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Literature summary for 1.1.1.149 extracted from

  • Pongsawasdi, P.; Anderson, B.M.
    Inactivation of rat ovarian 20alpha-hydroxysteroid dehydrogenase by N-alkylmaleimides (1984), Arch. Biochem. Biophys., 233, 481-488.
    View publication on PubMed
Search for more literature for 1.1.1.149

Inhibitors

Inhibitors Comment Organism Structure
2,4-Pentanedione pH profile for the inactivation, overview Rattus norvegicus
3-Aminopyridine adenine dinucleotide phosphate diazotized, coenzyme-competitive at 25°C, pH 7.4, reversible binding Rattus norvegicus
Fluorescein mercuric acetate
-
 Rattus norvegicus
Maleimide pH profile for the inactivation, overview Rattus norvegicus
additional information apparent second-order rate constants for inactivation are observed to increase with increasing chain length of the N-alkylmaleimide used, NADP-and coenzyme-competitive inhibitors such as 3-aminopyridine adenine dinucleotide phosphate and various adenosine derivatives protect the enzyme against maleimide inactivation, whereas no protection is observed with the steroid substrate, overview; NADP+, 3-aminopyridine adenine dinucleotide phosphate and three adenosine derivatives protect enzyme against inactivation by N-ethylmaleimide, 20alpha-hydroxyprogesterone does not and N1-ethylnicotinamide chloride not significantly; sulfhydryl groups are modified during inactivation Rattus norvegicus
N-Alkylmaleimide N-ethylmaleimide and N-butylmaleimide to N-octylmaleimide at 25°C, pH 7.7 Rattus norvegicus
N-Butylmaleimide inactivation via sulfhydryl groups of the enzyme in a nonpolar region Rattus norvegicus
N-ethylmaleimide inactivation via sulfhydryl groups of the enzyme in a nonpolar region Rattus norvegicus
N-Heptylmaleimide inactivation via sulfhydryl groups of the enzyme in a nonpolar region Rattus norvegicus
N-Hexylmaleimide inactivation via sulfhydryl groups of the enzyme in a nonpolar region Rattus norvegicus
N-Octylmaleimide inactivation via sulfhydryl groups of the enzyme in a nonpolar region Rattus norvegicus
N-Pentylmaleimide inactivation via sulfhydryl groups of the enzyme in a nonpolar region Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
K+ activates Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
progesterone + NAD(P)H Rattus norvegicus
-
 20alpha-hydroxypregn-4-en-3-one + NAD(P)+
-
 r

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
ovary
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
20alpha-hydroxyprogesterone + NADP+
-
 Rattus norvegicus progesterone + NADPH
-
 ?
progesterone + NAD(P)H
-
 Rattus norvegicus 20alpha-hydroxypregn-4-en-3-one + NAD(P)+
-
 r
progesterone + NAD(P)H i.e. pregn-4-en-3-one, importance of sulfhydryl groups in the functioning of the enzyme Rattus norvegicus 20alpha-hydroxypregn-4-en-3-one + NAD(P)+
-
 r

Synonyms

Synonyms Comment Organism
20alpha-HSD
-
 Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.7
-
 assay at Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
NAD(P)+
-
 Rattus norvegicus
NAD(P)H
-
 Rattus norvegicus
NADP+
-
 Rattus norvegicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
 additional information inhibition kinetics Rattus norvegicus