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Literature summary for 1.1.1.145 extracted from
- 3 beta-hydroxysteroid dehydrogenase isomerase (3beta-HSD) activity in the rat sciatic nerve: kinetic analysis and regulation by steroids (2003), J. Steroid Biochem. Mol. Biol., 85, 89-94.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression analysis of the enzyme in sciatic nerves | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| estradiol | 0.0005 mM, 40% inhibition, 0.001 mM, 62% inhibition | Rattus norvegicus |  |
| progesterone | 0.025 mM, 44% inhibition, 0.05 mM, 67% inhibition | Rattus norvegicus | |
| trilostane | 0.005 mM, 56% inhibition, 0.025 mM, 90% inhibition; potent inhibition | Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetic analysis | Rattus norvegicus | |
| 0.001 | - |
pregnenolone | 37°C | Rattus norvegicus | |
| 0.0011 | - |
pregnenolone | 37°C | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
x * 45000, SDS-PAGE | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Rattus norvegicus | enzyme regulation by steroids, overview | ? | - |
? | |
| pregnenolone + NAD+ | Rattus norvegicus | - |
pregn-5-en-3,20-dione + NADH + H+ | - |
? | |
| pregnenolone + NAD+ | Rattus norvegicus | - |
progesterone + NADH + H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
| Rattus norvegicus | - |
male SpragueDawley rats, type I and IV isozymes | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| sciatic nerve | - |
Rattus norvegicus | - |
| sciatic nerve | expression analysis | Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | enzyme regulation by steroids, overview | Rattus norvegicus | ? | - |
? | |
| pregnenolone + NAD+ | - |
Rattus norvegicus | pregn-5-en-3,20-dione + NADH + H+ | - |
? | |
| pregnenolone + NAD+ | - |
Rattus norvegicus | progesterone + NADH + H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 45000, SDS-PAGE | Rattus norvegicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 3beta-HSD | - |
Rattus norvegicus |
| 3beta-hydroxysteroid dehydrogenase/isomerase | - |
Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Rattus norvegicus | |
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Release 2023.1 (January 2023)
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