General Stability | Organism |
---|---|
NADP+ protects enzyme activity | Rattus sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3beta-hydroxy-11-oxo-18beta-olean-12-en-30-oic-acid | competitive inhibition, Ki: 4 nM; i.e. glycyrrhetinic acid | Rattus sp. | |
glycyrrhizic acid | competitive inhibition, Ki: 0.0005 mM | Rattus sp. | |
additional information | no end-product inhibition | Rattus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000196 | - |
NADP+ | reduction of corticosterone, ordered sequential mechanism | Rattus sp. | |
0.00183 | - |
11beta,21-dihydroxypregn-4-en-3,20-dione | - |
Rattus sp. | |
0.00355 | - |
NADPH | oxidation of cortisol | Rattus sp. | |
0.0173 | - |
11beta,17,21-trihydroxy-pregn-4-ene-3,20-dione | i.e. cortisol | Rattus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
11beta,17,21-trihydroxy-pregn-4-ene-3,20-dione + NADP+ | Rattus sp. | i.e. cortisol | 17,21-dihydroxy-pregn-4-ene-3,11,20-trione + NADPH | i.e. cortisone | ir | |
21-hydroxy-pregn-4-en-3,11,20-trione + NADPH | Rattus sp. | - |
11beta,21-dihydroxypregn-4-en-3,20-dione + NADP+ | i.e. corticosterone | ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus sp. | - |
Storage Stability | Organism |
---|---|
-20°C, 1 mM NADP+, several months | Rattus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
11beta,17,21-trihydroxy-pregn-4-ene-3,20-dione + NADP+ | i.e. cortisol | Rattus sp. | 17,21-dihydroxy-pregn-4-ene-3,11,20-trione + NADPH | i.e. cortisone | ir | |
21-hydroxy-pregn-4-en-3,11,20-trione + NADPH | - |
Rattus sp. | 11beta,21-dihydroxypregn-4-en-3,20-dione + NADP+ | i.e. corticosterone | ir |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Rattus sp. | |
NADPH | - |
Rattus sp. |