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Literature summary for 1.1.1.146 extracted from

  • Ogg, D.; Elleby, B.; Norstroem, C.; Stefansson, K.; Abrahmsen, L.; Oppermann, U.; Svensson, S.
    The crystal structure of guinea pig 11beta-hydroxysteroid dehydrogenase type 1 provides a model for enzyme-lipid bilayer interactions (2005), J. Biol. Chem., 280, 3789-3794.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of His6-tagged isozyme 11beta-HSD1 in Escherichia coli strain BL21(DE3) Cavia porcellus

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant isozyme 11beta-HSD1 in complex with substrate, cofactor and analogue, or inhibitor, 10 mg/ml protein in 20 mM Tris-HCl, pH 8.0, 2mM Tris(carboxyethyl)phosphine, 5% glycerol, 0.5 M guanidinum hydrochloride, 0.05% Anapoe X-100, 0.02 mM cortisone, and 0.04 mM NADP+ analogue 3-aminopyridine adenine dinucleotide phosphate AADP, NADP+, BVT-4584, or cortisone, hanging drop vapour diffusion method, mixing with equal volume of precipitation solution containing 38-40% PEG 550 mono methyl ether, and 0.1 M Bis-Tris, pH 6.5, equlibration against 1 ml precipitation solution, 5-6 days, X-ray diffraction structure determination and analysis at 2.5 A resolution Cavia porcellus

Inhibitors

Inhibitors Comment Organism Structure
BVT-4584
-
Cavia porcellus

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum the active site of isozyme 11beta-HSD1 is located on the lumen side Cavia porcellus 5783
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endoplasmic reticulum membrane
-
Cavia porcellus 5789
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additional information modeling of enzyme-bilayer interaction structure Cavia porcellus
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-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cortisone + NADPH + H+ Cavia porcellus isozyme 11beta-HSD1 catalyzes the forward reaction with cofactor NADPH cortisol + NADP+
-
ir

Organism

Organism UniProt Comment Textmining
Cavia porcellus Q6QLL4
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged isozyme 11beta-HSD1 from Escherichia coli strain BL21(DE3) by metal affinity chromatography Cavia porcellus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cortisone + NADPH + H+ isozyme 11beta-HSD1 catalyzes the forward reaction with cofactor NADPH Cavia porcellus cortisol + NADP+
-
ir

Synonyms

Synonyms Comment Organism
11beta-HSD1
-
Cavia porcellus
11beta-hydroxysteroid dehydrogenase type 1
-
Cavia porcellus

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Cavia porcellus