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Literature summary for 1.1.1.156 extracted from
- Purification and properties of an NADPH-dependent dihydroxyacetone reductase from Phycomyces spores (1985), FEMS Microbiol. Lett., 30, 377-381.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| KCl | inhibition above 100 mM | Phycomyces blakesleeanus |  |
| Mg2+ | 40% inhibition at 5 mM | Phycomyces blakesleeanus | |
| Mn2+ | 25% inhibition at 5 mM | Phycomyces blakesleeanus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
NADPH | - |
Phycomyces blakesleeanus | |
| 0.167 | - |
NADP+ | - |
Phycomyces blakesleeanus | |
| 3.9 | - |
dihydroxyacetone | - |
Phycomyces blakesleeanus | |
| 2437 | - |
glycerol | - |
Phycomyces blakesleeanus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34000 | 39000 | gel filtration, SDS-PAGE | Phycomyces blakesleeanus |
| 39000 | - |
1 * 39000, SDS-PAGE | Phycomyces blakesleeanus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glycerol + NADP+ | Phycomyces blakesleeanus | involved in synthesis of glycerol during early spore germination | dihydroxyacetone + NADPH + H+ | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Phycomyces blakesleeanus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity, chromatography techniques | Phycomyces blakesleeanus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| sporangiospore | - |
Phycomyces blakesleeanus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 31.1 | - |
- |
Phycomyces blakesleeanus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dihydroxyacetonephosphate + NADPH | very low activity | Phycomyces blakesleeanus | glycerol 3-phosphate + NADP+ | - |
? | |
| glycerol + NADP+ | involved in synthesis of glycerol during early spore germination | Phycomyces blakesleeanus | dihydroxyacetone + NADPH + H+ | - |
r | |
| L-arabitol + NADP+ | 11% of the activity compared to glycerol | Phycomyces blakesleeanus | ? + NADPH | - |
? | |
| L-glyceraldehyde + NADPH | very low activity | Phycomyces blakesleeanus | L-glycerol + NADP+ | - |
? | |
| meso-erythritol + NADP+ | 35% of the activity compared to glycerol | Phycomyces blakesleeanus | ? + NADPH | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 39000, SDS-PAGE | Phycomyces blakesleeanus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 6.5 | dihydroxyacetone reduction | Phycomyces blakesleeanus |
| 9.4 | - |
glycerol oxidation | Phycomyces blakesleeanus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | can not be replaced by NAD+ | Phycomyces blakesleeanus | |
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