehydroepiandrosterone + NAD+ | - |
Homo sapiens | androst-5-en-3,17-dione + NADH + H+ | - |
? | |
dehydroepiandrosterone + NAD+ | - |
Homo sapiens | ? | - |
? | |
dehydroepiandrosterone + NAD+ | isozyme type 1 | Homo sapiens | ? | - |
? | |
additional information | the enzyme is essential for biosynthesis of all classes of steroid hormones, pathway overview | Homo sapiens | ? | - |
? | |
additional information | bifunctional enzyme that also catalyzes the reaction of the DELTA-5-3-ketosteroid isomerase, EC 5.3.3.1 | Homo sapiens | ? | - |
? | |
pregnenolone + NAD+ | - |
Homo sapiens | pregn-5-en-3,20-dione + NADH + H+ | - |
? | |
pregnenolone + NAD+ | isozyme type 2 | Homo sapiens | progesterone + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3beta-HSD1 | - |
Homo sapiens |
3beta-HSD22 | - |
Homo sapiens |
3beta-hydroxysteroid dehydrogenase type 2 | - |
Homo sapiens |
3beta-hydroxysteroid dehydrogenase type1 | - |
Homo sapiens |
type 1 3beta-hydroxysteroid dehydrogenase | - |
Homo sapiens |
type 2 3beta-hydroxysteroid dehydrogenase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
27 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
dehydroepiandrosterone | 27°C, pH 7.4 | Homo sapiens | |
0.062 | - |
dehydroepiandrosterone | pH 7.4, 27°C, recombinant wild-type isozyme 3beta-HSD1 | Homo sapiens | |
0.07 | - |
NAD+ | 27°C, pH 7.4 | Homo sapiens | |
0.08 | - |
NAD+ | 27°C, pH 7.4, H156Y mutant 3beta-HSD1 | Homo sapiens | |
0.115 | - |
dehydroepiandrosterone | pH 7.4, 27°C, recombinant wild-type isozyme 3beta-HSD2 | Homo sapiens | |
0.12 | - |
NAD+ | 27°C, pH 7.4 | Homo sapiens | |
0.12 | - |
dehydroepiandrosterone | 27°C, pH 7.4 | Homo sapiens | |
0.12 | - |
dehydroepiandrosterone | 27°C, pH 7.4, H156Y mutant 3beta-HSD1 | Homo sapiens | |
0.12 | - |
dehydroepiandrosterone | pH 7.4, 27°C, recombinant mutant H156Y | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |
9.7 | - |
- |
Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.4 | 9.7 | structure motif-dependent pH dependence, overview | Homo sapiens |
7.4 | - |
52% of maximal activity | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00007 | - |
epostane | 27°C, pH 7.4 | Homo sapiens | |
0.000071 | - |
epostane | pH 7.4, 27°C, recombinant wild-type isozyme 3beta-HSD1 | Homo sapiens | |
0.00098 | - |
epostane | 27°C, pH 7.4 | Homo sapiens | |
0.00098 | - |
epostane | pH 7.4, 27°C, recombinant wild-type isozyme 3beta-HSD2 | Homo sapiens | |
0.0012 | - |
epostane | 27°C, pH 7.4, H156Y mutant | Homo sapiens |